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Host-associated microbiota can biotransform xenobiotics, mediate health effects of chemical exposure, and play important roles in early development. Bisphenol A (BPA) is a widespread environmental chemical that has been associated with adverse endocrine and neurodevelopmental effects, some of which may be mediated by microbiota. Growing public concern over the safety of BPA has resulted in its replacement with structurally similar alternatives. In this study, we evaluated whether BPA and BPA alternatives alter microbiota and modulate secondary adverse behavioral effects in zebrafish. Zebrafish were developmentally exposed to BPA, Bisphenol AF (BPAF), Bisphenol B (BPB), Bisphenol F (BPF), or Bisphenol S (BPS). At 10 days post fertilization (dpf), toxicity assessments were completed and 16S rRNA gene sequencing was performed to evaluate potential chemical-dependent shifts in microbial community structure and predicted function. A standard light/dark behavioral assay was used to assess locomotor activity. Based on developmental toxicity assessments at 10 dpf, a range of potencies was observed: BPAF > BPB > BPF ∼ BPA > BPS. Analysis of 16S rRNA gene sequencing data showed significant concentration-dependent disruption of microbial community structure and enrichment of putative microbial functions with exposure to BPS, BPA, or BPF, but not BPB or BPAF. Interestingly, microbial disruption was inversely related to host developmental toxicity and estrogenicity. Exposure to BP analogs did not cause behavioral effects at 10 dpf. Our findings indicate that some BP analogs disrupt host microbiota early in life and demonstrate novel chemical-microbiota interactions that may add important context to current hazard identification strategies. [ABSTRACT FROM AUTHOR]

Onsite non-potable water reuse systems (ONWS) are decentralized systems that treat and repurpose locally collected waters ( e.g. greywater or combined wastewater) for uses such as irrigation and flushing toilets. To ensure that treatment is meeting risk benchmarks, it is necessary to monitor the efficacy of pathogen removal. However, accurate assessment of pathogen reduction is hampered by their sporadic and low occurrence rates in source waters and concentrations in treated water that are generally below measurement detection limits. An alternative metric for evaluation of onsite water treatment is log reduction of a more abundant organism that can serve as a surrogate for the pathogen removal. Viruses endogenous to the decentralized system could serve as monitoring surrogates to verify that treatment meets the relevant viral log reduction targets. This study assesses eight candidate PCR targets representing potential monitoring surrogates from different viral classes to determine whether they could be used to verify the efficacy of treatment in onsite non-potable water reuse systems. Candidates tested include markers for Carjivirus (formerly CrAssphage), Pepper Mild Mottle Virus (PMMoV), Microviridae, and T4 Coliphage. We quantified these targets in untreated influent wastewater at three onsite non-potable water reuse systems, two that use greywater and one that uses combined wastewater. We also confirmed, using amplicon sequencing, that the widely used Carjivirus and PMMoV primers correctly target their respective regions of interest, and found sequence diversity within the amplicons including in the probe binding region. Ultimately, we found that the surrogates assessed are not abundant enough for end uses with higher exposure use and concomitant greater removal requirements ( e.g. , indoor non-potable uses), but may be effective for end uses where exposure risk is lower ( e.g. , irrigation)., Competing Interests: Conflicts of interest There are no conflicts to declare.