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17 results on '"Wen, Yi-min"'

2. A Confident Majority Voting Strategy for Parallel and Modular Support Vector Machines

Author

Wen, Yi-Min, Lu, Bao-Liang, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Rangan, C. Pandu, editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Doug, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Liu, Derong, editor, Fei, Shumin, editor, Hou, Zengguang, editor, Zhang, Huaguang, editor, and Sun, Changyin, editor

Published
2007
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4. A Cascade Method for Reducing Training Time and the Number of Support Vectors

Author

Wen, Yi-Min, Lu, Bao-Liang, Hutchison, David, editor, Kanade, Takeo, editor, Kittler, Josef, editor, Kleinberg, Jon M., editor, Mattern, Friedemann, editor, Mitchell, John C., editor, Naor, Moni, editor, Nierstrasz, Oscar, editor, Pandu Rangan, C., editor, Steffen, Bernhard, editor, Sudan, Madhu, editor, Terzopoulos, Demetri, editor, Tygar, Dough, editor, Vardi, Moshe Y., editor, Weikum, Gerhard, editor, Yin, Fu-Liang, editor, Wang, Jun, editor, and Guo, Chengan, editor

Published
2004
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17. [Preliminary study on cryopreservation of tissue engineered bone at -8 degrees C].

Author

Lan X, Wen YM, Ge BF, and Liu XM

Subjects
Animals, Cell Adhesion, Cell Survival, Flow Cytometry, Humans, Mesenchymal Stem Cells cytology, Rabbits, Bone and Bones cytology, Cryopreservation, Tissue Engineering
Abstract

Objective: To study the effects of various methods of cryopreservation on the bioactivity of tissue engineered bone., Methods: MSCs were cocultured with partialy deproteinised bone to produce tissue engineered bone. The experiment was divided into A, B, C and D group. Group A: Tissue engineered bone was stored in preservation solution with cryopreservation medium. Group B: Tissue engineered bone was stored in preservation solution without cryopreservation medium. Group C: Tissue engineered bone was stored without cryopreservation. Group D: MSCs were cultured without cryopreservation. The tissue engineered bone of group A and B had been cryopreserved at -80 degrees C for three months and thawed three months later. The electronic scanning microscope was used to evaluate the adhesion and distribution of MSCs, cell viability was measured by MTT, ALP activity was detected by p-nitrophosphate, cell cycle was analysed by flow cytometry., Results: MSCs could adhere to the surface of the material and distribute in the hole of material. The cell viability of MSCs adhered to the material was C > A > B group (P < 0.01, P < 0.05). The ALP activity of MSCs adhered to material was C > A > B group (P < 0.01). The cell cycles of different groups did not change significantly; the abnormal cells were not observed., Conclusion: The choice of proper cryopreservative solution could optimize the bioactivity of tissue engineered bone.

Published
2008

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