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231 results on '"Wen, Guoyuan"'

32. Characterization of a Recombinant Thermostable Newcastle Disease Virus (NDV) Expressing Glycoprotein gB of Infectious Laryngotracheitis Virus (ILTV) Protects Chickens against ILTV Challenge

Author

Zeng, Zhe, primary, He, Yan, additional, Wang, Zichen, additional, Yao, Lun, additional, Li, Li, additional, Shang, Yu, additional, Wang, Hongcai, additional, Zhang, Rongrong, additional, Shao, Huabin, additional, Luo, Qingping, additional, and Wen, Guoyuan, additional

Published
2023
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35. Flos populi (Male Inflorescence of Populus tomentosa Carrière) Aqueous Extract Suppresses Salmonella Pullorum Infection by Affecting T3SS-1.

Author

Zhang, Wenting, Liang, Guixing, Cheng, Zhenyu, Guo, Yunqing, Jiang, Boda, Liu, Tingjiang, Liao, Weidong, Lu, Qin, Wen, Guoyuan, Zhang, Tengfei, and Luo, Qingping

Subjects
SALMONELLA diseases, POULTRY diseases, INTESTINAL diseases, POULTRY industry, COMMUNICABLE diseases
Abstract

Pullorum disease, caused by Salmonella Pullorum (S. Pullorum), is one of the most serious infectious diseases in the poultry industry. Flos populi is traditionally used in Eastern Asian countries to treat various intestinal diseases. However, the anti-infection mechanism of Flos populi is not very clear. In this study, we evaluated the anti-infective effects on S. Pullorum of Flos populi aqueous extract (FPAE) in chickens. FPAE significantly reduced S. Pullorum growth in vitro. At the cellular level, FPAE reduced S. Pullorum adhesion and invasion on DF-1 cells but did not affect its intracellular survival or replication in macrophages. Further investigation revealed that FPAE inhibited the transcription of T3SS-1 genes, which is the main virulence factor that mediates S. Pullorum adhesion and invasion in host cells. The results suggest that the anti-infective effect of FPAE likely occurs through the inhibition of S. Pullorum T3SS-1, thereby impairing its ability to adhere to and invade cells. Further, we evaluated its therapeutic effect on animal models (Jianghan domestic chickens) and found that FPAE reduced the bacterial loads in organs and decreased the mortality and weight loss of infected chickens. Our findings provide novel insights into the potential development of FPAE against S. Pullorum as an effective anti-virulence therapeutic substitute for antibiotics. [ABSTRACT FROM AUTHOR]

Published
2023
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37. Quantitative regulation of the thermal stability of enveloped virus vaccines by surface charge engineering to prevent the self-aggregation of attachment glycoproteins

Author

Shang, Yu, primary, Li, Li, additional, Zhang, Tengfei, additional, Luo, Qingping, additional, Yu, Qingzhong, additional, Zeng, Zhe, additional, Li, Lintao, additional, Jia, Miaomiao, additional, Tang, Guoyi, additional, Fan, Sanlin, additional, Lu, Qin, additional, Zhang, Wenting, additional, Xue, Yuhan, additional, Wang, Hongling, additional, Liu, Wei, additional, Wang, Hongcai, additional, Zhang, Rongrong, additional, Ding, Chan, additional, Shao, Huabin, additional, and Wen, Guoyuan, additional

Published
2022
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40. Evaluation of young chickens challenged with aerosolized Salmonella Pullorum

Author

Zhang Sihua, Wen Guoyuan, Yiluo Cheng, Qingping Luo, Zhang Tengfei, Shao Huabin, Zhang Wenting, and Lu Qin

Subjects
Serotype, General Immunology and Microbiology, biology, 040301 veterinary sciences, business.industry, Transmission (medicine), animal diseases, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Poultry farming, biology.organism_classification, Pathogenicity, 040201 dairy & animal science, Microbiology, 0403 veterinary science, Food Animals, Salmonella enterica, Salmonella Pullorum, Animal Science and Zoology, business, Pathogen, Aerosolization
Abstract

Salmonella enterica serovar Pullorum (S. Pullorum) is an important pathogen specific to avian species, which poses a serious threat to the poultry industry. The transmission of S. Pullorum occurs b...

Published
2020

41. In-ovo Newcastle disease virus vaccine strain TS09-C protects commercial chickens against Newcastle disease in the presence of maternally derived antibodies

Author

Sanling Fan, Luo Qingping, Shang Yu, Huabin Shao, Wen Guoyuan, Zhang Tengfei, Luo Ling, Zhang Wenting, Yulun Wu, Zhang Rongrong, and Hongling Wang

Subjects
Cellular immunity, animal structures, Newcastle Disease, animal diseases, medicine.medical_treatment, Newcastle disease virus, Passive immunity, Biology, Antibodies, Viral, In ovo, Newcastle disease, Virus, Injections, 03 medical and health sciences, Immune system, medicine, Animals, Poultry Diseases, lcsh:SF1-1100, Ovum, 030304 developmental biology, maternally derived antibody, 0303 health sciences, Vaccination, 0402 animal and dairy science, Viral Vaccines, 04 agricultural and veterinary sciences, General Medicine, Immunology, Health and Disease, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, 040201 dairy & animal science, Virology, in-ovo vaccine, embryonic structures, biology.protein, Animal Science and Zoology, lcsh:Animal culture, Antibody, Chickens, strain TS09-C
Abstract

Maternally derived antibodies (MDA) substantially interfere with active immunity in post-hatch vaccination, although they provide early protection against disease through passive immunity in young chickens. Previously, Newcastle disease virus (NDV) strain TS09-C was demonstrated to be safe and immunogenic as in-ovo vaccine in specific-pathogen-free chickens. Here, we evaluated the safety, protective efficacy, and duration of clinical protection of the TS09-C virus as an in-ovo vaccine for commercial chickens in the presence of Maternally derived antibodies against NDV. This vaccine was safe in commercial chickens and provided at least 80% protection against a virulent NDV challenge for 3 mo, despite inducing a low hemagglutinin-inhibition titer. For commercial chickens, the protective efficacy of the in-ovo vaccination was markedly higher than that of posthatch vaccination, and the cellular immune response might play an important role in the higher protective efficacy of the in-ovo vaccine. The overall results indicate that the maternally derived antibodies against NDV do not significantly interfere with the ability of the in-ovo vaccine strain TS09-C to induce protective cellular immunity.

Published
2020

42. High concentration of coagulase-negative staphylococci carriage among bioaerosols of henhouses in Central China

Author

Yiluo Cheng, Wen Guoyuan, Lu Qin, Qingping Luo, Shao Huabin, Yuanqing Lu, and Zhang Tengfei

Subjects
Microbiology (medical), China, genetic structures, Inflammatory cytokine, Antibiotic resistance, Staphylococcus, Indoor bioaerosol, lcsh:QR1-502, Biology, Microbiology, lcsh:Microbiology, 03 medical and health sciences, Drug Resistance, Bacterial, Staphylococcus sciuri, Animals, Humans, mecA, Pathogen, 030304 developmental biology, Oxacillin, Bioaerosol, 0303 health sciences, Farmers, 030306 microbiology, Interleukins, cons, Coagulase-negative staphylococci, biology.organism_classification, Housing, Animal, Nasal Mucosa, Parasitology, sense organs, Coagulase, Chickens, Research Article
Abstract

Background Coagulase-negative staphylococci (CoNS) are a group of opportunistic pathogens, which are widely spread in the environment. Animal breeding is an important source of pathogen spreading. However, the concentration and characteristics of CoNS in the bioaerosols of henhouses are unclear. Results In this study, we showed that CoNS were significantly increased in bioaerosols of henhouses during the first 60 days, and reached 2.0 × 106 CFU/m3, which account for 75.4% of total bacteria. One hundred and two CoNS isolates from bioaerosols and nasal swabs of farmers were further identified, covering seven species. Among these, 41.2% isolates were Staphylococcus sciuri, which was the predominant species, followed by S. equorum, S. saprophyticus, S. haemolyticus, S. xylosus, S. arlettae and S. gallinarum. There were high rates of resistance to oxacillin in CoNS (49.0%), which were defined as Methicillin-Resistant CoNS (MRCoNS), and 36.3% isolates contained resistance gene mecA. Bioaerosol infection models showed that, chickens exposed to aerosolized S. sciuri had significant induction of inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8 and IL-10 at 5 days post-infection (dpi) in lungs and at 7 dpi in spleens. Conclusions We reported a high concentration of CoNS in henhouses, and S. sciuri was the preponderant CoNS species. Antibiotic resistance analysis and bioaerosols infection of CoNS further highlighted its hazards on resistance and immunological challenge. These results suggested that, CoNS in bioaerosols could be one serious factor in the henhouses for not only poultry industry but also public health.

Published
2020

46. Molecular characterization and functional analysis of duck CCCH-type zinc finger antiviral protein (ZAP)

Author

Huabin Shao, Yan He, Liurong Fang, Zhang Tengfei, Lu Qin, Shudan Liu, Shaobo Xiao, Wen Guoyuan, Qingping Luo, Rongrong Zhang, and Xinyu Zhu

Subjects
0301 basic medicine, Poly ADP ribose polymerase, Biophysics, Clone (cell biology), Biology, Biochemistry, Avian Proteins, 03 medical and health sciences, 0302 clinical medicine, Complementary DNA, Animals, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Transcription factor, Polymerase, Cells, Cultured, Phylogeny, Gene knockdown, Innate immune system, NF-kappa B, RNA-Binding Proteins, Zinc Fingers, Cell Biology, Interferon-beta, Fibroblasts, Molecular biology, Immunity, Innate, 030104 developmental biology, IRF1, Ducks, 030220 oncology & carcinogenesis, biology.protein, Sequence Alignment, Interferon Regulatory Factor-1, Signal Transduction
Abstract

This is the first study to clone duck CCCH-type zinc finger antiviral protein (duZAP) from Jingjiang duck (Anas platyrhynchos). Full-length duZAP cDNA was 2154 bp and encoded a 717-amino acid polypeptide containing four highly conserved CCCH-type finger motifs, a WWE domain and a poly (ADP-ribose) polymerase (PARP) domain. duZAP was expressed in multiple duck tissues, with the highest mRNA expression in the spleen. Overexpression of duZAP in duck embryo fibroblast cells (DEFs) led to activation of the transcription factors IRF1 and NF-κB, and induction of IFN-β. Analysis of deletion mutants revealed that both the WWE and PARP domains of duZAP were essential for activating the IFN-β promoter. Knockdown of duZAP in DEFs significantly reduced poly (I:C)- and duck Tembusu virus (DTMUV)-induced IFN-β activation. Our findings further the understanding of the role of duZAP in the duck innate immune response.

Published
2021

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