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Your search keyword '"Wooding, Carol"' showing total 20 results
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20 results on '"Wooding, Carol"'

7. Taxation.

Author

Berry, Jeffrey D. and Wooding, Carol A.

Subjects
State taxation -- Surveys, Local taxation -- Surveys
Published
1990

8. KDM2A integrates DNA and histone modification signals through a CXXC/PHD module and direct interaction with HP1

Author

Borgel, Julie, Tyl, Marek, Schiller, Karin, Pusztai, Zsofia, Dooley, Christopher M., Deng, Wen, Wooding, Carol, White, Richard J., Warnecke, Tobias, Leonhardt, Heinrich, Busch-Nentwich, Elisabeth M., Bartke, Till, Busch-Nentwich, Elisabeth [0000-0001-6450-744X], Apollo - University of Cambridge Repository, and Commission of the European Communities

Subjects
Biochemistry & Molecular Biology, endocrine system, Jumonji Domain-Containing Histone Demethylases, animal structures, PROTEINS, Chromosomal Proteins, Non-Histone, Recombinant Fusion Proteins, 05 Environmental Sciences, CANCER GENOMICS, SATELLITE REPEATS, Cell Line, Animals, Genetically Modified, Histones, DOMAIN, Cricetinae, Neoplasms, Two-Hybrid System Techniques, Animals, Humans, Protein Interaction Domains and Motifs, TRANSCRIPTION, Zebrafish, 08 Information And Computing Sciences, Science & Technology, Models, Genetic, F-Box Proteins, Gene regulation, Chromatin and Epigenetics, MAMMALIAN HETEROCHROMATIN, RECOGNITION, METHYLATION, Zinc Fingers, 06 Biological Sciences, DNA Methylation, Nucleosomes, HEK293 Cells, Chromobox Protein Homolog 5, CELLS, embryonic structures, Mutation, DEMETHYLASE KDM2A, CpG Islands, Life Sciences & Biomedicine, Developmental Biology, HeLa Cells
Abstract

Functional genomic elements are marked by characteristic DNA and histone modification signatures. How combinatorial chromatin modification states are recognized by epigenetic reader proteins and how this is linked to their biological function is largely unknown. Here we provide a detailed molecular analysis of chromatin recognition by the lysine demethylase KDM2A. Using biochemical approaches we identify a nucleosome interaction module within KDM2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding between KDM2A and HP1. This nucleosome interaction module enables KDM2A to decode nucleosomal H3K9me3 modification in addition to CpG methylation signals. The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM2A can be recruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A. A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression assay in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function. Our results reveal a complex regulation of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and suggest a direct role for KDM2A in chromatin silencing.

Published
2017

9. KDM2A integrates DNA and histone modification signals through a CXXC/PHD module and direct interaction with HP1

Author

Borgel, Julie, primary, Tyl, Marek, additional, Schiller, Karin, additional, Pusztai, Zsofia, additional, Dooley, Christopher M., additional, Deng, Wen, additional, Wooding, Carol, additional, White, Richard J., additional, Warnecke, Tobias, additional, Leonhardt, Heinrich, additional, Busch-Nentwich, Elisabeth M., additional, and Bartke, Till, additional

Published
2016
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14. Renal Chloride Channel, CLCN5, Mutations in Dent's Disease

Author

Cox, Jeremy P. D., primary, Yamamoto, Katsusuke, additional, Christie, Paul T., additional, Wooding, Carol, additional, Feest, Terry, additional, Flinter, Frances A., additional, Goodyer, Paul R., additional, Leumann, Ernst, additional, Neuhaus, Thomas, additional, Reid, Christopher, additional, Williams, Paul F., additional, Wrong, Oliver, additional, and Thakker, Rajesh V., additional

Published
1999
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15. Mutational Analysis of PHEX Gene in X-Linked Hypophosphatemia1

Author

Dixon, Peter H., primary, Christie, Paul T., additional, Wooding, Carol, additional, Trump, Dorothy, additional, Grieff, Marvin, additional, Holm, Ingrid, additional, Gertner, Joseph M., additional, Schmidtke, Jorg, additional, Shah, Binita, additional, Shaw, Nicholas, additional, Smith, Colin, additional, Tau, Christina, additional, Schlessinger, David, additional, Whyte, Michael P., additional, and Thakker, Rajesh V., additional

Published
1998
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17. Genetic mapping studies of 40 loci and 23 cosmids in chromosome 11p13-11q13, and exclusion of Ì-calpain as the multiple endocrine neoplasia type 1 gene

Author

Pang, Joanna T., primary, Lloyd, Sarah E., additional, Wooding, Carol, additional, Farren, Bernadette, additional, Pottinger, Bruce, additional, Harding, Brian, additional, Leigh, Sarah E. A., additional, Pook, Mark A., additional, Benham, Frances J., additional, Gillett, Godfrey T., additional, Taggart, R. Thomas, additional, and Thakker, R. V., additional

Published
1996
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20. KDM2A integrates DNA and histone modification signals through a CXXC/PHD module and direct interaction with HP1.

Author

Borgel J, Tyl M, Schiller K, Pusztai Z, Dooley CM, Deng W, Wooding C, White RJ, Warnecke T, Leonhardt H, Busch-Nentwich EM, and Bartke T

Subjects
Animals, Animals, Genetically Modified, Cell Line, Chromobox Protein Homolog 5, Chromosomal Proteins, Non-Histone genetics, CpG Islands, Cricetinae, DNA Methylation, F-Box Proteins genetics, HEK293 Cells, HeLa Cells, Histones metabolism, Humans, Jumonji Domain-Containing Histone Demethylases genetics, Models, Genetic, Mutation, Neoplasms genetics, Neoplasms metabolism, Nucleosomes genetics, Nucleosomes metabolism, Protein Interaction Domains and Motifs, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Two-Hybrid System Techniques, Zebrafish, Zinc Fingers, Chromosomal Proteins, Non-Histone chemistry, Chromosomal Proteins, Non-Histone metabolism, F-Box Proteins chemistry, F-Box Proteins metabolism, Jumonji Domain-Containing Histone Demethylases chemistry, Jumonji Domain-Containing Histone Demethylases metabolism
Abstract

Functional genomic elements are marked by characteristic DNA and histone modification signatures. How combinatorial chromatin modification states are recognized by epigenetic reader proteins and how this is linked to their biological function is largely unknown. Here we provide a detailed molecular analysis of chromatin recognition by the lysine demethylase KDM2A. Using biochemical approaches we identify a nucleosome interaction module within KDM2A consisting of a CXXC type zinc finger, a PHD domain and a newly identified Heterochromatin Protein 1 (HP1) interaction motif that mediates direct binding between KDM2A and HP1. This nucleosome interaction module enables KDM2A to decode nucleosomal H3K9me3 modification in addition to CpG methylation signals. The multivalent engagement with DNA and HP1 results in a nucleosome binding circuit in which KDM2A can be recruited to H3K9me3-modified chromatin through HP1, and HP1 can be recruited to unmodified chromatin by KDM2A. A KDM2A mutant deficient in HP1-binding is inactive in an in vivo overexpression assay in zebrafish embryos demonstrating that the HP1 interaction is essential for KDM2A function. Our results reveal a complex regulation of chromatin binding for both KDM2A and HP1 that is modulated by DNA- and H3K9-methylation, and suggest a direct role for KDM2A in chromatin silencing.

Published
2017
Full Text
View/download PDF

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