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28 results on '"Wendy Erber"'

1. Hepcidin predicts response to IV iron therapy in patients admitted to the intensive care unit: a nested cohort study

Author

Edward Litton, Stuart Baker, Wendy Erber, Shannon Farmer, Janet Ferrier, Craig French, Joel Gummer, David Hawkins, Alisa Higgins, Axel Hofmann, Bart De Keulenaer, Julie McMorrow, John K. Olynyk, Toby Richards, Simon Towler, Robert Trengove, Steve Webb, on behalf of the IRONMAN Study investigators, and the Australian and New Zealand Intensive Care Society Clinical Trials Group

Subjects
Anaemia, Critical care, Hepcidin, Intravenous iron, Red blood cell transfusion, Medical emergencies. Critical care. Intensive care. First aid, RC86-88.9
Abstract

Abstract Background Both anaemia and red blood cell (RBC) transfusion are common and associated with adverse outcomes in patients admitted to the intensive care unit (ICU). The aim of this study was to determine whether serum hepcidin concentration, measured early after ICU admission in patients with anaemia, could identify a group in whom intravenous (IV) iron therapy decreased the subsequent RBC transfusion requirement. Methods We conducted a prospective observational study nested within a multicenter randomized controlled trial (RCT) of IV iron versus placebo. The study was conducted in the ICUs of four tertiary hospitals in Perth, Western Australia. Critically ill patients with haemoglobin (Hb) of

Published
2018
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2. Comparison of Circulating Tumour DNA and Extracellular Vesicle DNA by Low-Pass Whole-Genome Sequencing Reveals Molecular Drivers of Disease in a Breast Cancer Patient

Author

Olivia Ruhen, Bob Mirzai, Michael E. Clark, Bella Nguyen, Carlos Salomon, Wendy Erber, and Katie Meehan

Subjects
breast cancer, biomarkers, extracellular vesicle DNA, circulating tumour DNA, liquid biopsy, Biology (General), QH301-705.5
Abstract

There is increasing recognition of circulating tumour DNA (ctDNA) as a non-invasive alternative to tumour tissue for the molecular characterisation and monitoring of disease. Recent evidence suggests that cancer-associated changes can also be detected in the DNA contained within extracellular vesicles (EVs). As yet, there has been limited investigation into the relationship between EV DNA and ctDNA, and no studies have examined the EV DNA of breast cancer patients. The aim of this study was to use low-pass whole-genome sequencing to identify copy number variants (CNVs) in serial samples of both ctDNA and EV DNA from a patient with breast cancer. Of the 52 CNVs identified in tumour DNA, 36 (69%) were detected in at least one ctDNA sample and 13 (25%) in at least one EV DNA sample. The number of detectable variants in ctDNA and EV DNA increased over the natural history of the patient’s disease, which was associated with progression to cerebral metastases. This case study demonstrates that, while CNVs are detectable in patient EV DNA, ctDNA has greater sensitivity than EV DNA for serial monitoring of breast cancer.

Published
2020
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3. Improved classification of leukemic B-cell lymphoproliferative disorders using a transcriptional and genetic classifier

Author

Alba Navarro, Guillem Clot, Alejandra Martínez-Trillos, Magda Pinyol, Pedro Jares, Blanca González-Farré, Daniel Martínez, Nicola Trim, Verónica Fernández, Neus Villamor, Dolors Colomer, Dolors Costa, Itziar Salaverria, David Martín-Garcia, Wendy Erber, Cristina López, Sandrine Jayne, Reiner Siebert, Martin J. S. Dyer, Adrian Wiestner, Wyndham H. Wilson, Marta Aymerich, Armando López-Guillermo, Àlex Sánchez, Elías Campo, Estella Matutes, and Sílvia Beà

Subjects
Diseases of the blood and blood-forming organs, RC633-647.5
Published
2017
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4. Supplementary Table 4 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 105K, Biological processes and genes differentially expressed among M-MCL (M) and U-MCL (U) validated genes by qPCR

Published
2023
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5. Supplementary Table 1 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 105K, Normal B-cell types signatures used in the GSEA analysis

Published
2023
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6. Supplementary Figure 3 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 332K, Determination of the best cut-off for the percentage of identity of IGHV gene in relation to overall survival. Two cut-offs, 96.6 and 97%, were selected according to a maximally log-rank statistic

Published
2023
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7. Supplementary Table 2 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 75K, IGHV, IGHD and IGHJ gene frequencies according to mutational status

Published
2023
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8. Supplementary Table 3 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 108K, P-values of the 518 genes differentially expressed between M-MCL and U-MCL

Published
2023
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9. Supplementary Figure 2 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 198K, Recurrent mutations in rearrangements utilizing the six most frequent IGHV genes. Individual codons are represented in the X-axis. Each color represents a different amino acid (AA) change. CDR: complementary determining region, FR: framework region

Published
2023
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10. Supplementary Figure 1 from Molecular Subsets of Mantle Cell Lymphoma Defined by the IGHV Mutational Status and SOX11 Expression Have Distinct Biologic and Clinical Features

Author

Elías Campo, Sílvia Beà, Kostas Stamatopoulos, Armando Lopez-Guillermo, Reiner Siebert, Eva Giné, Dolors Colomer, Andreas Rosenwald, German Ott, Birgitta Sander, Wendy Erber, Nicola Trim, Maria Jose Calasanz, Christiane Pott, Wyndham Wilson, Adrian Wiestner, Lenka Stefancikova, Alejandra Martinez-Trillos, Virgina Amador, Maria Carmela Vegliante, Jara Palomero, Xavier Puig, Magda Pinyol, Itziar Salaverria, Theodora Papadaki, Nikos Darzentas, Vasilis Bikos, Andreas Agathangelidis, Anastasia Hadzidimitriou, Pedro Jares, Cristina Royo, Guillem Clot, and Alba Navarro

Abstract

PDF file - 73K, SOX11 expression evaluated by three different techniques. One-hundred-sixty-one MCL patients were studied, 64 by immunohistochemistry (IHC), 143 by quantitative PCR (qPCR) and 50 by gene expression profiling (GEP). Several samples were evaluated with more than one technique, and 8 patients were assessed by all three methodologies. The results were concordant in all cases

Published
2023
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11. Immunocytochemical Labelling of Haematological Samples Using Monoclonal Antibodies

Author

Wendy Erber

Subjects
Staining and Labeling, QH301-705.5, Antibodies, Monoclonal, General Medicine, Hematology, immunoenzymatic, Alkaline Phosphatase, Immunohistochemistry, humanities, Commentary, diagnostics, Humans, monoclonal antibodies, Biology (General)
Abstract

I reflect on my experience working with David Y. Mason in the Leukaemia Research Laboratories in the Nuffield Department of Pathology at the University of Oxford in the early 1980s. This was soon after the first monoclonal antibodies had been produced, which led to an exciting and productive time in biological discovery and pathology diagnostics. A specific focus in the laboratory was the development of immunoenzymatic staining methods that would enable monoclonal antibodies to be applied in diagnostic practice. This paper describes the work that led to the performance of immuno-alkaline phosphatase staining on blood and bone marrow smears, the success of which changed leukaemia diagnosis.

Published
2022

12. Loss of function variant in SMIM1 is associated with reduced energy expenditure and weight gain

Author

Mattia Frontini, Luca Stefanucci, Camous Moslemi, Ana Tome, Samuel Virtue, Nicholas Gleadall, Laura Watson, Jing Kwa, Frances Burden, Samantha Farrow, Ji Chen, Urmo Võsa, Keith Burling, Peter Baker, James Warner, Amy Frary, Karola Rehnstrom, Sofie Ashford, Jo Piper, Gail Biggs, Wendy Erber, Gary Hoffman, Nadia Schoenmakers, Christian Erikstrup, Klaus Rieneck, Morten Dziegiel, Henrik Ullum, Vian Azzu, Michele Vacca, Omer Ali Bayraktar, Antonio Vidal-Puig, Sisse Ostrowski, William Astle, Martin L. Olsson, Jill R. Storry, Ole Pedersen, Willem Ouwehand, Krishna Chatterjee, and Dragana Vuckovic

Abstract

Blood group antigens are the archetypal example of human genetic variation. Here, we characterised the functional metabolic consequences in individuals homozygous for a 17bp deletion in SMIM1 (rs566629828; minor allele frequency 0.0147) and thus lacking the protein defining the Vel blood group. Our analysis, in separate cohorts of SMIM1-/- individuals (UK Biobank, NHS Blood and Transplant, Danish Blood Donor Study, Copenhagen Hospital Biobank) and a mouse model, identified an increase in body weight accompanied by a range of metabolic differences, including dyslipidemia, changes in the leptin-adiponectin ratio, increased liver enzymes and lower total thyroid hormone levels. These changes in the metabolic state were at least in part due to a reduction in resting energy expenditure, as assessed during an in-depth clinical assessment of SMIM1-/- individuals. Additionally, electronic health records suggest that individuals lacking this 78-amino-acid type II transmembrane protein may be more prone to cerebral bleeds and thrombotic stroke.

Published
2022
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14. Guidelines on the use of multicolour flow cytometry in the diagnosis of haematological neoplasms. British Committee for Standards in Haematology

Author

Ulrika, Johansson, David, Bloxham, Stephen, Couzens, Jennifer, Jesson, Ricardo, Morilla, Wendy, Erber, and Marion, Macey

Subjects
Quality Control, Blood Cells, Antibodies, Neoplasm, Drug Storage, Lasers, Color, Reproducibility of Results, Bone Marrow Examination, Equipment Design, Flow Cytometry, United Kingdom, Immunophenotyping, Specimen Handling, Antigen-Antibody Reactions, Antigens, Neoplasm, Hematologic Neoplasms, Calibration, Medical Laboratory Personnel, Humans, Indicators and Reagents, Forms and Records Control, Fluorescent Dyes
Published
2014

15. EGFL7 is expressed in bone microenvironment and promotes angiogenesis via ERK, STAT3, and integrin signaling cascades

Author

Shek Man, Chim, Vincent, Kuek, Siu To, Chow, Bay Sie, Lim, Jennifer, Tickner, Jinmin, Zhao, Rosa, Chung, Yu-Wen, Su, Ge, Zhang, Wendy, Erber, Cory J, Xian, Vicki, Rosen, and Jiake, Xu

Subjects
Male, STAT3 Transcription Factor, Vascular Endothelial Growth Factor A, EGF Family of Proteins, Integrins, Osteocalcin, Neovascularization, Physiologic, Osteoclasts, Salter-Harris Fractures, Bone and Bones, Rats, Sprague-Dawley, Mice, Cell Movement, Osteogenesis, Animals, Growth Plate, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Cells, Cultured, Fracture Healing, Osteoblasts, Calcium-Binding Proteins, Endothelial Cells, Proteins, Recombinant Proteins, Rats, Mice, Inbred C57BL, Focal Adhesion Protein-Tyrosine Kinases, Signal Transduction
Abstract

Angiogenesis plays a pivotal role in bone formation, remodeling, and fracture healing. The regulation of angiogenesis in the bone microenvironment is highly complex and orchestrated by intercellular communication between bone cells and endothelial cells. Here, we report that EGF-like domain 7 (EGFL7), a member of the epidermal growth factor (EGF) repeat protein superfamily is expressed in both the osteoclast and osteoblast lineages, and promotes endothelial cell activities. Addition of exogenous recombinant EGFL7 potentiates SVEC (simian virus 40-transformed mouse microvascular endothelial cell line) cell migration and tube-like structure formation in vitro. Moreover, recombinant EGFL7 promotes angiogenesis featuring web-like structures in ex vivo fetal mouse metatarsal angiogenesis assay. We show that recombinant EGFL7 induces phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), signal transducer and activator of transcription 3 (STAT3), and focal adhesion kinase (FAK) in SVEC cells. Inhibition of ERK1/2 and STAT3 signaling impairs EGFL7-induced endothelial cell migration, and angiogenesis in fetal mouse metatarsal explants. Bioinformatic analyses indicate that EGFL7 contains a conserved RGD/QGD motif and EGFL7-induced endothelial cell migration is significantly reduced in the presence of RGD peptides. Moreover, EGFL7 gene expression is significantly upregulated during growth plate injury repair. Together, these results demonstrate that EGFL7 expressed by bone cells regulates endothelial cell activities through integrin-mediated signaling. This study highlights the important role that EGFL7, like EGFL6, expressed in bone microenvironment plays in the regulation of angiogenesis in bone.

Published
2013

16. Monocyte activation in platelet concentrates

Author

Dianne Grey and Wendy Erber

Subjects
Blood Platelets, Interleukin-6, Receptors, IgG, Lipopolysaccharide Receptors, Hematology, General Medicine, Monocytes, Blood Preservation, Plasticizers, Cell Adhesion, Product Packaging, Cytokines, Humans, Polyvinyl Chloride, Biomarkers
Published
2000

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