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Yang Yang,1,2 Shaoqiong Xie,1 Wencheng Jiang,1 Suwei Tang,1,2 Yuling Shi1,2 1Department of Dermatology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 200443, People’s Republic of China; 2Institute of Psoriasis, School of Medicine, Tongji University, Shanghai, 200092, People’s Republic of ChinaCorrespondence: Yuling Shi; Suwei Tang, Department of Dermatology, Shanghai Skin Disease Hospital, Tongji University School of Medicine, 1278 Baode Road, Jing-an District, Shanghai, 200443, People’s Republic of China, Tel +86-13816213884 ; +86-18017337631, Fax +63833136, Email shiyuling1973@tongji.edu.cn; tangsuwei_1115@126.comObjective: This study aimed to investigate key biomarkers and their molecular pathogenesis in psoriasis.Methods: Differentially expressed genes (DEGs) of datasets (GSE13355, GSE30999, and GSE106992) obtained from Gene Expression Omnibus (GEO) were identified using Venn diagram. Function and pathway enrichment analyses were performed. Protein–protein interaction (PPI) network and the hub genes were constructed. The correlation between normal tissue and infiltrating immune cells was analyzed by CIBERSORT. ROC analysis was performed to distinguish between skin lesion samples and skin non-lesion samples. Analyze the highest expression of single gene in the whole body within the Human Protein Atlas (HPA) database. Effect of CXCL8 expression level on proliferation, invasion, migration and apoptosis of HaCat cells was detected by qPCR.Results: A total of 239 pairs of normal and lesional skin samples were downloaded. PPI network revealed a tight interaction among 197 DEGs. The GO enrichment analysis showed that these genes were markedly enriched in the “defense response to virus”, “type I interferon signaling pathway”, and “cell response to type I interferon” categories. The KEGG pathway analysis showed that the DEGs were mainly in the NOD-like receptor axis, interaction between cytokine and cytokine receptor and the IL− 17 axis. PPI analysis showed that CXCL8 was the novel hub gene of psoriasis and correlated to 22 types of infiltrating immune cells. 6 miRNAs were predicted to be related to CXCL8. CXCL8 was most widely distributed in lymphoid tissues and plays a role in psoriatic inflammatory lesions by promoting cell proliferation, migration, and anti-apoptosis.Conclusion: CXCL8 plays a key role in psoriasis development. This study provided new insights into the exploration of molecular mechanisms and therapeutic targets of psoriasis.Keywords: psoriasis, CXCL8, molecular mechanism, bioinformatics

Background and Aims Psoriasis is a relatively common autoimmune inflammatory skin disease with a chronic etiology. Since psoriasis is still incurable, it is necessary to identify the molecular mechanisms of psoriasis. The present study was designed to detect novel biomarkers and pathways associated with psoriasis incidence, and provide new insights into treatment of psoriasis. Methods and Results Differentially expressed genes (DEGs) associated with psoriasis in the Gene Expression Omnibus (GEO) database were identified, and their functional roles and interactions were then annotated and evaluated through GO, KEGG, and gene set variation (GSVA) analyses. In total 197 psoriasis-related DEGs were identified and found to primarily be associated with the NOD-like receptor, IL-17, and cytokine-cytokine receptor interaction signalling pathways. GSVA revealed significant differences between normal and lesional groups (P < 0.05), while PPI network analyses identified CXCL10 as the hub gene with the highest degree value, whereas IRF7, IFIT3, OAS1, GBP1, and ISG15 were promising candidate genes for the therapeutic treatment of psoriasis. Conclusion The findings of the present integrated bioinformatics may enhance our understanding of the molecular events occurring in psoriasis, and these candidate genes and pathways together may prove to be therapeutic targets for psoriasis.

Qi Zheng,1,&ast; Le Kuai,2,&ast; Wencheng Jiang,1,&ast; Yan Qiang,3 Lei Wei,2 Siting Chen,2 Bin Li,1 Ruiping Wang3,4 1Department of Traditional Chinese Medicine, Shanghai Skin Diseases Hospital, Tongji University, Shanghai, People’s Republic of China; 2Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 3Clinical Research and Innovation Transformation Center, Shanghai Skin Diseases Hospital, Tongji University, Shanghai, People’s Republic of China; 4School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Bin Li; Ruiping Wang, Email 18930568129@163.com; w19830901@126.comBackground: Gender difference is prevalent in clinical feature, disease severity for noncommunicable diseases (NCD), but studies on gender disparity in clinical feature, disease severity and NCD comorbidity among psoriasis patients are limited. This cross-sectional study explores gender differences in clinical feature, lifestyle behavior and NCD comorbidity among psoriasis patients.Methods: Psoriasis patients were recruited through cluster survey method in two hospitals, and questionnaire interviews were applied to collect the demographic feature, lifestyle habits, clinical feature and NCD among patients.Results: A total of 2102 psoriasis patients included 1332 males (63.4%), 70% were over 35 years old and approximately 50% of them were overweight or obesity. The median value for psoriasis initiation age and disease duration was 33 years old (34 for male and 32 for female) and 9 years (10 for male and 7 for female), respectively. The psoriasis recurrence was mainly in winter (73.4%) and autumn (34.2%) both for patients. The prevalence of tobacco smoking and alcohol drinking was 31.2% and 12.6%. Male patients had higher prevalence of tobacco smoking (odds ratio (OR) = 13.26, 95% confidence interval (CI): 9.54– 18.44) and alcohol drinking (OR = 14.44, 95% CI: 7.90– 26.40). The prevalence of diabetes, hypertension, hyperlipidemia, and metabolic syndrome were 13.2%, 28.5%, 23.4% and 21.5%, respectively. Male patients had higher prevalence of diabetes (OR = 1.53, 95% CI: 1.16– 2.02), hypertension (OR = 1.87, 95% CI: 1.52– 2.30), hyperlipidemia (OR = 2.34, 95% CI: 1.85– 2.95) and metabolic syndrome (OR = 2.06, 95% CI: 1.63– 2.62) than female patients. The proportions for 4 types of NCDs diagnosed after psoriasis onset were over 58%, which were also higher in males than females.Conclusion: Female patients had shorter disease duration and with less NCD, and male patients had more body weight issue, with fewer sleep time and higher prevalence of tobacco smoking and alcohol drinking and NCDs. We recommend that dermatologist should notice the gender disparity in psoriasis patients, which is helpful for the disease diagnosis and treatment.Keywords: psoriasis, gender disparity, tobacco smoking, alcohol drinking, overweight, metabolic diseases, comorbidity

Introduction A number of studies have reported a high correlation between psoriasis and metabolic syndrome (MetS), and tobacco smoking is one independent risk factor accounting for the increased prevalence both for psoriasis and MetS. However, few studies have been conducted to assess the effects of tobacco smoking on co-morbidities of psoriasis and MetS. Methods We conducted a cross-sectional study with 1014 psoriasis patients recruited from January to May 2021. Patients were recruited with a cluster survey method in Yueyang Hospital (affiliated with Shanghai University of Traditional Chinese Medicine) and Shanghai Skin Diseases Hospital (affiliated with Tongji University). Data were collected by face-to-face questionnaire interviews which included basic information, personal life habits, medical history, and clinical examinations. SPSS 24.0 was used for data analysis and a p

Citron kinase (CIT) is a Rho-effector protein kinase that is associated with several types of cancer. However, the role of CIT in prostate cancer (PCa) is unclear. The current study utilized microarray data obtained from The Cancer Genome Atlas, which was analyzed via Biometric Research Program array tools. Additionally, reverse transcription-quantitative (RT-q)PCR was performed to compare the mRNA expression of CIT in PCa tissue and in benign prostatic hyperplasia. The protein expression of CIT was detected in a consecutive cohort via immunochemistry and CIT was screened as a potential oncogene in PCa. The results of RT-qPCR demonstrated that the mRNA expression of CIT was increased in PCa tissues. Furthermore, immunochemistry revealed that CIT protein expression was positively associated with age at diagnosis, Gleason grade, serum PSA, clinical T stage, risk group, lymph node invasion and metastasis. When compared with the low expression group, patients with a high CIT expression exhibited shorter survival rates, cancer specific mortalities (CSM) and biochemical recurrence (BCR). In addition, multivariate analysis revealed that CIT was a potential predictor of CSM and BCR. The results revealed that CIT is overexpressed during the malignant progression of PCa and may be a predictor of a poor patient prognosis.

This study examines the 4 typical natural plant communities at the coastal waterfront of Jiaozhou Bay. Based on field survey and sampling, laboratory analysis, application of classical statistical methods and the requirements for urban ecological preservation and data restoration in IoT engineering technology, this study investigates the diversity pattern of the 4 natural plant communities, i.e. Phragmitesaustralis, Suaeda salsa, Setariaviridis and Conyzacanadensis, analyzes the characteristics of the petroleum pollution suffered by these 4 natural plant communities and the soil at the coastal waterfront of Jiaozhou Bay. By investigating the impact on the natural regulation ability of the natural ecosystem, species and habitat, this study provides the basis for protection of the natural plant communities in the petroleum polluted area and for the design of plants arrangement in ecological restoration in the site and provides the support for application of IoT technology in urban ecosystem establishment and urban ecological restoration. Findings: (1) During investigation of 30 quadrats of the plant communities in the petroleum polluted area at coastal waterfront of Qingdao, 74 plant species have been discovered.They belong to 12 families, where Chenopodiaceae, Poaceae and Compositae are dominant families and account for 9.09%, 20.45% and 38.63% respectively; Phragmitesaustralis, Suaeda salsa, Conyza canadensis and Setariaviridis communities are all herbosa, their species composition is quite simplex and most of them are in monodominant community patch; (2) The Conyzacanadensis community has the largest Shannon-Wiener diversity index, Patrick richness index and Simpson dominance index, which are 2.115, 1.930 and 1.228 respectively; in terms Pielou evenness index, the rank is Phragmitesaustralis Conyzacanadensis Setariaviridis Suaeda salsa, which are 1.056, 0.907, 0.877 and 0.661; (3) According to the result of data analysis, the petroleum polluted area at coastal waterfront of Jiaozhou Bay has average total salt content 0.626%, average PH 8.067 and average petroleum hydrocarbons content 69.306 mg/kg.According to the result of difference examination of different plant communities, the total salt, PH and petroleum hydrocarbons content in the soil at the coastal waterfront Jiaozhou Bay are the main factors that affect the distribution of plant communities in that area (P 0.05), and the petroleum hydrocarbons content in the soil has significant impact on the distribution of the communities (P 0.01).(4) In this study, the petroleum hydrocarbon tolerance degrees of the 4 typical tolerant plant communities are ranked as Conyzacanadensis Suaeda salsa Setariaviridis Phragmitesaustralis.In the soil environment with light (Level Ⅰ) and severe (Level Ⅳ) petroleum hydrocarbon pollution, the indexes of Suaeda salsa and Conyzacanadensis are optimal among the communities; the indexes of Phragmitesaustralis and Setariaviridis are optimal in the soil environment with Level Ⅱ and Level Ⅲ petroleum hydrocarbon pollution and can grow better in that environment.

Urine-derived stem cells (USC) are isolated from voided urine and have demonstrated potential for use in tissue engineering and regenerative medicine therapies. Clear cell renal cell carcinoma (ccRCC) is a common urological malignancy that originates in the kidney. Since USC also originate in the kidney, the objective of this study was to investigate any biological differences between USC isolated from healthy patients and those isolated from ccRCC patients (rc-USC). We found that USC can be isolated from the voided urine of ccRCC patients (rc-USC) and have a morphology and function similar to those isolated from healthy donors. However, the rc-USC showed greater proliferation and invasion capacity than USC, and possessed some features of cancer cells; but the rc-UC were not able to form xenografts when implanted in vivo. We further performed RNA sequencing of rc-USC and USC and found several differentially expressed lncRNAs and mRNAs; however subsequent GO and KEGG enrichment analysis showed few pathway differences between these cells. Bioinformatic analyses and RT-PCR showed the expression of several known ccRCC-related genes in rc-USC expressed, as compared to USC derived from healthy donors. This study demonstrates that rc-USC displayed several cellular and genetic features of ccRCC cells, which suggests that this population of cells could provide a non-invasive approach for for the diagnosis, predication, disease modeling and therapeutic strategies targeting ccRCC.

Large eddy simulation (LES) is applied to simulate the flow field around a submarine with full appendages. The predicted ship resistance, velocity distributions and surface pressures are validated by experimental data. Dynamic velocity responses of the structure are obtained by solving the fluid-solid coupling equations. The flow noise and flow-excited noise of the submarine are predicted using the boundary element method (BEM). Flow noise predicted by the BEM and the Ffowcs Williams-Hawkings (FW-H) equations are compared and discussed. The spectra of flow-excited noise are different from those of flow noise, and the sound pressure level of flow-excited noise is larger than that of flow noise, especially when structural resonance occurs. Flow-excited noise rapidly decreases in the near-field and maintains a slow attenuation rate in the far-field. The influence of ship speed on flow-excited noise is investigated, and results show that the spectral characteristics of flow-excited noise of the submarine change greatly with different sailing speeds. To achieve noise reduction, different shell thicknesses and different numbers of longitudinal girders are investigated. It is found that increasing the shell thickness and the number of longitudinal girders can indeed reduce noise emission. It should be noted that the noise reduction effect might differ.

We performed a comprehensive meta-analysis to determine the association between XPD/ERCC2 Lys751Gln polymorphism and melanoma susceptibility. Based on comprehensive searches of the MEDLINE, EMBASE and ISI Web of knowledge, China National Knowledge Infrastructure (CNKI) and Wanfang Database, we identified eligible studies about the association between XPD/ERCC2 Lys751Gln polymorphism and melanoma risk. A total of 5,961 cases and 8,669 controls in studies were included in this meta-analysis. All studies were conducted in Caucasian populations. Allele model (Lys vs. Gln: P = 0.53; OR = 0.98, 95% CI = 0.91-1.05), and homozygous model (Lys/ Lys vs. Gln/Gln: P = 0.32; OR = 0.93, 95% CI = 0.81 to 1.07) did not show increased risk of developing melanoma. Similarly, dominant model (Lys/ Lys+Lys/Gln vs. Gln/Gln: P = 0.18; OR = 0.93, 95% CI = 0.83 to 1.03) and recessive model (Lys/ Lys vs. Lys/Gln+Gln/Gln: P = 0.73; OR = 0.98, 95% CI = 0.88 to 1.09) failed to show increased risk of developing melanoma. Our pooled data suggest that there was no evidence for a major role of XPD/ERCC2 Lys751Gln polymorphism in the pathogenesis of melanoma among Caucasian populations.