7 results on '"Willems, Monique G. M."'
Search Results
2. Systemic interleukin-2 administration improves lung function and modulates chorioamnionitis-induced pulmonary inflammation in the ovine fetus
- Author
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Willems, Monique G. M., primary, Ophelders, Daan R. M. G., additional, Nikiforou, Maria, additional, Jellema, Reint K., additional, Butz, Anke, additional, Delhaas, Tammo, additional, Kramer, Boris W., additional, and Wolfs, Tim G. A. M., additional
- Published
- 2016
- Full Text
- View/download PDF
3. Chorioamnionitis-induced fetal gut injury is mediated by direct gut exposure of inflammatory mediators or by lung inflammation
- Author
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Wolfs, Tim G. A. M., primary, Kramer, Boris W., additional, Thuijls, Geertje, additional, Kemp, Matthew W., additional, Saito, Masatoshi, additional, Willems, Monique G. M., additional, Senthamarai-Kannan, Paranthaman, additional, Newnham, John P., additional, Jobe, Alan H., additional, and Kallapur, Suhas G., additional
- Published
- 2014
- Full Text
- View/download PDF
4. Systemic interleukin-2 administration improves lung function and modulates chorioamnionitis-induced pulmonary inflammation in the ovine fetus.
- Author
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Willems MG, Ophelders DR, Nikiforou M, Jellema RK, Butz A, Delhaas T, Kramer BW, and Wolfs TG
- Subjects
- Animals, Female, Gestational Age, Lipopolysaccharides pharmacology, Pneumonia complications, Pneumonia immunology, Pregnancy, Sheep, Chorioamnionitis drug therapy, Fetus drug effects, Interleukin-2 pharmacology, Pneumonia drug therapy
- Abstract
Chorioamnionitis, an inflammatory reaction of the fetal membranes to microbes, is an important cause of preterm birth and associated with inflammation-driven lung injury. However, inflammation in utero overcomes immaturity of the premature lung by inducing surfactant lipids and lung gas volume. Previously, we found that lipopolysaccharide (LPS)-induced chorioamnionitis resulted in pulmonary inflammation with increased effector T cells and decreased regulatory T cell (Treg) numbers. Because Tregs are crucial for immune regulation, we assessed the effects of interleukin (IL)-2-driven selective Treg expansion on the fetal lung in an ovine chorioamnionitis model. Instrumented fetuses received systemic prophylactic IL-2 treatment [118 days gestational age (dGA)] with or without subsequent exposure to intra-amniotic LPS (122 dGA). Following delivery at 129 dGA (term 147 dGA), pulmonary and systemic inflammation, morphological changes, lung gas volume, and phospholipid concentration were assessed. IL-2 pretreatment increased the FoxP3(+)/CD3(+) ratio, which was associated with reduced CD3-positive cells in the fetal lungs of LPS-exposed animals. Prophylactic IL-2 treatment did not prevent pulmonary accumulation of myeloperoxidase- and PU.1-positive cells or elevation of bronchoalveolar lavage fluid IL-8 and systemic IL-6 concentrations in LPS-exposed animals. Unexpectedly, IL-2 treatment improved fetal lung function of control lambs as indicated by increased disaturated phospholipids and improved lung gas volume. In conclusion, systemic IL-2 treatment in utero preferentially expanded Tregs and improved lung gas volume and disaturated phospholipids. These beneficial effects on lung function were maintained despite the moderate immunomodulatory effects of prophylactic IL-2 in the course of chorioamnionitis., (Copyright © 2016 the American Physiological Society.)
- Published
- 2016
- Full Text
- View/download PDF
5. Responses of the spleen to intraamniotic lipopolysaccharide exposure in fetal sheep.
- Author
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Kuypers E, Willems MG, Jellema RK, Kemp MW, Newnham JP, Delhaas T, Kallapur SG, Jobe AH, Wolfs TG, and Kramer BW
- Subjects
- Amniotic Fluid drug effects, Animals, Apoptosis, CD3 Complex metabolism, Caspase 3 metabolism, Chorioamnionitis physiopathology, Cytokines metabolism, Female, Fetus metabolism, Gestational Age, Immune System, Inflammation, Interleukin-23 metabolism, Models, Animal, Pregnancy, Pregnancy, Animal, RNA, Messenger metabolism, Sheep, Spleen metabolism, Amniotic Fluid metabolism, Lipopolysaccharides chemistry, Spleen immunology
- Abstract
Background: Intrauterine inflammation activates the fetal immune system and can result in organ injury and postnatal complications in preterm infants. As the spleen is an important site for peripheral immune activation, we asked how the fetal spleen would respond to intrauterine inflammation over time. We hypothesized that intraamniotic lipopolysaccharide (IA LPS) exposure induces acute and persistent changes in the splenic cytokine profile and T-cell composition that may contribute to the sustained fetal inflammatory response after chorioamnionitis., Methods: Fetal sheep were exposed to IA LPS 5, 12, and 24 h and 2, 4, 8, or 15 d before delivery at 125 d of gestational age (term = 150 d). Splenic cytokine mRNA levels and cleaved caspase-3, CD3, and Foxp3 expression were evaluated., Results: IA LPS increased interleukin (IL)1, IL4, IL5, and IL10 mRNA by twofold 24 h after injection. Interferon gamma increased by fivefold, whereas IL23 decreased 15 d post-LPS exposure. Cleaved caspase-3-positive cells increased 2 and 8 d after LPS exposure. CD3 immunoreactivity increased within 5 h with increased Foxp3-positive cells at 12 h., Conclusion: Intrauterine inflammation induced a rapid and sustained splenic immune response with persistent changes in the cytokine profile. This altered immune status may drive sustained inflammation and injury in other fetal organs.
- Published
- 2015
- Full Text
- View/download PDF
6. Chorioamnionitis-induced fetal gut injury is mediated by direct gut exposure of inflammatory mediators or by lung inflammation.
- Author
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Wolfs TG, Kramer BW, Thuijls G, Kemp MW, Saito M, Willems MG, Senthamarai-Kannan P, Newnham JP, Jobe AH, and Kallapur SG
- Subjects
- Amniotic Fluid, Animals, Cell Differentiation, Cell Proliferation, Chorioamnionitis chemically induced, Cytokines genetics, Cytokines metabolism, Disease Models, Animal, Epithelial Cells cytology, Epithelial Cells drug effects, Female, Fetal Diseases chemically induced, Gastrointestinal Diseases embryology, Gastrointestinal Diseases pathology, Gene Expression Regulation, Developmental drug effects, Ileitis chemically induced, Ileitis embryology, Ileitis pathology, Ileum embryology, Ileum pathology, Inflammation chemically induced, Inflammation complications, Inflammation pathology, Intestinal Mucosa cytology, Lipopolysaccharides toxicity, Pneumonia chemically induced, Pneumonia pathology, Pregnancy, Random Allocation, Sheep, T-Lymphocytes, Regulatory, Toll-Like Receptors, Chorioamnionitis pathology, Fetal Diseases etiology, Gastrointestinal Diseases etiology, Pneumonia complications
- Abstract
Intra-amniotic exposure to proinflammatory agonists causes chorioamnionitis and fetal gut inflammation. Fetal gut inflammation is associated with mucosal injury and impaired gut development. We tested whether this detrimental inflammatory response of the fetal gut results from a direct local (gut derived) or an indirect inflammatory response mediated by the chorioamnion/skin or lung, since these organs are also in direct contact with the amniotic fluid. The gastrointestinal tract was isolated from the respiratory tract and the amnion/skin epithelia by fetal surgery in time-mated ewes. Lipopolysaccharide (LPS) or saline (controls) was selectively infused in the gastrointestinal tract, trachea, or amniotic compartment at 2 or 6 days before preterm delivery at 124 days gestation (term 150 days). Gastrointestinal and intratracheal LPS exposure caused distinct inflammatory responses in the fetal gut. Inflammatory responses could be distinguished by the influx of leukocytes (MPO(+), CD3(+), and FoxP3(+) cells), tumor necrosis factor-α, and interferon-γ expression and differential upregulation of mRNA levels for Toll-like receptor 1, 2, 4, and 6. Fetal gut inflammation after direct intestinal LPS exposure resulted in severe loss of the tight junctional protein zonula occludens protein 1 (ZO-1) and increased mitosis of intestinal epithelial cells. Inflammation of the fetal gut after selective LPS instillation in the lungs caused only mild disruption of ZO-1, loss in epithelial cell integrity, and impaired epithelial differentiation. LPS exposure of the amnion/skin epithelia did not result in gut inflammation or morphological, structural, and functional changes. Our results indicate that the detrimental consequences of chorioamnionitis on fetal gut development are the combined result of local gut and lung-mediated inflammatory responses.
- Published
- 2014
- Full Text
- View/download PDF
7. Altered canonical Wingless-Int signaling in the ovine fetal lung after exposure to intra-amniotic lipopolysaccharide and antenatal betamethasone.
- Author
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Kuypers E, Willems MG, Collins JJ, Wolfs TG, Nitsos I, Jane Pillow J, Polglase GR, Kemp MW, Newnham JP, Delhaas T, Jobe AH, Kallapur SG, and Kramer BW
- Subjects
- Animals, Betamethasone chemistry, Female, Glycogen Synthase Kinase 3 metabolism, Glycogen Synthase Kinase 3 beta, Inflammation, Lipopolysaccharides chemistry, Lung metabolism, Lymphoid Enhancer-Binding Factor 1 metabolism, Maternal Exposure, Phosphorylation, Pregnancy, Pregnancy, Animal, Sheep, Sheep, Domestic, Time Factors, Wnt Proteins metabolism, beta Catenin metabolism, Betamethasone administration & dosage, Lipopolysaccharides administration & dosage, Lung pathology, Wnt Signaling Pathway
- Abstract
Background: Antenatal inflammation and maternal corticosteroids induce fetal lung maturation but interfere with late lung development. Canonical Wingless-Int (Wnt) signaling directs lung development and repair. We showed that intra-amniotic (IA) lipopolysaccharide (LPS) exposure disrupted developmental signaling pathways in the preterm lamb lungs. Therefore, we hypothesized that pulmonary Wnt signaling was altered by exposure to IA LPS and/or antenatal corticosteroids., Methods: Ovine fetuses were exposed to IA LPS, maternal intramuscular betamethasone, a control saline injection, or a combination thereof at 107 and/or 114 d gestational age (term = 150 d gestational age) before delivery at 121 d gestational age., Results: IA LPS exposure decreased the lung expression of lymphoid enhancer-binding factor 1 (LEF1), a major Wnt pathway effector. WNT1, WNT4, and downstream messenger β-catenin decreased after LPS exposure. WNT7b mRNA increased fourfold 14 d post-LPS exposure. Betamethasone treatment 7 d before LPS exposure prevented the reduction in LEF1 expression, whereas betamethasone administration after LPS normalized the LPS-induced increase in Wnt7b mRNA., Conclusion: IA LPS exposure decreased canonical Wnt signaling in the developing lung. Antenatal corticosteroids before or after IA inflammation had different effects on pulmonary Wnt signaling. This study provides new insights into possible mechanisms by which prenatal inflammation affects lung development and how corticosteroid can be beneficial in this setting.
- Published
- 2014
- Full Text
- View/download PDF
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