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Background: Soybean cultivars grown on various crop rotation models in the agroforestry system with kayu putih (Melaleuca cajuputi) have shown different yields per hectare. However, no information related to morpho-physiological and biochemical fingerprints affecting soybean yield has been found. Thus, this study aimed to determine the morpho-physiological and biochemical fingerprints and their effect on the soybean agroforestry system in different crop rotation models through multivariate analysis. Methods: The study was conducted during the dry season (March-June 2021) and the wet season (November 2021-February 2022) in Menggoran Forest Resort, Playen Forest Section, Yogyakarta Forest Management District, Indonesia. The morpho-physiological and biochemical variables of 15 soybean cultivars were evaluated using four crop rotation models. Observations included 22 morphological, physiological and biochemical variables of soybean. Data were analyzed using ANOVA, PCA-biplot, heatmap cluster, factor analysis, SEM-PLS and standardized stepwise regression. Result: Results showed four groups of soybean cultivars and three groups of crop rotation models based on morpho-physiological and biochemical fingerprints. Morpho-physiological and biochemical fingerprints of soybean can be differentiated based on root surface area, nitrogen content and superoxide dismutase. [ABSTRACT FROM AUTHOR]

Shallot is one of the staple commodities in Indonesia. Increasing consumption needs and high demand for shallots represent a potential market opportunity for farmers to increase shallot production. The research aimed to determine farmers’ perceptions, the influence of farmers’ internal and external characteristics on perceptions of applying shallot cultivation technology, and trends in technology adoption. The research was conducted in Karang Kalasan Village, Kalasan District, Sleman Regency, D.I. Yogyakarta from March to May 2021. Data was collected through a survey using a questionnaire from 25 participants. Data were analyzed descriptively, percentages, frequency tables, Likert scales, and Spearman Rank correlation. The research showed that farmers had a positive perception of technological innovation in shallot cultivation. Farmers considered that shallot cultivation technology is profitable, suits people’s needs, is not complicated, is triable and the results are observable. The characteristics of gender, length of experience in shallot farming, farmer income, availability of infrastructure, and institutional support significantly influenced farmers’ perceptions on the application of technology innovation in shallot cultivation. Adoption trends indicate that some components will be adopted in the next growing season.

Untreated anemia in pregnant women potentially lead to complications of pregnancy and childbirth. Iron deficiency is one of the main causes of anemia. Honey has low pH, so it is expected to increase absorption of iron from Fe tablets consumed by pregnant women. The purpose of this study was to determine the effectiveness of acacia honey consumption in increasing hemoglobin levels of pregnant women with anemia. Acacia honey was produced from the nectar of Acacia crassicarpa. The study was a quasi-experiment with pre-post with control group design. The study was preceded by ethical testing and informed consent. The respondents were 30 pregnant women who did antenatal care and checked their pregnancy at Marinir Cilandak Hospital, South Jakarta. Respondents who had hemoglobin levels below 11 g/dL were included in this study. Respondents were further divided into two groups of 15 each. The treatment group consumed one Fe tablet and acasia honey 2×10 mL daily, while the control group consumed one Fe tablet daily. The study was conducted for 14 days. The independent t-test showed the hemoglobin levels increased significantly (p=0.031) in the group that consumed honey compared to the control group. Hemoglobin levels in pregnant women who consumed acacia honey and Fe tablets were higher than consumed Fe tablets only.

Indonesian workers better known as migrant workers from Indonesia who work in Singapore need to receive entrepreneurship education to encourage these migrant workers to improve one’s quality of life going forward, especially after returning to their homeland. Entrepreneurship training is a type of education that supports the growth of migrant workers’ entrepreneurial ambitions by offering opportunity to build businesses through opportunities and needs research of the market. This education hopes that migrant workers will earn income after working in Singapore. Apart from that, migrant workers also need to obtain good financial literacy. Financial literacy can support prosperity in the future and help achieve prosperity for the family. In Singapore, migrant workers’ financial management practices are more than adequate. Financial self-efficacy and financial literacy have an impact on migrant workers’ money management practices in Singapore. A hedonistic lifestyle does not show an impact on financial behavior. Therefore, in order to improve migrant workers’ welfare in the long run, the government must increase financial literacy and their capacity to make financial decisions.

Dry land in Gunungkidul is the land that depends on rainfall as the main water source to the crops. This research aimed to evaluate the implementation of supplementary water irrigation and rice husk for improving soil quality. The study conducted at dry land of Wonosari-Gunungkidul on June-Sept 2019. Experimental Design used Randomized Completely Block and replicated four times. The treatments were frequency of irrigation, namely F1 (1 times), F2 (2 times), F3 (3 times) a week, and application of soil amendment, namely: M1 (without mulch), M2 rice straw 4 ton.ha-1), M3 (rice husks 4 ton.ha-1), M4 (straw mulch + rice husk 4 ton.ha-1). The result of study showed that applying straw much and rice husk plus supplementary water irrigation two times a week was able to provide the highest corn production (7.46 ton.ha-1) and was not significantly different to the irrigation three times a week and showed an increased yield of corn 52.56% compared to control (watering once a week and without mulch/rice husk). Applying straw and rice husk can be improved the chemical soil properties (increasing water holding capacity,C-organic, P and K available and CEC).The financial farming of BC ratio reached 2.25 and increased 144.56% compare to control treatment.

We developed innovative self-amplifying mRNA (sa-mRNA) vaccine based on the derivative of S and Nsp3 proteins, which are considered crucial adhering to human host cells. We performed B-cell, Major histocompatibility complex (MHC) I, and II epitope which were merged with the KK and GPGPG linker. We also incorporated 5' cap sequence, Kozak sequence, replicase sequence, 3'/5' UTR, and poly A tail within the vaccine structure. The vaccine structure was subsequently docked and run the molecular dynamic simulation with TLR7 molecules. As the results of immune response simulation, the immune response was accelerated drastically up to >10-fold for immunoglobulin, interferon-γ, interleukin-2, immunoglobulin M (IgM) + immunoglobulin G (IgG) isotype, IgM isotype, and IgG1 isotype in secondary and tertiary dose, whereas natural killer cells, macrophages, and dendritic cells showed relatively high concentrations after the first dose. As our finding, the IgM + IgG, IgG1 + IgG2, and IgM level (induced by sa-mRNA vaccine) ensued three times with two-fold increase in days 25, and 50, then decreased after days 70-150. However, 150-350 days demonstrated constantly in the range of 20,000-21,000., Competing Interests: There are no conflicts of interest., (Copyright: © 2024 Journal of Advanced Pharmaceutical Technology & Research.)

In the event of a radiological incident, a fast and accurate biological dosimetry (biodosimetry) method for evaluating people who have been potentially exposed to ionising radiation is crucial. Among the many biodosimetry methods available, the immunodetection of phosphorylated H2AX (γ-H2AX) stands as a promising method to be used in the triage of patients exposed to radiation. Currently, the most common way to measure γ-H2AX levels is through fluorescence microscopy. In this pilot study, we assessed the feasibility of using an enzyme-linked immunosorbent assay (ELISA) for quantifying γ-H2AX for biodosimetry purposes. Moreover, the usefulness of measuring phosphorylated ATM (pATM) levels through ELISA for biodosimetry was also evaluated. Blood samples were obtained from three male donors (38 y) and were irradiated with 60Co (0, 1, 2 and 6 Gy). Peripheral blood mononuclear cells (PBMCs) were isolated and lysed before measuring γ-H2AX, total H2AX protein and pATM using ELISA kits. The dicentric chromosome assay (DCA) using whole blood was also performed for comparison. Data from all donors at each dose were pooled before statistical analysis. The ratio of γ-H2AX/total H2AX and pATM levels increased in a radiation-dose-dependent manner. The average γ-H2AX/total H2AX ratios were 0.816 ± 0.219, 0.830 ± 0.685, 1.276 ± 1.151 and 1.606 ± 1.098, whereas the average levels of pATM were 59.359 ± 3.740, 63.366 ± 0.840, 66.273 ± 2.603 and 69.936 ± 4.439, in PBMCs exposed to 0, 1, 2 and 6 Gy, respectively. The linear-quadratic dose-response calibration curve for DCA was Y = 0.0017 (±0.0010) + 0.0251 (±0.0142) × D + 0.0342 (±0.0039) × D2  $\boldsymbol{Y}=\mathbf{0.0017}\left(\pm \mathbf{0.0010}\right)+\mathbf{0.0208}\left(\pm \mathbf{0.0218}\right)\times \boldsymbol{D}+\mathbf{0.0350}\left(\pm \mathbf{0.0050}\right)\times{\boldsymbol{D}}^{\mathbf{2}}$. Overall, despite a large variability in the ratio of γ-H2AX/total H2AX among donors, the present study revealed the suitability of using the ratio of γ-H2AX/total H2AX and pATM for biodosimetry. Still, more research with a larger group of subjects is necessary to construct a reliable calibration curve for the ratio of γ-H2AX/total H2AX and pATM levels for biodosimetry., (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

On Earth, there are significant variations in terms of exposure to naturally occurring radiation among different areas. Radon, a naturally-occurring radioactive gas that is the primary cause of lung cancer in nonsmokers and the second most prevalent cause among smokers, poses a considerable risk. Indoor radon, in particular, constitutes the most substantial source of natural radiation to which individuals are exposed. This study assessed the immune status of a population chronically exposed to high indoor radon concentration in Indonesia. Fifty-seven subjects from the Tande-Tande sub-village (high indoor radon concentration area) were compared to fifty-three participants living in the Topoyo village (low concentration area). We contrasted the immunological conditions of these two populations by measuring levels of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-4 (IL-4), and IL-10 in serum. Moreover, we also measured levels of the nuclear factor kappa B (NF-κB), superoxide dismutase (SOD), glutathione peroxidase (GPX), and protein kinase B in its phosphorylated (pAkt) and non-phosphorylated form (Akt) in peripheral blood mononuclear cells (PBMCs) of a subset of participants (31 from each population). TNF-α, IFN-γ, and IL-4 levels in Tande-Tande sub-village inhabitants were significantly lower than those in the control group living in the Topoyo village (p = 0.001, p = 0.017, and p = 0.002). The concentration of IL-10 also tended to be lower in people living in the high indoor radon concentration area, but it did not differ significantly between Tande-Tande sub-village inhabitants and Topoyo inhabitants (p = 0.106). Protein levels of NF-κB, pAkt, and Akt in Tande-Tande sub-village inhabitants also did not differ significantly between Tande-Tande sub-village inhabitants and Topoyo inhabitants (p = 0.234, p = 0.210, and p = 0.657). Similarly, activities of SOD and GPX did not differ significantly between the two populations (p = 0.569 and p = 0.949). Overall, despite their chronic exposure to high indoor radon concentrations, our study revealed no increase in the levels of TNF-α, IFN-γ, IL-10, IL-4, SOD, and GPX in the inhabitants of Tande-Tande sub-village compared with people living in the Topoyo village. Furthermore, our study demonstrated no activation in the Akt pathway, as indicated by the pAkt/Akt ratio observed in PBMC lysates of individuals residing in the Tande-Tande sub-village., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Objective: To identify factors related to the stress levels among pregnant women in Indonesia., Method: The study was used cross sectional design. The participants were 92 pregnant women who worked at a footwear manufacturer at Banten, Indonesia. Half of the participants worked less than 40h per week and the other half worked 40h or more per week. A test instrument to measure stress in pregnant women was developed and conducted in this study. Dependent and independent factors were analyzed by the chi-square test., Results: Our results showed that 59.78% of respondents had their gestational age was more than 31 weeks; 53.00% of workers experienced moderate stress; and as many 53.26% of respondents experienced a high workload., Conclusions: Our conclusion confirmed gestational age, workload, and working time related with work stress level of pregnant women significantly., (Copyright © 2020 SESPAS. Publicado por Elsevier España, S.L.U. All rights reserved.)

Objective:  The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons., Materials and Methods:  The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected C. albicans biofilm-associated genes ( ALS3 , HWP1 , and YWP1 )., Statistical Analysis:  The obtained data were analyzed by one-way analysis of variance, followed by Bartlett's test. When appropriate, the Student's t -test was also used; a value of p < 0.05 was considered statistically significant., Results:  In both groups, the count of C. albicans was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes ( ALS3 and HWP1 ) in the tongue dorsa was higher in the DW group ( p < 0.05), whereas the transcription level of the yeast-specific gene ( YWP1) was significantly higher in the NDW group., Conclusion:  Both tongue dorsa and dentures appear to be sharing factors that are important for C. albicans biofilm growth in abiotic and biotic oral surfaces of the elderly., Competing Interests: None declared.

Background: Atopic dermatitis (AD) is a chronic inflammation of the skin that often appears in early childhood. The manifestation is related to the tendency towards T helper 2 cytokine immune responses (interleukin (IL)-4, IL-5). Genetic factors are suggested to play important roles in AD, and it can be transmitted to newborns, increasing their risk of developing allergies., Objective: To determine the association between cord-blood cytokine levels (IL-5, interferon (IFN) γ), cord-blood total immunoglobulin E (IgE) level, perinatal environmental exposure, and the risks of allergy as well as the development of AD in the first 6 months of life., Methods: A 6-month cohort study with a nested case-control within was conducted on newborns in Jakarta from December 2008 until May 2009. After the umbilical cord blood samples were taken and stored, subjects were followed up monthly until 6 months old. The occurrence of AD and lifestyle or environmental exposures were recorded. The allergic risk was determined using a modified pediatric allergy immunology work groups scoring system based on allergic history (allergic rhinitis, asthma, AD) in the family. The levels of IL-5 and IFN-γ were measured using ELISA and total IgE by CAP system FEIA. Multivariate analysis was used to evaluate risk factors., Results: This study was conducted on 226 subjects. The incidence of AD was 16.4%; of those, 59% had low risk allergy, 38.5% moderate, and 2% high risk. AD mostly occurred at the age of 1 month (57%). Cord blood samples were examined in 37 subjects with AD and 51 without AD; of those, 25% showed high levels of total IgE (>1.2 IU/µL), and 51% showed normally-distributed high absorbance IL-5 values (≥0.0715, absolute value was undetected). The increased level of IL-5 was directly proportional to IgE. High absorbance IFN-γ values (≥0.0795, absolute value = 18.681 pg/µL) were observed in 52% of subjects., Conclusion: The associations between the risk of allergy in the family, cord-blood total IgE, IL-5, IFN levels, and some perinatal environmental exposure with AD in the first 6 months of life have not been established.