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13. Physalis angulata leaf extract ameliorates L-NG-nitroarginine methyl ester (L-NAME)-induced preeclampsia symptoms in rats through improved endothelial progenitor cells and endothelial cells due to reduced antiangiogenic factor and oxidative stress

Author

Nugrahenny, Dian, primary, Rudijanto, Achmad, additional, Permatasari, Nur, additional, Wiyasa, I Wayan Arsana, additional, Widodo, Mohammad Aris, additional, Mintaroem, Karyono, additional, Widjajanto, Edi, additional, and Mustofa, Mustofa, additional

Published
2022
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18. Ethanolic extract of Moringa oleifera leaves improve inflammation, angiogenesis, and blood pressure in rat model of preeclampsia

Author

Wiyasa I. Wayan Arsana, Ahsan Ahsan, Santoso Sanarto, and Batmomolin Agnes

Subjects
Angiogenesis, business.industry, Rat model, Medicine (miscellaneous), Inflammation, Pharmacology, medicine.disease, Preeclampsia, Moringa, Blood pressure, medicine, Pharmacology (medical), General Pharmacology, Toxicology and Pharmaceutics, medicine.symptom, business
Published
2020

22. Genistein Affects Estrogen Receptor Alpha (ER-α)/Estrogen Receptor Beta (ER-β) Ratio, and Nuclear Factor-Kappa Beta (NF-κβ) in Mice Model of Endometriosis.

Author

Sutrisno, Sutrisno, Gayatri, Mergy, Wiyasa, I. Wayan Arsana, Kalsum, Umi, and Andarini, Sri

Subjects
ANIMAL disease models, LABORATORY mice, ESTROGEN receptors, GENISTEIN, ENDOMETRIOSIS
Abstract

Background: Currently, treatment of endometriosis remains expensive. One of low-cost treatments for this disease is genistein. This study aimed to assess the effect of genistein on Estrogen Receptor Alpha (ER-α) and Estrogen Receptor Beta (ER-β) ratio and Nuclear Factor-Kappa Beta (NF-κβ) in mice model of endometriosis remain unclear. Methods: This study is experimental using post-test research design. Twenty-four female mice (Mus musculus) model of endometriosis were fed genistein. The mice were divided into four groups with various doses of genistein, i.e., 1,30 mg/mice/day (group P1); 1,95 mg/mice/day (group P2); 2,60 mg/mice/day (P3), and; 3,25 mg/mouse/day (group P4). We fed the mouse using a sonde for 14 days. On the fifth day, the mice were sacrificed and dissected to take the peritoneal tissue. The tissue was coloured using Immunohistochemistry staining (IH) and microscopically assessed at 400x magnification calculated at 1000 cells in order to observe the expression of RE-α, RE-β, and NFkB. Results: Genistein at various doses had a significant effect on the RE-α / RE-β ratio. The higher the genistein dose given, the lower the RE-α / RE-β ratio. In addition, genistein dose of 3.25 mg / day significantly reduced NFkB expression. Conclusions: Genistein in various doses have been shown to significantly influence the RE-α / RE-β ratio. In other words, the higher the dose of genistein given will decrease the RE-α / RE-β ratio. [ABSTRACT FROM AUTHOR]

Published
2021

23. The correlation between estradiol, 25 hidroxyvitamin D and quality of life among postmenopausal women.

Author

Hartanti, Retno, Satiyem, Satiyem, Wiyasa, I. Wayan Arsana, Nurdiana, Nurdiana, Kalsum, Umi, Arifiandi, Maya Devi, Suryana, Bagus Putu Putra, Taufiq, Ahmad, Susanto, Hendra, Nur, Hadi, Aziz, Muhammad, Suksuwan, Acharee, Ng, Chen Siang, Jemon, Khairunadwa Binti, Amin, Mohamad, Diantoro, Markus, Mufti, Nandang, Malek, Nik Ahmad Nizam Nik, Wang, I Ching, and Sunaryono

Subjects
POSTMENOPAUSE, QUALITY of life, ESTRADIOL, ERGOCALCIFEROL, VITAMIN D, SAMPLING (Process)
Abstract

Estradiol decreases in postmenopausal women cause several changes in the physical, physiological, and psychological aspects. Consequently, various complaints emerged and affected women's quality of life (QoL). Vitamin D [25(OH)D] is an alternative to relieve menopausal complaints. This study aimed to examine the relationship between estradiol, 25(OH)D, and the QoL among postmenopausal women. This study applied a descriptive-analytic and consecutive sampling technique. The inclusion criteria were postmenopausal and physiological menopause women who were willing to participate in this study. The exclusion criteria were postmenopausal women with severe illness and under steroid treatments. ELISA, ECLIA, and MENQOL were used to examine 25(OH)D, estradiol, and QoL, respectively. The data were analyzed using SPSS version 23.0. The results showed thirty-nine postmenopausal women participated in this study. The average estradiol level was 5.68 pg/mL (SD ± 2.20), ranged from 5 to 16.07 pg/mL. There was a significant correlation between estradiol levels and QoL (r = −0.349; p = 0.029), and 25(OH)D and QoL (r = −0.383; p = 0.016). In conclusion, estradiol and 25(OH)D have a notable role in postmenopausal complaints. The QoL is a maJor consideration in postmenopausal women's life. [ABSTRACT FROM AUTHOR]

Published
2020
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25. Physalis Angulata Leaf Extract Protects Against Oxidative Stress and Antiangiogenic Factor in LNAME-Induced Preeclampsia Rats.

Author

NUGRAHENNY, DIAN, SETYAWATI SOEHARTO, PERMATASARI, NUR, RUDIJANTO, ACHMAD, WIYASA, I WAYAN ARSANA, WIDJAJANTO, EDI, WIDODO, MOHAMMAD ARIS, MINTAROEM, KARYONO, and MAYANGSARI, ELLY

Subjects
SYSTOLIC blood pressure, OXIDATIVE stress, PREECLAMPSIA, PHYSALIS, BLOOD pressure measurement, LABORATORY rats, LEAF physiology
Abstract

Objectives This study evaluated the protective effect of Physalis angulata leaf extract on L-NG-nitroarginine methyl ester (L-NAME)-induced preeclampsia rats. Methods Twenty-five pregnant Wistar rats were randomly divided into five groups. Preeclampsia rats were injected with L-NAME on the 5th to 17th day of gestation (G5-G17), while sham rats were injected with the same vehicle volume. Preeclampsia rats were administered with Physalis angulata leaf methanol extract at 500, 1500, or 2500 mg/kg body weight/day on G1-G17. The systolic blood pressure measurement was performed by the tail-cuff method. Placental VEGFR2 expression was determined by immunohistochemistry. The serum sFlt-1 level was evaluated using ELISA. Serum MDA level, SOD activity, and total nitrite level were measured by colorimetry. Results The L-NAME injection increased systolic blood pressure (1.44-fold), serum sFlt-1 level (1.22-fold), and serum MDA level (2.03-fold). It also decreased placental VEGFR2 expression (1.18-fold), serum SOD activity (2.11-fold), and serum nitrite level (1.25-fold) compared to the Sham group. The extract supplementation prevented the rise in systolic blood pressure and serum sFlt-1 level. It also tended to inhibit the reduction in the placental VEGFR2 expression. It prevented the increase in serum MDA level. It also prevented the decrease in serum SOD activity and nitrite level. Conclusions We concluded that Physalis angulata leaf methanol extract inhibits oxidative stress and an antiangiogenic factor in the rat preeclampsia model. These findings support that the extract has the potential to prevent preeclampsia development. [ABSTRACT FROM AUTHOR]

Published
2021
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26. The Effect of Alpha-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes

Author

Ratnawati, Rahajeng Siti Nur, Saputri, Ika Nur, Ratnawati, Retti, Soeharto, Setyawati, and Wiyasa, I Wayan Arsana

Subjects
lcsh:R5-920, oxidative stress, ovaries, endometrium, estrogen, antioxidant, lcsh:R, lcsh:Medicine, lcsh:Medicine (General), Oksidatif stres,overler,endometriyum,östrojen,antioksidan
Abstract

Purpose: The purpose of the current study was to evaluate the role of - tocopherol in inhibiting ovarian oxidative stress, changes in ovarian follicles, estradiol levels, and the thickness of the endometrium in rats exposed to tobacco smoke. Material and Methods: Forty female rats were divided into five groups consisting of a control group; group exposed to tobacco smoke; groups exposed to tobacco smokes receiving  - tocopherol supplementation at doses of 100; 200, and 400 mg/kg. Exposure to tobacco smoke was induced using smoke pumping equipment which was designed and made available in the Pharmacology Laboratory, Faculty of Medicine, Brawijaya University. Analysis of MDA level was done colorimetrically. Analysis of the number of ovarian follicles and endometrial thickness was done histopathologically with hematoxylin eosin staining. Analysis of estradiol level was done by ELISA technique. Results: Exposure to tobacco smoke in rats can increase ovarian MDA level (0.388 ± 0.085) significantly compared to the control (0.121 ± 0.026) (P> 0.05). -tocopherol in various doses can reduce MDA level significantly compared to the group exposed to tobacco smoke (P 0.05). The number of primary follicles in the -tocopherol group of the first dose (3.8 ± 1.095) was comparable to the control group (3.8 ± 1.095) (P> 0.05). The numbers of secondary follicles in the -tocopherol group of the first dose (5.2 ± 1.304) and of the second dose (5.4 ± 0.548) were significantly higher than the control group (3.6 ± 0.548) (P < 0.05). Tobacco smoke lowered estradiol level (10.815 ± 2.374) significantly compared with no exposure (21.354 ± 4.215) (P < 0.05). Administration of -tocopherol in different doses increased estradiol level, reaching the level in the control group (P > 0.05). Tobacco smoke lowered thickness of endometrium (209.491 ± 38.635) significantly compared with no exposure (600.265 ± 76.563) (P < 0.05). The -tocopherol of the first dose (604. 569 ± 33.621) and of the second dose (609.459 ± 37.105) increase estradiol levels, reaching the level in the control group (600.265 ± 76.563) (P > 0.05). Conclusion: Regarding the results describe above, it was concluded that -tocopherol could prevent oxidative damage and loss of ovarian function due to exposure to tobacco smoke characterized by decreased levels of MDA, increases in primary and secondary follicles, as well as the hormone estradiol. Furthermore, it also increased the thickness of endometrium., Amaç: Bu çalışmanın amacı, sigara dumanına maruz kalan sıçanlarda -tokoferolün overlerin oksidatif stresi, over foliküllerinin değişiklikleri ile östradiol düzeyleri ve endometriyum kalınlığı üzerine etkilerini değerlendirmektir. Materyal ve Metod: Kırk dişi sıçan beş gruba ayrıldı. Bu beş grup içerisine kontrol grubu ve sigara dumanına maruz bırakılan gruplar; 100, 200 ve 400 mg/kg dozlarında -tokoferol takviyesi yapılan, dahil edildi. Sigara dumanı için duman pompalama ekipmanı Brawijaya Üniversitesi Tıp Fakültesi Farmakoloji Laboratuvarında tasarlandı ve üniversitemiz kullanımına sunuldu. Kolometrik olarak MDA seviyesi analizi yapıldı. Over folikülü sayısının analizi ve endometriyum kalınlığı hematoksilen eozin boyama ile histopatolojik olarak yapıldı. ELİSA tekniğiyle östradiol seviyesinin analizi yapıldı. Bulgular: Kontroller ile (0.121 ± 0.026) (p> 0.05), sigara dumanına maruz kalan sıçanların over MDA düzeyleri (0.388 ± 0.085) karşılaştırıldı. Sigara dumanına maruz kalan sıçanların over MDA düzeylerinin önemli ölçüde arttığı gözlendi. Çeşitli dozlarda -tokoferol verilen gruplar sigara dumanına (P 0.05) gözlemlenen rakamlara ulaşılmasını sağladı. İlk doz -tokoferol uygulanan gruptaki (3.8 ± 1.095) birincil folikül sayısı, kontrol grubuyla karşılaştırıldı (3.8 ± 1.095) (P> 0.05). Tokoferol grubunda İlk doz (5,2 ± 1,304) ve ikinci doz (5.4 ± 0.548) sekonder folikül sayıları kontrol grubuna (3.6 ± 0.548) (p 0.05) seviyesine ulaştığı gözlendi. Sigara dumanına maruz bırakılan ve bırakılmayan gruplar (600,265 ± 76,563) (p 0.05) seviyelerine ulaştı. Sonuç: Yukarıda belirtilen bu sonuçlara göre, -tokoferol nedeniyle azalmış MDA düzeyleri, primer ve sekonder folikül artışlarının yanı sıra sigara dumanına maruz kalmayla östradiol hormon, oksidatif hasarı ve overlerin fonksiyon kaybını önleyebilir sonucuna varıldı. Aynı zamanda endometriyum kalınlığıda artış gösterdi.

Published
2014

27. Ekstrak ethanol pegagan (Centella asiatica) meningkatkan osifikasi tulang dan panjang badan larva zebrafish (Danio rerio) model stunting usia 9 hari pasca fertilisasi

Author

Zahara, Evi, primary, Nuraenah, Een, additional, Yuliyani, Tri, additional, Darwitri, Darwitri, additional, Khotimah, Husnul, additional, Kalsum, Umi, additional, Wiyasa, I Wayan Arsana, additional, Ramli, Nurlaili, additional, Al Rahmad, Agus Hendra, additional, and Ali, Mohammad Muljohadi, additional

Published
2018
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29. Effect of casein supplementation on HBD-1, Th-17 and IL-22 in a preterm Rattus norvegicus chorioamnionitis model.

Author

Paraton, Hari, Aulanni'am, Aulanni'am, Wiyasa, I. Wayan Arsana, Hariani, Alifah Wahyu, Widjiati, and Prawiro, Sumarno Reto

Subjects
*RATTUS norvegicus, *CHORIOAMNIONITIS, *CASEINS, *DIETARY supplements, *BODY weight, *LIPOPOLYSACCHARIDES
Abstract

Purpose: To investigate casein's role in the expressions of human beta-defensin (HBD)-1, T helper subset (Th)-17, and interleukin (IL)-22 in preterm Rattus norvegicus (RNs). Methods: Twenty-four healthy, first-time pregnant RNs were divided into three groups (G). Group 1 (G1) received regular casein (R) 0.4 g/RN/day, while G2 and G3 received casein supplementation (S) 2 g/RN/day. Each RN was given 10 g of food and drank 10 mL. On day 10, a single intraperitoneal lipopolysaccharide (LPS) was injected with 100 g/kg body weight. Lipopolysaccharide was injected into G3 as a premature model with chorioamnionitis. Histopathological examination was carried out to measure the expression of HBD-1, Th-17, and IL-22 using methyl green counterstaining. Chorioamnion specimens were taken and fixed in 4 % paraformaldehyde, and then evaluated by immunohistochemical (IHC) procedures to obtain immunoreactive score (IMS). Results: The IMS expression of HBD-1 in G1 was 1.187 ± 0.372, while G3 was 9.562 ± 2.351 (p < 0.05). Th-17 expression were G1: 2.062 ± 0.140, G2: 6.122 ± 1.347, and G3: 9.112 ± 2.019 (p < 0.05). Furthermore, IL-22 expressions were G1: 1.825 ± 0.468, G2: 4.700 ± 1.614, and G3: 7.800 ± 1.669 (p < 0.05). Conclusion: Casein supplementation increases HBD-1, Th-17, and IL-22 expression in preterm RN and in the LPS-induced RN chorioamnionitis model. The increase in HBD-1, Th-17, and IL-22 indicate that casein plays a role in the protection and control of chorioamnionitis tissue inflammation. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Rodamin B, Oksidatif Stress Aracılığı ile Ovarian Toksisitesi

Author

MARYANTİ, Syiska Atik, SUCİATİ, Siti, WAHYUNİ, Endang Sri, SANTOSO, Sanarto, and WİYASA, İ Wayan Arsana

Subjects
Medicine, Boya,Toksisite,ovarian,endometriyum,oksidatif stres, Dye,toxicity,ovarium,endometrium,oxidative stress, Tıp
Abstract

Objective: The purpose of this study was to analyze the effects of exposure to rhodamine B on ovarian oxidative stress, ovarian follicles, hormone 17-estradiol and thickness of endometrium. Methods: A total of 28 female rats were divided into four groups consisting of control; groups treated with rhodamine B at doses of 4.5; 9, and 18 milligram/200 gram body weight. Rhodamine B was administered orally for 36 days with the probe. Analysis of MDA level was done spectrophotometrically. Analysis of the number of ovarian follicles and thickness of endometrium was done histopathologically by hematoxylin eosin staining. Analysis of 17-estradiol level was done by ELISA. Results: Rhodamine B administered in different doses in female rats can increase ovarian MDA levels significantly than the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce the number of primary, secondary, and De Graaf follicles significantly compared to the control (P 0.05). Administration of rhodamine B of the second and third doses in female rats can reduce 17-estradiol level significantly compared to the control (P 0.05). The administration of rhodamine B could reduce thickness of endometrium significantly compared to the control (P 0.05). Conclusion: It was concluded that administration of rhodamine B triggered ovarian toxicity through oxidative stress, a decrease in the number of follicles, and decreased level of 17-estradiol which ultimately lowered the thickness of endometrium., Amaç: Çalışmanın amacı, rodamin B"ye maruziyetin, ovarian oksidatif stress, ovarian folikül, 17β-Estradiol hormonu ve endomerium kalınlığı üzerindeki etkisini araştırmak. Materyal Metod: Toplam 28 dişi sıçan kontrol grubu ile birlikte 4 gruba ayrılmıştır. Gruplar 4.5, 9 ve 18 miligram/200 gram vücut ağırlığı olacak şekilde rodamin B ile muamele edilmişir.Rodamin B, 36 gün boyunca probla birlikte oral olarak uygulanmıştır. MDA seviyelerinin analizi spektrofotometrik olarak yapılmıştır. Ovarian folikül sayısı ve endometrium kalınlığı analizleri histopatolojik olarak hematoksilin eozin boyama ile yapılmıştır. 17β-Estradiol seviyesi ELISA ile ölçülmüştür. Bulgular: Dişi sıçanlara farklı dozlarda Rodamin B uygulanması, ovarian MDA seviyelerini kontrollere gore anlamlı şekilde arttırmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozları primer, sekonder ve De Graaf folikül sayısını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Dişi sıçanlarda rodamin B"nin 2. ve 3. dozlarının uygulanması 17β-estradiol seviyesini kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Rodamin B uygulanması endometrium kalınlığını kontrollerle karşılaştırıldığında anlamlı olarak azaltmıştır (P < 0.05). Sonuç: Rodamin B uygulamasının, oksidatif stress aracılığı ile ovarian toksisitesini tetiklediği, folikül sayısını azalttığı ve, 17β-Estradiol seviyesini azaltalarak endometriyum kalınlığını düşürdüğü sonucuna varılmıştır.

Published
2014

31. Sigara Dumanına Maruz Kalan Dişi Sıçanlarda Alfa-Tocopherol'ün Endometrial Uterin Vaskülarizasyonu, Rahim Serviks Oksidatif Stres ve Proliferasyon Üzerine Etkisi

Author

PURBA, Agnes Erna Taulina, ARİTONANG, Juneris, NURDİANA, Nurdiana, SOEHARTO, Setyawati, and WİYASA, İ Wayan Arsana

Subjects
lipid soluble antioxidant, oxidative stress,angiogenesis, reproduction, Health Care Sciences and Services, Sağlık Bilimleri ve Hizmetleri, Lipid çözünürantioksidan,oksidatif stres,anjiyogenez,üreme
Abstract

Purpose: This study aimed toknow whether administration of -tocopherolable to increase uterine endometrial vascularization, inhibit uterine cervix oxidative stress, and augment cervical proliferation in female rats exposed to tobacco smoke. Material and Methods:Twenty five, 8 weeks old female Wistar rats (Rattusnorvegicus) were divided into 5 groups (n=5 each), including control group (non exposure group); the rats were exposed to tobacco smoke (CS); the rats were exposed to tobacco smoke and treated with -tocopherol at dose of 100 mg/kg/day (CS + AT1); rats were exposed to tobacco smoke and treated with -tocopherol at dose of 200mg/kg/day (CS + AT2); rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 400 mg/kg/day (CS + AT3). The expression of endometrial VEGF was observed by immunohistochemical staining using anti-VEGF primary antibody and biotinylated universal secondary antibody. MDA levels were measured colorimetrically. The number of spiralis arterioles and proliferation of uterine cervix epithelial cells were counted histologically withhematoxilin eosin staining. This research has been approved by research ethics committee Faculty of Medicine University of Brawijaya, Malang, Indonesia (Number 477/EC/KEPK-S2-KB/10/2013). Results: Tobacco smoke significantly lowered VEGF level, the number of spiralis arterioles, anduterine cervix epithelial cells compared with control group (P < 0.05). Administration of -tocopherol in different doses significantly increasethe VEGF level to reachs the level in the control group (P > 0.05). Second and third doses of -tocopherolsignificantly increased the number of spiralis arterioles compared with CS group (P < 0.05), to reached the similar level in the control group (P > 0.05).Exposure to tobacco smoke in rats can increase uterine cervix MDA level significantly compared to the control (P< 0.05). The -tocopherol in various doses cansignificantly reduce MDA level compared to the CS (P 0.05). Conclusion:Administration of -tocopherolcan increase the vascularization in endometrial uterine of rats exposed to tobacco smoke. Besides, -tocopherolinhibit uterine cervix oxidative stress, but can"t induces the proliferation of uterine cervix epithelial cells., Amaç: Bu çalışma -tocopherol uygulamasının uterin endometrium vaskülarizasyonu arttırmada, uterin servik soksidatif stresinin hibe edilmesi ve sigara dumanına maruz kalan dişi sıçanlarda servikal çoğalmanın artırılabileceğinin mümkün olup olmadığını öğrenmeyi amaçlamıştır. Materyal ve Metod: Yirmibeş adet, 8 haftalık dişi Wistar sıçanı (Rattusnorvegicus) kontrol grubu (maruz bırakılmamış grup) dahil 5 gruba (n=5) ayrılmıştır; sigara dumanına (CS) maruz bırakılmış sıçanlar; sigara dumanına maruz bırakılmış ve 100mg/kg/gün dozluk (CS+AT1) -tocopherol"aile muamele edilmiş sıçanlar; sigara dumanına maruz bırakılmış ve 200mg/kg/gündozluk (CS+AT2) -tocopherolile e muamele edilmiş sıçanlar; sigara dumanına maruz bırakılmış ve 400mg/kg/gündozluk (CS+AT3) -tocopherolile e muamele edilmiş sıçanlar. Endometrial VEGF"nin ekspresyonu, anti-VEGF, primer antikor vebiyotinlenmiş genel sekonderantikor kullanılarak immunohistokimyasal boyama ile gözlenmiştir. MDA düzeyleri kolorimetrik olarak ölçülmüştür. Spiral arterlerin sayısı ve Rahim serviks epitel hücrelerin çoğalması hematoksiline ozin boyalarla histolojik olarak sayılmıştır. Bu araştırma Endonezya Malang"daki (No 477/EC/KEPK-S2-KB/10/2013) Brawijaya Üniversitesi Tıp Fakültesi"nin araştırma etik komitesi tarafından onaylanmıştır. Bulgular: Sigara dumanı control grubuyla karşılaştırıldığında özellikle VEGF seviyesini, spiral arterlerin ve uterin serviks epitel hücrelerinin sayısını düşürmüştür (P0.05).a-tocopherol"ün İkinci ve üçüncü dozları CS grubuna kıyasla (P0.05). Sıçanlarda sigara dumanına maruz kalmanın serviks MDA düzeyini control grubuna(P

Published
2014

36. Effects of ethanolic extract of Moringa oleifera leaves from West Nusa Tenggara on malondialdehyde level and histological placental labyrinth zone of pregnant Rattus norvegicus exposed to cigarette smoke.

Author

Mutianingsih, Rosa, Kalsum, Umi, Wiyasa, I. Wayan Arsana, and Paundralingga, Obed T. K.

Subjects
*RATTUS norvegicus, *CIGARETTE smoke, *MORINGA oleifera, *LEAVES, *EXTRACTS, *THERAPEUTICS
Abstract

Objective: The aim of this study was to observe the effects of ethanolic extract of Moringa oleifera varietas West Nusa Tenggara leaves on malondialdehyde (MDA) level and histological placental labyrinth zone of pregnant Rattus norvegicus exposed to cigarette smoke. Materials and Methods: This was an experimental study with post-test only control group design. Twenty pregnant R. norvegicus were randomly divided into five groups, namely negative control (-), positive control (+), and treatment of ethanolic extract of M. oleifera of 25 mg/kg BB/day (P1), 50 mg/kg BB/day (P2), and 100 mg/kg BB/day (P3). Results: This study showed an ethanolic extract of M. oleifera which showed significant effects on MDA level (P < 0.05). There was no significant effect of ethanolic extract of M. oleifera in various dose on placental labyrinth zone (P > 0.05). Conclusion: Ethanolic extract of M. oleifera decreased MDA level with optimal dose 25 mg/kg BB/day, but no significant effect on placental labyrinth zone of pregnant rats exposed to cigarette smoke at 14 days of pregnancy. [ABSTRACT FROM AUTHOR]

Published
2019

38. Genistein administration increases the level of superoxide dismutase and glutathione peroxidase in the endometriosis mice model: An experimental study.

Author

Sutrisno, Sutrisno, Miryani, Ira, Dwijayasa, Pande Made, Suprobo, Nina Rini, and Wiyasa, I. Wayan Arsana

Subjects
*GLUTATHIONE peroxidase, *SUPEROXIDE dismutase, *GENISTEIN, *ANIMAL disease models, *ENDOMETRIOSIS
Abstract

Background: Endometriosis and infertility are caused by reactive oxygen species or free radicals, which promote endometrial cell growth and adhesion in the peritoneal cavity. Genistein has been proven to protect cells against reactive oxygen species by scavenging free radicals and decreasing the expression of genes-associated stress responses. Objective: This study was conducted to determine whether genistein also acts as an antioxidant by elevating superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the peritoneal fluid of the endometriosis mice model. Materials and Methods: This experimental study involved 32 healthy female mice (Mus musculus), aged between 2-3 months and weighing 20-30 gr. They were divided into negative control group (healthy mice without genistein), endometriosis group (endometriosis mice without genistein), treatment group that was given different doses of genistein, that is, 0.13; 0.26; 0.52; 0.78; 1.04; and 1.3 mg/day (n = 4/each). SOD level in the peritoneal fluid was measured using the quantitative colorimetric determination method, and a colorimetric assay measured the GPx levels. Results: Results showed that the endometriosis model has lower SOD and GPx levels than the control group. The administration of genistein significantly normalized these changes. Genistein significantly increased SOD levels in the 0.13 mg and 0.26 mg treatment groups. Genistein also increased GPx levels significantly in all treatment groups. Conclusion: Genistein increases SOD and GPx levels in the peritoneal fluid of an endometriosis mice model, and the change is dose-dependent. [ABSTRACT FROM AUTHOR]

Published
2022
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