230 results on '"Wengang Wang"'
Search Results
2. Isolating switch state detection system based on depth information guidance
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Hui Xu, Xinyang Zhao, Qiyun Yin, Junting Dou, Ruopeng Liu, and Wengang Wang
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isolating switch ,depth information guidance ,saliency object detection ,conditional random field ,state detection ,Mathematics ,QA1-939 ,Applied mathematics. Quantitative methods ,T57-57.97 - Abstract
This study addressed the critical role of isolating switches in controlling circuit connections for the stable operation of the substation. Our research introduced an innovative state detection system that utilized depth information guidance, comprising a controllable pan-tilt mechanism, a depth camera, and an industrial computer. The software component employed a two-stage strategy for precise isolating switch detection. Initially, the red green blue with depth (RGB-D) saliency network identified the approximate area of the isolating switch target. Subsequently, a fully connected conditional random field was applied to extract accurate detection results. The real-time state of the isolating switch was determined based on the geometric relationship between its arms. This approach enhanced the accuracy of isolating switch detection, ensuring practical applicability in engineering scenarios. The significance of this research lies in its contribution to advancing isolating switch monitoring through depth information guidance, promoting a more robust and reliable power system. The key improvement is implementing a two-stage strategy, combining RGB-D saliency analysis and conditional random field processing, resulting in enhanced accuracy in isolating switch detection. As validated through extensive experiments, the proposed system's successful application in practical engineering underscores its effectiveness in meeting the accuracy requirements for isolating switch detection and state detection. This innovation holds promise for broader applications in power systems, showcasing its potential to elevate the reliability and efficiency of electrical networks. Code of the proposed system is available at: https://github.com/miaomiao0909/Isolating-Switch-Detection/tree/master.
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- 2024
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3. FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1–YTHDF2 dependent manner
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Wengang Wang, Xueming Du, Ming Luo, and Ningning Yang
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FTO ,m6A demethylation ,Muscle fiber remodeling ,Adolescent idiopathic scoliosis ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Adolescent idiopathic scoliosis (AIS) is characterized by low lean mass without vertebral deformity. The cause-and-effect relationship between scoliosis and paraspinal muscle imbalance has long puzzled researchers. Although FTO has been identified as a susceptibility gene for AIS, its potential role in the asymmetry of paraspinal muscles has not been fully elucidated. Methods We investigated the role of Fto in murine myoblast proliferation, migration, and myogenic differentiation. We examined its precise regulatory influence on murine muscle fiber remodeling in vitro and in vivo. We identified the downstream target gene of Fto by screening key regulators of murine muscle fiber remodeling and identified its m6A reader. Deep paraspinal muscle samples were obtained from the concave and convex sides of AIS patients with or without Schroth exercises, and congenital scoliosis served as a control group. We compared the content of type I fibers, expression patterns of fast- and slow-type genes, and levels of FTO expression. Results FTO contributed to maintain the formation of murine slow-twitch fibers both in vitro and in vivo. These effects were mediated by the demethylation activity of FTO, which specifically demethylated NFATC1 and prevented YTHDF2 from degrading it. We found a significant reduction in type I fibers, mRNA levels of MYH7 and MYH7B, and expression of FTO on the concave side of AIS. The percentage of type I fibers showed a positive correlation with the expression level of FTO. The asymmetric patterns observed in AIS were consistent with those seen in congenital scoliosis, and the asymmetry of FTO expression and fiber type in AIS was largely restored by Schroth exercises. Conclusions FTO supports the formation of murine slow-twitch fibers in an NFATC1–YTHDF2 dependent manner. The consistent paraspinal muscle features seen in AIS and congenital scoliosis, as well as the reversible pattern of muscle fibers and expression of FTO in AIS suggest that FTO may contribute to the muscle fiber remodeling secondary to scoliosis.
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- 2023
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4. Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma
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Ming Luo, Mingyang Huang, Ningning Yang, Yufan Zhu, Peng Huang, Zhujun Xu, Wengang Wang, and Lin Cai
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Biology (General) ,QH301-705.5 ,Physiology ,QP1-981 - Abstract
Abstract Osteosarcoma (OS) is the most common primary malignant pediatric bone tumor and is characterized by high heterogeneity. Studies have revealed a wide range of phenotypic differences among OS cell lines in terms of their in vivo tumorigenicity and in vitro colony-forming abilities. However, the underlying molecular mechanism of these discrepancies remains unclear. The potential role of mechanotransduction in tumorigenicity is of particular interest. To this end, we tested the tumorigenicity and anoikis resistance of OS cell lines both in vitro and in vivo. We utilized a sphere culture model, a soft agar assay, and soft and rigid hydrogel surface culture models to investigate the function of rigidity sensing in the tumorigenicity of OS cells. Additionally, we quantified the expression of sensor proteins, including four kinases and seven cytoskeletal proteins, in OS cell lines. The upstream core transcription factors of rigidity-sensing proteins were further investigated. We detected anoikis resistance in transformed OS cells. The mechanosensing function of transformed OS cells was also impaired, with general downregulation of rigidity-sensing components. We identified toggling between normal and transformed growth based on the expression pattern of rigidity-sensing proteins in OS cells. We further uncovered a novel TP53 mutation (R156P) in transformed OS cells, which acquired gain of function to inhibit rigidity sensing, thus sustaining transformed growth. Our findings suggest a fundamental role of rigidity-sensing components in OS tumorigenicity as mechanotransduction elements through which cells can sense their physical microenvironment. In addition, the gain of function of mutant TP53 appears to serve as an executor for such malignant programs.
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- 2023
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5. Immune response to foreign materials in spinal fusion surgery
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Jia Cai, Wengang Wang, Peng Cai, and Bo Cao
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Spinal fusion ,Foreign body reaction ,Immune response ,Macrophages ,Bone growth ,Biocompatible materials ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Spinal fusion surgery is a common procedure used to stabilize the spine and treat back pain. The procedure involves the use of foreign materials such as screws, rods, or cages, which can trigger a foreign body reaction, an immune response that involves the activation of immune cells such as macrophages and lymphocytes. The foreign body reaction can impact the success of spinal fusion, as it can interfere with bone growth and fusion. This review article provides an overview of the cellular and molecular events in the foreign body reaction, the impact of the immune response on spinal fusion, and strategies to minimize its impact. By carefully considering the use of foreign materials and optimizing surgical techniques, the impact of the foreign body reaction can be reduced, leading to better outcomes for patients.
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- 2023
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6. Multi-supervised bidirectional fusion network for road-surface condition recognition
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Hongbin Zhang, Zhijie Li, Wengang Wang, Lang Hu, Jiayue Xu, Meng Yuan, Zelin Wang, Yafeng Ren, and Yiyuan Ye
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Automatic driving ,Road surface condition ,Multi-supervised bidirectional fusion network ,ConvNeXt ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Rapid developments in automatic driving technology have given rise to new experiences for passengers. Safety is a main priority in automatic driving. A strong familiarity with road-surface conditions during the day and night is essential to ensuring driving safety. Existing models used for recognizing road-surface conditions lack the required robustness and generalization abilities. Most studies only validated the performance of these models on daylight images. To address this problem, we propose a novel multi-supervised bidirectional fusion network (MBFN) model to detect weather-induced road-surface conditions on the path of automatic vehicles at both daytime and nighttime. We employed ConvNeXt to extract the basic features, which were further processed using a new bidirectional fusion module to create a fused feature. Then, the basic and fused features were concatenated to generate a refined feature with greater discriminative and generalization abilities. Finally, we designed a multi-supervised loss function to train the MBFN model based on the extracted features. Experiments were conducted using two public datasets. The results clearly demonstrated that the MBFN model could classify diverse road-surface conditions, such as dry, wet, and snowy conditions, with a satisfactory accuracy and outperform state-of-the-art baseline models. Notably, the proposed model has multiple variants that could also achieve competitive performances under different road conditions. The code for the MBFN model is shared at https://zenodo.org/badge/latestdoi/607014079.
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- 2023
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7. Endothelial cell-derived exosomes boost and maintain repair-related phenotypes of Schwann cells via miR199-5p to promote nerve regeneration
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Jinsheng Huang, Geyi Zhang, Senrui Li, Jiangnan Li, Wengang Wang, Jiajia Xue, Yuanyi Wang, Mengyuan Fang, and Nan Zhou
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Endothelial cell derived exosomes ,Schwann cell ,Repair-related cell phenotypes ,MiR199-5p ,Peripheral nerve regeneration ,Functional recovery ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Background Schwann cells (SCs) respond to nerve injury by transforming into the repair-related cell phenotype, which can provide the essential signals and spatial cues to promote axonal regeneration and induce target reinnervation. Endothelial cells (ECs) contribute to intraneural angiogenesis contributing to creating a permissive microenvironment. The coordination between ECs and SCs within injury sites is crucial in the regeneration process, however, it still unclear. As the intercellular vital information mediators in the nervous system, exosomes have been proposed to take a significant role in regulating regeneration. Thus, the main purpose of this study is to determine the facilitative effect of ECs-derived exosomes on SCs and to seek the underlying mechanism. Results In the present study, we collected exosomes from media of ECs. We demonstrated that exosomes derived from ECs possessed the favorable neuronal affinity both in vitro and in vivo. Further research indicated that EC-exosomes (EC-EXO) could boost and maintain repair-related phenotypes of SCs, thereby enhancing axonal regeneration, myelination of regenerated axons and neurologically functional recovery of the injured nerve. MiRNA sequencing in EXO-treated SCs and control SCs indicated that EC-EXO significantly up-regulated expression of miR199-5p. Furthermore, this study demonstrated that EC-EXO drove the conversion of SC phenotypes in a PI3K/AKT/PTEN-dependent manner. Conclusion In conclusion, our research indicates that the internalization of EC-EXO in SCs can promote nerve regeneration by boosting and maintaining the repair-related phenotypes of SCs. And the mechanism may be relevant to the up-regulated expression of miR199-5p and activation of PI3K/AKT/PTEN signaling pathway. Graphical Abstract
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- 2023
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8. Predicting potential miRNA-disease associations based on more reliable negative sample selection
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Ruiyu Guo, Hailin Chen, Wengang Wang, Guangsheng Wu, and Fangliang Lv
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miRNA-disease association predictions ,Supervised learning ,Negative sample selection ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Increasing biomedical studies have shown that the dysfunction of miRNAs is closely related with many human diseases. Identifying disease-associated miRNAs would contribute to the understanding of pathological mechanisms of diseases. Supervised learning-based computational methods have continuously been developed for miRNA-disease association predictions. Negative samples of experimentally-validated uncorrelated miRNA-disease pairs are required for these approaches, while they are not available due to lack of biomedical research interest. Existing methods mainly choose negative samples from the unlabelled ones randomly. Therefore, the selection of more reliable negative samples is of great importance for these methods to achieve satisfactory prediction results. Results In this study, we propose a computational method termed as KR-NSSM which integrates two semi-supervised algorithms to select more reliable negative samples for miRNA-disease association predictions. Our method uses a refined K-means algorithm for preliminary screening of likely negative and positive miRNA-disease samples. A Rocchio classification-based method is applied for further screening to receive more reliable negative and positive samples. We implement ablation tests in KR-NSSM and find that the combination of the two selection procedures would obtain more reliable negative samples for miRNA-disease association predictions. Comprehensive experiments based on fivefold cross-validations demonstrate improvements in prediction accuracy on six classic classifiers and five known miRNA-disease association prediction models when using negative samples chose by our method than by previous negative sample selection strategies. Moreover, 469 out of 1123 selected positive miRNA-disease associations by our method are confirmed by existing databases. Conclusions Our experiments show that KR-NSSM can screen out more reliable negative samples from the unlabelled ones, which greatly improves the performance of supervised machine learning methods in miRNA-disease association predictions. We expect that KR-NSSM would be a useful tool in negative sample selection in biomedical research.
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- 2022
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9. Evaluation of the operation status of the residential land market in Beijing-Tianjin-Hebei region of China and its spatiotemporal pattern characteristics.
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Xin Chen, Can Li, Huixia Li, Jingfeng Ge, Wengang Wang, and Pengfei An
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Medicine ,Science - Abstract
Exploring the operation status and patterns of urban land markets is an important theoretical and practical topic for promoting coordinated socio-economic development. In this study, the operation status of the residential land market in the Beijing-Tianjin-Hebei region and the characteristics of its pattern were analyzed using the composite index method and the 3σ rule of the normal distribution and taking the 174 counties in Beijing, Tianjin, and Hebei, China, as the research objects. The results show that ① Beijing, Tianjin, Langfang, Zhangjiakou, and Baoding residential land market state composite indexes are all in the middle to upper levels in the Beijing-Tianjin-Hebei city cluster, while Qinhuangdao, Handan, and Chengde residential land market state composite indexes are generally low. The harmony between the residential land price and national economy, the market supply and demand balance, and the structural balance may become the main factors affecting the healthy development of the residential land market in Beijing and Tianjin. ② The proportion of counties with "healthy" residential land market in all dimensions and overall market status reached over 64%, and the residential land market in the Beijing-Tianjin-Hebei region is running well. The rapid increase in residential land prices from 2016 to 2020 was an important driver of the increased heat in the residential land market across the region. ③ The residential land market in the counties around Beijing and Tianjin is significantly hotter than in other regions, and there is an obvious polarization effect in the operation state of the residential land market in the Beijing-Tianjin region. The residential land market is generally cold in the counties in the southern and northeastern parts of the region and other peripheral areas, and there is a risk of marginalization in the development of the residential land market in the counties in the peripheral areas. ④ Both the hot and cold residential land market states exhibit spatial clustering characteristics. Most of the clusters are not consistent with the municipal administrative boundaries, and the states of the residential land market in neighboring counties are very similar.
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- 2023
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10. Contribution Evaluation of Physical Hole Structure, Hydrogen Bond, and Electrostatic Attraction on Dye Adsorption through Individual Experiments
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Wengang Wang, Lei Wang, Ming Zhang, Bo Yu, Xiaoning Li, and Han Yan
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Physical and theoretical chemistry ,QD450-801 - Abstract
Disagreements over various unanswered questions about contribution of the adsorption process and functional groups on dye adsorption still exist. The main aim of this research was to evaluate the contributions of physical hole structure, hydrogen bond, and electrostatic attraction on dye adsorption. Three ideal representatives, namely, a sponge with porous structure, P(AM) containing -CONH2 groups, and P(AANa/AM) containing -COONa groups, were chosen to evaluate the above contributions. The methylene blue (MB) removal rates of these three products were compared through individual experiments. The results revealed that physical hole structure did not play a role in decreasing dye concentration. Hydrogen bond existed in dye adsorption but did not remarkably reduce dye concentration. The excellent removal results of P(AANa/AM) demonstrated that electrostatic attraction was critical in enriching dye contaminants from the solution into solid adsorbent. The results could provide insights into the dye adsorption mechanisms for further research.
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- 2023
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11. Research on the characteristics and influencing factors of the Beijing-Tianjin-Hebei urban network structure from the perspective of listed manufacturing enterprises.
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Wengang Wang, Chuning Miao, Haihang Yu, and Can Li
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Medicine ,Science - Abstract
The production connection is a crucial component of the Inter City Association. An urban network based on the enterprise perspective better reflects the structural characteristics of regional cities. Based on data gathered from the headquarters and branches of the listed manufacturing enterprises in 2020, this paper analyzes the county-level administrative units in the Beijing-Tianjin-Hebei region of China. Using the subordinate connection model and the social network analysis method, this paper examines the spatial structural characteristics and factors of urban networks in the Beijing-Tianjin-Hebei region. The results suggest that resource allocation in the Beijing-Tianjin-Hebei region is unbalanced, with a significant difference in urban radiation and agglomeration capacity. As the administrative centers, Beijing, Tianjin, and Shijiazhuang show a strong ability to allocate resources within the network. The overall network density in the region was shown to be relatively low, with the main links being of low or medium level. The urban network is defined by the network connection led by Beijing and Tianjin with Beijing-Tianjin-Tangshan and Beijing-Baoding-Shijiazhuang as the main axis. These cities exhibit a "dense southeast and sparse northwest" pattern. From a hierarchical perspective, high-level network connections are based mainly on spatial proximity. Analyzing the agglomerative subgroups, the study found that the inner and outer connections of the core subgroups were relatively high. Semi-marginal subgroups generally accepted the radiation of core subgroups, while marginal subgroups had little connection with other subgroups in the region. The results of the QAP analysis show that the administrative relationship, spatial distance, city size difference, economic development level difference, industrial structure difference, and labor cost difference have a significant influence on the urban network of the Beijing-Tianjin-Hebei region.
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- 2023
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12. Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1
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Na Kong, Xiaying Chen, Jiao Feng, Ting Duan, Shuiping Liu, Xueni Sun, Peng Chen, Ting Pan, Lili Yan, Ting Jin, Yu Xiang, Quan Gao, Chengyong Wen, Weirui Ma, Wencheng Liu, Mingming Zhang, Zuyi Yang, Wengang Wang, Ruonan Zhang, Bi Chen, Tian Xie, Xinbing Sui, and Wei Tao
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Baicalin ,Ferroptosis ,Bladder cancer ,FTH1 ,Deferoxamine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation. Currently, the therapeutic role of ferroptosis on cancer is gaining increasing interest. Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types; however, the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown. In the study, we investigated the effect of baicalin on bladder cancer cells 5637 and KU-19-19. As a result, we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells. Subsequently, we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo, accompanied by reactive oxygen species (ROS) accumulation and intracellular chelate iron enrichment. The ferroptosis inhibitor deferoxamine but not necrostatin-1, chloroquine (CQ), N-acetyl-l-cysteine, l-glutathione reduced, or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) rescued baicalin-induced cell death, indicating ferroptosis contributed to baicalin-induced cell death. Mechanistically, we show that ferritin heavy chain 1 (FTH1) was a key determinant for baicalin-induced ferroptosis. Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells. Taken together, our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis, which will hopefully provide a prospective compound for bladder cancer treatment.
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- 2021
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13. Preparation of ultrasound contrast agents: The exploration of the structure-echogenicity relationship of contrast agents based on neural network model
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Feng Li, Wensheng Xu, Yujin Feng, Wengang Wang, Hui Tian, Suhuan He, Liang Li, Bai Xiang, and Yueheng Wang
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ultrasound molecular imaging ,contrast-enhanced ultrasound ,ultrasound contrast agents ,preparation ,neural network model ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
There is a need to standardize the process of micro/nanobubble preparation to bring it closer to clinical translation. We explored a neural network-based model to predict the structure-echogenicity relationship for the preparation and fabrication of ultrasound-enhanced contrast agents. Seven formulations were screened, and 109 measurements were obtained. An artificial neural network-multilayer perceptron (ANN-MLP) model was used. The original data were divided into the training and testing groups, which included 73 and 36 groups of data, respectively. The hidden layer was selected from three hidden layers and included bias. The classification graph showed that the predicted values of the training and testing groups were 76.7% and 66.7%, respectively. According to the receiver operating characteristic curve, the accuracy of different imaging effects could achieve a prediction rate of 88.1–96.5%. The percentage graph showed that the data were gradually converging. The predictive analysis curves of different ultrasound effects gradually approached stable value of Gain. Normalized importance predicted contributions for the Pk1, poly-dispersity index (PDI), and intensity account were 100%, 98.5%, and 89.7%, respectively. The application of the ANN-MLP model is feasible and effective for the exploration of the synthesis process of ultrasound contrast agents. 1,2-Distearoyl-sn-glycero-3 phosphoethanolamine-N (methoxy[polyethylene glycol]-2000) (DSPE PEG-2000) correlated highly with the success rate of contrast agent synthesis.
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- 2022
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14. HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis
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Zheng Wang, Nan Zhou, Wengang Wang, Yangke Yu, Lei Xia, and Ning Li
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Osteoarthritis ,Histone deacetylase 2 ,MicroRNA-503-5p ,Serum- and glucocorticoid-inducible kinase-1 ,Chondrocyte ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Osteoarthritis (OA) is a disabling joint disease that causes articular cartilage degeneration. It has been implicated that altered expression of histone deacetylase 2 (HDAC2) is found in patients with OA. However, the specific role of HDAC2 in the development of OA still remains enigmatic. Hence, we sought to characterize the functional relevance of HDAC2 in the development of OA. Methods Anterior cruciate ligament surgery was performed to generate the rat model of OA. Luciferase assay was performed to evaluate the relationship between microRNA-503-5p (miR-503-5p) and serum- and glucocorticoid-inducible kinase-1 (SGK1). Functional experiments were conducted to examine the functional significance of miR-503-5p, histone deacetylase 2 (HDAC2), and SGK1 on the progression of OA by determining proliferation, apoptosis, and expression of apoptosis-associated proteins and inflammatory cytokines. Results HDAC2 could inhibit miR-503-5p expression. SGK1 was the target gene of miR-503-5p. Upregulation of miR-503-5p or silencing of HDAC2 contributed to enhanced proliferation, suppressed apoptosis (reduced expression of Caspase-3 and Bax but elevated expression of Bcl2), and promoted inflammation in chondrocytes of OA rats. Conclusion In conclusion, our study demonstrated that HDAC2 could promote OA through miR-503-5p/SGK1 axis, which might function as a therapeutic target for OA treatment.
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- 2021
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15. An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment
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Bingtao Zhai, Peng Chen, Wengang Wang, Shuiping Liu, Jiao Feng, Ting Duan, Yu Xiang, Ruonan Zhang, Mingming Zhang, Xuemeng Han, Xiaying Chen, Qiujie Li, Guohua Li, Ying Liu, Xingxing Huang, Wenzheng Zhang, Ting Pan, Lili Yan, Ting Jin, Tian Xie, and Xinbing Sui
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upar ,β-elemene ,active targeting liposome ,bladder cancer ,ddp ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Objective: In this study, we aimed to develop an amino-terminal fragment (ATF) peptide-targeted liposome carrying β-elemene (ATF24-PEG-Lipo-β-E) for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin (DDP) for bladder cancer treatment. Methods: The liposomes were prepared by ethanol injection and high-pressure microjet homogenization. The liposomes were characterized, and the drug content, entrapment efficiency, and in vitro release were studied. The targeting efficiency was investigated using confocal microscopy, ultra-fast liquid chromatography, and an orthotopic bladder cancer model. The effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8 (CCK-8) assay, a colony formation assay, and cell apoptosis and cell cycle analyses. The anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft model. Results: ATF24-PEG-Lipo-β-E had small and uniform sizes (˜79 nm), high drug loading capacity (˜5.24 mg/mL), high entrapment efficiency (98.37 ± 0.95%), and exhibited sustained drug release behavior. ATF24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol (PEG)ylated β-elemene liposomes (PEG-Lipo-β-E). DDP, combined with ATF24-PEG-Lipo-β-E, exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase, and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 pathways. Furthermore, the in vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors, using the combined strategy. Conclusions: The present study provided an effective strategy for the targeted delivery of β-elemene (β-E) to bladder cancer, and a combined strategy for bladder cancer treatment.
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- 2020
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16. Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes
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Bingtao Zhai, Qibiao Wu, Wengang Wang, Mingming Zhang, Xuemeng Han, Qiujie Li, Peng Chen, Xiaying Chen, Xingxing Huang, Guohua Li, Qin Zhang, Ruonan Zhang, Yu Xiang, Shuiping Liu, Ting Duan, Jianshu Lou, Tian Xie, and Xinbing Sui
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β-elemene ,pegylated liposome ,pharmacokinetics ,antitumor effect ,bladder cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Objective: This study aimed to develop a new polyethylene glycol (PEG)ylated β-elemene liposome (PEG-Lipo-β-E) and evaluate its characterization, pharmacokinetics, antitumor effects and safety in vitro and in vivo. Methods: The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization. Characterization of the liposomes was conducted, and drug content, entrapment efficiency (EE), in vitro release and stability were studied by ultra-fast liquid chromatography (UFLC) and a liquid surface method. Blood was drawn from rats to establish the pharmacokinetic parameters. The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model. Histological analyses were performed to evaluate safety. Results: The PEG-Lipo-β-E showed good stability and was characterized as 83.31 ± 0.181 nm in size, 0.279 ± 0.004 in polydispersity index (PDI), −21.4 ± 1.06 mV in zeta potential, 6.65 ± 0.02 in pH, 5.024 ± 0.107 mg/mL in β-elemene (β-E) content, and 95.53 ± 1.712% in average EE. The Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) indicated the formation of PEG-Lipo-β-E. Compared to elemene injection, PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance, a 1.62-fold increase in half-life, and a 1.76-fold increase in area under the concentration-time curves (AUCs) from 0 hour to 1.5 hours (P < 0.05). PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo. Histological analyses showed that there was no evidence of toxicity to the heart, kidney, liver, lung or spleen. Conclusions: The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation, high EE, good stability, improved bioavailability and antitumor effects.
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- 2020
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17. Natural Product Erianin Inhibits Bladder Cancer Cell Growth by Inducing Ferroptosis via NRF2 Inactivation
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Yu Xiang, Xiaying Chen, Wengang Wang, Lijuan Zhai, Xueni Sun, Jiao Feng, Ting Duan, Mingming Zhang, Ting Pan, Lili Yan, Ting Jin, Quan Gao, Chengyong Wen, Weirui Ma, Wencheng Liu, Deqiang Wang, Qibiao Wu, Tian Xie, and Xinbing Sui
- Subjects
Erianin ,ferroptosis ,bladder cancer ,nuclear factor E2-related factor 2 ,natural product ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Erianin, a natural product derived from Dendrobium chrysotoxum Lindl, has been proved to play antitumor activity in various cancers. However, the effects and molecular mechanisms of erianin in bladder cancer cells remain unexplored. In this study, we found that erianin triggered cell death and cell cycle arrest in bladder cancer cells. Then we demonstrated that erianin could promote the accumulation of lethal lipid-based reactive oxygen species (ROS) and the depletion of glutathione (GSH), suggesting the induction of ferroptosis. In the further study, the ferroptosis inhibitor deferoxamine (DFO), N-Acetylcysteine (NAC) and GSH but not necrostatin-1, CQ or Z-VAD-FMK rescued erianin-caused cell death, showing ferroptosis played a major role in erianin-caused cell death. In vivo, we also showed that erianin suppressed the tumor growth by inducing ferroptosis. Mechanistically, we demonstrated that nuclear factor E2-related factor 2 (NRF2) inactivation was a key determinant of ferroptosis caused by erianin. In bladder cancer cells, the compound tert-butylhydro-quinone (TBHQ), an activator of NRF2, suppressed erianin-induced ferroptosis. Whereas, NRF2 inhibition used shRNA augmented the ferroptosis response induced by erianin treatment. In conclusion, our data provide the first evidence that erianin can initiate ferroptosis-like cell death and lipid peroxidation in bladder cancer, which will hopefully become a promising anticancer compound for the treatment of bladder cancer.
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- 2021
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18. In vivo nuclear capture and molecular profiling identifies Gmeb1 as a transcriptional regulator essential for dopamine neuron function
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Luis M. Tuesta, Mohamed N. Djekidel, Renchao Chen, Falong Lu, Wengang Wang, Bernardo L. Sabatini, and Yi Zhang
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Science - Abstract
Despite the known role of midbrain dopaminergic (mDA) signaling in the homeostatic control of mood and motor functions, little is known about how gene expression in these neurons is regulated. Here, authors develop an in vivo nuclear tagging and capture technique for low-input chromatin accessibility and transcriptome profiling of genetically-defined neuron populations to identify Gmeb1 as a novel transcriptional regulator of mDA neurons, whose loss of function impairs motor control in mice.
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- 2019
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19. Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain
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Lynne Chantranupong, Jessica L Saulnier, Wengang Wang, Drew R Jones, Michael E Pacold, and Bernardo L Sabatini
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synaptic vesicle ,metabolomics ,neurotransmitters ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Neurons communicate by the activity-dependent release of small-molecule neurotransmitters packaged into synaptic vesicles (SVs). Although many molecules have been identified as neurotransmitters, technical limitations have precluded a full metabolomic analysis of SV content. Here, we present a workflow to rapidly isolate SVs and to interrogate their metabolic contents at high-resolution using mass spectrometry. We validated the enrichment of glutamate in SVs of primary cortical neurons using targeted polar metabolomics. Unbiased and extensive global profiling of SVs isolated from these neurons revealed that the only detectable polar metabolites they contain are the established neurotransmitters glutamate and GABA. In addition, we adapted the approach to enable quick capture of SVs directly from brain tissue and determined the neurotransmitter profiles of diverse brain regions in a cell-type-specific manner. The speed, robustness, and precision of this method to interrogate SV contents will facilitate novel insights into the chemical basis of neurotransmission.
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- 2020
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20. Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner
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Adam J Granger, Wengang Wang, Keiramarie Robertson, Mahmoud El-Rifai, Andrea F Zanello, Karina Bistrong, Arpiar Saunders, Brian W Chow, Vicente Nuñez, Miguel Turrero García, Corey C Harwell, Chenghua Gu, and Bernardo L Sabatini
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neuromodulation ,neurotransmitter co-release ,Acetylcholine ,GABA ,VIP ,cortical interneurons ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
The mouse cerebral cortex contains neurons that express choline acetyltransferase (ChAT) and are a potential local source of acetylcholine. However, the neurotransmitters released by cortical ChAT+ neurons and their synaptic connectivity are unknown. We show that the nearly all cortical ChAT+ neurons in mice are specialized VIP+ interneurons that release GABA strongly onto other inhibitory interneurons and acetylcholine sparsely onto layer 1 interneurons and other VIP+/ChAT+ interneurons. This differential transmission of ACh and GABA based on the postsynaptic target neuron is reflected in VIP+/ChAT+ interneuron pre-synaptic terminals, as quantitative molecular analysis shows that only a subset of these are specialized to release acetylcholine. In addition, we identify a separate, sparse population of non-VIP ChAT+ neurons in the medial prefrontal cortex with a distinct developmental origin that robustly release acetylcholine in layer 1. These results demonstrate both cortex-region heterogeneity in cortical ChAT+ interneurons and target-specific co-release of acetylcholine and GABA.
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- 2020
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21. β-Elemene Reverses the Resistance of p53-Deficient Colorectal Cancer Cells to 5-Fluorouracil by Inducing Pro-death Autophagy and Cyclin D3-Dependent Cycle Arrest
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Ruonan Zhang, Ting Pan, Yu Xiang, Mingming Zhang, Jiao Feng, Shuiping Liu, Ting Duan, Peng Chen, Bingtao Zhai, Xiaying Chen, Wengang Wang, Bi Chen, Xuemeng Han, Liuxi Chen, Lili Yan, Ting Jin, Ying Liu, Guohua Li, Xingxing Huang, Wenzheng Zhang, Yitian Sun, Qiujie Li, Qin Zhang, Lvjia Zhuo, Tian Xie, Qibiao Wu, and Xinbing Sui
- Subjects
colorectal cancer ,p53 ,β-elemene ,5-fluorouracil ,autophagy ,cell cycle ,Biotechnology ,TP248.13-248.65 - Abstract
ObjectiveColorectal cancer is a malignant tumor of the digestive system with high morbidity and mortality. 5-fluorouracil remains a widely used chemotherapeutic drug in the treatment of advanced colorectal cancer, but chemotherapy drugs are prone to develop drug resistance, p53 deletion or mutation is an important reason for the resistance of colorectal cancer cells to 5-fluorouracil. β-elemene has been proved to have the potential of reverse chemotherapy drug resistance, but the mechanism is unknown. This study aimed to investigate the effect of β-elemene to 5-fluorouracil in drug-resistant p53-deficient colorectal cancer cells HCT116p53–/–, and determine the possible molecular mechanism of β-elemene to reverse 5-fluorouracil resistance.MethodsThe effect of β-elemene on HCT116p53–/– cell activity was detected by Cell counting Kit-8. Cell proliferation was detected by monoclonal plate. The apoptosis was detected by flow cytometry and western blot. The autophagy was detected by western blot, immunofluorescence and transmission electron microscope. Determine the role of Cyclin-related protein Cyclin D3 in β-elemene reversing the resistance of HCT116p53–/– to 5-fluorouracil was detected by overexpression of Cyclin D3. The effect of β-elemene on the tumorigenic ability of p53-deficient colorectal cancer cells was detected establishing HCT116p53–/– all line xenograft model.ResultsFor p53 wildtype colorectal cancer cells, β-elemene could augment the sensitivity of 5-fluorouracil, for p53-deficient colorectal cancer cells, β-elemene significantly inhibited cell proliferation in a concentration-dependent manner, and reversed the resistance of HCT116p53–/– to 5-fluorouracil by inducing pro-death autophagy and Cyclin D3-dependent cycle arrest.Conclusionβ-elemene enhances the sensitivity of p53 wild-type cells to 5-fluorouracil, β-elemene can reverse the resistance of HCT116p53–/– to 5-fluorouracil by inducing pro-death autophagy and Cyclin D3-dependent cycle arrest in p53-deficient colorectal cancer, which will provide a new method for the treatment of p53 deletion colorectal cancer patients.
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- 2020
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22. Abnormal Striatal Development Underlies the Early Onset of Behavioral Deficits in Shank3B−/− Mice
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Rui Tiago Peixoto, Lynne Chantranupong, Richard Hakim, James Levasseur, Wengang Wang, Tasha Merchant, Kelly Gorman, Bogdan Budnik, and Bernardo Luis Sabatini
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Biology (General) ,QH301-705.5 - Abstract
Summary: The neural substrates and pathophysiological mechanisms underlying the onset of cognitive and motor deficits in autism spectrum disorders (ASDs) remain unclear. Mutations in ASD-associated SHANK3 in mice (Shank3B−/−) result in the accelerated maturation of corticostriatal circuits during the second and third postnatal weeks. Here, we show that during this period, there is extensive remodeling of the striatal synaptic proteome and a developmental switch in glutamatergic synaptic plasticity induced by cortical hyperactivity in striatal spiny projection neurons (SPNs). Behavioral abnormalities in Shank3B−/− mice emerge during this stage and are ameliorated by normalizing excitatory synapse connectivity in medial striatal regions by the downregulation of PKA activity. These results suggest that the abnormal postnatal development of striatal circuits is implicated in the onset of behavioral deficits in Shank3B−/− mice and that modulation of postsynaptic PKA activity can be used to regulate corticostriatal drive in developing SPNs of mouse models of ASDs and other neurodevelopmental disorders. : Peixoto et al. show that the onset of behavioral deficits in Shank3B−/− mice occurs during early postnatal development and that these can be ameliorated by reducing the glutamatergic synaptic drive in medial regions of the striatum by the downregulation of PKA activity. Keywords: corticostriatal, development, striatal, cortical, autism, Shank3, ASD, synaptic, plasticity, circuit
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- 2019
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23. Influence of screw density on thoracic kyphosis restoration in hypokyphotic adolescent idiopathic scoliosis
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Ming Luo, Honghui Jiang, Wengang Wang, Ning Li, Mingkui Shen, Peng Li, Genzhong Xu, and Lei Xia
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Adolescent idiopathic scoliosis ,Hypokyphosis ,Thoracic kyphosis ,Screw density ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Previous studies have reported that rod composition and diameter, as well as the correction technique are key factors associated with thoracic kyphosis (TK) restoration. However, few study has analyzed the correlation between screw density and TK restoration in hypokyphotic adolescent idiopathic scoliosis (AIS). Methods Fifty-seven thoracic AIS patients with preoperative TK
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- 2017
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24. Comparison of low density and high density pedicle screw instrumentation in Lenke 1 adolescent idiopathic scoliosis
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Mingkui Shen, Honghui Jiang, Ming Luo, Wengang Wang, Ning Li, Lulu Wang, and Lei Xia
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Adolescent idiopathic scoliosis ,Implant density ,Low density ,High density ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The correlation between implant density and deformity correction has not yet led to a precise conclusion in adolescent idiopathic scoliosis (AIS). The aim of this study was to evaluate the effects of low density (LD) and high density (HD) pedicle screw instrumentation in terms of the clinical, radiological and Scoliosis Research Society (SRS)-22 outcomes in Lenke 1 AIS. Methods We retrospectively reviewed 62 consecutive Lenke 1 AIS patients who underwent posterior spinal arthrodesis using all-pedicle screw instrumentation with a minimum follow-up of 24 months. The implant density was defined as the number of screws per spinal level fused. Patients were then divided into two groups according to the average implant density for the entire study. The LD group (n = 28) had fewer than 1.61 screws per level, while the HD group (n = 34) had more than 1.61 screws per level. The radiographs were analysed preoperatively, postoperatively and at final follow-up. The perioperative and SRS-22 outcomes were also assessed. Independent sample t tests were used between the two groups. Results Comparisons between the two groups showed no significant differences in the correction of the main thoracic curve and thoracic kyphosis, blood transfusion, hospital stay, and SRS-22 scores. Compared with the HD group, there was a decreased operating time (278.4 vs. 331.0 min, p = 0.004) and decreased blood loss (823.6 vs. 1010.9 ml, p = 0.048), pedicle screws needed (15.1 vs. 19.6, p
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- 2017
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25. Molecular targets of β-elemene, a herbal extract used in traditional Chinese medicine, and its potential role in cancer therapy: A review
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Bingtao Zhai, Nana Zhang, Xuemeng Han, Qiujie Li, Mingming Zhang, Xiaying Chen, Guohua Li, Ruonan Zhang, Peng Chen, Wengang Wang, Chenxi Li, Yu Xiang, Shuiping Liu, Ting Duan, Jianshu Lou, Tian Xie, and Xinbing Sui
- Subjects
Apoptosis ,β-Elemene ,Molecular targets ,Cancer ,Chemotherapy ,Multi-drug resistance ,Therapeutics. Pharmacology ,RM1-950 - Abstract
β-Elemene is a sesquiterpene compound extracted from the herb Curcuma Rhizoma and is used in traditional Chinese medicine (TCM) to treat several types of cancer, with no reported severe adverse effects. Recent studies, using in vitro and in vivo studies combined with molecular methods, have shown that β-elemene can inhibit cell proliferation, arrest the cell cycle, and induce cell apoptosis. Recent studies have identified the molecular targets of β-elemene that may have a role in cancer therapy. This review aims to discuss the anticancer potential of β-elemene through its actions on several molecular targets including kinase enzymes, transcription factors, growth factors and their receptors, and proteins. β-Elemene also regulates the expression of several key molecules that are involved in tumor angiogenesis and metastasis including vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs), E-cadherin, N-cadherin, and vimentin. Also, β-elemene has been shown to have regulatory effects on the immune response and increases the sensitivity of cancer cells to chemoradiotherapy and has shown effects on multidrug resistance (MDR) in malignancy. Recent studies have shown that β-elemene can induce autophagy, which prevents cancer cells from undergoing apoptosis. Therefore, the molecular mechanisms for the treatment effects on cancer of the herbal extract, β-elemene, which has been used for centuries in traditional Chinese medicine, are now being studied and identified.
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- 2019
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26. The Inhibition of RANKL-Induced Osteoclastogenesis through the Suppression of p38 Signaling Pathway by Naringenin and Attenuation of Titanium-Particle-Induced Osteolysis
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Wengang Wang, Chuanlong Wu, Bo Tian, Xuqiang Liu, Zanjing Zhai, Xinhua Qu, Chuan Jiang, Zhengxiao Ouyang, Yuanqing Mao, Tingting Tang, An Qin, and Zhenan Zhu
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naringenin ,osteolysis ,osteoclast ,p38 signaling ,NFATc1 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The aim of this study was to assess the effect of naringenin on osteoclastogenesis and titanium particle-induced osteolysis. Osteolysis from wear-induced particles and aseptic loosening are the most frequent late complications of total joint arthroplasty leading to revision of the prosthesis. Osteolysis during aseptic loosening is most likely due to increased bone resorption by osteoclasts. Through in vitro studies, we demonstrated that naringenin, a naturally occurring flavanone in grapefruit and tomatoes, exerts potent inhibitory effects on the ligand of the receptor activator of nuclear factor-κB (RANKL)-induced osteoclastogenesis and revealed that the mechanism of action of naringenin, which inhibited osteoclastogenesis by suppression of the p38 signaling pathway. Through in vivo studies, we proved that naringenin attenuated titanium particle-induced osteolysis in a mouse calvarial model. In general, we demonstrated that naringenin inhibited osteoclastogenesis via suppression of p38 signaling in vitro and attenuated titanium particle-induced osteolysis in vivo. This study also suggested that naringenin has significant potential for the treatment of osteolysis-related diseases caused by excessive osteoclast formation and activity.
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- 2014
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27. Neuromodulation of excitatory synaptogenesis in striatal development
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Yevgenia Kozorovitskiy, Rui Peixoto, Wengang Wang, Arpiar Saunders, and Bernardo L Sabatini
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basal ganglia ,circuit development ,corticostriatal ,dopamine ,pka ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Dopamine is released in the striatum during development and impacts the activity of Protein Kinase A (PKA) in striatal spiny projection neurons (SPNs). We examined whether dopaminergic neuromodulation regulates activity-dependent glutamatergic synapse formation in the developing striatum. Systemic in vivo treatment with Gαs-coupled G-protein receptors (GPCRs) agonists enhanced excitatory synapses on direct pathway striatal spiny projection neurons (dSPNs), whereas rapid production of excitatory synapses on indirect pathway neurons (iSPNs) required the activation of Gαs GPCRs in SPNs of both pathways. Nevertheless, in vitro Gαs activation was sufficient to enhance spinogenesis induced by glutamate photolysis in both dSPNs and iSPNs, suggesting that iSPNs in intact neural circuits have additional requirements for rapid synaptic development. We evaluated the in vivo effects of enhanced glutamate release from corticostriatal axons and postsynaptic PKA and discovered a mechanism of developmental plasticity wherein rapid synaptogenesis is promoted by the coordinated actions of glutamate and postsynaptic Gαs-coupled receptors.
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- 2015
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28. ClKI: closed-loop and knowledge iterative via self-distillation for image sentiment analysis.
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Hongbin Zhang 0004, Meng Yuan, Lang Hu, Wengang Wang, Zhijie Li, Yiyuan Ye, Yafeng Ren, and Donghong Ji
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- 2024
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29. Predicting miRNA-disease associations based on lncRNA-miRNA interactions and graph convolution networks.
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Wengang Wang and Hailin Chen
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- 2023
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30. Biostatistics of VHL-Gene Transfection in the Health Informatics Analysis of Renal Cell Carcinoma.
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Yunxiang Gong, Degang Wang, and Wengang Wang
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- 2022
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31. Predicting miRNA-disease associations based on graph attention networks and dual Laplacian regularized least squares.
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Wengang Wang and Hailin Chen
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- 2022
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32. Soaking pretreatment of hydrogel adsorbent in distilled water to enhance efficiency of adsorption onto low concentration dye
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Wengang Wang, Qi Wu, Lei Wang, Ming Zhang, Bo Yu, Xiaoning Li, Han Yan, and Li Wan
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Polymers and Plastics ,Materials Chemistry ,General Chemistry - Published
- 2023
33. Neuronal-Activity Dependent Mechanisms of Small Cell Lung Cancer Progression
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Solomiia Savchuk, Kaylee Gentry, Wengang Wang, Elana Carleton, Belgin Yalçın, Yin Liu, Elisa C. Pavarino, Jenna LaBelle, Angus M. Toland, Pamelyn J. Woo, Fangfei Qu, Mariella G. Filbin, Mark A. Krasnow, Bernardo L. Sabatini, Julien Sage, Michelle Monje, and Humsa S. Venkatesh
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Article - Abstract
SummaryNeural activity is increasingly recognized as a critical regulator of cancer growth. In the brain, neuronal activity robustly influences glioma growth both through paracrine mechanisms and through electrochemical integration of malignant cells into neural circuitry via neuron-to-glioma synapses, while perisynaptic neurotransmitter signaling drives breast cancer brain metastasis growth. Outside of the CNS, innervation of tumors such as prostate, breast, pancreatic and gastrointestinal cancers by peripheral nerves similarly regulates cancer progression. However, the extent to which the nervous system regulates lung cancer progression, either in the lung or when metastatic to brain, is largely unexplored. Small cell lung cancer (SCLC) is a lethal high-grade neuroendocrine tumor that exhibits a strong propensity to metastasize to the brain. Here we demonstrate that, similar to glioma, metastatic SCLC cells in the brain co-opt neuronal activity-regulated mechanisms to stimulate growth and progression. Optogenetic stimulation of cortical neuronal activity drives proliferation and invasion of SCLC brain metastases. In the brain, SCLC cells exhibit electrical currents and consequent calcium transients in response to neuronal activity, and direct SCLC cell membrane depolarization is sufficient to promote the growth of SCLC tumors. In the lung, vagus nerve transection markedly inhibits primary lung tumor formation, progression and metastasis, highlighting a critical role for innervation in overall SCLC initiation and progression. Taken together, these studies illustrate that neuronal activity plays a crucial role in dictating SCLC pathogenesis in both primary and metastatic sites.
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- 2023
34. Predicting miRNA-disease associations based on lncRNA–miRNA interactions and graph convolution networks
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Wengang Wang and Hailin Chen
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Molecular Biology ,Information Systems - Abstract
Increasing studies have proved that microRNAs (miRNAs) are critical biomarkers in the development of human complex diseases. Identifying disease-related miRNAs is beneficial to disease prevention, diagnosis and remedy. Based on the assumption that similar miRNAs tend to associate with similar diseases, various computational methods have been developed to predict novel miRNA-disease associations (MDAs). However, selecting proper features for similarity calculation is a challenging task because of data deficiencies in biomedical science. In this study, we propose a deep learning-based computational method named MAGCN to predict potential MDAs without using any similarity measurements. Our method predicts novel MDAs based on known lncRNA–miRNA interactions via graph convolution networks with multichannel attention mechanism and convolutional neural network combiner. Extensive experiments show that the average area under the receiver operating characteristic values obtained by our method under 2-fold, 5-fold and 10-fold cross-validations are 0.8994, 0.9032 and 0.9044, respectively. When compared with five state-of-the-art methods, MAGCN shows improvement in terms of prediction accuracy. In addition, we conduct case studies on three diseases to discover their related miRNAs, and find that all the top 50 predictions for all the three diseases have been supported by established databases. The comprehensive results demonstrate that our method is a reliable tool in detecting new disease-related miRNAs.
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- 2022
35. Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1
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Ting Jin, Shuiping Liu, Wengang Wang, Bi Chen, Quan Gao, Xiaying Chen, Ruonan Zhang, Ting Pan, Mingming Zhang, Weirui Ma, Xueni Sun, Xinbing Sui, Ting Duan, Yu Xiang, Lili Yan, Jiao Feng, Wencheng Liu, Chengyong Wen, Peng Chen, Tian Xie, Zuyi Yang, Na Kong, and Wei Tao
- Subjects
Programmed cell death ,Short Communication ,RM1-950 ,Deferoxamine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Ferroptosis ,Baicalin ,General Pharmacology, Toxicology and Pharmaceutics ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,biology ,Bladder cancer ,Cancer ,biology.organism_classification ,medicine.disease ,Baicalein ,FTH1 ,chemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Scutellaria baicalensis ,Therapeutics. Pharmacology ,Intracellular - Abstract
Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation. Currently, the therapeutic role of ferroptosis on cancer is gaining increasing interest. Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types; however, the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown. In the study, we investigated the effect of baicalin on bladder cancer cells 5637 and KU-19-19. As a result, we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells. Subsequently, we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo, accompanied by reactive oxygen species (ROS) accumulation and intracellular chelate iron enrichment. The ferroptosis inhibitor deferoxamine but not necrostatin-1, chloroquine (CQ), N-acetyl-l-cysteine, l-glutathione reduced, or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) rescued baicalin-induced cell death, indicating ferroptosis contributed to baicalin-induced cell death. Mechanistically, we show that ferritin heavy chain 1 (FTH1) was a key determinant for baicalin-induced ferroptosis. Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells. Taken together, our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis, which will hopefully provide a prospective compound for bladder cancer treatment., Graphical abstract Baicalin exerts its anticancer activity in bladder cancer by inducing ferritin heavy chain 1 (FTH1)-dependent ferroptosis, which will hopefully provide great therapeutic potential for bladder cancer treatment.Image 1
- Published
- 2021
36. An Online Calculator for the Prediction of 5-year Survival in Patients with Non-Metastatic Chondrosarcoma Using Machine Learning
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Tailong Chen, Zhongxin Tang, Xudong Wang, Wengang Wang, and Nan Zhou
- Abstract
Background: Most prior studieshave used conventional statistical techniquessuch asthe nomogram to examine patient prognosis and survival based on the distinct subtypes and primary locations of chondrosarcoma. Nevertheless, few studies have examined overall survival in patients with non-metastatic chondrosarcomas. We aim to develop a machine learning algorithm to evaluate 5-year survival in this cohort and transform the most accurate prediction model into an online calculator for clinical use. Methods: Between 1975 and 2018, we gathered data on patients with non-metastatic chondrosarcoma from the Surveillance, Epidemiological, and End Result databases. From these data, nine features were used to develop four machine learning models, including the boosted decision tree, support vector machine, bayes point machine, and neural network models, which were then evaluated and compared based on discrimination, calibration, and overall performance. Results: Ultimately, 1202 patients met our inclusion criteria. With a c statistic of 0.88, a calibration slope of 0.997, a calibration intercept of -0.02, and a Brier score of 0.12, the Bayes point machine performed best overall, and it was integrated into a free, publicly available software interface that can be found at https://bayesglm.shinyapps.io/non-metastatic-chondrosarcoma/. Conclusion: Although this Bayes point machine-based prediction model has not been externally validated, the online calculator can be used as a reference tool for medical staff as well as patients on survival prediction. External validation of this prediction model should be the focus of future research to improve its credibility.
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- 2022
37. Local and long-distance inputs dynamically regulate striatal acetylcholine during decision making
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Lynne Chantranupong, Celia C. Beron, Joshua A. Zimmer, Michelle J. Wen, Wengang Wang, and Bernardo L. Sabatini
- Abstract
Within the basal ganglia, striatal dopamine (DA) and acetylcholine (Ach) are essential for the selection and reinforcement of motor actions and decision making. In vitro studies have revealed a circuit local to the striatum by which each of these two neurotransmitters directly regulates release of the other. Ach, released by a unique population of cholinergic interneurons (CINs), drives DA release via direct axonal depolarization. In turn, DA inhibits CIN activity via dopamine D2 receptors (D2R). Whether and how this circuit contributes to striatal function in vivo remains unknown. To define the in vivo role of this circuit, we monitored Ach and DA signals in the ventrolateral striatum of mice performing a reward-based decision-making task. We establish that DA and Ach exhibit multiphasic and anticorrelated transients that are modulated by decision history and reward outcome. However, CIN perturbations reveal that DA dynamics and reward-prediction error encoding do not require Ach release by CINs. On the other hand, CIN-specific deletion of D2Rs shows that DA inhibits Ach levels in a D2R-dependent manner, and loss of this regulation impairs decision-making. To determine how other inputs to striatum shape Ach signals, we assessed the contribution of projections from cortex and thalamus and found that glutamate release from both sources is required for Ach release. Altogether, we uncover a dynamic relationship between DA and Ach during decision making and reveal modes of CIN regulation by local DA signals and long-range cortical and thalamic inputs. These findings deepen our understanding of the neurochemical basis of decision making and behavior.
- Published
- 2022
38. Efficacy and safety of Biqi capsule in the treatment of knee osteoarthritis: A Protocol of a randomized controlled trial
- Author
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Wengang Wang, Mengrui Jia, YaZhou Zhou, Hui Huang, and Ke Tian
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,WOMAC ,business.industry ,Standard treatment ,Capsule ,General Medicine ,Osteoarthritis ,Diclofenac Sodium ,medicine.disease ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Knee pain ,Randomized controlled trial ,law ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,030212 general & internal medicine ,medicine.symptom ,business ,Cartilage degeneration - Abstract
Background: Knee osteoarthritis (KOA) is a chronic and degenerative bone and joint disease, with knee osteoarthritis, cartilage degeneration, destruction and subchondral bone remodeling as the main pathological features. Its clinical symptoms are knee pain, swelling, limited activity, and long course of disease can cause joint deformities. At present, the early treatment of western medicine is mainly the use of non-steroidal drugs for anti-inflammation and removing pain, but because the efficacy of these drugs is unstable, the disease is easy to repeat after treatment, and the clinical effect is not good .Although Biqi capsule has advantages in the treatment of knee osteoarthritis, there is a lack of standard clinical studies to verify it, so the purpose of this randomized controlled study is to evaluate the efficacy and safety of Biqi capsule in the treatment of knee osteoarthritis. Methods: This is a prospective randomized controlled trial to study the efficacy and safety of Biqi capsule in the treatment of knee osteoarthritis. The patients were randomly divided into a treatment group and a control group according to 1:1. Among them, treatment group: Biqi capsule combined with diclofenac sodium sustained release tablets; Control group: Diclofenac sodium sustained-release tablets alone. Both groups were treated with standard treatment for 2 weeks and were followed up for 30 days to pay attention to the efficacy and safety indexes. Observation indicators included: the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Hospital for Special Surgery Knee Score (HSS), liver and kidney function, adverse reactions and so on. SPSS 25.0 software is used for data analysis. Discussion: This study will evaluate the efficacy and safety of Biqi capsule in the treatment of knee osteoarthritis, and the results of this experiment will provide a clinical basis for Biqi capsule in the treatment of knee osteoarthritis.
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- 2022
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39. Endothelial cell-derived exosomes promote nerve regeneration by boosting and maintaining repair-related phenotypes in Schwann cells
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Jinsheng Huang, Geyi Zhang, Senrui Li, Tailong Chen, Wengang Wang, Fei Chen, Mengyuan Fang, and Nan Zhou
- Abstract
Background: Schwann cells respond to nerve injury by transforming into a cell phenotype that promotes repair. These repair Schwann cells provide the essential signals and spatial cues to encourage axonal regeneration and induce target reinnervation. Endothelial cells (ECs), another crucial component of nerve tissue, contribute to the formation of regenerative microenvironment. Therefore, the way to control these peculiar cells, amplify their supportive functions and prevent their deterioration could be remarkable for the treatment of injured peripheral nerves. As the vital information mediators in the nervous system, exosomes have been proposed to take a significant role in regulating regeneration.Results: In the present study, we collected exosomes from media of ECs. We demonstrated that exosomes derived from ECs possessed the favorable neuronal affinity both in vitro and in vivo. Further research indicated that EC-exosomes could boost and maintain repair-related phenotypes of Schwann cells, thereby enhancing axonal regeneration, myelination of regenerated axons and neurologically functional recovery of the injured nerve. We found some highly enriched miRNAs in EC-exosomes, including let-7f-5p, miR199-5p and miR-3074-5p by the sequencing of miRNAs. In addition, miRNA sequencing in EXO-treated Schwann cells and control Schwann cells indicated that EC-exosomes significantly up-regulated the expression of miR199-5p. Furthermore, this study demonstrated that EC-exosomes drove the conversion of Schwann cell phenotypes in a PI3K/AKT/PTEN-dependent manner. Conclusion: In conclusion, our research indicates that the internalization of EC-exosomes in Schwann cells can promote nerve regeneration by boosting and maintaining the repair-related phenotypes of Schwann cells. And the mechanism may be relevant to the regulation of miRNAs and activation of PI3K/AKT/PTEN signaling pathway.
- Published
- 2022
40. Research on Rolling Bearing Operating Life Based on Principal Component Analysis
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Wengang Wang, Xu Hao, Yingchao Wang, and Yu Yuan
- Subjects
History ,Computer Science Applications ,Education - Abstract
In order to analyze the running state of rolling bearing, the principle component analysis is used to extract new parameters to analyze the running state of the rolling bearing. Firstly, the whole life cycle vibration signal of the rolling bearing is collected by using an acceleration sensor. Secondly, Empirical Mode Decomposition (EMD) is used to reduce the noise in the vibration signal. Then, the main components of the time domain parameters are extracted using the principle component of the denoised signal. And the main components are synthesized into new parameter indicators. Finally, under the new parameter index, the envelope analysis is used to analyze the signal classification. The results show that the new parameter index can divide the entire life cycle of the rolling bearing into four different operating periods, each representing a different bearing operating state.
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- 2023
41. Biomaterials for Repairing Gaps After Peripheral Nerve Injury
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Dong-Xu Huang, Nan Zhou, Wengang Wang, Lei Xia, Jiangnan Li, Ge-Yi Zhang, and Xu Gong
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Pathology ,medicine.medical_specialty ,Materials science ,Peripheral nerve injury ,medicine ,General Materials Science - Abstract
Peripheral nerves have complex and precise structures that differ from other types of tissues and intrinsic regeneration abilities after injury. Spontaneous recovery is possible for neuropraxia and axonotmesis, while surgical treatment is required for neurotmesis. It remains a challenge to repair nerve gaps, a series of severe neurotmesis. It seems that 3 cm is the upper limit distance for primate peripheral nerves to regenerate spontaneously. Nerve autografts are the gold standard treatment for bridging nerve gaps. In the present review, current biomaterials for repairing gaps after peripheral nerve injury are briefly summarized. Moreover, the microstructure of the peripheral nerve, classifications of peripheral nerve injury, and the Wallerian degeneration are reviewed in the biological view and clinical practice. The failure of nerve regeneration in nerve conduits bridging longer than 3 cm gaps may be contributing to the insufficient vascularization of nerve conduit materials. Future researchers could focus on advanced biomaterials that promoting the angiogenesis of nerve conduits.
- Published
- 2021
42. Enhanced Transrectal Ultrasound, Real-Time Sonoelastography, and Contrast-Enhanced Transrectal Ultrasound in Heavily Prescreened Chinese Men With Naive and Repetitive Biopsy: A Comparison of Detection Rate of Prostate Cancer Per Man and Per Lesion
- Author
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Yi Wang, Yujin Feng, Xiaoyun Yang, Wengang Wang, Tongdi Zhang, Yingxin Xie, and Kun Zhao
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Image-Guided Biopsy ,Male ,Biopsy ,Prostate ,General Earth and Planetary Sciences ,Elasticity Imaging Techniques ,Humans ,Prostatic Neoplasms ,Magnetic Resonance Imaging ,General Environmental Science ,Retrospective Studies - Abstract
Multiparametric magnetic resonance imaging and targeted biopsy have been widely accepted as the most accurate technique to detect localize prostate cancer. It is a time-consuming and expensive option and may not be widely available in China, making ultrasound the first choice for the detection of prostate cancer. In this current retrospective study, the diagnostic values of enhanced transrectal ultrasound, contrast-enhanced transrectal ultrasound, and real-time sonoelastography were evaluated. Symptomatic 315 men older than 40 years with prostate-specific antigen level greater than 4.0 ng/mL, with abnormal digital rectal examinations, and with suspicious lesions for prostate cancer under enhanced transrectal ultrasound included in the study. Enhanced transrectal ultrasound was suspicious in all 315 men, with 189 of 315 men with prostate cancer according to the prostate biopsy report. Sonoelastography was suspicious in 294 of 315 men, with 166 of 315 men with prostate cancer according to the prostate biopsy report. Contrast-enhanced transrectal ultrasound was suspicious in 221 of 315 men, with 159 of 315 men with prostate cancer according to the prostate biopsy report. Real-time sonoelastography alone and contrast-enhanced transrectal ultrasound alone were missed in 27 (11%) and 39 (15%) lesions to report cancer through biopsies. Working area for enhanced transrectal ultrasound, real-time sonoelastography, and contrast-enhanced transrectal ultrasound for detection of prostate cancer were 0 to 1 diagnostic confidence, 0.11 to 0.895 diagnostic confidence, and 0.39 to 0.63 diagnostic confidence respectively. Real-time sonoelastography and contrast-enhanced transrectal ultrasound may improve the detection of prostate cancer in men with suspicious prostate lesions under enhanced transrectal ultrasound (Level of Evidence: III; Technical Efficacy Stage: 2).
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- 2022
43. Morphological Differences Between the Pedicles in Nondystrophic Scoliosis Secondary to Neurofibromatosis Type 1 and Those in Adolescent Idiopathic Scoliosis
- Author
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Ming Luo, Ningning Yang, Lei Xia, Wengang Wang, and Shixin Zhao
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Male ,Neurofibromatosis 1 ,Adolescent ,Perforation (oil well) ,Computed tomography ,Idiopathic scoliosis ,Scoliosis ,Neurosurgical Procedures ,Thoracic Vertebrae ,03 medical and health sciences ,0302 clinical medicine ,Pedicle Screws ,medicine ,Humans ,In patient ,Neurofibromatosis ,Child ,Pedicle screw ,Retrospective Studies ,Medical Errors ,medicine.diagnostic_test ,business.industry ,Incidence ,medicine.disease ,030220 oncology & carcinogenesis ,Female ,Surgery ,Neurology (clinical) ,Tomography, X-Ray Computed ,business ,Nuclear medicine ,030217 neurology & neurosurgery - Abstract
Objective To compare the pedicle morphology of nondystrophic scoliosis secondary to neurofibromatosis type 1 (ND-NF1) scoliosis secondary to neurofibromatosis type 1 (NF1-S) and adolescent idiopathic scoliosis (AIS) using computed tomography (CT) in a consistent classification system. Methods The transverse pedicle widths of cancellous and cortical channels were measured using preoperative spinal CT images (T1–L5) and then classified as having type A–E pedicles; type B, C, D, and E pedicles were regarded as abnormal. A subset of these patients, a postoperative CT was performed to evaluate the accuracy of pedicle screw placement. According to the perforation of the medial or lateral pedicle walls by the pedicle screw, the placement of screw was classified by a grade ranging from 0 to 3, and grades 2 and 3 were regarded as misplacement. Results In total, 3230 pedicles were measured and classified, and the incidence rate of abnormal pedicles in the ND-NF1 group was significantly greater than that of the AIS group (65.9% vs. 61.4%, P = 0.03). The misplacement rate in the ND-NF1 group was significantly greater than that in the AIS group (12.2% vs. 7.4%; P = 0.01), and in terms of the corresponding 1100 pedicles, the incidence rate of abnormal pedicles in the ND-NF1 group was higher than that in the AIS group (71.7% vs. 64.9%; P = 0.02). Conclusions The incidence rate of abnormal pedicles in patients with ND-NF1 is significantly greater than that of patients with AIS, and it may the reason for the higher misplacement rate of pedicle screws in patients with ND-NF1.
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- 2020
44. Diagnosis of Atherosclerotic Plaques Using Vascular Endothelial Growth Factor Receptor-2 Targeting Antibody Nano-microbubble as Ultrasound Contrast Agent
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Yi Wang, Yujin Feng, Xiaoyun Yang, Wengang Wang, and Yueheng Wang
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Vascular Endothelial Growth Factor A ,Microbubbles ,Neovascularization, Pathologic ,General Immunology and Microbiology ,Article Subject ,Applied Mathematics ,technology, industry, and agriculture ,Contrast Media ,Endothelial Cells ,General Medicine ,Vascular Endothelial Growth Factor Receptor-2 ,Antibodies ,Plaque, Atherosclerotic ,General Biochemistry, Genetics and Molecular Biology ,Modeling and Simulation ,Humans ,Ultrasonography - Abstract
The atherosclerotic plaque is characterized by narrowing of blood vessels and reduced blood flow leading to the insufficient blood supply to the brain. The hemodynamic changes caused by arterial stenosis increase the shearing force of the fibrous cap on the surface of the plaque, thereby reducing the stability of the plaque. Unstable plaques are more likely to promote angiogenesis and increase the risk of patients with cerebrovascular diseases. A timely understanding of the formation and stability of the arterial plaque can guide in taking targeted measures for reducing the risk of acute stroke in patients. It has been confirmed that nano-microbubbles can enter these plaques through the gaps in the patient’s vascular endothelial cells, thereby enhancing the acquisition of ultrasound information for plaque visualization. Therefore, we aim to investigate the diagnostic value of targeted nano-microbubbles for atherosclerotic plaques. This study constructed vascular endothelial growth factor receptor-2 (VEGFR-2) targeting antibody nano-microbubbles and compared its diagnostic value with that of blank nano-microbubbles for atherosclerotic plaques. Studies have found that VEGFR-2 targeting antibody nano-microbubbles can accurately detect the position of plaques. Its detection rate, sensitivity, and specificity for plaques are higher than those of blank nano-microbubbles. Similarly, the peak intensity and average transit time of VEGFR-2 targeting antibody nano-microbubbles were greater than those of blank nano-microbubbles. Therefore, we believe that the combination of VEGFR-2 antibody and nano-microbubbles can enhance the acquisition of ultrasound information on atherosclerotic plaque neovascularization, thereby improving the early diagnosis of unstable plaque.
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- 2022
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45. Evaluation of Achilles Tendon in Patients with Diabetic Foot Ulcer by High-Frequency Ultrasound Combined with Real-Time Shear-Wave Elastography
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Fan Zhang, Juan Wang, Yulin Ma, Yi Wang, Wengang Wang, and Yueheng Wang
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Medicine (General) ,Article Subject ,Biomedical Engineering ,Health Informatics ,Achilles Tendon ,Diabetic Foot ,R5-920 ,Diabetes Mellitus ,Medical technology ,Elasticity Imaging Techniques ,Humans ,Surgery ,R855-855.5 ,Retrospective Studies ,Ultrasonography ,Research Article ,Biotechnology - Abstract
To our best knowledge, no study investigated the utility of the combination of high-frequency US and real-time shear wave elastography (RTSWE) in the evaluation of Achilles tendon (AT). Our study aimed to evaluate AT in patients with diabetic foot ulcers using high-frequency US and RTSWE. We retrospectively reviewed 100 patients who visited our hospital due to diabetes. Patients were divided into the study group (those with diabetic foot ulcers) and the control group (those without diabetic foot ulcers). Patients’ demographics, basic medical records, and laboratory tests were reviewed. High-frequency ultrasound and RTSWE were performed in both AT for all 100 patients. Young’s modulus was measured at the upper, middle, and lower parts of each AT by RTSWE. There were 50 patients with diabetic foot ulcers. Patients in the study group had older age, a higher incidence of insulin use, and a higher level of cholesterol than those in the control group. However, thickness and Young’s modulus of AT were comparable in two groups, when evaluated by the combination of high-frequency ultrasound and RTSWE. There was no significant difference in thickness and elastic modulus of AT in patients with and without diabetic foot ulcers when evaluated by the combination of high-frequency ultrasound and RTSWE.
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- 2022
- Full Text
- View/download PDF
46. Predictive value of single-nucleotide polymorphisms in curve progression of adolescent idiopathic scoliosis
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Wengang Wang, Tailong Chen, Yibin Liu, Songsong Wang, Ningning Yang, and Ming Luo
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Adolescent ,Asian People ,Genotype ,Scoliosis ,Case-Control Studies ,Humans ,Cytokines ,Reproducibility of Results ,Orthopedics and Sports Medicine ,Surgery ,Genetic Predisposition to Disease ,Kyphosis ,Polymorphism, Single Nucleotide - Abstract
Genetic diagnosis is a promising approach because several single-nucleotide polymorphisms (SNPs) associated with adolescent idiopathic scoliosis (AIS) progression have been reported. We review the predictive value of SNPs in curve progression of adolescent idiopathic scoliosis.We reviewed DNA-based prognostic testing to predict curve progression. Then, the multiple polymorphisms in loci related to AIS progression were also reviewed, and we elucidated the predictive value of SNPs from four functional perspectives, including endocrine metabolism, neuromuscular system, cartilage and extracellular matrix, enzymes, and cytokines.The ScoliScores were less successful predictors than expected, and the weak power of predictive SNPs might account for its failure. Susceptibility loci in ESR1, ESR2, GPER, and IGF1, which related to endocrine metabolism, have been reported to predict AIS progression. Neuromuscular imbalance might be a potential mechanism of scoliosis, and SNPs in LBX1, NTF3, and SOCS3 have been reported to predict the curve progression of AIS. Susceptibility loci in SOX9, MATN1, AJAP1, MMP9, and TIMP2, which are related to cartilage and extracellular matrix, are also potentially related to AIS progression. Enzymes and cytokines play essential roles in regulating bone metabolism and embryonic development. SNPs in BNC2, SLC39A8, TGFB1, IL-6, IL-17RC, and CHD7 were suggested as predictive loci for AIS curve progression.Many promising SNPs have been identified to predict the curve progression of AIS. However, conflicting results from replication studies and different ethnic groups hamper their reliability. Convincing SNPs from multiethnic populations and functional verification are needed.
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- 2021
47. Study on Mountain Space in Mount Tai Region Based on GIS Spatial Data Analysis
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Congcong Liu, Fei Wang, Wengang Wang, Qingliang Zhang, and Lingfang Zhang
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- 2021
48. EXPERIMENTAL STUDY ON PORE STRUCTURE AND OIL/WATER TWO-PHASE FLOW CHARACTERISTICS OF SANDSTONE RESERVOIR.
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Xiujuan Wang, Dongxia Chen, Jinhua Fu, Shaochen Jin, Zhongyi Xu, Wengang Wang, Wei Zhang, Shuai Yang, and Dongliang Zhang
- Abstract
The fine characterization of the micro pore structure of low-permeability sandstone has become the key technology for the efficient development of this kind of reservoir. In this study, the micro fine modeling of three-dimensional pore structure is established through the micro-CT scanning, and quantitative parameter characterization analysis is carried out combined with the image processing technology. Based on this, the digital rock electrical experiments of gas/water two-phase saturated low permeability sandstone is carried out, and the influence of internal factors such as wettability, saturation and micro pore throat structure parameters on the rock electrical characteristics is analyzed. The experimental results show that the porosity-based image segmentation method is used to binarize the digital image of low permeability sandstone and establish the pore network model, which can retain the real topology of pore space to the greatest extent. Rock electrical three-dimensional simulation technology of porescale has certain applicability for complex reservoirs such as low permeability sandstone. The wettability, pore throat structure and other microscopic factors have an important influence on the rock electrical characteristics. The resistivity of water-wet reservoir is lower than that of gas-wet reservoir, and the difference is more obvious in the stage of low water saturation. Among the micro pore throat structure parameters, the throat radius and pore throat ratio show a good positive correlation with the resistivity index. With the increase of coordination number, the resistivity index decreases, which is particularly prominent under low water saturation. [ABSTRACT FROM AUTHOR]
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- 2022
49. Improved the removal of chromium by polymer epichlorohydrin-dimethylamine functionalized mesoporous silica
- Author
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Baoyu Gao Wengang Wang, Baoyu Gao, Qinyan Yue, Shanshan Zhang, Xing Xu, and Wengang Wang
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chemistry.chemical_classification ,chemistry.chemical_compound ,Chromium ,chemistry ,chemistry.chemical_element ,Epichlorohydrin ,Polymer ,Mesoporous silica ,Dimethylamine ,Nuclear chemistry - Published
- 2019
50. Combinative treatment of β-elemene and cetuximab is sensitive to KRAS mutant colorectal cancer cells by inducing ferroptosis and inhibiting epithelial-mesenchymal transformation
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Ting Pan, Zuyi Yang, Ting Jin, Xiaohui Chen, Xinbing Sui, Qin Zhang, Xuejie Li, Wengang Wang, Lili Yan, Yongqiang Li, Bi Chen, Jiao Feng, Peng Chen, Lvjia Zhuo, Shuiping Liu, Da Wang, Yitian Sun, Lingpan Kong, Mingming Zhang, Guohua Li, Tian Xie, Xingxing Huang, Ting Duan, Yu Xiang, Xiaying Chen, Ruonan Zhang, Wenzheng Zhang, and Liuxi Chen
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0301 basic medicine ,Programmed cell death ,Epithelial-Mesenchymal Transition ,Colorectal cancer ,Cell ,Medicine (miscellaneous) ,Cetuximab ,Mice, Nude ,colorectal cancer ,epithelial-mesenchymal transformation ,medicine.disease_cause ,Proto-Oncogene Proteins p21(ras) ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Growth factor receptor ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Animals ,Humans ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,neoplasms ,Cell Proliferation ,Mice, Inbred BALB C ,β-elemene ,KRAS mutation ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,ferroptosis ,ErbB Receptors ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,Mutation ,Cancer research ,Drug Therapy, Combination ,Female ,KRAS ,Carcinogenesis ,Elemene ,Colorectal Neoplasms ,Sesquiterpenes ,medicine.drug ,Research Paper - Abstract
Background and Purpose: RAS mutations limit the effectiveness of anti-epidermal growth factor receptor (EGFR) monoclonal antibodies in combination with chemotherapy for metastatic colorectal cancer (mCRC) patients. Therefore, new cell death forms have focused on identifying indirect targets to inhibit Ras-induced oncogenesis. Recently, emerging evidence has shown the potential of triggering ferroptosis for cancer therapy, particularly for eradicating aggressive malignancies that are resistant to traditional therapies. Methods: KRAS mutant CRC cell HCT116 and Lovo were treated with cetuximab and β-elemene, a bioactive compound isolated from Chinese herb Curcumae Rhizoma. Ferroptosis and epithelial-mesenchymal transformation (EMT) were detected in vitro and in vivo. Orthotopic CRC animal model were established and the tumor growth was monitored by IVIS bioluminescence imaging. Tumor tissues were collected to determine ferroptosis effect and the expression of EMT markers after the treatment. Results: CCK-8 assay showed that synergetic effect was obtained when 125 µg/ml β-elemene was combined with 25 µg/ml cetuximab in KRAS mutant CRC cells. AV/PI staining suggested a non-apoptotic mode of cell death after the treatment with β-elemene and cetuximab. In vitro, β-elemene in combination with cetuximab was shown to induce iron-dependent reactive oxygen species (ROS) accumulation, glutathione (GSH) depletion, lipid peroxidation, upregulation of HO-1 and transferrin, and downregulation of negative regulatory proteins for ferroptosis (GPX4, SLC7A11, FTH1, glutaminase, and SLC40A1) in KRAS mutant CRC cells. Meanwhile, combinative treatment of β-elemene and cetuximab inhibited cell migration and decreased the expression of mesenchymal markers (Vimentin, N-cadherin, Slug, Snail and MMP-9), but promoted the expression of epithelial marker E-cadherin. Moreover, ferroptosis inhibitors but not other cell death suppressors abrogated the effect of β-elemene in combination with cetuximab on KRAS mutant CRC cells. In vivo, co-treatment with β-elemene and cetuximab inhibited KRAS mutant tumor growth and lymph nodes metastases. Conclusions: Our data for the first time suggest that the natural product β-elemene is a new ferroptosis inducer and combinative treatment of β-elemene and cetuximab is sensitive to KRAS mutant CRC cells by inducing ferroptosis and inhibiting EMT, which will hopefully provide a prospective strategy for CRC patients with RAS mutations.
- Published
- 2020
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