1. Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca 2+ /ERK pathway-enhanced aberrant osteogenic activity.
- Author
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Yuan Z, Li J, He K, Sun Z, Luo G, Liu H, Dong J, Zhou C, Cui H, and Fan C
- Subjects
- Animals, Calcium metabolism, Male, Stem Cells metabolism, Stem Cells cytology, Cell Differentiation drug effects, Zinc Oxide chemistry, Zinc Oxide pharmacology, Rats, Tendons metabolism, Tendons pathology, Humans, Tendon Injuries metabolism, Tendon Injuries pathology, Nanoparticles chemistry, Wounds and Injuries metabolism, Wounds and Injuries pathology, Wounds and Injuries complications, Hydrogen Sulfide metabolism, Hydrogen Sulfide pharmacology, Ossification, Heterotopic metabolism, Ossification, Heterotopic pathology, Osteogenesis drug effects, MAP Kinase Signaling System drug effects
- Abstract
Unveiling of the mechanism involved in the occurrence and development of trauma-induced heterotopic ossification (tHO) is highly demanding due to current ineffective clinical treatment for it. Previous studies proposed that hydrogen sulfide (H
2 S) was vital for fate determination of stem cells, suggesting a potential role in the regulation of tHO development. In the current study, We found that expression of metabolic enzyme within sulfur conversion pathway was enhanced after tendon injury, leading to H2 S accumulation within the tHO region. Increased production of endogenous H2 S was shown to promote aberrant osteogenic activity of tendon-derived stem cells (TDSCs), which accelerated tHO formation. The inhibition of metabolic enzyme of H2 S production or directly absorption of H2 S could abolished osteogenic induction of TDSCs and the formation of tHO. Mechanistically, through RNA sequencing combined with rescue experiments, we demonstrated that activation of Ca2+ /ERK pathway was the downstream molecular event of H2 S-induced osteogenic commitment of TDSCs and tHO. For treatment strategy exploration, zine oxide nanoparticles (ZnO) as an effective H2 S elimination material was validated to ideally halt the tHO formation in this study. Furthermore, in terms of chirality of nanoparticles, D-ZnO or L-ZnO nanoparticles showed superiority over R-ZnO nanoparticles in both clearing of H2 S and inhibition of tHO. Our study not only revealed the mechanism of tHO through the endogenous gas signaling event from a new perspective, but also presented a applicable platform for elimination of the inordinate gas production, thus aiding the development of clinical treatment for tHO., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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