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25 results on '"Weng, Hongbiao"'

17. Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori

Author

Shen WeiFeng, Meng ZhiQi, Weng Hongbiao, Niu Baolong, Liu Yan, and He Lihua

Subjects
Molecular Sequence Data, Molecular cloning, Gene Expression Regulation, Enzymologic, Protein Structure, Secondary, Article, Bombycidae, eye pigmentation, vermilion gene, Bombyx mori, Complementary DNA, Animals, Amino Acid Sequence, Cloning, Molecular, Peptide sequence, Gene, Phylogeny, Base Sequence, Molecular mass, biology, Gene Expression Profiling, fungi, General Medicine, Bombyx, biology.organism_classification, Eye pigmentation, Molecular biology, Tryptophan Oxygenase, Lepidoptera, Biochemistry, Insect Science, Sequence Alignment
Abstract

A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods.

Published
2008

23. Complete mitochondrial genome of the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae).

Author

Li, Fengbo, Zhang, Huixian, Wang, Wei, Weng, Hongbiao, and Meng, Zhiqi

Subjects
BEETLES, CERAMBYCIDAE, GENOMES, MITOCHONDRIA, MONOCHAMUS
Abstract

We determined the complete mitochondrial genome of the Japanese pine sawyerMonochamus alternatusHope (Coleoptera: Cerambycidae), which is a major forest pest in Asia. The genome is 15,874 bp in length containing 37 typical animal mitochondrial genes and one non-coding A+T-rich region. Its gene content and order are typical of other coleopteran mitochondrial genomes described to date. All protein-coding genes (PCGs) are initiated by ATN codons. Eight PCGs use complete stop codons TAG or TAA, whereas other PCGs end with a single T. All tRNA genes show typical secondary cloverleaf structures except fortRNASer(AGN), which lacks the dihydrouridine (DHU) arm. The large non-coding A+T-rich region of 1249 bp contains a 14 bp-long poly-T stretch and two microsatellite-like (AT)(TA)7and (TA)8elements. [ABSTRACT FROM AUTHOR]

Published
2016
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24. Germline Transformation of the Silkworm Bombyx moriL. by Sperm-Mediated Gene Transfer1

Author

Zhou, Wenlin, Gong, Chengliang, Xue, Renyu, Cao, Guangli, Cao, Jinru, Ye, Aihong, Weng, Hongbiao, and Wang, Yongqiang

Abstract

The domesticated silkworm Bombyx moriL. has important roles in basic biological research and applied science. To explore the practical use of transgenic technology in agricultural silkworm varieties, we fused the neomycin-resistance gene (NeoR) and the green fluorescent protein gene (gfp) into the piggyBac-based transposon vector and transduced it into silkworms by sperm-mediated gene transfer (SMGT). Fluorescence observation indicated the positive rate of G0 egg-batches is 72.7%. After screening against the antibiotic G418, development of individual larvae in the same brood showed significant size differences. PCR detection indicated the existence of gfpand NeoRand confirmed the positive rate of transgenesis as 0.47%. Southern blot analysis confirmed the presence of the exogenous genes in the genome of G7 larvae. These results show that our strategy is practical and markedly improves the efficiency of SMGT.

Published
2012
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25. Complete mitochondrial genome of the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae).

Author

Li F, Zhang H, Wang W, Weng H, and Meng Z

Subjects
Animals, Base Sequence, Coleoptera, DNA, Mitochondrial genetics, Insect Proteins genetics, Mitochondrial Proteins genetics, Molecular Sequence Data, RNA genetics, RNA, Mitochondrial, RNA, Ribosomal genetics, RNA, Transfer genetics, Genes, Mitochondrial physiology, Genome, Mitochondrial physiology
Abstract

We determined the complete mitochondrial genome of the Japanese pine sawyer Monochamus alternatus Hope (Coleoptera: Cerambycidae), which is a major forest pest in Asia. The genome is 15,874 bp in length containing 37 typical animal mitochondrial genes and one non-coding A+T-rich region. Its gene content and order are typical of other coleopteran mitochondrial genomes described to date. All protein-coding genes (PCGs) are initiated by ATN codons. Eight PCGs use complete stop codons TAG or TAA, whereas other PCGs end with a single T. All tRNA genes show typical secondary cloverleaf structures except for tRNA(Ser(AGN)), which lacks the dihydrouridine (DHU) arm. The large non-coding A+T-rich region of 1249 bp contains a 14 bp-long poly-T stretch and two microsatellite-like (AT)(TA)7 and (TA)8 elements.

Published
2016
Full Text
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