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Abstract Severe febrile illnesses in children encompass life-threatening organ dysfunction caused by diverse pathogens and other severe inflammatory syndromes. A comparative approach to these illnesses may identify shared and distinct features of host immune dysfunction amenable to immunomodulation. Here, using immunophenotyping with mass cytometry and cell stimulation experiments, we illustrate trajectories of immune dysfunction in 74 children with multi-system inflammatory syndrome in children (MIS-C) associated with SARS-CoV-2, 30 with bacterial infection, 16 with viral infection, 8 with Kawasaki disease, and 42 controls. We explore these findings in a secondary cohort of 500 children with these illnesses and 134 controls. We show that neutrophil activation and apoptosis are prominent in multi-system inflammatory syndrome, and that this is partially shared with bacterial infection. We show that memory T cells from patients with multi-system inflammatory syndrome and bacterial infection are exhausted. In contrast, we show viral infection to be characterized by a distinct signature of decreased interferon signaling and lower interferon receptor gene expression. Improved understanding of immune dysfunction may improve approaches to immunomodulator therapy in severe febrile illnesses in children.

In many cultural contexts, judging another as conscious or not has profound practical, legal, and philosophical consequences. However, little research focuses on how our ability to make such judgements arises. Thirty years ago a classic set of studies by Flavell et al. demonstrated that children do not develop a complex understanding of conscious ideation in others until age 8. We investigated whether children understand a more basic aspect of conscious experience at earlier ages: a person has conscious thoughts when awake in contrast to when soundly, dreamlessly asleep. We tested 211 3-to-13-year-old children, who watched video sequences, focally one where a person was asleep and then awoke. Children were half as likely to say the target was having thoughts while asleep compared to awake. Even the youngest children made this distinction, demonstrating that children as young as 3-years have at least a basic understanding of consciousness in others.

Abstract Microglia are important players in surveillance and repair of the brain. Implanting an electrode into the cortex activates microglia, produces an inflammatory cascade, triggers the foreign body response, and opens the blood-brain barrier. These changes can impede intracortical brain-computer interfaces performance. Using two-photon imaging of implanted microelectrodes, we test the hypothesis that low-intensity pulsed ultrasound stimulation can reduce microglia-mediated neuroinflammation following the implantation of microelectrodes. In the first week of treatment, we found that low-intensity pulsed ultrasound stimulation increased microglia migration speed by 128%, enhanced microglia expansion area by 109%, and a reduction in microglial activation by 17%, indicating improved tissue healing and surveillance. Microglial coverage of the microelectrode was reduced by 50% and astrocytic scarring by 36% resulting in an increase in recording performance at chronic time. The data indicate that low-intensity pulsed ultrasound stimulation helps reduce the foreign body response around chronic intracortical microelectrodes.

Meniscus tissue is commonly injured due to sports-related injuries and age-related degeneration and approximately 50% of individuals with a meniscus tear will develop post-traumatic osteoarthritis (PTOA). Given that the meniscus has limited healing potential, new therapeutic strategies are required to enhance meniscus repair. Porcine meniscus-derived matrix (MDM) scaffolds improve meniscus integrative repair, but sources of human meniscus tissue have not been investigated. Therefore, the objectives of this study were to generate healthy and osteoarthritic (OA) MDM scaffolds and to compare meniscus cellular responses and integrative repair. Meniscus cells showed high viability on both healthy and OA scaffolds. While DNA content was higher in cell-seeded OA scaffolds than cell-seeded healthy scaffolds, CCK-8, and both sGAG and collagen content were similar between scaffold types. After 28 days in an ex vivo meniscus defect model, healthy and OA scaffolds had similar DNA, sGAG, and collagen content. However, the shear strength of repair was reduced in defects containing OA scaffolds compared to healthy scaffolds. In conclusion, healthy human allograft tissue is a useful source for generating MDM scaffolds that can support cellular growth, ECM production, and ex vivo integrative repair of the meniscus, highlighting the potential suitability for tissue engineering approaches to improve meniscus repair.

The Academy of Management Journal (AMJ) provides a broad platform for empirical management research that encompasses diverse topics, methodologies, and data types. Despite the wide array of studies published, successful research projects share key elements, such as an intriguing research question, a structured theoretical foundation, and a suitable research setting. This From the Editors piece introduces the AMJ Management Research Canvas, a guide for scholars to systematically address these core elements. By helping researchers align their studies with essential design and theoretical criteria, the canvas aims to improve the quality and coherence of submissions, benefiting both novice and experienced scholars in management and organizational research.

IntroductionLong-term availability of health-promoting interventions (HPIs) in school settings can translate into health benefits for children. However, little is known about factors associated with HPI institutionalization in schools. In this study, we identified correlates of the institutionalization of HPIs offered in elementary schools in Quebec, Canada. MethodsIn two-part, structured telephone interviews over three academic years (2016–2019), elementary school principals (or their designees) throughout Quebec identified an index HPI offered at least once in their school during the previous three years, and were asked whether it was institutionalized (i.e. explicitly written in the school’s educational project, e.g. in the form of educational objectives and means of achieving them). We examined associations between institutionalization and 10 school-related and 16 HPI-related characteristics in univariable and multivariable logistic regression analyses. ResultsSchool key informants (n = 163) reported on 147 different HPIs that had been available in their schools in the past three years, 56% of which were institutionalized. Three aspects of school culture—parent/community engagement with the school, school/teacher commitment to student health and school physical environment—were positively associated with HPI institutionalization. HPI-related characteristics positively associated with HPI institutionalization included number of competencies addressed by the HPI, number of teaching strategies employed, modifications made to the HPI prior to or during implementation and perceived success of the HPI. Inviting families or community groups to participate in the HPI was inversely associated with institutionalization. ConclusionBetter understanding of factors associated with HPI institutionalization may inform the development of school-based HPIs that have the potential for sustainability.

IntroductionL’offre à long terme d’interventions de promotion de la santé (IPS) en milieu scolaire peut se révéler bénéfique pour la santé des enfants. Toutefois, on en sait peu sur les facteurs associés à cette institutionnalisation dans les écoles. Dans cette étude, nous avons dégagé les corrélats de l’institutionnalisation des IPS offertes dans les écoles primaires du Québec (Canada). MéthodologieDans le cadre d’entrevues téléphoniques structurées en deux parties réalisées sur trois années scolaires (2016-2019), des directeurs et directrices d’écoles primaires (ou leurs représentants désignés) du Québec devaient choisir une IPS de référence ayant été offerte au moins une fois dans leur école dans les trois années précédentes. Ils devaient ensuite indiquer si l’IPS avait été institutionnalisée (c.-à-d. si elle figurait explicitement dans le projet éducatif de l’école, par exemple sous la forme d’objectifs éducatifs accompagnés des mesures permettant leur atteinte). Nous avons analysé les associations entre, d’une part, l’institutionnalisation des IPS et, d’autre part, dix caractéristiques liées aux écoles et seize caractéristiques liées aux IPS à l’aide d’analyses de régression logistique univariées et multivariées. RésultatsLes informateurs clés des écoles (n = 163) ont fait état de 147 IPS différentes ayant été offertes dans leur école dans les trois années précédentes et 56 % de ces IPS avaient été institutionnalisées. Trois dimensions de la culture de l’école – soit 1) la participation des parents et de la collectivité à la vie scolaire, 2) l’engagement de l’école et du personnel enseignant à l’égard de la santé des élèves et 3) l’environnement physique de l’école – étaient associées positivement à l’institutionnalisation des IPS. En ce qui a trait aux caractéristiques liées aux IPS, ce sont le nombre de compétences visées par celles-ci, le nombre de stratégies d’enseignement utilisées, les modifications apportées à l’IPS avant ou pendant sa mise en oeuvre et le succès perçu de l’IPS qui ont présenté une association positive avec l’institutionnalisation des IPS. Le fait d’inviter les familles ou les groupes communautaires à participer à l’IPS était inversement associé à l’institutionnalisation. ConclusionUne meilleure compréhension des facteurs liés à l’institutionnalisation des IPS pourrait éclairer l’élaboration d’IPS présentant un potentiel de durabilité en milieu scolaire.

Abstract Regional pulmonary perfusion (Q) has been investigated using blood volume (Fb) imaging as an easier-to-measure surrogate. However, it is unclear if changing pulmonary conditions could affect their relationship. We hypothesized that vascular changes in early acute respiratory distress syndrome (ARDS) affect Q and Fb differently. Five sheep were anesthetized and received lung protective mechanical ventilation for 20 h while endotoxin was continuously infused. Using dynamic 18F-FDG and 13NN Positron Emission Tomography (PET), regional Fb and Q were analysed in 30 regions of interest (ROIs) and normalized by tissue content (Fbn and Qn, respectively). After 20 h, the lung injury showed characteristics of early ARDS, including gas exchange and lung mechanics. PET images of Fbn and Qn showed substantial differences between baseline and lung injury. Lung injury caused a significant change in the Fbn-Qn relationship compared to baseline (p

Hinge constructs can be used in revision total knee arthroplasty in cases of major collateral ligament deficiency, global knee instability, repeated failure of constrained implants, or large bone defects. Although the success of this construct in revision total knee arthroplasty varies depending on the type of surgery and bone quality, hinged implants can solve a wide array of complex problems while preserving stable flexion-extension motion for ambulation. The most common reasons for re-revision include patellofemoral complications, aseptic loosening, and infection. Most cases of mechanical failure for these hinged constructs occur at the hinge component. Here, we report the first instance of catastrophic femoral component failure in a modular rotating hinge via a low-energy mechanism.

Thermogenesis by uncoupling protein 1 (UCP1) is one of the primary mechanisms by which brown adipose tissue (BAT) increases energy expenditure. UCP1 resides in the inner mitochondrial membrane (IMM), where it dissipates membrane potential independent of adenosine triphosphate (ATP) synthase. Here, we provide evidence that phosphatidylethanolamine (PE) modulates UCP1-dependent proton conductance across the IMM to modulate thermogenesis. Mitochondrial lipidomic analyses revealed PE as a signature molecule whose abundance bidirectionally responds to changes in thermogenic burden. Reduction in mitochondrial PE by deletion of phosphatidylserine decarboxylase (PSD) made mice cold intolerant and insensitive to β3 adrenergic receptor agonist-induced increase in whole-body oxygen consumption. High-resolution respirometry and fluorometry of BAT mitochondria showed that loss of mitochondrial PE specifically lowers UCP1-dependent respiration without compromising electron transfer efficiency or ATP synthesis. These findings were confirmed by a reduction in UCP1 proton current in PE-deficient mitoplasts. Thus, PE performs a previously unknown role as a temperature-responsive rheostat that regulates UCP1-dependent thermogenesis.

Two capacities considered foundational in human cooperation are prosocial motivation and goal-reading abilities that enable helping. Children exhibit both proclivities by age 2 in interactions with other humans, but interactions with nonhuman species on whom we have been interdependent for millennia are unstudied. We tested the hypothesis that children’s goal-reading and prosocial propensities extend to other animals. We predicted children would help pet dogs access objects that dogs attempted to reach but could not reach themselves. We studied 97 children between 2 and 3 years of age living in a small mid-western US city, 44 of whom had dogs as household pets. In a quasi-naturalistic setting, we introduced children to 1 of 3 friendly pet dogs who remained within a small, porous enclosure while a treat or toy was placed outside it. Dogs reacted naturally, either showing interest in accessing the item (e.g., pawing, begging) or ignoring it. Measures of dog and child behavior during sessions were coded blindly with high reliability. Children provided dogs with out-of-reach items twice as often when dogs showed interest rather than ignored items, indicating sensitivity to the dog’s goals. Additionally, children were more generally likely to provide dogs with items if children lived with pet dogs, if dogs were more lively and engaged rather than subdued and if the item was a treat rather than a toy. These findings lend support to our hypothesis that children’s early-developing proclivities for goal-reading and prosociality extend beyond humans to other animals.

To examine how social media influencers aid in the growth of the wellness industry while simultaneously reifying dangerous existing ideologies, we conducted a thematic analysis of TikTok videos created by a social media influencer during her time at a popular wellness tourism retreat known as The Ranch Malibu. In the findings, we outline how wellness tourism discourse promotes extreme dieting, the moralization of health, and elitism, all outcomes in line with previous studies explicating the dangers of both contemporary and historical wellness rhetoric. We argue that the sharing of wellness tourism content on social media platforms results in the spread of dangerous beliefs alongside health misinformation at a much faster pace than in decades prior. Therefore, we encourage communication scholars to examine wellness tourism discourse on social media platforms as a novel research area within the growing fields of digital health communication, rhetoric of health and wellness, and public health.

Background:. Exposure to ultraviolet radiation is a leading risk factor for developing all types of skin cancer. In the United States, an estimated 7.8 million young adults engage in indoor tanning. Objective:. Here, it is hypothesized that certain populations of students at undergraduate universities, namely sorority members, have a greater frequency of tanning bed usage than other groups of students and that regardless of sorority status, the most important motivating factor will be the intent to enhance one’s appearance. Methods:. Undergraduate students at 2 state-funded universities were recruited for participation in this institutional review board-exempt survey via distribution to e-mail addresses and social media accounts affiliated with student organizations/clubs. Results:. Among all respondents, the most common motivating factors for tanning bed use were the perception of improved self-appearance and boosted self-confidence. Female sorority members were more likely to use tanning beds and also more likely to report being motivated by enhanced appearance and self-confidence, than their female counterparts who were not sorority members. Limitations:. The sample size (n = 321) and population of this study allows data to only be generalizable to surrounding states with similar demographics. The findings of this study are subject to recall bias as the data is self-reported. Conclusion:. Tanning bed use remains a popular practice among young people. Understanding motivations for tanning bed use among populations at increased risk of partaking in this behavior allows for educating these groups on the risks associated with ultraviolet radiation exposure. It is crucial that providers continue to promote skin health and take steps to dissuade detrimental practices and possible habit-forming behaviors at the individual and state levels.