153 results on '"Wen Ting Li"'
Search Results
2. High-resolution 3T magnetic resonance imaging and histological analysis of capsuloligamentous complex of the first metatarsophalangeal joint
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Jin-e Wang, Rong-jie Bai, Hui-li Zhan, Wen-ting Li, Zhan-hua Qian, Nai-li Wang, and Yuming Yin
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Anatomy ,Magnetic resonance imaging ,Histological analysis ,Capsuloligamentous complex ,First metatarsophalangeal joint ,Foot ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background There are discrepancies in the understanding of the structure of the capsuloligamentous complex of the first metatarsophalangeal joint (MTPJ); this study aims to investigate the differences with previous anatomical reports of high-resolution 3T magnetic resonance imaging (MRI) and histological analysis in illustrating the structure of the capsuloligamentous complex of the first MTPJ. Methods Nine fresh frozen cadaveric feet specimens (from two women and three men; aged 32 to 58 years) were used in this study. All specimens underwent MR examination with T1-weighted imaging and T2-weighted spectral attenuated inversion recovery in three planes. Subsequently, all cadaveric feet specimens were sliced into 2-mm-thick sections. The MRI features of the capsuloligamentous complex of the first MTPJ were analyzed in these specimens. Hematoxylin–eosin and Masson’s trichrome staining methods were used to explore the histologic features of the capsuloligamentous complex of the first MTPJ. Results Different from most previous studies, our results showed that the plantar plate could be divided into four portions including the central portion of the plantar plate, the intersesamoid, the sesamoid phalangeal and the metatarsosesamoid ligaments. The normal central portion of the plantar plate could be clearly visualized in the sagittal and coronal plane MR images. The intersesamoid ligament is a continuation of the central portion of the plantar plate on the sagittal plane on the gross specimen, the MR imaging, and the histological examination. On the coronal plane of the gross specimen and MR imaging, the sesamoid phalangeal ligaments and the central portion of the plantar plate can be seen as separate ligaments, but they appeared interwoven with the same continuous collagenous fibers on the histological analysis. Conclusion High-resolution 3T MRI allows accurate demonstration of the different anatomical details of the capsuloligamentous complex of the first MTPJ from previous anatomical reports. The histological analysis provides further understanding of the structures of the capsuloligamentous complex of the first MTPJ from previous studies.
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- 2021
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3. Training Method and Device of Chemical Industry Chinese Language Model Based on Knowledge Distillation.
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Wen-Ting Li, Shang-Bing Gao, Jun-Qiang Zhang, and Shu-Xing Guo
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- 2021
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4. CircFNDC3B sequestrates miR‐937‐5p to derepress TIMP3 and inhibit colorectal cancer progression
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Wei Zeng, Yi Liu, Wen‐Ting Li, Yi Li, and Jin‐Feng Zhu
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angiogenesis ,circFNDC3B ,colorectal cancer ,liver metastasis ,miR‐937‐5p ,TIMP3 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Circular RNA (circRNA) are single‐stranded RNA with covalently closed 3′ and 5′ ends, with many recognized to be involved in human diseases as gene regulators, typically by interacting with other RNA. CircFNDC3B is a circRNA formed by back‐splicing of exons 5 and 6 of the FNDC3B gene. CircFNDC3B was recently implicated in renal carcinoma, gastric and bladder cancer. However, the expression levels of circFNDC3B and its role in colorectal cancer (CRC) remain unclear. Expression of circFNDC3B and TIMP3 levels in CRC tissues and cell lines were found to be low, whereas microRNA (miR)‐937‐5p expression was high in CRC. MicroRNA‐937‐5p downregulated TIMP3, thereby promoting tumor cell proliferation, invasion, migration and angiogenesis. Moreover, CircFNDC3B was shown to bind to miR‐937‐5p. CircFNDC3B and circFNDC3B‐enriched exosomes inhibited tumorigenic, metastatic and angiogenic properties of CRC, and miR‐937‐5p overexpression or TIMP3 knockdown could reverse these effects. In vivo CRC tumor growth, angiogenesis and liver metastasis were suppressed by circFNDC3B overexpression, circFNDC3B‐enriched exosomes or miR‐937‐5p knockdown. In conclusion, our work reports a tumor‐suppressing role for the circFNDC3B–miR‐97‐5p–TIMP3 pathway and suggests that circFNDC3B‐enriched exosomes can inhibit angiogenesis and CRC progression.
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- 2020
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5. MiR-526b-3p Attenuates Breast Cancer Stem Cell Properties and Chemoresistance by Targeting HIF-2α/Notch Signaling
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Jing-Hua Liu, Wen-Ting Li, Yue Yang, Yan-Bo Qi, Yu Cheng, and Jia-Hui Wu
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breast cancer ,hypoxia ,CSCs ,chemoresistance ,miR-526b-3p ,HIF-2α ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Chemoresistance is a severe clinical challenge in breast cancer. Hypoxia and cancer stem cells (CSCs) contribute to the paclitaxel (PTX) resistance, but the molecular mechanisms are still elusive. MicorRNAs (miRNA) have been considered a promising therapeutic strategy in various cancers. Here, we identified the crucial function of miR-526b-3p in regulating PTX resistance and CSC properties. Our data demonstrated that miR-526b-3p mimic repressed the cell viability of breast cancer cells. The counts of Edu-positive cells were reduced by miR-526b-3p in breast cancer cells. Meanwhile, the apoptosis of breast cancer cells was induced by miR-526b-3p. Tumorigenicity analysis in the nude mice confirmed that miR-526b-3p attenuated the breast cancer cell growth in vivo. Significantly, hypoxia could enhance IC50 value of PTX in breast cancer cells. IC50 value of PTX was induced in breast cancer mammospheres. The hypoxia-inducible factor 2α (HIF-2α) expression was enhanced, but miR-526b-3p expression was repressed under hypoxia in breast cancer cells. Also, breast cancer mammospheres presented high HIF-2α expression and low miR-526b-3p expression. The inhibition of miR-526b-3p enhanced the IC50 value of PTX in breast cancer cells. MiR-526b-3p inhibitor enhanced the colony formation counts of PTX-treated breast cancer cells. The treatment of miR-526b-3p mimic suppressed the sphere formation counts of breast cancer cells and inhibited ALDH1 and Nanog expression. MiR-526b-3p was able to target HIF-2α in the cells. The overexpression enhanced but miR-526b-3p reduced the IC50 value of PTX in breast cancer cells, in which the overexpression of HIF-2α could rescue the miR-526b-3p-inhibited IC50 value of PTX. Overexpression of HIF-2α reversed miR-526b-3p-regulated apoptosis, colony formation ability, and ALDH1 and Nanog expression in the cells. Interestingly, the overexpression of HIF-2α induced but miR-526b-3p repressed the expression of HIF-2α, Hey2, and Notch in PTX-treated breast cancer cells, while HIF-2α could reverse the effect of miR-526b-3p. In conclusion, miR-526b-3p attenuated breast cancer stem cell properties and chemoresistance by targeting HIF-2α/Notch signaling. MiR-526b-3p may be utilized in the relieving chemoresistance in breast cancer.
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- 2021
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6. Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress
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Xiao-Xue Hou, Yu-Wen Li, Jia-Li Song, Wen Zhang, Rui Liu, Hui Yuan, Tian-Tong Feng, Zheng-Yi Jiang, Wen-Ting Li, and Chuan-Long Zhu
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Gastroenterology ,General Medicine - Published
- 2023
7. Identification of differentially expressed genes and pathways between intramuscular and abdominal fat-derived preadipocyte differentiation of chickens in vitro
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Meng Zhang, Fang Li, Xiang-fei Ma, Wen-ting Li, Rui-rui Jiang, Rui-li Han, Guo-xi Li, Yan-bin Wang, Zi-yi Li, Ya-dong Tian, Xiang-tao Kang, and Gui-rong Sun
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Chickens ,Abdominal fat ,Intramuscular fat ,Adipocyte differentiation ,Transcriptome ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Intramuscular fat (IMF) content is an important factor associated with meat quality, while abdominal fat (AbF) is regarded as one of the main factors affecting poultry slaughter efficiency. To investigate the differentially expressed genes (DEGs) and molecular regulatory mechanisms related to adipogenic differentiation between IMF- and AbF-derived preadipocytes, we analysed the mRNA expression profiles in preadipocytes (0d, Pre-) and adipocytes (10d, Ad-) from IMF and AbF of Gushi chickens. Results AbF-derived preadipocytes exhibited a higher adipogenic differentiation ability (96.4% + 0.6) than IMF-derived preadipocytes (86.0% + 0.4) (p
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- 2019
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8. 3-N-Butylphthalide mitigates high glucose-induced injury to Schwann cells: association with nitrosation and apoptosis
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Dan-Dan Xu, Wen-Ting Li, Dan Jiang, Huai-Guo Wu, Ming-Shan Ren, Mei-Qiao Chen, and Yuan-Bo Wu
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nerve regeneration ,Schwann cells ,3-n-butylphthalide ,3-nitrotyrosine ,nitration stress ,uric acid ,peroxynitrite anions ,diabetic peripheral neuropathy ,apoptosis ,proliferation ,neural regeneration ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
A high glucose state readily causes peripheral axon atrophy, demyelination, loss of nerve fiber function, and delayed regeneration. However, few studies have examined whether nitration is also critical for diabetic peripheral neuropathy. Therefore, this study investigated the effects of high glucose on proliferation, apoptosis, and 3-nitrotyrosine levels of Schwann cells treated with butylphthalide. In addition, we explored potential protective mechanisms of butylphthalide on peripheral nerves. Schwann cells were cultured in vitro with high glucose then stimulated with the peroxynitrite anion inhibitors uric acid and 3-n-butylphthalide for 48 hours. Cell Counting Kit-8 and flow cytometry were used to investigate the effects of uric acid and 3-n-butylphthalide on proliferation and apoptosis of Schwann cells exposed to a high glucose environment. Effects of uric acid and 3-n-butylphthalide on levels of 3-nitrotyrosine in Schwann cells were detected by enzyme-linked immunosorbent assay. The results indicated that Schwann cells cultured in high glucose showed decreased proliferation, but increased apoptosis and intracellular 3-nitrotyrosine levels. However, intervention with uric acid or 3-n-butylphthalide could increase proliferation of Schwann cells cultured in high glucose, and inhibited apoptosis and intracellular 3-nitrotyrosine levels. According to our data, 3-n-butylphthalide may inhibit cell nitrification and apoptosis, and promote cell proliferation, thereby reducing damage to Schwann cells caused by high glucose.
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- 2019
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9. Sports Injury-Related Fingers and Thumb Deformity Due to Tendon or Ligament Rupture
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Rong-Jie Bai, Hui-Bo Zhang, Hui-Li Zhan, Zhan-Hua Qian, Nai-Li Wang, Yue Liu, Wen-Ting Li, and Yu-Ming Yin
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Boutonniere Deformity ,Fingers ,Jersey Finger ,Magnetic Resonance Imaging ,Mallet Finger ,Stener Lesion ,Thumb ,Medicine - Abstract
Background: Hand injuries are very common in sports, such as skiing and ball sports. One of the major reasons causing hand and finger deformity is due to ligament and tendon injury. The aim of this study was to investigate if the high-resolution 3T magnetic resonance imaging (MRI) can demonstrate the complex anatomy of the fingers and thumb, especially the tendons and ligaments, and provide the accurate diagnosis of clinically important fingers and thumbs deformity due to ligamentous and tendinous injuries during sport activities. Methods: Sixteen fresh un-embalmed cadaveric hands were harvested from eight cadavers. A total of 20 healthy volunteers' hands and 44 patients with fingers or thumb deformity due to sports-related injuries were included in this study. All subjects had MR examination with T1-weighted images and proton density-weighted imaging with fat suppression (PD FS) in axial, coronal, and sagittal plane, respectively. Subsequently, all 16 cadaveric hands were sliced into 2-mm thick slab with a band saw (six in coronal plane, six in sagittal plane, and four in axial plane). The correlation of anatomic sections and the MRI characteristics of tendons of fingers and the ulnar collateral ligament (UCL) at the metacarpal phalangeal joint (MCPJ) of thumb between 20 healthy volunteers and 44 patients (confirmed by surgery) were analyzed. Results: The normal ligaments and tendons in 16 cadaveric hands and 20 volunteers' hands showed uniform low-signal intensity on all the sequences of the MRI. Among 44 patients with tendinous and ligamentous injuries in the fingers or thumb, 12 cases with UCL injury at MCPJ of the thumb (Stener lesion = 8 and non-Stener lesion = 4), 6 cases with the central slip injury, 12 cases with terminal tendon injury, and 14 cases with flexor digitorum profundus injury. The ligaments and tendons disruption manifested as increased signal intensity and poor definition, discontinuity, and heterogeneous signal intensity of the involved ligaments and tendons. Conclusions: Sports injury-related fingers and thumb deformity are relatively common. MRI is an accurate method for evaluation of the anatomy and pathologic conditions of the fingers and thumb. It is a useful tool for accurate diagnosis of the sports-related ligaments and tendons injuries in hand.
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- 2018
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10. Retinal arteriosclerosis in type 2 diabetes mellitus and its relationship with cardiovascular and cerebrovascular diseases
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Qin-Ying Ye, Xiao-Yan Huang, Chun-Yan Li, Xiao-Dong Liang, Wen-Ting Li, Zhen-Pei Zeng, Jun-Jie Chen, and Yu-Yin Huang
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type 2 diabetes mellitus ,fundus disease ,cardiocerebral vascular system ,Ophthalmology ,RE1-994 - Abstract
AIM: To investigate the relationship between retinal arteriosclerosisand cardiovascular and cerebrovascular diseases in type 2 diabetes mellitus. METHODS:Totally 85 patients with type 2 diabetes mellitus in our hospital from January 2016 to January 2017 were selected as the research subjects. Fundus examination was carried out to determine the classification of ophthalmic arteriosclerosis. The blood glucose level of the patients was detected and the glycosylated hemoglobin,fasting blood glucose and postprandial blood glucose levels were measured. All patients were followed up for at least 1 and a half years, and the cardiovascular and cerebrovascular diseases rate was statistically analyzed during the follow up period. The blood glucose level and cardiovascular and cerebrovascular diseases rate in patients with different ocular fundus arteriosclerosis grades were compared, meanwhile the relationship between ocular fundus arteriosclerosis grades with the blood glucose levels and cardiovascular and cerebrovascular diseases rate were analyzed. RESULTS: Among the 85 patients, 15%(13/85), 52%(44/85)and 33%(28/85)of the patients were classified asocular fundus arteriosclerosis grades Ⅰ, Ⅱ and Ⅲ respectively. The cardiovascular and cerebrovascular diseases rate during the follow up period was 27%(23/85). The glycosylated hemoglobin,fasting blood glucose, postprandial blood glucose and cardiovascular and cerebrovascular diseases rates were all increased with the elevation of ophthalmic arteriosclerosis(Prs=0.875, 0.792, 0.835, 0.878; PCONCLUSION:Type 2 diabetes mellitus patients has severe fundus arteriosclerosis, which is closely related to blood glucose level and cardiovascular and cerebrovascular diseases.
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- 2019
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11. Prolonged viral shedding in feces of children with COVID-19: a systematic review and synthesis of data
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Wen-Ting, Li, Yun, Zhang, Miao, Liu, Yan-Qin, Liu, and Xiang, Ma
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Adult ,Feces ,SARS-CoV-2 ,Pediatrics, Perinatology and Child Health ,Humans ,COVID-19 ,RNA, Viral ,Angiotensin-Converting Enzyme 2 ,Child ,Asymptomatic Infections ,Virus Shedding - Abstract
During the coronavirus disease 2019 (COVID-19) epidemic, many reports have indicated that children shed the virus longer than adults in stool, and that most of the children had mild or even asymptomatic infections, which increased the potential risk for feces to be a source of contamination and may play an important role in the spread of the virus. In this review, we collected relevant literature to summarize the duration of fecal viral shedding in children with COVID-19. We found that in about 60% of the cases, the fecal shedding time was between 28 and 42 days, which was much longer than that of adults. We further explored the possible reason for prolonged shedding and its the potential impact. The poor hand hygiene practices of children, their tendency to swallow sputum and/or saliva, the significant difference in expression of angiotensin-converting enzyme 2 (ACE2) in intestine between children and adults, and the variance in immune status and intestinal microbiome could be considered as potential casual agents of longer fecal viral shedding duration of children. Conclusion: Children with COVID-19 show prolonged fecal shedding compared to adults. Several mechanisms may be involved in the longer fecal viral shedding. Viral shedding in the stool could be contributing to a possible route of transmission. Therefore, we think that further preventive measures in children should be taken to reduce the spread of the disease. What is Known: • Children with COVID-19 are more likely to have asymptomatic infections and to experience mild symptoms. • Some patients continue to shed the virus in feces, despite respiratory samples testing negative. What is New: • Children with COVID-19 carried a longer-term fecal viral shedding than adults. • The poor hand hygiene practices of children, their tendency to swallow sputum and/or saliva, the difference in expression of ACE2 in intestine between children and adults, and the variance in immune status and intestinal microbiome could be considered as potential casual agents of longer fecal viral shedding duration of children.
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- 2022
12. Topic-Independent Chinese Sentiment Identification from Online News.
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Zhong-Yong Chen and Wen-Ting Li
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- 2017
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13. High-resolution 3T Magnetic Resonance Imaging of the Triangular Fibrocartilage Complex in Chinese Wrists: Correlation with Cross-sectional Anatomy
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Hui-Li Zhan, Wen-Ting Li, Rong-Jie Bai, Nai-Li Wang, Zhan-Hua Qian, Wei Ye, and Yu-Ming Yin
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Anatomy ,Magnetic Resonance Imaging ,Triangular Fibrocartilage Complex ,Wrist ,Medicine - Abstract
Background: The injury of the triangular fibrocartilage complex (TFCC) is a common cause of ulnar-sided wrist pain. The aim of this study was to investigate if the high-resolution 3T magnetic resonance imaging (MRI) could demonstrate the detailed complex anatomy of TFCC in Chinese. Methods: Fourteen Chinese cadaveric wrists (from four men and three women; age range at death from 30 to 60 years; mean age at 46 years) and forty healthy Chinese wrists (from 20 healthy volunteers, male/female: 10/10; age range from 21 to 53 years with a mean age of 32 years) in Beijing Jishuitan Hospital from March 2014 to March 2016 were included in this study. All cadavers and volunteers had magnetic resonance (MR) examination of the wrist with coronal T1-weighted and proton density-weighted imaging with fat suppression in three planes, respectively. MR arthrography (MRAr) was performed on one of the cadaveric wrists. Subsequently, all 14 cadaveric wrists were sliced into 2 mm thick slab with band saw (six in coronal plane, four in sagittal plane, and four in axial plane). The MRI features of normal TFCC were analyzed in these specimens and forty healthy wrists. Results: Triangular fibrocartilage, the ulnar collateral ligament, and the meniscal homolog could be best observed on images in coronal plane. The palmar and dorsal radioulnar ligaments were best evaluated in transverse plane. The ulnotriquetral and ulnolunate ligaments were best visualized in sagittal plane. The latter two structures and the volar and dorsal capsules were better demonstrated on MRAr. Conclusion: High-resolution 3T MRI is capable to show the detailed complex anatomy of the TFCC and can provide valuable information for the clinical diagnosis in Chinese.
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- 2017
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14. LncRNA IMFNCR Promotes Intramuscular Adipocyte Differentiation by Sponging miR-128-3p and miR-27b-3p
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Meng Zhang, Fang Li, Jun-wei Sun, Dong-hua Li, Wen-ting Li, Rui-rui Jiang, Zhuan-jian Li, Xiao-jun Liu, Rui-li Han, Guo-xi Li, Yan-bin Wang, Ya-dong Tian, Xiang-tao Kang, and Gui-rong Sun
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long non-coding RNA ,ceRNA ,miRNA ,intramuscular adipocyte differentiation ,chicken ,Genetics ,QH426-470 - Abstract
Poultry meat quality is affected by many factors, among which intramuscular fat (IMF) is predominant. IMF content affects the tenderness, juiciness, and flavor of chicken. An increasing number of studies are focusing on the functions of lncRNAs in adipocyte differentiation. However, little is known about lncRNAs associated with intramuscular adipocyte differentiation. In the present study, we focused on an up-regulated lncRNA during intramuscular adipogenetic differentiation, which we named intramuscular fat-associated long non-coding RNA (IMFNCR). IMFNCR promotes intramuscular adipocyte differentiation. In-depth analyses showed that IMFNCR acts as a molecular sponge for miR-128-3p and miR-27b-3p and that PPARG is a direct target of miR-128-3p and miR-27b-3p in chicken. High-fat and high-protein diet inhibited chicken IMFNCR level in vivo. Moreover, IMFNCR level was positively correlated with PPARG mRNA level in chicken breast muscle tissues, a vital corollary to ceRNA function. Altogether, our research showed that IMFNCR acts as a ceRNA to sequester miR-128-3p and miR-27b-3p, leading to heightened PPARG expression, and thus promotes intramuscular adipocyte differentiation. Taken together, our findings may contribute to a more thorough understanding of chicken IMF deposition and the improvement of poultry meat quality.
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- 2019
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15. Ibrutinib facilitates the sensitivity of colorectal cancer cells to ferroptosis through BTK/NRF2 pathway
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Jin-Feng Zhu, Yi Liu, Wen-Ting Li, Ming-Hui Li, Chao-Hui Zhen, Pei-Wei Sun, Ji-Xin Chen, Wen-Hao Wu, and Wei Zeng
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Cancer Research ,Cellular and Molecular Neuroscience ,Immunology ,Cell Biology - Abstract
Ibrutinib is a drug that inhibits the protein Burton’s tyrosine kinase and thereby the nuclear translocation of Nrf2, which played a key role in mediating the activation of antioxidants during stress conditions and ferroptosis resistance. This study aimed to identify the effect of Ibrutinib and ferroptosis inducer on colorectal cancer (CRC) treatment and its underlying mechanism. In our study, we found the upregulation of Nrf2 was correlated with CRC progression and antioxidant proteins. Ibrutinib sensitized CRC to ferroptosis inducers, suggested by further reduced CRC cell viability, proliferation and decreased antioxidant protein levels in CRC cells after combination treatment of Ibrutinib and RSL3 or Ibrutinib and Erastin both in vivo and in vitro. Knockout of Nrf2 diminished the regulatory effect of Ibrutinib on CRC sensitivity to ferroptosis inducers. Altogether, this study demonstrated that Ibrutinib increases the sensitivity of CRC cell to ferroptosis inducers by inhibiting Nrf2.
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- 2023
16. Does novel oral anticoagulant improve anticoagulation for non-valvular atrial fibrillation associated stroke: An inpatient registration study in Shanghai
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Feng-Di Liu, Rong Zhao, Xue-Mei Wang, Shuo Wang, Xiao-Lei Shen, Xiao-Xiao Tao, Bo Zheng, Jia-Li Peng, Hui Zhang, Ran Mo, Yan Tong, Wen-Ting Li, Xiao-Yan Feng, Ge-Fei Li, Liang Shu, and Jian-Ren Liu
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Medicine (General) ,R5-920 - Abstract
Abstracts: Objective: To summarize the use rate, safety, efficacy of antithrombotics in stroke/transient ischemic attack (TIA) prevention, and reasons for not using dabigatran etexilate (DE) in Shanghai, China. Methods: Non-valvular atrial fibrillation (NVAF)-associated stroke patients were prospectively registered as an electronic database. Use rate of antithrombotics and reasons for not using DE were extracted during follow-up. Patients' baseline characteristics, recurrent ischemic stroke/TIA events and bleeding complications were analyzed. Patients: From April 2012 to August 2014, 110 inpatients with NVAF-associated stroke were studied in our hospital. NVAF was diagnosed by 12-lead electrocardiogram, 24 h Holter and echocardiography. Results: Before introduction of DE (April 2013), use rates of warfarin and antiplatelets were 28.9% (11/38) and 60.5% (23/38) respectively; after that, use rates of warfarin, DE, and antiplatelets were 20.8% (15/72), 12.5% (9/72), and 43.1% (31/72). The DE did not improve use of anticoagulants (P = 0.639). There were 19 (17.3%) recurrent ischemic stroke events up to October 2015; two (9.5%) in the non-user group, 10 (18.5%) in the antiplatelet group, and seven (20.0%) in the anticoagulants group (P = 0.570). Furthermore, recurrence rates were similar between the DE group (20.0%) and the Warfarin group (20.0%, P = 1.000). The most common reason for not using DE was financial concerns (61.0%), followed by inconvenience to purchase (14.0%) and hemorrhage concerns (11.0%). Two patients using warfarin found fecal occult blood so they stopped warfarin and began to use antiplatelet drugs. No bleeding event occurred in the other groups. Only one patient had side effects (dyspepsia and gastroesophageal reflux) from DE. Conclusion: The use rate of either DE or warfarin in Shanghai was low; DE had not improved anticoagulation therapy for NVAF patients in Shanghai mainly because DE had not been covered by health insurance. Keywords: Dabigatran etexilate, Novel oral anticoagulant, Cardiogenic cerebral embolism, Non-valvular atrial fibrillation, Real-world study
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- 2015
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17. Identification of the Key microRNAs and miRNA-mRNA Interaction Networks during the Ovarian Development of Hens
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Jing Li, Chong Li, Qi Li, Wen-Ting Li, Hong Li, Guo-Xi Li, Xiang-Tao Kang, Xiao-Jun Liu, and Ya-Dong Tian
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DE miRNAs ,ovarian function ,target genes ,different stages ,hens ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
It is well-known that multiple functional miRNAs are found in mammals’ ovaries, which are linked not only to ovarian development, but also to maturation and apoptosis. However, there is still a lack of knowledge regarding the role of miRNAs in the hen ovary. In the present study, we analyzed the miRNA sequencing libraries of ovaries at the four different developmental stages of hens (15, 20, 30, and 68 W) and a total of 677 known miRNAs and 61 novel miRNAs were identified. In total, 209 of them were differently expressed miRNAs (DE miRNAs) obtained from comparisons of the four stages, including 84 upregulated and 125 downregulated DE miRNAs. Furthermore, the five key DE miRNAs gga-miR-2954, gga-miR-6634-5p, gga-miR-449b-5p, gga-miR-449c-3p, and gga-miR449c-5p were screened using an analysis of the miRNA-mRNA interaction network and functional enrichment annotated in seven significantly enriched pathways, such as endocytosis, lysine degradation, the biosynthesis of amino acids, and the MAPK signaling pathway, which may primarily participate in cell differentiation and proliferation, steroid hormone biosynthesis, and angiogenesis by targeting the related genes. For instance, gga-miR-449 family members were predicted to target 15 genes, including TGFB1, TPM1, TPM3, and CAMKB2, which were reported to regulate follicular growth, selection, and the ovulatory cycle. Taken together, our results illustrate the ovarian miRNA profiles of the four classic developmental stages of hens and highlight the significant role of miRNAs in ovarian development and functions. However, in-depth research needs to be carried out to validate the potential functional miRNAs found in this study.
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- 2020
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18. Olanzapine Induces Inflammation and Immune Response via Activating ER Stress in the Rat Prefrontal Cortex
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Wen ting Li, Xu-Feng Huang, Meng He, Tao lei Sun, Bao hua Zhang, Chao Deng, and Kun Qian
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CD14 ,Interleukin-1beta ,Nitric Oxide Synthase Type II ,Prefrontal Cortex ,Apoptosis ,Inflammation ,Pharmacology ,Biochemistry ,Pathogenesis ,Immune system ,Genetics ,Animals ,Humans ,Medicine ,Receptor ,Interleukin-6 ,business.industry ,Immunity ,Endoplasmic Reticulum Stress ,Toll-Like Receptor 2 ,I-kappa B Kinase ,Rats ,Disease Models, Animal ,Oxidative Stress ,TLR2 ,Gene Expression Regulation ,Olanzapine ,Schizophrenia ,TLR4 ,Unfolded protein response ,medicine.symptom ,business ,Signal Transduction - Abstract
Objective Antipsychotics, in particular olanzapine, are first-line medications for schizophrenia. The prefrontal cortex (PFC) is an important region for antipsychotics' therapeutic effects. The PFC inflammatory and immune pathways are associated with schizophrenia pathogenesis. However, the effect of antipsychotics on the inflammatory and immune pathways in the PFC remains unclear. We aimed to examined the time-dependent effect of olanzapine on inflammatory and immune markers in the PFC of rats. Since the inflammatory and immune pathways are related to endoplasmic reticulum (ER) stress, we further investigated whether or not olanzapine-induced inflammation and immune responses were related to ER stress. Methods Expression of pro-inflammatory markers including IkappaB kinase β (IKKβ), nuclear factor kappa B (NFκB), tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) and IL-1β, and immune-related proteins including inducible nitric oxide synthase (iNOS), toll-like receptor 2 (TLR2) and cluster of differentiation 14 (CD14) were examined by Western blotting. Results Olanzapine treatments for 1, 8 and 36 days significantly activated the inflammatory IKKβ/NFκB signaling, and increased the expression of TNF-α, IL-6, IL-1β and immune-related proteins such as iNOS, TLR4 and CD14. Olanzapine treatment for 1 day, 8 and 36 days also induced ER stress in the PFC. Co-treatment with an ER stress inhibitor, 4-phenylbutyrate, inhibited olanzapine-induced inflammation and the immune response in the PFC. Conclusion These results suggested olanzapine exposure could be a factor that induces central inflammation and immunological abnormities in schizophrenia subjects. Olanzapine induces PFC inflammation and immune response, possibly via activating ER stress signaling.
- Published
- 2021
19. Two new monoterpene esters from
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Meng-Yuan, Jiang, Xiao-Yun, Pu, Wen-Ting, Li, Juan, Liu, Xiao-Li, Zeng, Hong-Rui, Li, Xi-Shan, Bai, Lin, Hu, and Xiang-Zhong, Huang
- Abstract
Two new monoterpene esters, illigerates H and I (
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- 2022
20. Electroacupuncture on Hemifacial Spasm and Temporomandibular Joint Pain Co-Morbidity: A Case Report
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Jian-peng Huang, Zhan-mou Liang, Qi-wen Zou, Jie Zhan, Wen-ting Li, Sheng Li, Kai Li, Wen-bin Fu, and Jian-hua Liu
- Subjects
Neurology ,Neurology (clinical) - Abstract
Hemifacial spasm (HFS) and temporomandibular joint (TMJ) pain are common facial diseases which cause depression, anxiety, insomnia, and poor quality of life. However, currently there are still no effective therapies to treat HFS and TMJ. Electroacupuncture (EA) has advantages of safety, rapid work, easy operation and convenience. Here, we reported a case of a 50-year-old woman who presented with irregular spasm of eyelids and facial muscles on the left side, and TMJ pain on the right side. The patient had been treated with carbamazepine (20mg per day) and alternative therapies for a year, but still not much improvement in the symptoms. The scores of the Jankovic Rating Scale (JRS), global rating scale (GRS), and visual analog scale (VAS) were 7, 60, and 7 points, respectively. The EMG test showed that the spastic side had higher R1 amplitude, longer R2 duration, and larger R2 area than the non-spasmodic side, and the occurrence rate of the lateral spread responses (LSR) in the Orbicularis oris and the Orbicularis oculi muscle was 60% and 40%, respectively. We considered this patient had left HFS and right TMJ pain. EA was successfully undertaken for two periods over 30 weeks. After EA, JRS and VAS were reduced sharply, and the symptoms of HFS were stable without recurrence. However, the frequency of the lower eyelid increased gradually during the 6-month follow-up. These findings reveal that EA with the frequency of 2 Hz and intensity of ~ 1–2 mA may be a benefit for alleviating symptoms of HFS and TMJ pain without adverse reaction. The potential mechanisms of EA in HFS and TMJ pain co-morbidity involve brain stem mechanism and DNIC mechanism for distal acupuncture and segmental mechanism for local acupuncture analgesia.
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- 2022
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21. Corilagin Attenuates Aerosol Bleomycin-Induced Experimental Lung Injury
- Author
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Zheng Wang, Qiong-Ya Guo, Xiao-Ju Zhang, Xiao Li, Wen-Ting Li, Xi-Tao Ma, and Li-Jun Ma
- Subjects
pulmonary fibrosis ,corilagin ,NF-κB ,apoptosis ,TGF-β1 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressing lethal disease with few clinically effective therapies. Corilagin is a tannin derivative which shows anti-inflammatory and antifibrotics properties and is potentiated in treating IPF. Here, we investigated the effect of corilagin on lung injury following bleomycin exposure in an animal model of pulmonary fibrosis. Corilagin abrogated bleomycin-induced lung fibrosis as assessed by H&E; Masson’s trichrome staining and lung hydroxyproline content in lung tissue. Corilagin reduced the number of apoptotic lung cells and prevented lung epithelial cells from membrane breakdown, effluence of lamellar bodies and thickening of the respiratory membrane. Bleomycin exposure induced expression of MDA, IKKα, phosphorylated IKKα (p-IKKα), NF-κB P65, TNF-α and IL-1β, and reduced I-κB expression in mice lung tissue or in BALF. These changes were reversed by high-dose corilagin (100 mg/kg i.p) more dramatically than by low dose (10 mg/kg i.p). Last, corilagin inhibits TGF-β1 production and α-SMA expression in lung tissue samples. Taken together, these findings confirmed that corilagin attenuates bleomycin-induced epithelial injury and fibrosis via inactivation of oxidative stress, proinflammatory cytokine release and NF-κB and TGF-β1 signaling. Corilagin may serve as a promising therapeutic agent for pulmonary fibrosis.
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- 2014
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22. Influence of aboveground structure on connected underground structure seismic response within an integrated system
- Author
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Wen-Ting Li, Rui Wang, and Jian-Min Zhang
- Subjects
Building and Construction ,Geotechnical Engineering and Engineering Geology - Published
- 2023
23. Biological Mechanism of Specific Relationship between Gan (Liver) Meridian of Foot-Jueyin and Genitals
- Author
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Jianhua Liu, Nenggui Xu, Yu Zhu, Wen-Ting Li, Jianpeng Huang, and Jia-Ming Zhong
- Subjects
Cortical homunculus ,business.industry ,Medial side ,0211 other engineering and technologies ,02 engineering and technology ,General Medicine ,Anatomy ,Thigh ,030226 pharmacology & pharmacy ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Complementary and alternative medicine ,Cerebral cortex ,Cortex (anatomy) ,External genitalia ,021105 building & construction ,Medicine ,Pharmacology (medical) ,Meridian (astronomy) ,business ,Foot (unit) - Abstract
From the perspective view of Chinese medicine, the Gan (Liver) meridian of Foot-Jueyin starts from the great toe, running upward along the medial side of the thigh to the perineal area, where it curves around the external genitalia and goes up to the lower abdomen. In clinical practice, acupoints in the feet of the Gan meridian of Foot-Jueyin are used to treat the genitourinary and external genitalia diseases. Studies have shown that reproductive system diseases have specific pathological reactions in the places (radial side of tibia and foot) where Gan meridian of Foot-Jueyin passes by. Why does this happen? In this article, we begin by briefly reviewing the evidences linking foot and genitalia. We then explore the potential mechanism of the relationship between genitals and the Gan meridian of Foot-Jueyin. The brain cerebral cortex is characterized by cortical interactions. Numerous studies show that different cerebral cortex function areas (especially the adjacent areas) are overlapping and interact with each other. Finally, we presume that there is a specific connection between the feet and the genitals. Physiologically in the cortical homunculus, the genital area lies adjacent or overlapped to the foot areas, the two areas may interact with each other. The functional reorganization between different areas of the cerebral cortex under pathological conditions may be the underlying mechanism of the relationship between the feet and the genitals.
- Published
- 2021
24. CHOP/ORP150 Ratio in Endoplasmic Reticulum Stress: A New Mechanism for Diabetic Peripheral Neuropathy
- Author
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Yuan-Bo Wu, Hong-Qi Li, Ming-Shan Ren, Wen-Ting Li, Xin-Yi Lv, and Li Wang
- Subjects
Reactive oxygen species (ROS) ,Apoptosis ,Schwann cells (SCs) ,ER stress ,CHOP ,Diabetic peripheral neuropathy (DPN) ,ORP150 ,Type II diabetes mellitus ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Peripheral neuropathy is a frequent and severe diabetic complication characterized by progressive loss of peripheral nerve axons and manifested by pain and eventually complete loss of sensation. Effective therapy for diabetic peripheral neuropathy (DPN) is still lacking due to our limited understanding of the mechanisms for nerve injury. Methods: Here we tested the roles of endoplasmic reticulum (ER) stress and the ER stress-activated pro-apoptotic protein CHOP and anti-apoptotic protein ORP150 in DPN in a rat model of high-fat/streptozotocin diabetes and in cultured Schwann cells (SCs). Results: No significant DPN was seen in the early stage of diabetes (4 weeks following verification of diabetes). However, after prolonged diabetes (16 weeks following verification of diabetes), DPN was severely developed as reflected by slowed motor and sensory nerve conduction velocity, blunted thermal nociception, and decreased intraepidermal nerve fiber profiles in the hindpaw. Meanwhile, while it was not noticed in sciatic nerves of early diabetes, ER stress in prolonged diabetic rats was indicated by robust increases in H2O2 production and expression of the ER chaperon glucose-regulated protein 78 (GRP78). ORP150 expression was substantially upregulated, accompanied by mild increase in CHOP expression, resulting in a low CHOP/ORP150 ratio, in early diabetes. In contrast, with prolonged diabetes, CHOP expression exceeded ORP150 expression, resulting in an increased CHOP/ORP150 ratio. In vivo knockdown of ORP150 induced DPN in early diabetes and exacerbated the DPN after prolonged diabetes, whereas knockdown of CHOP ameliorated DPN in rats with prolonged diabetic. On the other hand, in vitro knockdown of ORP150 promoted high glucose-induced SC apoptosis, whereas knockdown of CHOP protected SCs from apoptosis. Conclusion: Taken together, we have provided evidence for the critical role of ER stress in the development of DN and also uncovered CHOP/ORP150 ratio as an important mechanism for determining neuronal apoptosis during ER stress. In the early stage of diabetes, CHOP/ORP150 ratio was relatively low favoring neuronal cell survival, whereas after prolonged diabetes, CHOP/ORP150 ratio increased resulting in apoptotic cell death leading to accelerated DPN.
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- 2013
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25. The Association between Helicobacter pylori Infection and Chronic Hepatitis C: A Meta-Analysis and Trial Sequential Analysis
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Juan Wang, Wen-Ting Li, Yi-Xiang Zheng, Shu-Shan Zhao, Ning Li, Yan Huang, Rong-Rong Zhou, Ze-Bing Huang, and Xue-Gong Fan
- Subjects
Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Purpose. Helicobacter pylori is a common gastric disease-inducing pathogen. Although an increasing number of recent studies have shown that H. pylori is a risk factor for liver disease, the potential association between H. pylori infection and chronic hepatitis C still remains controversial. The aim of our meta-analysis was to evaluate a potential association between H. pylori infection and chronic hepatitis C. Methods. We searched the PubMed, Embase, CNKI, Web of Science, and the Cochrane Central Register of Controlled Trials (CENTRAL) databases between January 1, 1994, and May 1, 2015. Results. This study included a total of 1449 patients with chronic hepatitis C and 2377 control cases. The prevalence of H. pylori was significantly higher in patients with chronic hepatitis C than in those without chronic hepatitis C. The pooled odds ratio was 2.93. In a subgroup analysis, the odds ratios were 4.48 for hepatitis C virus- (HCV-) related cirrhosis and 5.45 for hepatocellular carcinoma. Conclusion. Our study found a strong association between H. pylori and chronic hepatitis C, particularly during the HCV progression stage; thus, we recommend active screening for H. pylori in patients with chronic hepatitis C.
- Published
- 2016
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26. CircFNDC3B sequestrates miR‐937‐5p to derepress TIMP3 and inhibit colorectal cancer progression
- Author
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Jin-Feng Zhu, Wen-Ting Li, Yi Liu, Yi Li, and Wei Zeng
- Subjects
Male ,0301 basic medicine ,Cancer Research ,TIMP3 ,Angiogenesis ,Mice, Nude ,colorectal cancer ,Biology ,lcsh:RC254-282 ,Metastasis ,angiogenesis ,03 medical and health sciences ,Exon ,0302 clinical medicine ,Circular RNA ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Animals ,Humans ,neoplasms ,Research Articles ,Tissue Inhibitor of Metalloproteinase-3 ,Mice, Inbred BALB C ,Gene knockdown ,RNA ,RNA, Circular ,General Medicine ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,digestive system diseases ,Microvesicles ,Fibronectins ,Gene Expression Regulation, Neoplastic ,liver metastasis ,MicroRNAs ,030104 developmental biology ,miR‐937‐5p ,Oncology ,030220 oncology & carcinogenesis ,circFNDC3B ,Disease Progression ,Cancer research ,Molecular Medicine ,Female ,Colorectal Neoplasms ,Research Article - Abstract
CircFNDC3B induced TIMP3 to suppress angiogenesis to inhibit CRC cell proliferation, migration and invasion by negatively targeting miR‐937‐5p. Also, circFNDC3B exosomes could repress CRC angiogenesis by decreasing VEGFR expression., Circular RNA (circRNA) are single‐stranded RNA with covalently closed 3′ and 5′ ends, with many recognized to be involved in human diseases as gene regulators, typically by interacting with other RNA. CircFNDC3B is a circRNA formed by back‐splicing of exons 5 and 6 of the FNDC3B gene. CircFNDC3B was recently implicated in renal carcinoma, gastric and bladder cancer. However, the expression levels of circFNDC3B and its role in colorectal cancer (CRC) remain unclear. Expression of circFNDC3B and TIMP3 levels in CRC tissues and cell lines were found to be low, whereas microRNA (miR)‐937‐5p expression was high in CRC. MicroRNA‐937‐5p downregulated TIMP3, thereby promoting tumor cell proliferation, invasion, migration and angiogenesis. Moreover, CircFNDC3B was shown to bind to miR‐937‐5p. CircFNDC3B and circFNDC3B‐enriched exosomes inhibited tumorigenic, metastatic and angiogenic properties of CRC, and miR‐937‐5p overexpression or TIMP3 knockdown could reverse these effects. In vivo CRC tumor growth, angiogenesis and liver metastasis were suppressed by circFNDC3B overexpression, circFNDC3B‐enriched exosomes or miR‐937‐5p knockdown. In conclusion, our work reports a tumor‐suppressing role for the circFNDC3B–miR‐97‐5p–TIMP3 pathway and suggests that circFNDC3B‐enriched exosomes can inhibit angiogenesis and CRC progression.
- Published
- 2020
27. Fluorescent Sensor Array for Tetracyclines Discrimination Using a Single Dye@Mof Composite Sensor
- Author
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Wen-Ting Li, Jia-Si Wang, Meili Pang, Yue Li, and Wen-Juan Ruan
- Subjects
History ,Polymers and Plastics ,Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Business and International Management ,Condensed Matter Physics ,Instrumentation ,Industrial and Manufacturing Engineering ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
28. Association of changes in NT-proBNP, hsTnT and uric acid levels with haemodynamic changes after targeted medical therapies in patients with idiopathic pulmonary arterial hypertension
- Author
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Chang-Wei Wu, Jin-Ming Liu, and Wen-Ting Li
- Subjects
Adult ,Male ,medicine.medical_specialty ,medicine.drug_class ,Hypertension, Pulmonary ,Cardiac index ,Hemodynamics ,Renal function ,chemistry.chemical_compound ,Internal medicine ,Natriuretic Peptide, Brain ,medicine ,Natriuretic peptide ,Humans ,Familial Primary Pulmonary Hypertension ,business.industry ,Cardiovascular Topics ,Idiopathic Pulmonary Arterial Hypertension ,General Medicine ,Middle Aged ,Peptide Fragments ,Uric Acid ,medicine.anatomical_structure ,chemistry ,Vascular resistance ,Cardiology ,Uric acid ,Biomarker (medicine) ,Female ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers - Abstract
BACKGROUND: During pulmonary arterial hypertension (PAH)- targeted therapies for patients with idiopathic pulmonary arterial hypertension (IPAH), regular follow up to evaluate treatment efficacy is essential. Serum biomarkers can reflect various pathobiological processes in IPAH and have the advantages of being non-invasive, simple to carry out and low cost. The aim of our study was to evaluate whether serum biomarkers could serve as non-invasive markers to reflect haemodynamic changes after PAH-targeted therapies in patients with IPAH. METHODS: A total of 31 eligible patients aged 38.1 ± 12.1 years (25 were female) were included in this study. Changes in haemodynamic parameters and several serum biomarkers (cardiac markers, serum uric acid, high-sensitivity C-reactive protein, hepatic and kidney function markers) were compared before and after at least six months of PAH-targeted therapies. The time interval between the blood assays and right heart catheterisation was within five days. RESULTS: After at least six months of PAH-targeted therapies, the N-terminal pro-brain natriuretic peptide (NT-proBNP) level decreased from 579 (191–905) to 135 pg/ml (60–395) (p < 0.01), high-sensitivity cardiac troponin T (hsTnT) level decreased from 0.009 (0.006–0.012) to 0.007 ng/ml (0.005– 0.01) (p < 0.01), and serum uric acid level decreased from 381.5 ± 131.4 to 327.2 ± 110.0 μmol/l (p = 0.011). The change in NT-proBNP level was positively correlated with changes in pulmonary vascular resistance (r = 0.538, p < 0.01) and mean pulmonary arterial pressure (r = 0.440, p = 0.013). The change in hsTnT level was positively correlated with the change in mean right atrium pressure (r = 0.504, p < 0.01). The change in serum uric acid level was negatively correlated with that of cardiac index (r = –0.471, p < 0.01). CONCLUSION: NT-proBNP, hsTnT and serum uric acid levels can be used as non-invasive tools for evaluating the efficacy of PAH-targeted medications for IPAH patients. The role of these biomarkers in the follow up should be emphasised.
- Published
- 2021
29. Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts
- Author
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Yi-hao Wang, Yi-jing Chu, Yan Li, Shan Feng, Ya-jun Liu, Hong-min Xi, Wen-ting Li, and Chen Wang
- Subjects
Cell ,Apoptosis ,Exosomes ,Biochemistry ,Extracellular matrix ,Tripartite Motif Proteins ,Cell Movement ,Pregnancy ,Genetics ,medicine ,Humans ,Molecular Biology ,reproductive and urinary physiology ,Cell Proliferation ,Migration Assay ,biology ,Chemistry ,Mesenchymal stem cell ,Ubiquitination ,Trophoblast ,Mesenchymal Stem Cells ,Microvesicles ,Ubiquitin ligase ,Cell biology ,Trophoblasts ,medicine.anatomical_structure ,MRNA Sequencing ,embryonic structures ,biology.protein ,Female ,Stem cell ,Chorionic Villi ,Tumor Suppressor Protein p53 ,Biotechnology - Abstract
Background: It was designed to investigate whether umbilical cord matrix stem cells (CVMSC) could affect the functionality of trophoblasts to prevent preeclampsia. Methods: In this study, CVMSC-derived exosomes were isolated; its effect on trophoblasts was investigated based on the CCK8 assay, migration assay, and apoptosis detection. And the underlying mechanism of this effect was investigated using mRNA sequencing, western blot, co-immunoprecipitation, luciferase report assay, and ubiquitination assay. Findings: The results showed that CVMSC-derived exosomes promoted migration and proliferation of trophoblasts, and also reduced cell apoptosis. mRNA sequencing confirmed that after treatment of CVMSC-derived exosomes, TRIM72 expression was upregulated and P53 expression was downregulated, both significantly in trophoblasts. Subsequent study confirmed that TRM72 could directly interact with P53 and promoted P53 ubiquitination and proteasomal degradation, reducing apoptosis rate and elevating proliferation and migration in trophoblasts. Interpretation: Our study confirmed that CVMSC-derived exosomes promoted trophoblast migration and proliferation by upregulating TRIM72 expression, and subsequently advanced P53 ubiquitination and proteasomal degradation. Funding Statement: This study received no specific grant from public funding agencies, commercial, or not-for-profit sectors. Declaration of Interests: All authors declare there is no competing interest. Ethics Approval Statement: This study was approved by the Ethics Committee of the Affiliated Hospital of Qingdao University.
- Published
- 2021
30. Effects of a Group of High-Rise Structures on Ground Motions under Seismic Excitation
- Author
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Qing-jun Chen and Wen-ting Li
- Subjects
Physics ,QC1-999 - Abstract
A three-dimensional simulation was created to determine the seismic performance of coupled systems with a group of up to 100 pile-high-rise structures resting on soil layers using system modal, harmonic, and time domain analysis. The results demonstrated that the existence of a structural group mitigates the structural responses with respect to the single-structure-soil interaction (SSI) and results in significantly nonuniform ground seismic motions. Due to the influence of a structural group, adjacent structures can exhibit fully alternating mechanical behavior, and buildings in the urban fringe are subjected to stronger shaking than downtown buildings. The overall trend of the influence of structural groups is that ground motions are lessened inside an urban area, and ground motions at the locations between structures differ from those at the locations of the structures. Moreover, the effective distance of a structural group on ground motions is associated with the urban width. Less distance between structures enhances the interaction effect. In addition, the soil properties can greatly influence the system’s seismic responses and can even completely change the effect trends. The results in our paper are consistent with the phenomena observed in the Mexico City earthquake and the 1976 earthquake in Friuli, Italy.
- Published
- 2015
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31. Multi-dimensional ratiometric fluorescent sensing of organic disinfectants using a MOF composite with independent responsive components
- Author
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Qi Yang, Feng-Yang Xie, Jia-Si Wang, Hong-Yi Yu, Wen-Ting Li, Yu-Hao He, Meili Pang, Yue Li, and Wen-Juan Ruan
- Subjects
Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
32. STX2 Promotes Trophoblast Growth, Migration, and Invasion Through Activation of the PI3K-AKT Pathway in Preeclampsia
- Author
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Can Li, Chun-ling Ma, Xian-li Sun, Yan Li, Yi-hao Wang, Chao Li, Ying Zhan, and Wen-ting Li
- Subjects
0301 basic medicine ,QH301-705.5 ,animal diseases ,Protein subunit ,Biology ,Immunofluorescence ,PI3K ,syntaxin2 ,preeclampsia ,Cell and Developmental Biology ,03 medical and health sciences ,chemistry.chemical_compound ,fluids and secretions ,0302 clinical medicine ,Mediator ,hemic and lymphatic diseases ,medicine ,LY294002 ,Biology (General) ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Original Research ,medicine.diagnostic_test ,Akt/PKB signaling pathway ,AKT ,Trophoblast ,Cell Biology ,bacterial infections and mycoses ,trophoblast ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,bacteria ,Developmental Biology - Abstract
ObjectivesAbnormal trophoblast behaviors during pregnancy contribute to the development of preeclampsia (PE). Syntaxin2 (STX2) has been shown to be a crucial epithelial mediator in numerous diseases. However, the functions of STX2 and the mechanisms underlying its role in PE remain largely unknown. The aim of this study was to explore the role of STX2 on trophoblast biology and unravel the molecular mechanisms that contribute to the development and progression of PE.Materials and MethodsWe first compared the expression of STX2 in placental tissues from women with PE and women with normal pregnancies. Then, we investigated the role of STX2 on trophoblast proliferation, migration and invasion in HTR-8/SVneo and primary human trophoblast cells by loss or gain of function experiments. In addition, co-immunoprecipitation, pulldown and immunofluorescence assays were performed to investigate the co-localization of STX2 with other proteins, and to help clarify the mechanisms underlying STX2-mediated functions on trophoblasts.ResultsWe demonstrated that STX2 expression was downregulated in placental tissues of women with PE compared with those from normal pregnancies. Loss and gain of function experiments further confirmed a role for STX2 in cell proliferation, migration and invasion in trophoblasts. By co-immunoprecipitation, pulldown and immunofluorescence co-localization assays, we revealed that STX2 selectively interacted with p85, a subunit of PI3K, and directly recruited p85 to the cytomembrane, thereby activating the AKT signaling pathway. We further demonstrated that the AKT activation was abolished by the use of a PI3K inhibitor (LY294002), which negatively affected STX2-mediated functions on trophoblasts.ConclusionAll together, our findings point to a crucial role for STX2 in PE progression. Our new insights also suggest that STX2 may be a potential diagnostic tool and a novel therapeutic target for treating PE.
- Published
- 2021
33. High-resolution 3T magnetic resonance imaging and histological analysis of capsuloligamentous complex of the first metatarsophalangeal joint
- Author
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H L Zhan, Z H Qian, Jin-e Wang, Wen-Ting Li, Yuming Yin, Rong-jie Bai, and Nai-Li Wang
- Subjects
Male ,Metatarsophalangeal Joint ,medicine.medical_specialty ,Histological analysis ,Diseases of the musculoskeletal system ,Masson's trichrome stain ,Finger Phalanges ,Capsuloligamentous complex ,medicine ,Cadaver ,Humans ,Orthopedics and Sports Medicine ,Plantar plate ,Orthopedic surgery ,Ligaments ,medicine.diagnostic_test ,business.industry ,Foot ,Magnetic resonance imaging ,Anatomy ,Magnetic Resonance Imaging ,First metatarsophalangeal joint ,Sagittal plane ,medicine.anatomical_structure ,RC925-935 ,Coronal plane ,Ligament ,Surgery ,Female ,Cadaveric spasm ,business ,RD701-811 ,Research Article - Abstract
Background There are discrepancies in the understanding of the structure of the capsuloligamentous complex of the first metatarsophalangeal joint (MTPJ); this study aims to investigate the differences with previous anatomical reports of high-resolution 3T magnetic resonance imaging (MRI) and histological analysis in illustrating the structure of the capsuloligamentous complex of the first MTPJ. Methods Nine fresh frozen cadaveric feet specimens (from two women and three men; aged 32 to 58 years) were used in this study. All specimens underwent MR examination with T1-weighted imaging and T2-weighted spectral attenuated inversion recovery in three planes. Subsequently, all cadaveric feet specimens were sliced into 2-mm-thick sections. The MRI features of the capsuloligamentous complex of the first MTPJ were analyzed in these specimens. Hematoxylin–eosin and Masson’s trichrome staining methods were used to explore the histologic features of the capsuloligamentous complex of the first MTPJ. Results Different from most previous studies, our results showed that the plantar plate could be divided into four portions including the central portion of the plantar plate, the intersesamoid, the sesamoid phalangeal and the metatarsosesamoid ligaments. The normal central portion of the plantar plate could be clearly visualized in the sagittal and coronal plane MR images. The intersesamoid ligament is a continuation of the central portion of the plantar plate on the sagittal plane on the gross specimen, the MR imaging, and the histological examination. On the coronal plane of the gross specimen and MR imaging, the sesamoid phalangeal ligaments and the central portion of the plantar plate can be seen as separate ligaments, but they appeared interwoven with the same continuous collagenous fibers on the histological analysis. Conclusion High-resolution 3T MRI allows accurate demonstration of the different anatomical details of the capsuloligamentous complex of the first MTPJ from previous anatomical reports. The histological analysis provides further understanding of the structures of the capsuloligamentous complex of the first MTPJ from previous studies.
- Published
- 2021
34. Electroacupuncture-induced plasticity between face and hand representations in motor cortex is associated with recovery of function after facial nerve injury
- Author
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Jianpeng Huang, Jianhua Liu, Hui Zhang, Yi-ling Yang, Dian Zeng, Li Jiang, and Wen-Ting Li
- Subjects
business.industry ,Electroacupuncture ,medicine.medical_treatment ,General Medicine ,Plasticity ,Facial nerve injury ,medicine.anatomical_structure ,Complementary and alternative medicine ,Acupuncture ,Medicine ,Neurology (clinical) ,business ,Neuroscience ,Motor cortex - Published
- 2020
35. Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China
- Author
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Changhui Chen, Qinghong Meng, Kaihu Yao, Suyun Qian, Qing Wang, Chen Sun, De-Nian Wen, Wen-Ting Li, Wei Shi, and Xin Yang
- Subjects
Male ,Methicillin-Resistant Staphylococcus aureus ,China ,Staphylococcus aureus ,Adolescent ,Genotype ,Erythromycin ,Microbial Sensitivity Tests ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,Minimum inhibitory concentration ,0302 clinical medicine ,030225 pediatrics ,Drug Resistance, Bacterial ,Humans ,Medicine ,030212 general & internal medicine ,Child ,Respiratory Tract Infections ,Respiratory tract infections ,business.industry ,SCCmec ,Infant ,Staphylococcal Infections ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Antimicrobial ,Anti-Bacterial Agents ,Bacterial Typing Techniques ,Penicillin ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Female ,Nasal Cavity ,business ,medicine.drug - Abstract
The molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus (S. aureus) in general pediatric wards and county-level hospitals were rarely reported in China. Staphylococcus aureus was isolated from children hospitalized with respiratory tract infection (RTI) in Zhongjiang and Youyang counties in 2015. All isolates were typed by multilocus sequence, staphylococcal protein A, accessory gene regulator (agr), and staphylococcal cassette chromosome mec [SCCmec, for methicillin-resistant S. aureus (MRSA) only]. Polymerase chain reaction was used to screen 21 super-antigen (SAg) genes and panton–valentine leukocidin (pvl). Antimicrobial susceptibility testing was performed by E test. A total of 2136 children were enrolled. Overall, 125 (5.9%) children carried S. aureus, among which MRSA accounted for 42.4%. ST59-SCCmec type IV-t437-agr group I (58.5%) was the most prevalent genotype in MRSA, and ST188-t189-agr group I (22.2%) was the top genotype in methicillin-sensitive S. aureus (MSSA). The pvl carriage rate in MRSA and MSSA was 15.1% and 9.7%, respectively (P = 0.4112). About 96.8% of S. aureus isolates were positive for at least one SAg gene. The most common SAg gene profile in the dominant ST59 clone was seb–sek–seq (42.8%). All S. aureus isolates were resistant to penicillin and erythromycin (minimum inhibitory concentration 90 was > 32 and 256 mg/L to penicillin and erythromycin, respectively), but usually susceptible to other tested non-β-lactam antimicrobials. Staphylococcus aureus and MRSA were detected with a high frequency in children with RTI in county-level hospitals of China. ST59-SCCmec type IV-t437-agr group I was the dominant MRSA clone. The S. aureus isolates exhibited high resistance to penicillin and erythromycin.
- Published
- 2019
36. Vagal Transient Receptor Potential Ankyrin 1 Mediates Stress-exacerbated Visceral Mechanonociception After Antral Cold Exposure
- Author
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Qing-Qing Luo, Xiu-Juan Yan, Sheng-Liang Chen, Xin Chen, and Wen-Ting Li
- Subjects
medicine.medical_specialty ,Abdominal pain ,Stress ,Vagus nerve ,03 medical and health sciences ,Transient receptor potential channel ,0302 clinical medicine ,Internal medicine ,medicine ,Ankyrin ,TRPA1 cation channel ,Sensitization ,Irritable bowel syndrome ,chemistry.chemical_classification ,business.industry ,Cold temperature ,Gastroenterology ,food and beverages ,Visceral pain ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,TRPA1 Cation Channel ,Original Article ,psychological ,030211 gastroenterology & hepatology ,Neurology (clinical) ,medicine.symptom ,business - Abstract
Background/Aims Abdominal pain can be evoked or exacerbated after gastrointestinal cold stimulation in some patients with diarrhea-predominant irritable bowel syndrome (IBS-D), indicating a low temperature-induced sensitization of visceral perception. We investigated the role of vagal transient receptor potential ankyrin 1 (TRPA1, a cold-sensing ion channel) in cold-aggravated visceral mechanonociception in a stress-induced IBS animal model. Methods TRPA1 expression was examined in antral biopsies of healthy controls and IBS-D patients. Abdominal symptoms were assessed before and after warm or cold water intake. The visceromotor response (VMR) to colorectal distention (CRD) following intra-antral infusion of cold saline was measured in animals undergoing sham or chronic water avoidance stress. TRPA1 expression, extracellular signal-regulated protein kinase 1/2 (ERK1/2) phosphorylation, and neuronal calcium influx in vagal afferents were assessed. Results Compared to healthy controls, IBS-D patients displayed elevated antral TRPA1 expression, which was associated with symptom scores after cold (4°C) water intake. Intra-antral infusion of cold saline increased VMR to CRD in naive rats, an effect dependent on vagal afferents. In stressed rats, this effect was greatly enhanced. Functional blockade and gene deletion of TRPA1 abolished the cold effect on visceral nociception. TRPA1 expression in vagal (but not spinal) afferents increased after stress. Moreover, the cold-induced, TRPA1-dependent ERK1/2 activation and calcium influx in nodose neurons were more robust in stressed rats. Conclusions Stress-exaggerated visceral mechanonociception after antral cold exposure may involve up-regulation of TRPA1 expression and function on vagal afferents. Our findings reveal a novel mechanism for abnormal gastrointestinal cold sensing in IBS.
- Published
- 2019
37. Differential invariants of Camassa–Holm equation
- Author
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Zhong-Yu Su, Wen-Ting Li, Fei Wang, and Wei Li
- Subjects
Camassa–Holm equation ,Recurrence relation ,Infinitesimal ,Mathematical analysis ,General Physics and Astronomy ,Symmetry group ,01 natural sciences ,010305 fluids & plasmas ,Nonlinear system ,Nonlinear Sciences::Exactly Solvable and Integrable Systems ,0103 physical sciences ,Equivariant map ,Invariant (mathematics) ,010306 general physics ,Shallow water equations ,Mathematics - Abstract
Under investigation in this work is a nonlinear shallow water equation, Camassa–Holm equation, which is very important in fluid dynamics, nonlinear dynamics and physical application. The new equivariant moving frames method is implemented to obtain a finite generating set of differential invariants, recurrence relations, and syzygies among the generating differential invariants, for Lie symmetry group of Camassa–Holm equation. This method is very efficient, only using the infinitesimal determining equations and choice of cross-section normalization. The results are useful support for describing the invariant properties and trend of physical, oceanic or atmospheric motion.
- Published
- 2019
38. Musculoskeletal Alterations of Hindlimb During Simulated Microgravity and Reloading in Rats: Compared by Gender
- Author
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Wen-Ting Li, Xiao Yang, Lianwen Sun, Shouhui Wang, Yunfei Huang, and Yubo Fan
- Subjects
Control level ,medicine.medical_specialty ,biology ,business.industry ,Applied Mathematics ,General Engineering ,General Physics and Astronomy ,Hindlimb ,Muscle mass ,01 natural sciences ,010305 fluids & plasmas ,Endocrinology ,Sex hormone-binding globulin ,Simulated microgravity ,Modeling and Simulation ,Internal medicine ,0103 physical sciences ,Male rats ,Sprague dawley rats ,medicine ,biology.protein ,010306 general physics ,business ,Recovery phase - Abstract
Although several researches have reported potential gender differences in adaptations during microgravity, the musculoskeletal alterations of different genders are still under debate. Besides, there were few researches which involved recovery phase after reloading. In the present study, we investigated the musculoskeletal alterations of rats hindlimb via tail-suspension in both genders, and analyzed the serum sex hormone at the same time. Thirty-two Sprague Dawley rats were equally divided by gender into four groups randomly: control of male (CONM), tail-suspension of male (TSM), control of female (CONF) and tail-suspension of female (TSF). The experiment consisted two phases, which were 21 days of tail suspension phase and 21 days’ reloading phase, and parameters of bone, muscle and serum were tested at the end point of each phase. The results indicated that during tail suspension phase, male rats showed a greater and earlier bone loss and microstructure deterioration compared with females, but they could maintain muscle strength much better. In addition, during reloading phase, male rats showed a slower bone recovery and some parameters could not recover until the end, while loss in muscle mass and strength in both genders could recover to control level.
- Published
- 2019
39. Phase-matching quantum key distribution with light source monitoring
- Author
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Wen-Ting Li, Le Wang, Wei Li, and Sheng-Mei Zhao
- Subjects
General Physics and Astronomy - Abstract
The transmission loss of photons during quantum key distribution (QKD) process leads to the linear key rate bound for practical QKD systems without quantum repeaters. Phase matching quantum key distribution (PM-QKD) protocol, an novel QKD protocol, can overcome the constraint with a measurement-device-independent structure, while it still requires the light source to be ideal. This assumption is not guaranteed in practice, leading to practical secure issues. In this paper, we propose a modified PM-QKD protocol with a light source monitoring, named PM-QKD-LSM protocol, which can guarantee the security of the system under the non-ideal source condition. The results show that our proposed protocol performs almost the same as the ideal PM-QKD protocol even considering the imperfect factors in practical systems. PM-QKD-LSM protocol has a better performance with source fluctuation, and it is robust in symmetric or asymmetric cases.
- Published
- 2022
40. Skeleton-based Hand Gesture Recognition for Assembly Line Operation
- Author
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Shang-Che Hsu, Chao-Lung Yang, and Wen-Ting Li
- Subjects
Computer science ,business.industry ,020207 software engineering ,02 engineering and technology ,Gesture recognition ,0202 electrical engineering, electronic engineering, information engineering ,Graph (abstract data type) ,020201 artificial intelligence & image processing ,Computer vision ,Artificial intelligence ,Assembly line ,business ,Network model ,Gesture - Abstract
This research aims to develop a hand gesture recognition (HGR) by combining the OpenPose and Spatial Temporal Graph Convolution Network (ST-GCN) to classify the operator’s assembly motion. By defining the hand gestures with five types of therbligs, the network model was trained to recognize the human hand gesture. Although the accuracy of recognition is 78.3% with room for improvement based on preliminary experiment results, the structure of the proposed network establishes a foundation for further improvement in future work.
- Published
- 2020
41. Acute stress induces visceral hypersensitivity via glucocorticoid receptor–mediated membrane insertion of TRPM8: Involvement of a non‐receptor tyrosine kinase Pyk2
- Author
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Qing-Qing Luo, Xiu-Juan Yan, Bo Wang, Xin Chen, Hong-Yi Qiu, Sheng-Liang Chen, and Wen-Ting Li
- Subjects
Male ,0301 basic medicine ,Physiology ,TRPM Cation Channels ,Rats, Sprague-Dawley ,03 medical and health sciences ,Transient receptor potential channel ,Receptors, Glucocorticoid ,0302 clinical medicine ,Glucocorticoid receptor ,Animals ,Humans ,Protein kinase A ,Protein kinase B ,Cells, Cultured ,Protein kinase C ,PI3K/AKT/mTOR pathway ,Endocrine and Autonomic Systems ,Chemistry ,Gastroenterology ,Visceral Pain ,Rats ,Cell biology ,Focal Adhesion Kinase 2 ,HEK293 Cells ,030104 developmental biology ,Phosphorylation ,Tyrosine kinase ,Stress, Psychological ,030217 neurology & neurosurgery - Abstract
Background Psychological stress is an important factor for the development and recurrence of irritable bowel syndrome (IBS). The mechanisms underlying stress-induced visceral hypersensitivity (VH), a key pathophysiological component in IBS, are still incompletely understood. We aimed to test whether transient receptor potential melastatin 8 (TRPM8) participates in acute stress-induced VH. Methods Rats were subjected to 1-hour water avoidance stress (WAS). Visceral sensitivity was measured with visceromotor response to colorectal distension. Western blot and immunofluorescence were applied to evaluate the expression of GR and TRPM8 and activation of PKA, Akt, and PKC pathways. Results WAS-caused VH depended on glucocorticoid receptors (GRs) and TRPM8 channels. In a dorsal root ganglion (DRG)-derived cell line, corticosterone rapidly (within 30 minutes) induced membrane expression of TRPM8. This effect was inhibited by GR antagonism and was mimicked by membrane-impermeable corticosterone. PKA, PI3K/Akt, and PKC pathways, which lied downstream of GR and acted in parallel to promote membrane expression of TRPM8, contributed to WAS-induced VH. The non-receptor tyrosine kinase Pyk2, which may serve as a convergence point for PKA, PI3K/Akt, and PKC pathways, facilitated membrane insertion of TRPM8 via tyrosine-phosphorylating TRPM8 in L6-S2 DRGs and participated in WAS-induced VH. Conclusions Collectively, acute stress-induced VH could involve membrane-bound GR-dependent enhancement of TRPM8 function in nociceptive DRG neurons. Mechanistically, Pyk2 could act as a key mediator that coordinates multiple protein kinase signaling and triggers phosphorylation and membrane insertion of TRPM8.
- Published
- 2020
42. Biological Mechanism of Specific Relationship between Gan (Liver) Meridian of Foot-Jueyin and Genitals
- Author
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Yu, Zhu, Jian-Peng, Huang, Jia-Ming, Zhong, Wen-Ting, Li, Neng-Gui, Xu, and Jian-Hua, Liu
- Subjects
Liver ,Acupuncture Therapy ,Humans ,Genitalia ,Meridians ,Acupuncture Points - Abstract
From the perspective view of Chinese medicine, the Gan (Liver) meridian of Foot-Jueyin starts from the great toe, running upward along the medial side of the thigh to the perineal area, where it curves around the external genitalia and goes up to the lower abdomen. In clinical practice, acupoints in the feet of the Gan meridian of Foot-Jueyin are used to treat the genitourinary and external genitalia diseases. Studies have shown that reproductive system diseases have specific pathological reactions in the places (radial side of tibia and foot) where Gan meridian of Foot-Jueyin passes by. Why does this happen? In this article, we begin by briefly reviewing the evidences linking foot and genitalia. We then explore the potential mechanism of the relationship between genitals and the Gan meridian of Foot-Jueyin. The brain cerebral cortex is characterized by cortical interactions. Numerous studies show that different cerebral cortex function areas (especially the adjacent areas) are overlapping and interact with each other. Finally, we presume that there is a specific connection between the feet and the genitals. Physiologically in the cortical homunculus, the genital area lies adjacent or overlapped to the foot areas, the two areas may interact with each other. The functional reorganization between different areas of the cerebral cortex under pathological conditions may be the underlying mechanism of the relationship between the feet and the genitals.
- Published
- 2019
43. Targeting RNS/caveolin-1/MMP signaling cascades to protect against cerebral ischemia-reperfusion injuries: potential application for drug discovery
- Author
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Jiangang Shen, Xi Chen, Hansen Chen, and Wen-ting Li
- Subjects
0301 basic medicine ,caveolin-1 ,Cell signaling ,Momordica charantia ,Caveolin 1 ,Ischemia ,Review Article ,Brain damage ,Pharmacology ,Brain Ischemia ,Nitric oxide ,natural compound ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Animals ,Humans ,Medicine ,Pharmacology (medical) ,Reactive nitrogen species ,Ischemic stroke ,biology ,business.industry ,General Medicine ,medicine.disease ,Reactive Nitrogen Species ,Matrix Metalloproteinases ,Stroke ,Nitric oxide synthase ,Neuroprotective Agents ,030104 developmental biology ,chemistry ,Reperfusion Injury ,cardiovascular system ,biology.protein ,reactive nitrogen species (RNS) ,MMPs ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Peroxynitrite ,Signal Transduction - Abstract
Reactive nitrogen species (RNS) play important roles in mediating cerebral ischemia-reperfusion injury. RNS activate multiple signaling pathways and participate in different cellular events in cerebral ischemia-reperfusion injury. Recent studies have indicated that caveolin-1 and matrix metalloproteinase (MMP) are important signaling molecules in the pathological process of ischemic brain injury. During cerebral ischemia-reperfusion, the production of nitric oxide (NO) and peroxynitrite (ONOO−), two representative RNS, down-regulates the expression of caveolin-1 (Cav-1) and, in turn, further activates nitric oxide synthase (NOS) to promote RNS generation. The increased RNS further induce MMP activation and mediate disruption of the blood-brain barrier (BBB), aggravating the brain damage in cerebral ischemia-reperfusion injury. Therefore, the feedback interaction among RNS/Cav-1/MMPs provides an amplified mechanism for aggravating ischemic brain damage during cerebral ischemia-reperfusion injury. Targeting the RNS/Cav-1/MMP pathway could be a promising therapeutic strategy for protecting against cerebral ischemia-reperfusion injury. In this mini-review article, we highlight the important role of the RNS/Cav-1/MMP signaling cascades in ischemic stroke injury and review the current progress of studies seeking therapeutic compounds targeting the RNS/Cav-1/MMP signaling cascades to attenuate cerebral ischemia-reperfusion injury. Several representative natural compounds, including calycosin-7-O-β-D-glucoside, baicalin, Momordica charantia polysaccharide (MCP), chlorogenic acid, lutein and lycopene, have shown potential for targeting the RNS/Cav-1/MMP signaling pathway to protect the brain in ischemic stroke. Therefore, the RNS/Cav-1/MMP pathway is an important therapeutic target in ischemic stroke treatment.
- Published
- 2018
44. Sports Injury-Related Fingers and Thumb Deformity Due to Tendon or Ligament Rupture
- Author
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Yue Liu, Hui-Bo Zhang, Z H Qian, Wen-Ting Li, Nai-Li Wang, Yuming Yin, H L Zhan, and Rong-Jie Bai
- Subjects
Adult ,Male ,musculoskeletal diseases ,Soft Tissue Injuries ,lcsh:Medicine ,Stener Lesion ,Thumb ,Jersey Finger ,030218 nuclear medicine & medical imaging ,Fingers ,Metacarpophalangeal Joint ,03 medical and health sciences ,Mallet Finger ,0302 clinical medicine ,Mallet finger ,Tendon Injuries ,medicine ,Humans ,Orthodontics ,Ligaments ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Magnetic resonance imaging ,030229 sport sciences ,General Medicine ,Boutonniere Deformity ,Magnetic Resonance Imaging ,Hand Deformities ,Middle Aged ,medicine.disease ,musculoskeletal system ,Tendon ,body regions ,medicine.anatomical_structure ,Athletic Injuries ,Ligament ,Original Article ,Female ,business ,Stener lesion ,human activities ,Boutonniere deformity - Abstract
Background: Hand injuries are very common in sports, such as skiing and ball sports. One of the major reasons causing hand and finger deformity is due to ligament and tendon injury. The aim of this study was to investigate if the high-resolution 3T magnetic resonance imaging (MRI) can demonstrate the complex anatomy of the fingers and thumb, especially the tendons and ligaments, and provide the accurate diagnosis of clinically important fingers and thumbs deformity due to ligamentous and tendinous injuries during sport activities. Methods: Sixteen fresh un-embalmed cadaveric hands were harvested from eight cadavers. A total of 20 healthy volunteers' hands and 44 patients with fingers or thumb deformity due to sports-related injuries were included in this study. All subjects had MR examination with T1-weighted images and proton density-weighted imaging with fat suppression (PD FS) in axial, coronal, and sagittal plane, respectively. Subsequently, all 16 cadaveric hands were sliced into 2-mm thick slab with a band saw (six in coronal plane, six in sagittal plane, and four in axial plane). The correlation of anatomic sections and the MRI characteristics of tendons of fingers and the ulnar collateral ligament (UCL) at the metacarpal phalangeal joint (MCPJ) of thumb between 20 healthy volunteers and 44 patients (confirmed by surgery) were analyzed. Results: The normal ligaments and tendons in 16 cadaveric hands and 20 volunteers' hands showed uniform low-signal intensity on all the sequences of the MRI. Among 44 patients with tendinous and ligamentous injuries in the fingers or thumb, 12 cases with UCL injury at MCPJ of the thumb (Stener lesion = 8 and non-Stener lesion = 4), 6 cases with the central slip injury, 12 cases with terminal tendon injury, and 14 cases with flexor digitorum profundus injury. The ligaments and tendons disruption manifested as increased signal intensity and poor definition, discontinuity, and heterogeneous signal intensity of the involved ligaments and tendons. Conclusions: Sports injury-related fingers and thumb deformity are relatively common. MRI is an accurate method for evaluation of the anatomy and pathologic conditions of the fingers and thumb. It is a useful tool for accurate diagnosis of the sports-related ligaments and tendons injuries in hand.
- Published
- 2018
45. Antibacterial constituents from Antarctic fungus, Aspergillus sydowii SP-1
- Author
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Linlin Wang, Dan Luo, Tao Xi, Wen-Ting Li, Jian-Min Liao, Jia-ning Huang, Yuanyuan Lu, and Feng-guo Zhang
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Antarctic Regions ,Microbial Sensitivity Tests ,Plant Science ,Fungus ,Tigecycline ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Microbiology ,Alkaloids ,Staphylococcus epidermidis ,medicine ,Aspergillus sydowii ,Phenylacetates ,Molecular Structure ,biology ,010405 organic chemistry ,Alkaloid ,Organic Chemistry ,biology.organism_classification ,Antimicrobial ,Anti-Bacterial Agents ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Aspergillus ,Staphylococcus aureus ,Sesquiterpenes ,Antarctic microorganism ,medicine.drug - Abstract
One new alkaloid, named as acremolin C (1), was isolated from static culture of Antarctic fungus, Aspergillus sydowii SP-1, in an investigation of the antimicrobial constituents of this Antarctic microorganism, and its structure was determined by spectroscopic methods. Additionally, four known compounds, cyclo-(L-Trp-L-Phe) (2), 4-hydroxyphenylacetic acid (3), (7S)-(+)-hydroxysydonic acid (4) and (7S, 11S)-(+)-12-hydroxysydonic acid (5), were isolated and identified. Biological studies disclosed that compounds 2, 4 and 5 showed moderate inhibitions against methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) as comparing to tigecycline, while compound 1 displayed weak inhibition activities against MRSA and MRSE.
- Published
- 2017
46. High-resolution 3T Magnetic Resonance Imaging of the Triangular Fibrocartilage Complex in Chinese Wrists
- Author
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Nai-Li Wang, Z H Qian, Rong-Jie Bai, Wen-Ting Li, Yuming Yin, W Ye, and H L Zhan
- Subjects
Adult ,Male ,Triangular Fibrocartilage ,Wrist Joint ,Triangular fibrocartilage ,lcsh:Medicine ,High resolution ,Wrist ,Wrist pain ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Magnetic resonance imaging ,General Medicine ,Anatomy ,Middle Aged ,Magnetic Resonance Imaging ,body regions ,Cross-Sectional Studies ,medicine.anatomical_structure ,Triangular Fibrocartilage Complex ,030220 oncology & carcinogenesis ,Cross-Sectional Anatomy ,Original Article ,Female ,medicine.symptom ,business - Abstract
Background: The injury of the triangular fibrocartilage complex (TFCC) is a common cause of ulnar-sided wrist pain. The aim of this study was to investigate if the high-resolution 3T magnetic resonance imaging (MRI) could demonstrate the detailed complex anatomy of TFCC in Chinese. Methods: Fourteen Chinese cadaveric wrists (from four men and three women; age range at death from 30 to 60 years; mean age at 46 years) and forty healthy Chinese wrists (from 20 healthy volunteers, male/female: 10/10; age range from 21 to 53 years with a mean age of 32 years) in Beijing Jishuitan Hospital from March 2014 to March 2016 were included in this study. All cadavers and volunteers had magnetic resonance (MR) examination of the wrist with coronal T1-weighted and proton density-weighted imaging with fat suppression in three planes, respectively. MR arthrography (MRAr) was performed on one of the cadaveric wrists. Subsequently, all 14 cadaveric wrists were sliced into 2 mm thick slab with band saw (six in coronal plane, four in sagittal plane, and four in axial plane). The MRI features of normal TFCC were analyzed in these specimens and forty healthy wrists. Results: Triangular fibrocartilage, the ulnar collateral ligament, and the meniscal homolog could be best observed on images in coronal plane. The palmar and dorsal radioulnar ligaments were best evaluated in transverse plane. The ulnotriquetral and ulnolunate ligaments were best visualized in sagittal plane. The latter two structures and the volar and dorsal capsules were better demonstrated on MRAr. Conclusion: High-resolution 3T MRI is capable to show the detailed complex anatomy of the TFCC and can provide valuable information for the clinical diagnosis in Chinese.
- Published
- 2017
47. Identification of sphingosine kinase 1 (SphK1) as a primary target of icaritin in hepatocellular carcinoma cells
- Author
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Pei-Hua Lu, Mian-Hua Wu, Mu-Xin Wei, Min-Bin Chen, Wen-Ting Li, Shu-Kui Qin, Chaoying Liu, and Yuan-yuan Liu
- Subjects
Male ,0301 basic medicine ,Ceramide ,Carcinoma, Hepatocellular ,Apoptosis ,Mice, SCID ,Traditional Chinese medicine ,Ceramides ,Gene Expression Regulation, Enzymologic ,hepatocellular carcinoma (HCC) ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,sphingosine kinase 1 (SphK1) ,Biomarkers, Tumor ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Neoplasm ,Cytotoxic T cell ,ceramide ,Cell Proliferation ,Flavonoids ,biology ,Traditional medicine ,business.industry ,Liver Neoplasms ,Cancer ,Middle Aged ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,Phosphotransferases (Alcohol Group Acceptor) ,030104 developmental biology ,Oncology ,Sphingosine kinase 1 ,chemistry ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,icaritin ,Cancer research ,biology.protein ,business ,Research Paper ,Signal Transduction - Abstract
// Pei-Hua Lu 1, 2, * , Min-Bin Chen 3, * , Yuan-Yuan Liu 3, * , Mian-Hua Wu 2 , Wen-Ting Li 2 , Mu-Xin Wei 4 , Chao-Ying Liu 1 , Shu-Kui Qin 5 1 Department of Medical Oncology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, China 2 Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor of Nanjing University of Chinese Medicine, Nanjing, China 3 Department of Oncology, Kunshan First People’s Hospital Affiliated to Jiangsu University, Kunshan, China 4 Department of Traditional Chinese Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing, China 5 People’s Liberation Army Cancer Center, 81st Hospital of People’s Liberation Army, Nanjing, China * Co-first authors Correspondence to: Shu-Kui Qin, email: drqinshukuinj@126.com Min-Bin Chen, email: cmb1981@163.com Keywords: hepatocellular carcinoma (HCC), icaritin, sphingosine kinase 1 (SphK1), ceramide Received: September 20, 2016 Accepted: November 08, 2016 Published: February 09, 2017 ABSTRACT Hepatocellular carcinoma (HCC) is a highly aggressive neoplasm. We aim to explore the anti-HCC activity by a natural prenylflavonoid icaritin. Icaritin was cytotoxic and pro-apoptotic when added to established (HepG2, KYN-2 and Huh-7 lines) and primary human HCC cells. At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. SphK1 inhibition or silence (by shRNA/microRNA) mimicked icaritin-mediated cytotoxicity, and almost nullified icaritin’s activity in HepG2 cells. Reversely, exogenous over-expression of SphK1 sensitized icaritin-induced HepG2 cell apoptosis. In vivo , oral administration of icaritin dramatically inhibited HepG2 xenograft growth in SCID mice. Further, SphK1 activity in icaritin-treated tumors was largely inhibited. In summary, icaritin exerts potent anti-HCC activity in vitro and in vivo . SphK1 inhibition could be the primary mechanism of its actions in HCC cells.
- Published
- 2017
48. Association of changes in NT-proBNP, hsTnT and uric acid levels with haemodynamic changes after targeted medical therapies in patients with idiopathic pulmonary arterial hypertension.
- Author
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Wen-ting Li, Chang-wei Wu, and Jin-ming Liu
- Published
- 2022
- Full Text
- View/download PDF
49. Would Interstitial Fluid Flow be Responsible for Skeletal Maintenance in Tail-Suspended Rats?
- Author
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Wen-Ting Li, Lianwen Sun, Yunfei Huang, Bao-Zhang Zhu, Hui-Qin Luan, and Yubo Fan
- Subjects
0301 basic medicine ,Bone mineral ,business.industry ,Weightlessness ,Applied Mathematics ,Osteoporosis ,Ulna ,General Engineering ,General Physics and Astronomy ,Anatomy ,medicine.disease ,Bone tissue ,01 natural sciences ,010305 fluids & plasmas ,03 medical and health sciences ,030104 developmental biology ,Lumbar ,medicine.anatomical_structure ,Modeling and Simulation ,0103 physical sciences ,medicine ,Tibia ,business ,Parietal bone - Abstract
Despite the fast development of manned space flight, the mechanism and countermeasures of weightlessness osteoporosis in astronauts are still within research. It is accepted that unloading has been considered as primary factor, but the precise mechanism is still unclear. Since bone’s interstitial fluid flow (IFF) is believed to be significant to nutrient supply and waste metabolism of bone tissue, it may influence bone quality as well. We investigated IFF’s variation in different parts of body (included parietal bone, ulna, lumbar, tibia and tailbone) of rats using a tail-suspended (TS) system. Ten female Sprague-Dawley (SD) rats were divided into two groups: control (CON) and tail-suspension (TS) group. And after 21 days’ experiment, the rats were injected reactive red to observe lacuna’s condition under a confocal laser scanning microscope. The variations of IFF were analyzed by the number and area of lacuna. Volumetric bone mineral density (vBMD) and microarchitecture of bones were evaluated by micro-CT. The correlation coefficients between lacuna’s number/area and vBMD were also analyzed. According to our experimental results, a 21 days’ tail-suspension could cause a decrease of IFF in lumbar, tibia and tailbone and an increase of IFF in ulna. But in parietal bone, it showed no significant change. The vBMD and microarchitecture parameters also decreased in lumbar and tibia and increased in ulna. But in parietal bone and tailbone, it showed no significant change. And correlation analysis showed significant correlation between vBMD and lacuna’s number in lumbar, tibia and ulna. Therefore, IFF decrease may be partly contribute to bone loss in tail-suspended rats, and it should be further investigated.
- Published
- 2016
50. Artesunate restores spatial learning of rats with hepatic encephalopathy by inhibiting ammonia-induced oxidative damage in neurons and dysfunction of glutamate signaling in astroglial cells
- Author
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Yi Yang, Jiang-ming Zhao, Wen-ting Li, Li Zhang, and Yuan-bo Wu
- Subjects
Male ,0301 basic medicine ,ATPase ,Spatial Learning ,Artesunate ,Glutamic Acid ,Biology ,Pharmacology ,Neuroprotection ,03 medical and health sciences ,chemistry.chemical_compound ,Ammonia ,Cell Line, Tumor ,Glutamine synthetase ,Animals ,Humans ,Viability assay ,Rats, Wistar ,Neurons ,chemistry.chemical_classification ,Reactive oxygen species ,Glutamate receptor ,General Medicine ,Glutathione ,Artemisinins ,Oxidative Stress ,030104 developmental biology ,chemistry ,Biochemistry ,Astrocytes ,Hepatic Encephalopathy ,biology.protein ,Thioacetamide ,Reactive Oxygen Species ,Signal Transduction - Abstract
Background Artesunate (ART) is an antimalarial drug with potential anti-inflammatory effect. This study aimed to explore the potential protective role of ART in hepatic encephalopathy (HE), involving its function against ammonia toxicity. Methods HE rats were induced by the administration of thioacetamide (TAA, 300 mg/kg/day). Spatial learning ability was tested in both Morris water and eight-arm radial maze. Rat cerebellar granule neurons (CGNs) were prepared for ammonia treatment in vitro, in line with SH-SY5Y and C6 cells. ART was administrated at 50 or 100 mg/kg/day in vivo or added at 50 or 100 μM in vitro. Oxidative damages were evaluated by the changes of cell viability, reactive oxygen species (ROS) levels and glutathione (GSH) content, while glutamate uptake and release, and the activities of glutamine synthetase (GS) and Na + K + -ATPase were measured to indicate the dysfunction of glutamate signaling. Results Decreased escape latency and increased numbers of working errors were observed in TAA-induced HE rats, which could be significantly restored by ART at a dosage-dependent manner. Decreased cell viability and GSH content and increased ROS accumulation were detected in ammonia-treated SH-SY5Y and CGNs, while ammonia-treated C6 cells showed reduced glutamate uptake, increased glutamate release, and decrease of GSH content, GS and Na + K + -ATPase activity. In contrast, ART, especially at 100 μM, strongly reversed all changes induced by ammonia, showing a similar dosage-dependent manner in vitro. Conclusion This study revealed a new neuroprotective role of ART in the pathogenesis of HE, by protecting neurons and astroglial cells from ammonia-induced damages and dysfunctions.
- Published
- 2016
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