In order to compare two different floating aquatic plants about the nitrogen removal effect of eutrophic water in Lake Dianchi Caohai, Eichhornia crassipes and Pistia stratiotes were employed to microcosm experiments. Results showed that Eichhornia crassipes and Pistia stratiotes had no obvious difference on the ability of nitrogen enrichment and the total nitrogen removal rate. Under the water total nitrogen (TN), dissolved total nitrogen(DTN), nitrate(NO3--N), ammonium(NH4+-N)initial concentration of 8.40, 7.20, 4.12, 2.59 mg·L-1, Eichhornia crassipes and Pistia crassipes seedlings were 1 kg, the average total nitrogen(TN), dissolved total nitrogen(DTN), nitrate(NO3--N), ammonium(NH4+-N)removal rate of Eichhornia crassipes test group were 74.24%, 76.81%, 87.62%, 80.30%respectively; The average total nitrogen(TN), dissolved total nitrogen(DTN), nitrate(NO3--N), ammonium(NH4+-N)removal rate of Pistiacrassipes test group were 70.10%, 78.89%, 94.77%, 87.64% respectively. The average assimilation away from total nitrogen in water nitrogen loss rate of Eichhornia crassipes and Pistia crassipes test group were 89.39% and 82.33% respectively. The average nitrogen content in the sediment of nitrogen in the water loss rate of Eichhornia crassipes, Pistia crassipes and control test group were 6.94%, 11.64% and 83.51% respectively. Eichhornia crassipes and Pistia crassipes could not only effectively inhibit the formation of sediment, but also could significantly decrease the DO and pH of the water. Although Eichhornia crassipes and Pistia stratiotes can significantly reduce the total nitrogen of water, considering the Pistia stratiotes plant is brittle, so large-scale Eichhornia crassipes planting is a feasible way to control eutrophication of lakes.