1. Combined analysis of gut microbiota and metabolomics in high-fat model mice fed with Chlorella pyrenoidosa peptides
- Author
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Wenxiang Liu, Jiaying Wang, Qi Gao, Wenjuan Shen, Peifang Weng, Zufang Wu, Wenyan Qin, and Yanan Liu
- Subjects
Chlorella pyrenoidosa peptides ,Obesity ,Gut microbiota ,Metabolites ,High-fat-diet mice ,Blood lipids ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Chlorella pyrenoidosa peptides (SISISVAGGGR, T1) can effectively alleviate obesity and related metabolic disorders in high-fat mice. However, their lipid-lowering mechanism is still unclear. This research endeavor sought to delve into the potential mediators of T1 anti-obesity in high-fat mice through macrogenomics analyses combined with untargeted metabolomics analyses. The results showed that T1 alleviated HFD-induced increases in body weight, epididymal fat weight, TG, LDL, AST, TNF-α, and IL-1β levels, and increased HDL level. Moreover, T1 reversed the HFD-induced gut microbiota dysbiosis. Specifically, T1 resulted in a massive proliferation of Bacteroides, Parabacteroides, and Alistipes, as well as increased levels of metabolites such as DL-arginine, N-stearoyl GABA, and decreased levels of 7α,24(S)-dihydroxy-4-cholesten-3-one, and hexadecanedioic acid. Correlation analysis showed that T1 alleviated HFD-induced obesity metabolic syndrome and associated inflammation by modulating specific gut microbiota and related metabolites. This provides new ideas for the treatment of diet-induced obesity and related metabolic disorders.
- Published
- 2024
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