1. Comparative physiological and transcriptomic analyses of photosynthesis in Sphagneticola calendulacea (L.) Pruski and Sphagneticola trilobata (L.) Pruski
- Author
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Chang-Lian Peng, Qilei Zhang, Min-Ling Cai, Wen-Qiao Ding, Hong-Ying Huang, and Jun-Jie Zhang
- Subjects
0106 biological sciences ,0301 basic medicine ,Stomatal conductance ,China ,Physiology ,lcsh:Medicine ,Sphagneticola calendulacea ,Photosynthetic pigment ,Photosynthesis ,01 natural sciences ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Species Specificity ,Gene Expression Regulation, Plant ,Botany ,Wedelia ,Genetics ,lcsh:Science ,Transpiration ,Plant Proteins ,Multidisciplinary ,biology ,Ecology ,Gene Expression Profiling ,RuBisCO ,lcsh:R ,High-Throughput Nucleotide Sequencing ,Molecular Sequence Annotation ,Biodiversity ,biology.organism_classification ,Photosynthetic capacity ,Plant Leaves ,030104 developmental biology ,chemistry ,biology.protein ,lcsh:Q ,Sphagneticola ,Transcriptome ,Plant sciences ,010606 plant biology & botany ,Peptide Hydrolases - Abstract
Sphagneticola trilobata (L.) Pruski is one of the fast-growing malignant weeds in South China. It has severely influenced local biodiversity and native plant habitat. Photosynthesis is the material basis of plant growth and development. However, there are few reports on the photosynthetic transcriptome of S. trilobata. In this study, S. trilobata had a relatively large leaf area and biomass. The gas exchange parameters per unit area of leaves, including net photosynthetic capacity (Pn), intercellular CO2 (Ci), stomatal conductance (Gs), transpiration rate (Tr), water use efficiency (WUE), photosynthetic pigment and Rubisco protein content were higher than those of the native plant Sphagneticola calendulacea (L.) Pruski. On this basis, the differences in photosynthesis pathways between the two Sphagneticola species were analyzed by using the Illumina HiSeq platform. The sequencing results for S. trilobata and S. calendulacea revealed 159,366 and 177,069 unigenes, respectively. Functional annotation revealed 119,350 and 150,846 non-redundant protein database annotations (Nr), 96,637 and 115,711 Swiss-Prot annotations, 49,159 and 60,116 Kyoto Encyclopedia of Genes and Genomes annotations (KEGG), and 83,712 and 97,957 Gene Ontology annotations (GO) in S. trilobata and S. calendulacea, respectively. Additionally, our analysis showed that the expression of key protease genes involved in the photosynthesis pathway, particularly CP43, CP47, PsbA and PetC, had high expression levels in leaves of S. trilobata in comparison to native species. Physiological and transcriptomic analyses suggest the high expression of photosynthetic genes ensures the high photosynthetic capacity of leaves, which is one of the inherent advantages underlying the successful invasion by S. trilobata.
- Published
- 2020