1. From soil to sequence: filling the critical gap in genome-resolved metagenomics is essential to the future of soil microbial ecology
- Author
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Winston E. Anthony, Steven D. Allison, Caitlin M. Broderick, Luciana Chavez Rodriguez, Alicia Clum, Hugh Cross, Emiley Eloe-Fadrosh, Sarah Evans, Dawson Fairbanks, Rachel Gallery, Júlia Brandão Gontijo, Jennifer Jones, Jason McDermott, Jennifer Pett-Ridge, Sydne Record, Jorge Luiz Mazza Rodrigues, William Rodriguez-Reillo, Katherine L. Shek, Tina Takacs-Vesbach, and Jeffrey L. Blanchard
- Subjects
Soil Microbiome ,Hybrid Assembly ,Genome Resolved Metagenomics ,Microbiome Assembled Genomes ,FAIR Data Principles ,Environmental sciences ,GE1-350 ,Microbiology ,QR1-502 - Abstract
Abstract Soil microbiomes are heterogeneous, complex microbial communities. Metagenomic analysis is generating vast amounts of data, creating immense challenges in sequence assembly and analysis. Although advances in technology have resulted in the ability to easily collect large amounts of sequence data, soil samples containing thousands of unique taxa are often poorly characterized. These challenges reduce the usefulness of genome-resolved metagenomic (GRM) analysis seen in other fields of microbiology, such as the creation of high quality metagenomic assembled genomes and the adoption of genome scale modeling approaches. The absence of these resources restricts the scale of future research, limiting hypothesis generation and the predictive modeling of microbial communities. Creating publicly available databases of soil MAGs, similar to databases produced for other microbiomes, has the potential to transform scientific insights about soil microbiomes without requiring the computational resources and domain expertise for assembly and binning.
- Published
- 2024
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