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Introduction: The modulation of post-training fatigue in colleges and universities is an important part of today's physical education. The adjustment of sports fatigue is a fundamental aspect of modern college physical education, and its control is of great importance for the elevation in the sport level of athletes. Objective: Explore the effects of nutritional intervention on post-training fatigue in college athletes. Methods: 40 athletes were randomly selected as volunteers for the research, divided into control and experimental group, and practiced the same type of exercise and same intensity. The athletes in the experimental group took food in strict accordance with the food mixture described in the article, while the control group kept their regular diet unchanged. After the experiment, sports training was performed, followed by muscle creatine enzyme measurement and laboratory analysis of blood urea. These data were compared and analyzed with those before the experiment. Results: After adjusting the dietary structure, the CK and Bu indices of the athletes in the experimental group showed a downward trend, indicating that adjusting the nutritional structure can effectively improve the post-training fatigue of college athletes. Conclusion: It is recommended that physical education teachers and college coaches adjust the lifestyle and dietary structure according to the actual situation of the students, aiming to promote integral development and improved sports performance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. RESUMO Introdução: A modulação da fadiga pós-treino nas faculdades e universidades é uma parte importante da atual educação física universitária. O ajuste da fadiga esportiva é um aspecto fundamental da educação física universitária moderna e seu controle é de grande importância para a elevação no nível esportivo dos atletas. Objetivo: Explorar os efeitos da intervenção nutricional sobre a fadiga pós-treino dos atletas universitários. Métodos: Foram selecionados 40 atletas aleatoriamente como voluntários para a pesquisa, divididos em grupo controle e experimental, praticaram o mesmo tipo de exercício físico e com a mesma intensidade. Os atletas do grupo experimental tomaram alimentos em estrita conformidade com a mistura alimentar descrita no artigo, enquanto o grupo de controle manteve sua alimentação regular inalterada. Após o experimento, foi realizado um treinamento esportivo, seguido de aferição da enzima creatina muscular e análise laboratorial de ureia no sangue. Esses dados foram comparados e analisados com os anteriores ao experimento. Resultados: Após o ajuste da estrutura dietética, os índices de CK e Bu dos atletas do grupo experimental mostraram uma tendência descendente, indicando que o ajuste da estrutura nutricional pode efetivamente melhorar a fadiga pós-treino dos atletas universitários. Conclusão: Recomenda-se aos professores de educação física e treinadores universitários ajustarem os hábitos de vida e estrutura alimentar de acordo com a situação real dos alunos, visando a promoção do desenvolvimento integral e melhora no desempenho esportivo. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Resumen Introducción: La modulación de la fatiga post-entrenamiento en colegios y universidades es una parte importante de la educación física universitaria actual. El ajuste de la fatiga deportiva es un aspecto fundamental de la educación física universitaria moderna y su control es de gran importancia para la elevación del nivel deportivo de los atletas. Objetivo: Explorar los efectos de la intervención nutricional sobre la fatiga post-entrenamiento en atletas universitarios. Métodos: 40 atletas fueron seleccionados al azar como voluntarios para la investigación, divididos en grupo control y experimental, practicaron el mismo tipo de ejercicio físico y con la misma intensidad. Los atletas del grupo experimental tomaron alimentos siguiendo estrictamente la mezcla de alimentos descrita en el artículo, mientras que el grupo de control mantuvo su dieta habitual sin cambios. Tras el experimento, se llevó a cabo una sesión de entrenamiento deportivo, seguida de la medición de la enzima creatina muscular y el análisis de laboratorio de la urea en sangre. Estos datos se compararon y analizaron con los anteriores al experimento. Resultados: Tras ajustar la estructura dietética, los índices de CK y Bu de los atletas del grupo experimental mostraron una tendencia descendente, lo que indica que el ajuste de la estructura nutricional puede mejorar eficazmente la fatiga post-entrenamiento de los atletas universitarios. Conclusión: Se recomienda que los profesores de educación física y los entrenadores universitarios ajusten el estilo de vida y la estructura nutricional de acuerdo con la situación real de los estudiantes, con el objetivo de promover el desarrollo integral y la mejora del rendimiento deportivo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

The existing studies lack research on the ductility of steel-reinforced high-strength concrete (SRHC) columns and current specifications restricted the use of high-strength concrete in steel-reinforced concrete (SRC) columns. To compensate for the shortcomings of the existing research and promote the application of high-strength concrete in SRC structures, we test six SRHC columns and one SRC column to examine the effects of the steel content, eccentric distance, and slenderness ratio on the ductility, bearing capacity, and failure mode of SRHC columns. Further, Abaqus finite element models are established to predict the influences of more parameters on post-peak ductility and analyze the relationship between strain development of the concrete and the decrease in bearing capacity of SRHC columns. The results show that the penetration of cracks into aggregate during failure is the primary reason for the poor ductility of the SRHC columns. Improving the confinement effect of the stirrups on concrete is the most effective measure to enhance the ductility of the SRHC columns. The decline in the stirrup spacing from 100 mm to 50 mm increased the ductility coefficient from 1.47 to 5.56. The effect of the steel content, stirrup strength, and slenderness ratio on the ductility coefficient of SRHC columns is less than 30%. After analyzing the reason for the error of current specifications, a modified formula with an error of less than 5% is developed.

Educational informatization has become the only way for mankind to enter the information age, and the application of educational resource library is a key issue in the integration of information technology disciplines. Aiming at the shortcomings of existing college physical education management technology, this article is dedicated to developing a new online management platform for physical education. Firstly, the introduction of edge computing technology in the architecture design of college physical education system can fully improve the efficiency of the management system. Secondly, based on data cleaning, the BP neural network algorithm modified by particle swarm is used to conduct in-depth analysis of the data of the university sports teaching management system and obtain corresponding optimization measures. Finally, it is verified in actual tests that the system can quickly perform data search, entry, modification, deletion, and other operations, which can ensure the realization of the school sports department’s networked, scientific, standardized, and digitalized management of physical education resources.

Increasing number of studies report that microRNAs play important roles in radiosensitization. miR-30a has been proved to perform many functions in the development and treatment of cancer, and it is downregulated in non-small cell lung cancer (NSCLC) tissues and cells. This study was conducted to understand if miR-30a plays a role in the radiosensitivity of NSCLC cells. Radiosensitivity was examed by colony survival assay and tumor volume changing in vitro and in vivo, respectively. Bioinformatic analysis and luciferase reporter assays were used to distinguish the candidate target of miR-30a. qRT-PCR and western blotting were carried out to detect the relative expression of mRNAs and proteins. Cell cycle and cell apoptosis were determined by flow cytometry. Our results illustrated miR-30a could increase the radiosensitivity of NSCLC, especially in A549 cell line. In vivo experiment also showed the potential radiosensitizing possibility of miR-30a. Further exploration validated that miR-30a was directly targ-eting activating transcription factor 1 (ATF1). In studying the ataxia-telangiectasia mutated (ATM) associated effects on cell radiosensitivity, we found that miR-30a could reduce radiation induced G2/M cell cycle arrest and may also affect radiation induced apoptosis. Together, our results demonstrated that miR-30a may modulate the radiosensitivity of NSCLC through reducing the function of ATF1 in phosphorylation of ATM and have potential therapeutic value.

Lung cancer is a common type of cancer with a high mortality rate in China. Cisplatin (Cis) is one of the most effective broad‑spectrum chemotherapeutic drugs for the treatment of advanced lung cancer. However, Cis resistance remains an obstacle in the treatment of advanced lung cancer. Pristimerin (Pris), a naturally occurring triterpenoid quinone compound, not only possesses anticancer properties, but also enhances chemosensitivity. Therefore, the present study aimed to investigate whether Pris can enhance the chemosensitivity of lung cancer cells to Cis and identify the underlying mechanism. A Cell Counting kit‑8 and flow cytometry were used to determine cell viability, cell cycle progression and apoptosis in A549 and NCI‑H446 cells. Western blotting was used to determine cell apoptosis‑related, cell cycle‑related and autophagy‑related proteins. The results showed that Pris inhibited cell proliferation, and induced G0/G1 arrest and cell apoptosis in A549 and NCI‑H446 cells. The western blotting revealed that Pris effectively synergized with Cis to induce cell apoptosis by inhibiting the microRNA‑23a/Akt/glycogen synthase kinase 3β signaling pathway and suppressing autophagy. In vivo xenograft experiments confirmed that Pris effectively synergized with Cis to suppress tumor growth. Collectively, these results indicate that Pris synergized with Cis and that this may be a potential therapeutic strategy to overcome lung cancer.

Background In cervical cancer patients with intermediate-risk factors, the optimal adjuvant therapy is still controversial. We undertook a randomized trial (ClinicalTrials.gov Identifier: NCT01418859) to compare the efficacy and toxicity of concurrent chemoradiotherapy with topotecan and cisplatin with radiotherapy alone in intermediate-risk cervical cancer patients. Methods Eligible patients were randomly assigned to one of three treatment arms including arm A (radiotherapy only,RT), arm B(concurrent chemoradiotherapy only, CCRT), and arm C (concurrent chemoradiotherapy with following consolidation chemotherapy, CCRT + CT). All eligible patients completed external RT (IMRT or 3D-CRT), receiving 45-50Gy /25f uniformly to the pelvis. Concurrent chemotherapy regimen was topotecan 0.75 mg/m2 for days 1, 2 and 3, followed by cisplatin 25 mg/m2 for days 1, 2 and 3. Three cycles of consolidation chemotherapy regimen was topotecan 1.5 mg/m2 for days 1 and 2, and 0.75 mg/m2 for day 3; followed by cisplatin 25 mg/m2 for days 1, 2 and 3, repeated every 21 days. Adverse events of each group were investigated and compared. Results Thirty-nine patients enrolled onto the remaining regimens: 14 to RT, 15 to CCRT and 10 to CCRT + CT. Six patients (15.4%) did not complete the protocol treatment. Hematologic toxicity was more frequent and more severe in the CCRT and CCRT + CT arms compared with the RT arm. The incidence of grade 3-4 neutropenia was significantly different statistically between the RT, CCRT and CCRT + RT groups (15.4%, 46.7% and 100%, respectively; P = 0.002). Specially, three patients in CCRT + CT arm of all six patients who did not complete the protocol treatment discontinued planned therapy because of persistent grade 4 neutropenia. However, there were no significant differences in grade 3-4 non-hematologic toxicities between the three groups(all P > 0.05). Recurrence-free survival and overall survival of each group were not analyzed on account of a median follow-up of only 16 months. Conclusions Concurrent chemoradiotherapy with topotecan and cisplatin showed severe hematologic toxicity in intermediate-risk cervical cancer patients after radical hysterectomy. Thus, the study was closed ahead of schedule. Trial registration ClinicalTrials.gov Identifier: NCT01418859.

Wenze Sun,1 Ting Ai,2 Ying Gao,1 Yingbing Zhang,1 Jie Cui,1 Liping Song1 1Department of Radiation Oncology, 2Department of Neonatology, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an, People's Republic of China Background: MET is involved in the progression of several types of human cancers, while phospho-BAD(Ser-136) is a key molecule in apoptosis and might be regulated by MET. The aim of this study was to investigate the correlation between altered expression of MET and phospho-BAD in non-small cell lung cancer (NSCLC) and their association with clinicopathologic parameters and overall survival. Methods: MET and phospho-BAD(Ser-136) proteins were evaluated by immunohistochemical analysis in 183 paraffin-embedded specimens and were also assessed by Western blotting analysis in 12 frozen tumor tissue samples, which were representative examples of immunohistochemical staining. Results: Positive expression of MET and phospho-BAD(Ser-136) occurred in 67.2% and 49.2% of the 183 cases of NSCLC, respectively. However, neither MET expression nor phospho-BAD(Ser-136) expression was associated with any clinicopathologic parameter. A significant correlation was found between MET and phospho-BAD(Ser-136) expression levels evaluated by immunohistochemistry (r = 0.268, P < 0.001). Overexpression of MET was significantly associated with shortened overall survival in univariate analysis (P < 0.001). Moreover, patients with a MET+/phospho-BAD(Ser-136)+ phenotype had a poorer prognosis than others (P < 0.001). Multivariate Cox proportional hazard analysis confirmed that MET expression is a prognostic factor for NSCLC. Conclusion: MET expression might be correlated with phospho-BAD(Ser-136) expression, and may be an adverse predictor for NSCLC. Activation of the MET/phospho-BAD(Ser-136) signaling pathway might play a role in the development and progression of NSCLC. Keywords: MET, phospho-BAD, prognosis, non-small cell lung cancer

To determine the influence of different registration scopes and methods on kilo-voltage cone beam CT (kVCBCT) image and CT image and to estimate the appropriate clinical target volume (CTV)-to-planned target volume (PTV) margin for central-type non-small cell lung cancer in image-guided radiation therapy (IGRT).Twenty-six patients with central-type non-small cell lung cancer (NSCLC) who received IGRT were enrolled in this study and totally 104 flames of kVCBCT scanning acquired before radiotherapy were analyzed. First, registrations were performed by gray plus manual match and were compared among different registration scopes of tumor and tumor plus vertebra. Then, the results of registration as well as time cost using tumor plus vertebra as registration scope were compared among different registration methods of gray plus manual match and bone plus manual match. At last, 200 registrations using tumor plus vertebra as the registration scope performed by bone plus manual match were recorded and the CTV-to-PTV margin was calculated.There was no significant difference in X, Y and Z translational and rotational movements between the registration scopes of tumor and tumor plus vertebra when gray plus manual match was used as the registration method (P0.05). The registration results of gray plus manual match and bone plus manual match were equal when tumor plus vertebra was used as the registration scope (P0.05), but the time cost of bone plus manual match [(1.9±0.3) min] was shorter than that of gray plus manual match [(3.1±0.2) min]. The CTV-to-PTV margins were 5.3, 4.9 and 5.7 mm in X, Y, and Z directions respectively.For central-type NSCLC, tumor and vertebra can be used as registration scope and the bone plus manual match is suggested in IGRT. To avoid errors, we suggest a CTV-to-PTV margin of 6 mm.

Lung cancer is the major cause of cancer deaths worldwide due to its late diagnosis and poor outcome. Understanding genomic medicine may widen our vision into the oncogenesis of lung cancer and may open the door to improvements in the clinical management of lung cancer. It is well known that almost half of all genes are regulated by microRNAs (miRNAs). This review focuses on the role of miRNAs in lung cancer and also touches on the value of miRNA-based novel therapies for lung cancers.