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Bacteria belonging to the genus Micromonospora are recognized as microorganisms with the potential to be used in biotechnology processes, given their beneficial influence on plant growth and the biocontrol of phytopathogens. In this study, nineteen Micromonospora isolates originating from the root nodules of white clover plants were taxonomically assigned based on the phylogenetic analysis of the 16S rRNA gene and four housekeeping genes. The antifungal properties of the bacteria against phytopathogenic Botrytis cinerea, Fusarium oxysporum, Fusarium equiseti, Sclerotinia sclerotiorum, and Verticillium albo-atrum were tested with the agar plug test and the dual culture test. The ability to produce various metallophores was determined with the agar plug diffusion test on modified chrome azurol S (CAS) agar medium. International Streptomyces Project-2 medium (ISP2) broth amended with 0.2% L-tryptophan was used to indicate the bacterial ability to produce auxins. The strains belonging to M. tulbaghiae, M. inaquosa, and M. violae showed in vitro potential as antimicrobial agents against the tested fungi. M. inaquosa strain 152, M. violae strain 126, M. violae strain 66, and M. violae strain 45 were recognized as the most efficient metallophore producers. M. alfalfae strain 55 and M. lupini strain 5052 were identified as the most promising auxin compound producers and, therefore, show potential as plant-growth-promoting bacteria. [ABSTRACT FROM AUTHOR]

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Purpose: Allergic diseases have escalated to epidemic levels worldwide, impacting nearly 30% of the global population. Fungi are a significant source of allergens responsible for up to 6% of respiratory diseases in the general population. However, the specific cause of respiratory allergies often remains unidentified. This study aimed to investigate the potential of two common rust fungi, Tranzschelia pruni-spinosae and Phragmidium rubi-idaei , to trigger a proinflammatory response in vitro models representing the upper and lower respiratory tract., Materials and Methods: The BEAS-2B and A549 cell lines simulated upper and lower respiratory endothelial cells. The cytotoxicity of fungal extracts was evaluated using MTT and flow cytometry assays. Cell reactive oxygen species (ROS) production was measured via flow cytometry, while ELISA tests quantified the production of proinflammatory cytokines. Immunofluorescence techniques were employed to assess cell integrity markers., Results: Extracts from T. pruni-spinosae and P. rubi-idaei significantly stimulated the production of proinflammatory cytokines IL-1β and GM-CSF in both cell lines, all of which are associated with the development of allergic responses. The increase in these cytokines and the elevated ROS production were linked to the disruption of epithelial cell junctions., Conclusion: The findings suggest the potential of T. pruni-spinosae and P. rubi-idaei extracts to collectively disrupt the epithelial barrier in the upper and lower respiratory tract by inducing proinflammatory cytokines and the production of reactive oxygen species and metalloproteinases. Although none of the above parameters was spectacularly high, all of them together could cause a decrease in the presence of tight junction proteins, such as E-cadherin and occludin, in epithelial cells., Competing Interests: The authors declare that the research was conducted without any commercial or financial relationship that could be construed as a potential conflict of interest., (© 2025 Sztandera-Tymoczek et al.)