27 results on '"Wolfgang, D. R."'
Search Results
2. A mathematical model of the dynamics of Salmonella Cerro infection in a US dairy herd
- Author
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CHAPAGAI0N, P. P., VAN KESSEL, J. S., KARNS, J. S., WOLFGANG, D. R., HOVINGH, E., NELEN, K. A., SCHUKKEN, Y. H., and GROHN, Y. T.
- Published
- 2008
3. Longitudinal relationship between fecal culture, fecal quantitative PCR, and milk ELISA in Mycobacterium avium ssp paratuberculosis-infected cows from low-prevalence dairy herds
- Author
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Beaver, A., Sweeney, R.W., Hovingh, S.E., Wolfgang, D. R., Grohn, Y. T., Schukken, Y. H., LS Theoretische Epidemiologie, dFAH AVR, LS Theoretische Epidemiologie, and dFAH AVR
- Subjects
0301 basic medicine ,Veterinary medicine ,milk ELISA ,Kwantitatieve Veterinaire Epidemiologie ,Paratuberculosis ,Polymerase Chain Reaction ,Milk elisa ,0403 veterinary science ,Feces ,New England ,Prevalence ,Udder ,biology ,food and beverages ,04 agricultural and veterinary sciences ,Mycobacterium avium subsp. paratuberculosis ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,Milk ,Female ,Fecal culture ,fecal culture ,040301 veterinary sciences ,fecal quantitative PCR ,Cattle Diseases ,Enzyme-Linked Immunosorbent Assay ,Mycobacterium avium ssp paratuberculosis ,Mycobacterium avium ssp. paratuberculosis ,03 medical and health sciences ,Genetics ,medicine ,Bulk tank ,Animals ,Milk ELISA ,Quantitative Veterinary Epidemiology ,medicine.disease ,biology.organism_classification ,Fecal quantitative PCR ,030104 developmental biology ,Cross-Sectional Studies ,Immunology ,Herd ,WIAS ,Linear Models ,Animal Science and Zoology ,Cattle ,Johne's disease ,Food Science ,Mycobacterium - Abstract
Mycobacterium avium ssp. paratuberculosis (MAP), the causative agent of ruminant Johne's disease, presents a particular challenge with regard to infection mitigation on dairy farms. Diagnostic testing strategies to identify and quantify MAP and associated antibodies are imperfect, and certain facets of the relationship between diagnostic tests remain to be explored. Additional repeated-measures data from known infected animals are needed to complement the body of cross-sectional research on Johne's disease-testing methods. Statistical models that accurately account for multiple diagnostic results while adjusting for the effects of individual animals and herds over time can provide a more detailed understanding of the interplay between diagnostic outcomes. Further, test results may be considered as continuous wherever possible so as to avoid the information loss associated with dichotomization. To achieve a broader understanding of the relationship between diagnostic tests, we collected a large number of repeated fecal and milk samples from 14 infected cows, in addition to bulk milk samples, from 2 low-prevalence dairy herds in the northeast United States. Predominately through the use of mixed linear modeling, we identified strong associations between milk ELISA optical density, fecal quantitative PCR, and fecal culture in individual animals while concurrently adjusting for variables that could alter these relationships. Notably, we uncovered subtleties in the predictive abilities of fecal shedding level on milk ELISA results, with animals categorized as disease progressors reaching higher ELISA optical density levels. Moreover, we observed that spikes in fecal shedding could predict subsequent high ELISA values up to 2 mo later. We also investigated the presence of MAP in individual milk samples via PCR and noted an association between poor udder hygiene and MAP positivity in milk, suggesting some level of environmental contamination. The paucity of positive milk samples and the complete absence of detectable MAP in the bulk tank throughout the study period indicate that contamination of milk with MAP may not be of chief concern in low-prevalence herds. An enhanced understanding of the interrelationships between diagnostic tests can only benefit the development of testing strategies and objectives, which in turn may lessen MAP infection prevalence in dairy herds.
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- 2017
4. The effects of progressing and nonprogressing Mycobacterium avium ssp. paratuberculosis infection on milk production in dairy cows
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Smith, Rebecca L., Gröhn, Y. T., Pradhan, A. K., Whitlock, R. H., Van Kessel, J. S., Smith, J. M., Wolfgang, D. R., Schukken, Y. H., LS Theoretische Epidemiologie, and dFAH AVR
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Disease status ,040301 veterinary sciences ,Paratuberculosis ,Cattle Diseases ,Article ,0403 veterinary science ,Feces ,Animal science ,New England ,Milk production ,Mixed linear model ,medicine ,Genetics ,Animals ,Positive test ,biology ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,biology.organism_classification ,medicine.disease ,040201 dairy & animal science ,Mycobacterium avium subsp. paratuberculosis ,Milk ,Herd ,Linear Models ,Cattle ,Female ,Animal Science and Zoology ,Johne's disease ,Mycobacterium ,Food Science - Abstract
Longitudinal data from 3 commercial dairy herds in the northeast United States, collected from 2004 to 2011, were analyzed to determine the effect of Mycobacterium avium ssp. paratuberculosis (MAP) infection status and progression path on milk production. Disease status, as indicated by MAP test results, was determined through quarterly ELISA serum testing, biannual fecal culture, and culture of tissues and feces at slaughter. Milk production data were collected from the Dairy Herd Information Association. Animals with positive MAP test results were categorized, based on test results over the full course of the study, as high path (at least one high-positive culture) or low path (at least one positive culture or ELISA). The cumulative numbers of positive ELISA and culture results were recorded. The effects of both MAP infection path, status, and number of positive tests on milk production were analyzed using a mixed linear model with an autocorrelation random effect structure. Low- and high-path animals produced more milk before their first positive test than always-negative animals, especially high-path animals. Although mean production decreased after a first positive test, low-path animals were shown to recover some productivity. High-path animals continued to exhibit a decrease in milk production, especially after their first high-positive fecal culture. These results show that not all animals that test positive for MAP will have long-term production losses. Milk production decreased significantly with each additional positive test. Ultimately, production loss appeared to be a function of MAP infection progression.
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- 2016
5. 266 Prevalence and risk factors for antimicrobial resistance on U.S. dairy operations
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Cao, H., primary, Pradhan, A. K., additional, Karns, J. S., additional, Wolfgang, D. R., additional, Hovingh, E., additional, Vinyard, B. T., additional, and Van Kessel, J. A. S., additional
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- 2017
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6. Identification of novel small molecule antimicrobials targeting Mycoplasma bovis
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Soehnlen, M. K., primary, Tran, M. A., additional, Lysczek, H. R., additional, Wolfgang, D. R., additional, and Jayarao, B. M., additional
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- 2011
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7. Evaluating AIDS prevention for men having sex with men: the west European experience
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Michael Pollak, Wolfgang D�r, Michel Vincineau, Michel Perreault, Richard Prusa, Jan Fouchard, Michael Bochow, Olli Stalstr�m, Rainer Herrn, Peter Davies, Gregory Vallianatos, Charles Kerrigan, Francesco Allegrini, Hartmut Sasse, Rob Tielman, Annick Prieur, Oscar Guasch Andreu, Benny Henriksson, Jean Blaise Masur, and Marie-Ange Schiltz
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Research design ,Gerontology ,Adult ,Male ,medicine.medical_specialty ,Health Knowledge, Attitudes, Practice ,European community ,media_common.quotation_subject ,Sexual Behavior ,Population ,Health knowledge ,Sampling Studies ,Acquired immunodeficiency syndrome (AIDS) ,medicine ,Humans ,Men having sex with men ,Homosexuality ,education ,Health Education ,media_common ,education.field_of_study ,Acquired Immunodeficiency Syndrome ,Public health ,Public Health, Environmental and Occupational Health ,AIDS Serodiagnosis ,medicine.disease ,Europe ,Research Design ,Psychology - Abstract
This article presents the major results of a Concerted Action of the European Community on “Assessing AIDS-prevention” concerning the male homo- and bisexual population. It discusses the methodologies and results of research projects undertaken in this area in the 1980's.
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- 1994
8. A mathematical model of the dynamics of Salmonella Cerro infection in a US dairy herd
- Author
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CHAPAGAIN, P. P., primary, VAN KESSEL, J. S., additional, KARNS, J. S., additional, WOLFGANG, D. R., additional, HOVINGH, E., additional, NELEN, K. A., additional, SCHUKKEN, Y. H., additional, and GROHN, Y. T., additional
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- 2007
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9. Environmental Sampling To Predict Fecal Prevalence of Salmonella in an Intensively Monitored Dairy Herd.
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VAN KESSEL, J. S., KARNS, J. S., WOLFGANG, D. R., HOVINGH, E., JAYARAO, B. M., VAN TASSELL, C. P., and SCHUKKEN, Y. H.
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ENVIRONMENTAL sampling ,SALMONELLA ,PATHOGENIC microorganisms ,FECES ,MICROBIOLOGY ,DAIRY cattle ,MILK quality - Abstract
Although dairy cattle are known reservoirs for salmonellae, cattle that are shedding this organism are often asymptomatic and difficult to identify. A dairy herd that was experiencing a sustained, subclinical outbreak of Salmonella enterica subsp. enterica Cerro was monitored for 2 years. Fecal samples from the lactating cows were collected every 6 to 8 weeks and tested for the presence of Salmonella. Fecal prevalence of Salmonella fluctuated throughout the observation period and ranged from 8 to 88%. Manure composites and water trough samples were collected along with the fecal samples, and bulk milk and milk filters were cultured for the presence of Salmonella on a weekly basis. Over 90% of the manure composites--representing high-animal-traffic areas--were positive at each sampling. Salmonella was detected in 11% of milk samples and in 66% of the milk filters. Results of weekly bulk milk quality testing (i.e., bulk tank somatic cell score, standard plate count, preliminary incubation count) were typically well within acceptable ranges. Milk quality variables had low correlations with herd Salmonella fecal prevalence. When observed over time, sampling period average prevalence of Salmonella in milk filters closely paralleled fecal prevalence of Salmonella in the herd. Based on results of this study, milk filters appear to be an effective method for monitoring shedding prevalence at the herd level. In-line filter testing is also a more sensitive measure of Salmonella, and perhaps other pathogens, in raw milk than testing the milk alone. [ABSTRACT FROM AUTHOR]
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- 2008
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10. Prevalence and risk factors for antimicrobial resistance on U.S. dairy operations.
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Cao, H., Pradhan, A. K., Karns, J. S., Wolfgang, D. R., Hovingh, E., Vinyard, B. T., and Van Kessel, J. A. S.
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DRUG resistance in bacteria ,DAIRY farms ,LOGISTIC regression analysis - Abstract
Antimicrobial resistance has become a major public health concern, and animal agriculture is often implicated as a source of resistant bacteria. The primary objective of this study was to determine prevalence of antimicrobial resistance in Salmonella and E. coli from healthy animals on dairy farms in Pennsylvania. Confidence intervals (CI) of farm prevalence were calculated for resistance, and the comparisons between different animal groups were adjusted for within-farm clustering. The secondary objective was to identify potential risk factors associated with antimicrobial resistance. In particular, we investigated the correlations between previous antimicrobial use and current resistance in E. coli using multiple logistic regression models. A total of 444 composite manure samples were collected from 80 herds in Pennsylvania. Four age and production groups were included: pre-weaned calves, post-weaned calves, dry cows, and lactating cows. E. coli (n = 2370) and Salmonella (n = 1095), when isolated from the manure composite samples (n = 444), were screened for resistance on antimicrobial supplemented agar. Isolates of interest were further assayed against 14 antimicrobials using a broth microdilution method. Salmonella was isolated from at least one composite manure sample from 51 (67%) farms, with 99% being pan-susceptible, and was detected more frequently in lactating cows and dry cows than pre- and post-weaned calves. The most prevalent Salmonella serogroups were K, C1, and C2. Among the E. coli isolates, resistance was more frequently detected in pre-weaned and post-weaned calves than in dry and lactating cows for all 14 antimicrobials tested (P < 0.05). Multi-drug resistance (resistant to ≥3 antimicrobial classes) in E. coli was detected in 66 farms (83%) and was detected more often in pre-weaned calves (P < 0.05). The blaCTX-M and bla
CMY genes were found in cephalosporin-resistant E. coli from 5% and 35% of the farms surveyed, respectively. A number of risk factors, including antimicrobial use, were found to be associated with an increased likelihood of resistance in E. coli, and these associations were not limited to the same antimicrobial classes. The results of this study showed dairy farms, especially pre-weaned calves and their environment, are significant reservoirs for antimicrobial resistance, and antimicrobial use may aggravate the resistance problem. [ABSTRACT FROM AUTHOR]- Published
- 2017
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11. Environmental sampling to predict fecal prevalence of Salmonella in an intensively monitored dairy herd
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Kessel, J. S., Karns, J. S., Wolfgang, D. R., Hovingh, E., Jayarao, B. M., Tassell, C. P., and Ynte Schukken
12. A long-term, sub-clinical, outbreak of Salmonella enterica subsp. enterica Cerro in a Pennsylvania dairy herd.
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Van Kessel, J. S., Karns, J. S., Wolfgang, D. R., Hovingh, E., and Schukken, Y. H.
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SALMONELLA enterica ,SALMONELLA ,ANIMAL herds ,FOOD pathogens ,CULTURED milk ,ENVIRONMENTAL sampling ,SALMONELLA diseases - Abstract
Salmonella is a prominent foodborne pathogen in the US and dairy cattle have frequently been identified as reservoirs for this organism. Here we describe a long-term outbreak of Salmonella enterica subsp. enterica Cerro that was detected in a Pennsylvania dairy herd during routine monitoring as part of a longitudinal study of several dairy herds. An initial analysis of individual fecal grab samples from the milking herd indicated a very low prevalence of Salmonella (1/102) and Salmonella was not detected in 10 environmental samples. In a subsequent sampling 6 mo later, Salmonella was isolated from 43.5% (47/108) of the cows and 40% of collected environmental samples (10/25). The Salmonella isolated in the first sampling was serovar Typhimurium var. Copenhagen, however isolates from the second herd sampling were serovars Cerro (91.5%) and Kentucky (8.5%). Sporadic environmental sampling between these initial complete milking herd samplings yielded serotypes Kentucky, Typhimurium var. Copenhagen, and Cerro and clinical salmonellosis was observed in several cows shedding Typhimurium var. Copenhagen. The herd was sampled nine additional times over the next 14 mo and fecal prevalence of Salmonella ranged from 8.4% to 89%. Prevalence remained high (58 to 69%) over the following 8 mo, showed evidence of decline over the next 3 mo, and then increased again to 89%. Cerro was the only serotype isolated during this period. Bulk milk was cultured weekly during this 22-mo period and Salmonella was isolated from 5.3% (5/94) of milk samples. For the last 14 mo of the study, milk filters were also cultured for Salmonella on a weekly basis and 65% (37/57) of the filters tested positive. There were no significant health or production concerns in the herd during this outbreak. Based on these observations we conclude that sub-clinical Salmonella enterica subsp. enterica Cerro infection can be persistent in a milking herd. [ABSTRACT FROM AUTHOR]
- Published
- 2006
13. Identification and implications of MAP supershedders.
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Hovingh, E., Whitlock, R. H., Sweeney, R. W., Fyock, T., Wolfgang, D. R., Smith, J., Schukken, Y. H., and Van Kessel, J. S.
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FECAL contamination ,PARATUBERCULOSIS ,MYCOBACTERIUM avium paratuberculosis ,EGG yolk ,CATTLE manure ,ENVIRONMENTAL exposure ,LACTATION in cattle - Abstract
The objective of this research was to determine the range of colonyforming units (cfu) per gram of Mycobacterium avium paratuberculosis (MAP) in manure from cows previously classified as heavy shedders, and the role of these animals in Johne's Disease (JD) in dairy herds. Cattle are typically classified as light, moderate or heavy shedders based on the number of MAP colonies seen on Herrold's egg yolk media (HEYM). Laboratories routinely report the number of cfu up to 70 per HEYM tube. (Seventy cfu corresponds to an estimated 1,470 MAP cfu per gram of manure.) Counts above 70 are reported as 'too numerous to count' and these cows are classified as heavy shedders. Preliminary investigation found that 1:100 and 1:1,000 dilutions of feces from heavy shedders were appropriate for enumerating the actual concentration of MAP in these samples. Subsequently, more than 200 fecal samples from animals currently identified as heavy shedders were diluted and cultured on HEYM. Supershedders (SS) were defined as animals that had manure with more than 10,000 cfu/gm. Culture results on diluted samples demonstrated that a substantial proportion of heavy shedders were SS, with some animals exceeding 1,000,000 MAP cfu/gm. Whole herd cultures from 4 herds found that 10% to 15% of all culture positives were SS. Not all of these SS cattle demonstrated clinical signs of JD at the time of sampling. It was calculated that SS cattle shedding the highest concentration of MAP were shedding more cfu than 2,000 moderate or 20,000 light shedders. Subsequent culture in one herd after the removal of a SS identified up to 15 animals that were potential false positives on the previous culture. This study has demonstrated the previously undocumented presence of MAP supershedders. The results suggest that SS contribute disproportionately to the herd environmental bioburden and the exposure of uninfected animals to MAP organisms. The data also suggest that the bioburden in herds with a SS present may contribute to herdmates being incorrectly identified as culture positive. Identification and removal of SS animals is likely to be a critical component of a JD control and management program. [ABSTRACT FROM AUTHOR]
- Published
- 2006
14. Efficacy of on-farm use of ultraviolet light for inactivation of bacteria in milk for calves.
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Gelsinger, S. L., Heinrichs, A. J., Jones, C. M., van Saun, R. J., Wolfgang, D. R., Burns, C. M., and Lysczek, H. R.
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ULTRAVIOLET radiation , *BACTERIAL inactivation , *MILK microbiology , *CATTLE feeding & feeds , *CALVES , *CATTLE nutrition , *GRAM-negative bacteria , *STREPTOCOCCUS , *STAPHYLOCOCCUS - Abstract
Ultraviolet light is being employed for bacterial inactivation in milk for calves; however, limited evidence is available to support the claim that UV light effectively inactivates bacteria found in milk. Thus, the objective of this observational study was to investigate the efficacy of on-farm UV light treatment in reducing bacteria populations in waste milk used for feeding calves. Samples of nonsaleable milk were collected from 9 Pennsylvania herds, twice daily for 15 d, both before and after UV light treatment (n = 60 samples per farm), and analyzed for standard plate count, coliforms, non-coliform, gram-negative bacteria, environmental and contagious streptococci, coagulase-negative staphylococci, Streptococcus agalactiae, Staphylococcus aureus count, and total solids percentage, and log reduction and percentage log reduction were calculated. Data were analyzed using the mixed procedure in SAS. In all bacteria types, samples collected after UV treatment contained significantly fewer bacteria compared with samples collected before UV treatment. Weighted least squares means for log reduction (percentage log reduction) were 1.34 (29%), 1.27 (58%), 1.48 (53%), 1.85 (55%), 1.37 (72%), 1.92 (63%), 1.07 (33%), and 1.67 (82%) for standard plate count, coliforms, noncoliform, gram-negative bacteria, environmental and contagious streptococci, Strep. agalactiae, coagulase-negative staphylococci, and Staph. aureus, respectively. A percentage log reduction greater than 50% was achieved in 6 of 8 bacteria types, and 43 and 94% of samples collected after UV treatment met recommended bacterial standards for milk for feeding calves. Based on these results, UV light treatment may be effective for some, but not all bacteria types found in nonsaleable waste milk. Thus, farmers should take into account the bacteria types that may need to be reduced when considering the purchase of a UV-treatment system. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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15. Epidemiology of Mycoplasma bovis in Pennsylvania veal calves.
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Soehnlen, M. K., Aydin, A., Murthy, K. S., Lengerich, E. J., Hattel, A. L., Houser, B. A., Fenton, G. D., Lysczek, H. R., Burns, C. M., Townsend, A. M., Brooks, J. W., Wolfgang, D. R., and Jayarao, B. M.
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MYCOPLASMA diseases , *CALVES , *COWS , *CATTLE , *VACCINES - Abstract
The objective of the study was to estimate the prevalence and incidence of Mycoplasma bovis, a common cause of pneumonia, in veal calves. Using simple random sampling, 252 calves from 4 veal herds located in central Pennsylvania were selected and longitudinally followed for monthly collection of nasal swabs. Bronchial swabs and lung lesions were collected at the slaughterhouse. Nasal, bronchial, and lung lesion swabs were cultured for bacterial respiratory pathogens. Ninety lung lesions were identified, of which 41.1, 1.1, 1.1, 7.8, and 4.4% were culture positive for M. bovis alone, Pasteurella multocida alone, Mannheimia haemolytica alone, M. bovis and P. multocida co-infection, and M. bovis and M. haemolytica co-infection, respectively. The data indicate that potential interventions, such as therapeutics, vaccines, or management control measures, would be most effective before 50 d of age based upon the cumulative incidence of colonization. [ABSTRACT FROM AUTHOR]
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- 2012
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16. Molecular epidemiological analysis of Mycoplasma bovis isolates from the Pennsylvania Animal Diagnostic Laboratory showing genetic diversity.
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Soehnlen, M. K., Kariyawasam, S., Lumadue, J. A., Pierre, T. A., Wolfgang, D. R., and Jayarao, B. M.
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CATTLE diseases , *MOLECULAR epidemiology , *MYCOPLASMA diseases , *POLYMERASE chain reaction , *GENETIC polymorphisms , *VETERINARY medicine - Published
- 2011
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17. Effect of Johne's disease status on reproduction and culling in dairy cattle.
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Smith, R. L., Strawderman, R. L., Schukken, Y. H., Wells, S. J., Pradhan, A. K., Espejo, L. A., Whitlock, R. H., Van Kessel, J. S., Smith, J. M., Wolfgang, D. R., and Gröhn, Y. T.
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MYCOBACTERIUM avium paratuberculosis , *PARATUBERCULOSIS , *ENZYME-linked immunosorbent assay , *LIVESTOCK diseases , *DAIRY cattle , *COW diseases , *REGRESSION analysis , *CATTLE parturition - Abstract
Among the costs attributed to Mycobacterium avium ssp. paratuberculosis (MAP) infection in dairy cattle, the effects on reproduction and culling are the least documented. To estimate the cost of MAP infections and Johne's disease in a dairy herd, the rates of calving and culling were calculated for cows in each stage of MAP infection relative to uninfected cows. Data from 6 commercial dairy herds, consisting of 2,818 cows with 2,754 calvings and 1,483 cullings, were used for analysis. Every cow in each study herd was tested regularly for MAP, and herds were followed for between 4 and 7 yr. An ordinal categorical variable for Johne's disease status [test-negative, low-positive (low-shedding or ELISA-positive only), or high-shedding] was defined as a time-dependent variable for all cows with at least 1 positive test result or 2 negative test results. A Cox regression model, stratified on herd and controlling for the time-dependent infection variable, was used to analyze time to culling. Nonshedding animals were significantly less likely to be culled in comparison with animals in the low-shedding or ELISA-positive category, and highshedding animals had nonsignificantly higher culling rates than low-shedding or ELISA-positive animals. Time to calving was analyzed using a proportional rates model, an analog to the Andersen-Gill regression model suitable for recurrent event data, stratifying on herd and weighted to adjust for the dependent censoring caused by the culling effects described above. Highshedding animals had lower calving rates in comparison with low-shedding or ELISA-positive animals, which tended to have higher calving rates than test-negative animals. [ABSTRACT FROM AUTHOR]
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- 2010
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18. A longitudinal study on the impact of Johne's disease status on milk production in individual cows.
- Author
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Smith, R. L., Grohn, Y. T., Pradhan, A. K., Whitlock, R. H., van Kessel, J. S., Smith, J. M., Wolfgang, D. R., and Schukken, Y. H.
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PARATUBERCULOSIS , *MYCOBACTERIUM avium , *MYCOBACTERIUM , *DAIRY farms , *DAIRY cattle - Abstract
Longitudinal data from 3 commercial dairy herds in the northeast United States were collected from 2004 to 2007. Johne's disease status, as indicated by Mycobacterium avium ssp. paratuberculosis infection levels, was determined through quarterly ELISA serum testing, biannual fecal culture, and culture of tissues at slaughter. Milk production data were collected from the Dairy Herd Improvement Association. The effect of Johne's disease status on milk production was analyzed using a mixed linear model with an autocorrelation random effect structure. Infected animals produced more milk than uninfected cows before they began shedding M. avium ssp. paratuberculosis. Cows infected with M. avium ssp. paratuberculosis had monthly decreases of 0.05 to 1 kg in daily milk production relative to uninfected animals, with greater decreases in progressive disease categories. Animals with fecal culture results of >30 cfu/g produced approximately 4 kg less milk per day compared with uninfected cows. These results will be valuable in calculating the economic effect of Johne's disease. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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19. Dynamics of endemic infectious diseases of animal and human importance on three dairy herds in the northeastern United States.
- Author
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Pradhan, A. K., Van Kessel, J. S., Karns, J. S., Wolfgang, D. R., Hovingh, E., NeIen, K. A., Smith, J. M., Whitlock, R. H., Fyock, T., Ladely, S., Fedorka-Cray, P. J., and Schukken, Y. H.
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PUBLIC health , *HUMAN services , *DAIRY products , *MEAT - Abstract
Endemic infectious diseases in dairy cattle are of significant concern to the industry as well as for public health because of their potential impact on animal and human health, milk and meat production, food safety, and economics. We sought to provide insight into the dynamics of important endemic infectious diseases in 3 northeastern US dairy herds. `Fecal samples from individual cows and various environmental samples from these farms were tested for the presence of major zoonotic pathogens (i.e., Salmonella, Campylobacter, and Listeria) as well as commensal bacteria Escherichia coli and enterococci. Additionally, the presence of Mycobacterium avinin ssp. paratuberculosis was tested in fecal and serum samples from individual cows. Test results and health and reproductive records were maintained in a database, and fecal, plasma, DNA, and tissue samples were kept in a biobank. All bacteria of interest were detected on these farms and their presence was variable both within and between farms. The prevalence of Listeria spp. and L. monocytogenes in individual fecal samples within farm A ranged from 0 to 68.2% and 0 to 25.5%, respectively, over a period of 3 yr. Within farm B, continuous fecal shedding of Salmonella spp. was observed with a prevalence ranging from 8 to 88%; Salmonella Cerro was the predominant serotype. Farm C appeared less contaminated with Salmonella and Listeria, although in the summer of 2005, 50 and 19.2% of fecal samples were positive for Listeria and L. monocytogenes, respectively. The high prevalence of E. coli (89 to 100%), Enterococcus (75 to 100%), and Campylobacter (0 to 81%) in feces suggested they were ubiquitous throughout the farm environment. Fecal culture and ELISA results indicated a low prevalence of Mycobacterium avium ssp. paratuberculosis infection in these farms (0 to 13.6% and 0 to 4.9% for culture-positive and ELISA-positive, respectively), although the occasional presence of high shedders was observed. Results have major implications for food safety and epidemiology by providing a better understanding of infectious disease dynamics on dairy farms. Comprehensive understanding of these infections may lead to better farm management practices and pathogen reduction programs to control and reduce the on-farm contamination of these pathogens and to prevent their further entry into the food-chain. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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20. Guidelines for Monitoring Bulk Tank Milk Somatic Cell and Bacterial Counts.
- Author
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Jayarao, B. M., Pillai, S. R., Sawant, A. A., Wolfgang, D. R., and Hegde, N. V.
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MILK microbiology , *MILK quality , *COMPOSITION of milk , *DAIRY microbiology , *DAIRY industry - Abstract
This study was conducted to establish guidelines for monitoring bulk tank milk somatic cell count and bacterial counts, and to understand the relationship between different bacterial groups that occur in bulk tank milk. One hundred twenty-six dairy farms in 14 counties of Pennsylvania participated, each providing one bulk tank milk sample every 15 d for 2 mo. The 4 bulk tank milk samples from each farm were examined for bulk tank somatic cell count and bacterial counts including standard plate count, preliminary incubation count, laboratory pasteurization count, coagulase-negative staphylococcal count, environmental streptococcal count, coliform count, and gram-negative noncoliform count. The milk samples were also examined for presence of Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma. The bacterial counts of 4 bulk tank milk samples examined over an 8-wk period were averaged and expressed as mean bacterial count per milliliter. The study revealed that an increase in the frequency of isolation of Staphylococcus aureus and Streptococcus agalactiae was significantly associated with an increased bulk tank somatic cell count. Paired correlation analysis showed that there was low correlation between different bacterial counts. Bulk tank milk with low (< 5000 cfu/mL) standard plate count also had a significantly low level of mean bulk tank somatic cell count (< 200,000 cells/mL), preliminary incubation count (< 10,000 cfu/mL), laboratory pasteurization count (< 100 cfu/mL), coagulase-negative staphylococci and environmental streptococcal counts (< 500 cfu/mL), and noncoliform count (< 200 cfu/mL). Coliform count was less likely to be associated with somatic cell or other bacterial counts. Herd size and farm management practices had considerable influence on somatic cell and bacterial counts in bulk tank milk. Dairy herds that used automatic milking detachers, sand as bedding ma terial, dip cups for.. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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- View/download PDF
21. Longitudinal relationship between fecal culture, fecal quantitative PCR, and milk ELISA in Mycobacterium avium ssp. paratuberculosis-infected cows from low-prevalence dairy herds.
- Author
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Beaver A, Sweeney RW, Hovingh E, Wolfgang DR, Gröhn YT, and Schukken YH
- Subjects
- Animals, Cattle, Cattle Diseases prevention & control, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay veterinary, Female, Linear Models, New England, Paratuberculosis prevention & control, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Prevalence, Cattle Diseases diagnosis, Feces microbiology, Milk microbiology, Mycobacterium avium subsp. paratuberculosis isolation & purification, Paratuberculosis diagnosis
- Abstract
Mycobacterium avium ssp. paratuberculosis (MAP), the causative agent of ruminant Johne's disease, presents a particular challenge with regard to infection mitigation on dairy farms. Diagnostic testing strategies to identify and quantify MAP and associated antibodies are imperfect, and certain facets of the relationship between diagnostic tests remain to be explored. Additional repeated-measures data from known infected animals are needed to complement the body of cross-sectional research on Johne's disease-testing methods. Statistical models that accurately account for multiple diagnostic results while adjusting for the effects of individual animals and herds over time can provide a more detailed understanding of the interplay between diagnostic outcomes. Further, test results may be considered as continuous wherever possible so as to avoid the information loss associated with dichotomization. To achieve a broader understanding of the relationship between diagnostic tests, we collected a large number of repeated fecal and milk samples from 14 infected cows, in addition to bulk milk samples, from 2 low-prevalence dairy herds in the northeast United States. Predominately through the use of mixed linear modeling, we identified strong associations between milk ELISA optical density, fecal quantitative PCR, and fecal culture in individual animals while concurrently adjusting for variables that could alter these relationships. Notably, we uncovered subtleties in the predictive abilities of fecal shedding level on milk ELISA results, with animals categorized as disease progressors reaching higher ELISA optical density levels. Moreover, we observed that spikes in fecal shedding could predict subsequent high ELISA values up to 2 mo later. We also investigated the presence of MAP in individual milk samples via PCR and noted an association between poor udder hygiene and MAP positivity in milk, suggesting some level of environmental contamination. The paucity of positive milk samples and the complete absence of detectable MAP in the bulk tank throughout the study period indicate that contamination of milk with MAP may not be of chief concern in low-prevalence herds. An enhanced understanding of the interrelationships between diagnostic tests can only benefit the development of testing strategies and objectives, which in turn may lessen MAP infection prevalence in dairy herds., (Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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22. The effects of progressing and nonprogressing Mycobacterium avium ssp. paratuberculosis infection on milk production in dairy cows.
- Author
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Smith RL, Gröhn YT, Pradhan AK, Whitlock RH, Van Kessel JS, Smith JM, Wolfgang DR, and Schukken YH
- Subjects
- Animals, Cattle, Cattle Diseases microbiology, Feces microbiology, Female, Linear Models, Mycobacterium avium subsp. paratuberculosis isolation & purification, New England epidemiology, Paratuberculosis microbiology, Cattle Diseases physiopathology, Milk metabolism, Mycobacterium avium subsp. paratuberculosis immunology, Paratuberculosis physiopathology
- Abstract
Longitudinal data from 3 commercial dairy herds in the northeast United States, collected from 2004 to 2011, were analyzed to determine the effect of Mycobacterium avium ssp. paratuberculosis (MAP) infection status and progression path on milk production. Disease status, as indicated by MAP test results, was determined through quarterly ELISA serum testing, biannual fecal culture, and culture of tissues and feces at slaughter. Milk production data were collected from the Dairy Herd Information Association. Animals with positive MAP test results were categorized, based on test results over the full course of the study, as high path (at least one high-positive culture) or low path (at least one positive culture or ELISA). The cumulative numbers of positive ELISA and culture results were recorded. The effects of both MAP infection path, status, and number of positive tests on milk production were analyzed using a mixed linear model with an autocorrelation random effect structure. Low- and high-path animals produced more milk before their first positive test than always-negative animals, especially high-path animals. Although mean production decreased after a first positive test, low-path animals were shown to recover some productivity. High-path animals continued to exhibit a decrease in milk production, especially after their first high-positive fecal culture. These results show that not all animals that test positive for MAP will have long-term production losses. Milk production decreased significantly with each additional positive test. Ultimately, production loss appeared to be a function of MAP infection progression., (Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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23. Environmental contamination with Mycobacterium avium subsp. paratuberculosis in endemically infected dairy herds.
- Author
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Smith RL, Schukken YH, Pradhan AK, Smith JM, Whitlock RH, Van Kessel JS, Wolfgang DR, and Grohn YT
- Subjects
- Animals, Cattle, Cattle Diseases epidemiology, Cross-Sectional Studies, Feces microbiology, Female, Linear Models, Logistic Models, Longitudinal Studies, Manure microbiology, New York epidemiology, Paratuberculosis epidemiology, Pennsylvania epidemiology, Population Surveillance, Prevalence, Seasons, Sensitivity and Specificity, Vermont epidemiology, Bacterial Shedding, Cattle Diseases microbiology, Dairying, Environmental Microbiology, Mycobacterium avium subsp. paratuberculosis isolation & purification, Paratuberculosis microbiology
- Abstract
Environmental contamination with Mycobacterium avium subsp. paratuberculosis (MAP) is thought to be one of the primary sources of infection for dairy cattle. The exact link between fecal shedding of MAP by individual cows and environmental contamination levels at the herd level was explored with a cross-sectional analysis of longitudinally collected samples on 3 dairy farms. Composite samples from multiple environmental sites in 3 commercial dairy herds in the Northeast US were cultured quarterly for MAP, providing 1131 samples (133 (11.8%) were culture-positive), and all adult animals in the herds were tested biannually by fecal culture (FC), for 6 years. Of the environmental sites sampled, manure storage areas and shared alleyways were most likely to be culture-positive. Environmental sample results were compared to FC results from either the concurrent or previous sampling date at both the herd and the pen level. At the herd level, a 1 log unit increase in average fecal shedding increased the odds of a positive non-pen environmental sample by a factor of 6 and increased the average amount of MAP in non-pen samples by 2.9 cfu/g. At the pen level, a 1 log unit increase in average fecal shedding in the pen increased the odds of a positive environment by a factor of 2.4 and the average amount of MAP was increased by 3.5 cfu/g. We were not able to model the relationship between non-pen environmental sample status and the distance between shedding animals and the sample's location, and neighboring pens did not significantly affect the results of the pen-level analysis. The amount of MAP in pen-level samples and the probability of a pen testing positive for MAP were both positively but non-significantly correlated with the number of animals in the pen shedding >30 cfu/g of MAP. At least 6 environmental samples met the criteria for the U.S. Voluntary Bovine Johne's Disease Control Program on 47 of the 72 sampling dates; of these, 19 of the 47 FC-positive sampling dates were positive by the 6-sample environmental testing method, resulting in a herd sensitivity of 0.40 (95% CI: 0.26-0.54). None of the 3 FC-negative sampling dates produced positive environmental samples. Although environmental sampling can be used as a tool in understanding the level of MAP infection in a herd or pen, it did not appear to be a sensitive diagnostic method for herd positivity in these low prevalence herds, and its use may require caution., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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24. Longitudinal study of a clonal, subclinical outbreak of Salmonella enterica subsp. enterica serovar Cerro in a U.S. dairy herd.
- Author
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Van Kessel JS, Karns JS, Wolfgang DR, Hovingh E, and Schukken YH
- Subjects
- Animals, Cattle, Colony Count, Microbial, Dairying methods, Feces microbiology, Female, Humans, Milk microbiology, Pennsylvania epidemiology, Serotyping, Cattle Diseases epidemiology, Disease Outbreaks veterinary, Phylogeny, Salmonella Infections, Animal epidemiology, Salmonella enterica classification
- Abstract
Salmonellae are a major group of foodborne pathogens known to affect both humans and animals. Dairy cattle are a known reservoir of these bacteria and human Salmonella infections have been associated with the consumption of improperly processed or contaminated dairy products. Many of the over 2500 known serotypes of Salmonella are known to infect cattle, resulting in asymptomatic to fatal salmonellosis. This study describes the course of a Salmonella outbreak and subsequent endemic infection on a dairy farm in Pennsylvania. The outbreak was initially detected when a few cows with clinical symptoms and one fatality were found to be infected with Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagan. Based upon sampling of the farm environment, Salmonella Typhimurium var. Copenhagan was succeeded within 3 months by Salmonella enterica subsp. enterica serovar Kentucky. Salmonella enterica subsp. enterica serovar Cerro ultimately supplanted Typhimurium var. Copenhagan and Kentucky in individual animals and environmental samples and persisted in the herd at high prevalence for almost 2 years. Since there were no obvious clinical consequences of the Salmonella Cerro infection, these data suggest that some serotypes of S. enterica subsp. enterica can behave as commensal organisms in dairy cattle and illustrate the difficulties of controlling Salmonella in milk production systems. The consistent finding of Salmonella in the environment reinforces the potential for human exposure to this pathogen and the need to understand the dynamics and ecology of Salmonella in dairy production settings.
- Published
- 2007
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25. Evaluation of IS900-PCR assay for detection of Mycobacterium avium subspecies Paratuberculosis infection in cattle using quarter milk and bulk tank milk samples.
- Author
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Jayarao BM, Pillai SR, Wolfgang DR, Griswold DR, Rossiter CA, Tewari D, Burns CM, and Hutchinson LJ
- Subjects
- Animals, Cattle, DNA, Bacterial analysis, Feces microbiology, Female, Food Contamination analysis, Lactation, Mass Screening veterinary, Polymerase Chain Reaction methods, Predictive Value of Tests, Sensitivity and Specificity, Cattle Diseases diagnosis, Milk microbiology, Mycobacterium avium subsp. paratuberculosis isolation & purification, Paratuberculosis diagnosis, Polymerase Chain Reaction veterinary
- Abstract
A study was conducted to evaluate sensitivity, specificity, and predictive value of IS900-PCR assay for detection of Mycobacterium avium subspecies paratuberculosis in pooled quarter milk and bulk tank milk. Feces, blood and pooled quarter milk from 1493 lactating cattle on 29 herds were analyzed. Bulk tank milk (n = 29 bulk tanks) samples were also examined. Culture analysis revealed that 10.9%, 2.8%, and 20.6% of fecal, pooled quarter milk samples and bulk tanks were positive for M. avium subsp. paratuberculosis, respectively. While 13.5% and 27.5% of pooled quarter milk samples and bulk tanks were positive by IS900 PCR assay, respectively. Moderate to high antibody titers for M. avium subsp. paratuberculosis were detected in 223 of 1493 (14.4%) cows. Cows positive on fecal culture were taken as true positives relative to which the IS900 PCR assay was evaluated. The sensitivity and predictive value of KELA, pooled quarter milk culture, and IS900 PCR assay increased with lactation age. While the specificity of the tests decreased with increase in lactation age. Overall, the IS900 PCR assay using pooled quarter milk samples had a sensitivity, specificity and predictive value of 0.87, 0.95 and 0.71, respectively. The IS900 PCR assay using bulk tank milk had poor sensitivity (0.21), specificity (0.5) and predictive value (0.6). Pooled quarter milk culture analysis had a very low sensitivity (0.17). The kinetics ELISA had lower sensitivity (0.59), specificity (0.90) and predictive value (0.43) as compared to the IS900 PCR assay using pooled quarter milk samples. Results from our study suggest that IS900 PCR assay using bulk tank milk may not be useful for screening herds with M. avium subsp. paratuberculosis infected animals. In conclusion, use of IS900 PCR assay for cows in 2(nd) lactation and higher, using aseptically collected pooled quarter milk samples, can be a useful tool for screening and monitoring lactating cattle in herds with M. avium subsp. paratuberculosis infection.
- Published
- 2004
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26. A computerized mastitis decision aid using farm-based records: an artificial neural network approach.
- Author
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Heald CW, Kim T, Sischo WM, Cooper JB, and Wolfgang DR
- Subjects
- Animals, Bacterial Infections microbiology, Bacterial Infections veterinary, Cattle, Cell Count, Computer Simulation, Discriminant Analysis, Female, Mastitis, Bovine microbiology, Milk cytology, Models, Statistical, Dairying methods, Mastitis, Bovine diagnosis, Neural Networks, Computer
- Abstract
A computer module was developed and tested that used field survey and Dairy Herd Improvement Association (DHIA) data to broadly classify bacterial causes of mastitis in dairy herds. Further development of the computer model could aid interpretation of DHIA data by dairy record processing centers and herd consultants. This diagnostic module was developed with an artificial neural network, a technology that processes complex data in a manner similar to human brain function. Information describing herd management practices, quarter milk samples, and monthly DHIA data was collected from Pennsylvania dairy herds with moderate to high somatic cell counts. This information was used to develop or train an artificial neural network model that discriminated among four categories of bacterial organisms (contagious, environmental, no significant growth, and other) associated with clinical and subclinical mastitis. After training the model, new DHIA and management data were presented to the model to assess its ability to classify bacteriological etiology. When the artificial neural network was used, the probabilities of diagnosing the bacteriologic status from three randomly selected cow groups and from new untested herds ranged from 57 to 71%. Performance of the artificial neural network model was best in herds with higher frequency of minor and contagious pathogens. Prediction results for the same test data with linear discriminant analysis were less successful, ranging from 42 to 57%.
- Published
- 2000
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27. Characteristics of veal calves upon arrival, at 28 and 84 days, and at end of the production cycle.
- Author
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Wilson LL, Smith JL, Smith DL, Swanson DL, Drake TR, Wolfgang DR, and Wheeler EF
- Subjects
- Aging, Animal Nutritional Physiological Phenomena, Animals, Blood Proteins analysis, Body Weight, Cattle growth & development, Erythrocyte Count, Health Status, Hematocrit, Hemoglobins analysis, Leukocyte Count, Male, Seasons, Cattle blood, Cattle physiology, Weight Gain
- Abstract
Holstein bull calves arriving at five special-fed veal farms (eight production groups) were scored for various physical condition traits and blood-sampled within 2 h after arrival and at 28 d, 84 d, and 1 wk prior to slaughter at 116 to 143 d. Of 1179 total calves in the production groups, 758 calves were scored and evaluated. Blood cell analyses (red and white blood cell counts, hemoglobin, and packed cell volume) were conducted at all four sampling times; total serum protein concentration was obtained at 0 and 28 d. The study was initiated in autumn and ended the following autumn. Mean initial and final body weights were 46.3+/-0.17 and 209.7+/-0.77 kg; mean mortality was 2.5%. Average daily gain of the eight groups ranged from 1.23 to 1.70 kg/d. Subjective scores of 5 = excellent to 1 = very poor condition were used to evaluate 16 different physical condition characteristics. With the exception of leg joint, hoof, and foot scores, most of the physical condition scores exhibited improvement during the first 28 d. Foot and leg impairments did not appear to hinder the ambulatory ability of the calves during the production period. Physical condition scores at d 0 and 28 were generally not related to numbers or types of medical treatments (enteric, respiratory, other, or total) or to average daily gain during the production period. Means for most erythrocytic and leukocytic traits upon arrival (d 0) were within normal ranges, although 27.4% of the calves were clinically or marginally anemic. Final mean hemoglobin and packed cell volume were 8.53 g/dl and 26.1%. Forty-three percent of the calves at d 0 were colostral deficient, assuming that total serum protein concentrations of <5.5 g/dl indicate colostral deficiency. No blood trait was consistently correlated with body weight gain when gain during the production period was divided into quartiles and the blood traits were averaged by gain quartile. Calves in the lowest serum total protein quartile (mean 4.58 g/dl) had more respiratory and total medical treatments than quartiles with higher total protein means. Dairy bull calves arriving at veal production units after transporting from the dairy farm to the auction market (or other collection facility) have several physical impairments. However, most of these physical impairments are improved early in the veal feeding period and are not generally related to subsequent growth rate or medical treatment.
- Published
- 2000
- Full Text
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