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1. Severe disease during both primary and secondary dengue virus infections in pediatric populations

Author

Aggarwal, Charu, Ahmed, Hasan, Sharma, Pragati, Reddy, Elluri Seetharami, Nayak, Kaustuv, Singla, Mohit, Maheshwari, Deepti, Chawla, Yadya M., Panda, Harekrushna, Rai, Ramesh Chandra, Gunisetty, Sivaram, Priyamvada, Lalita, Bhaumik, Siddhartha Kumar, Ahamed, Syed Fazil, Vivek, Rosario, Bhatnagar, Priya, Singh, Prabhat, Kaur, Manpreet, Dixit, Kritika, Kumar, Sanjeev, Gottimukkala, Kamal, Saini, Keshav, Bajpai, Prashant, Sreekanth, Gopinathan Pillai, Mammen, Shobha, Rajan, Anand, Verghese, Valsan Philip, Abraham, Asha Mary, Shah, Paresh, Alagarasu, Kalichamy, Yu, Tianwei, Davis, Carl W., Wrammert, Jens, Ansari, Aftab, Antia, Rustom, Kabra, Sushil Kumar, Medigeshi, Guruprasad R., Ahmed, Rafi, Lodha, Rakesh, Shet, Anita, Chandele, Anmol, and Murali-Krishna, Kaja

Published
2024
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2. Epistasis reduces fitness costs of influenza A virus escape from stem-binding antibodies

Author

Lee, Chung-Young, Raghunathan, Vedhika, Caceres, C Joaquin, Geiger, Ginger, Seibert, Brittany, Faccin, Flavio Cargnin, Gay, L Claire, Ferreri, Lucas M, Kaul, Drishti, Wrammert, Jens, Tan, Gene S, Perez, Daniel R, and Lowen, Anice C

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Biological Sciences, Biodefense, Genetics, Vaccine Related, Infectious Diseases, Pneumonia & Influenza, Emerging Infectious Diseases, Prevention, Biotechnology, Influenza, Immunization, Stem Cell Research, Infection, Good Health and Well Being, Humans, Influenza A virus, Antibodies, Neutralizing, Antibodies, Viral, Broadly Neutralizing Antibodies, Epistasis, Genetic, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines, Hemagglutinins, Influenza, Human, influenza A virus, HA stem, evolution, antigenic escape, epistasis
Abstract

The hemagglutinin (HA) stem region is a major target of universal influenza vaccine efforts owing to the presence of highly conserved epitopes across multiple influenza A virus (IAV) strains and subtypes. To explore the potential impact of vaccine-induced immunity targeting the HA stem, we examined the fitness effects of viral escape from stem-binding broadly neutralizing antibodies (stem-bnAbs). Recombinant viruses containing each individual antibody escape substitution showed diminished replication compared to wild-type virus, indicating that stem-bnAb escape incurred fitness costs. A second-site mutation in the HA head domain (N129D; H1 numbering) reduced the fitness effects observed in primary cell cultures and likely enabled the selection of escape mutations. Functionally, this putative permissive mutation increased HA avidity for its receptor. These results suggest a mechanism of epistasis in IAV, wherein modulating the efficiency of attachment eases evolutionary constraints imposed by the requirement for membrane fusion. Taken together, the data indicate that viral escape from stem-bnAbs is costly but highlights the potential for epistatic interactions to enable evolution within the functionally constrained HA stem domain.

Published
2023

5. Multi-omics analysis of mucosal and systemic immunity to SARS-CoV-2 after birth

Author

Wimmers, Florian, Burrell, Allison R., Feng, Yupeng, Zheng, Hong, Arunachalam, Prabhu S., Hu, Mengyun, Spranger, Sara, Nyhoff, Lindsay E., Joshi, Devyani, Trisal, Meera, Awasthi, Mayanka, Bellusci, Lorenza, Ashraf, Usama, Kowli, Sangeeta, Konvinse, Katherine C., Yang, Emily, Blanco, Michael, Pellegrini, Kathryn, Tharp, Gregory, Hagan, Thomas, Chinthrajah, R. Sharon, Nguyen, Tran T., Grifoni, Alba, Sette, Alessandro, Nadeau, Kari C., Haslam, David B., Bosinger, Steven E., Wrammert, Jens, Maecker, Holden T., Utz, Paul J., Wang, Taia T., Khurana, Surender, Khatri, Purvesh, Staat, Mary A., and Pulendran, Bali

Published
2023
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6. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response

Author

Patel, Anamika, Kumar, Sanjeev, Lai, Lilin, Chakravarthy, Chennareddy, Valanparambil, Rajesh, Reddy, Elluri Seetharami, Gottimukkala, Kamalvishnu, Bajpai, Prashant, Raju, Dinesh Ravindra, Edara, Venkata Viswanadh, Davis-Gardner, Meredith E., Linderman, Susanne, Dixit, Kritika, Sharma, Pragati, Mantus, Grace, Cheedarla, Narayanaiah, Verkerke, Hans P., Frank, Filipp, Neish, Andrew S., Roback, John D., Davis, Carl W., Wrammert, Jens, Ahmed, Rafi, Suthar, Mehul S., Sharma, Amit, Murali-Krishna, Kaja, Chandele, Anmol, and Ortlund, Eric A.

Published
2023
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7. Altered amino acid profile in patients with SARS-CoV-2 infection

Author

Rees, Chris A., Rostad, Christina A., Mantus, Grace, Anderson, Evan J., Chahroudi, Ann, Jaggi, Preeti, Wrammert, Jens, Ochoa, Juan B., Ochoa, Augusto, Basu, Rajit K., Heilman, Stacy, Harris, Frank, Lapp, Stacey A., Hussaini, Laila, Vos, Miriam B., Brown, Lou Ann, and Morris, Claudia R.

Published
2021

10. Incidence of SARS-CoV-2 Seropositivity in Pediatric Healthcare Workers Prior to Widespread Vaccination: A Five-month Longitudinal Cohort Study

Author

Griffiths, Mark, primary, Hatabah, Dunia, additional, Sullivan, Patrick, additional, Mantus, Grace, additional, Sanchez, Travis, additional, Zlotorzynska, Maria, additional, Heilman, Stacy, additional, Camacho-Gonzalez, Andres, additional, Leake, Deborah, additional, Korman, Rawan, additional, Le, Mimi, additional, Suthara, Mehul, additional, Wrammert, Jens, additional, Vos, Miriam B., additional, and Morris, Claudia R., additional

Published
2024
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11. Endemic Coronavirus Infections are Associated with Strong Homotypic Immunity in a US Cohort of Children from Birth to 4 Years

Author

Morrow, Ardythe L, primary, Payne, Daniel C, additional, Conrey, Shannon C, additional, McMorrow, Meredith, additional, McNeal, Monica M, additional, Niu, Liang, additional, Burrell, Allison R, additional, Schlaudecker, Elizabeth P, additional, Mattison, Claire, additional, Burke, Rachel M, additional, DeFranco, Emily, additional, Teoh, Zheyi, additional, Wrammert, Jens, additional, Atherton, Lydia J, additional, Thornburg, Natalie J, additional, and Staat, Mary A, additional

Published
2024
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12. XBB.1.5 monovalent booster improves antibody binding and neutralization against emerging SARS-CoV-2 Omicron variants

Author

Jain, Shilpi, primary, Kumar, Sanjeev, additional, Lai, Lilin, additional, Linderman, Susanne, additional, Malik, Ansa A., additional, Ellis, Madison L., additional, Godbole, Sucheta, additional, Solis, Daniel, additional, Sahoo, Malaya K., additional, Bechnak, Kareem, additional, Paredes, Isabel, additional, Tanios, Ralph, additional, Kazzi, Bahaa, additional, Dib, Serena M., additional, Litvack, Matthew B, additional, Wimalasena, Sonia T., additional, Ciric, Caroline, additional, Rostad, Christina, additional, West, Richard, additional, Teng, I-Ting, additional, Wang, Danyi, additional, Edupuganti, Sri, additional, Kwong, Peter D., additional, Rouphael, Nadine, additional, Pinsky, Benjamin A., additional, Douek, Daniel C., additional, Wrammert, Jens, additional, Moreno, Alberto, additional, and Suthar, Mehul S., additional

Published
2024
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13. Longitudinal analysis shows durable and broad immune memory after SARS-CoV-2 infection with persisting antibody responses and memory B and T cells

Author

Cohen, Kristen W., Linderman, Susanne L., Moodie, Zoe, Czartoski, Julie, Lai, Lilin, Mantus, Grace, Norwood, Carson, Nyhoff, Lindsay E., Edara, Venkata Viswanadh, Floyd, Katharine, De Rosa, Stephen C., Ahmed, Hasan, Whaley, Rachael, Patel, Shivan N., Prigmore, Brittany, Lemos, Maria P., Davis, Carl W., Furth, Sarah, O’Keefe, James B., Gharpure, Mohini P., Gunisetty, Sivaram, Stephens, Kathy, Antia, Rustom, Zarnitsyna, Veronika I., Stephens, David S., Edupuganti, Srilatha, Rouphael, Nadine, Anderson, Evan J., Mehta, Aneesh K., Wrammert, Jens, Suthar, Mehul S., Ahmed, Rafi, and McElrath, M. Juliana

Published
2021
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14. Prevalence of SARS-CoV-2 antibodies in pediatric healthcare workers

Author

Morris, Claudia R., Sullivan, Patrick, Mantus, Grace, Sanchez, Travis, Zlotorzynska, Maria, Hanberry, Bradley, Iyer, Srikant, Heilman, Stacy, Camacho-Gonzalez, Andres, Figueroa, Janet, Manoranjithan, Shaminy, Leake, Deborah, Mendis, Reshika, Cleeton, Rebecca, Chen, Christie, Krieger, Rachel, Bush, Patricia, Hughes, Tiffany, Little, Wendalyn K., Suthar, Mehul S., Wrammert, Jens, and Vos, Miriam B.

Published
2021
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15. Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells

Author

Kauffman, Robert C, Bhuiyan, Taufiqur R, Nakajima, Rie, Mayo-Smith, Leslie M, Rashu, Rasheduzzaman, Hoq, Mohammad Rubel, Chowdhury, Fahima, Khan, Ashraful Islam, Rahman, Atiqur, Bhaumik, Siddhartha K, Harris, Levelle, O'Neal, Justin T, Trost, Jessica F, Alam, Nur Haq, Jasinskas, Algis, Dotsey, Emmanuel, Kelly, Meagan, Charles, Richelle C, Xu, Peng, Kováč, Pavol, Calderwood, Stephen B, Ryan, Edward T, Felgner, Phillip L, Qadri, Firdausi, Wrammert, Jens, Harris, Jason B, Petri, William, Kaper, James, and Sack, David

Subjects
Biodefense, Clinical Research, Vaccine Related, Foodborne Illness, Prevention, Biotechnology, Immunization, Emerging Infectious Diseases, Infectious Diseases, Digestive Diseases, Infection, Good Health and Well Being, Adult, Antibodies, Bacterial, Antibodies, Monoclonal, Antibody Formation, B-Lymphocytes, Bacterial Toxins, Cholera, Cholera Toxin, Cross Reactions, Enterotoxins, Escherichia coli Proteins, Humans, Immunologic Memory, Lipopolysaccharides, Neuraminidase, O Antigens, Proteomics, Serogroup, Single-Cell Analysis, Vibrio cholerae, Microbiology
Abstract

We characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels of somatic hypermutation and large clonal expansions. A majority of the expansions targeted cholera toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we were able to identify sialidase as another major antigen targeted by the antibody response to Vibrio cholerae infection. Antitoxin MAbs targeted both the A and B subunits, and most were also potent neutralizers of enterotoxigenic Escherichia coli heat-labile toxin. LPS-specific MAbs uniformly targeted the O-specific polysaccharide, with no detectable responses to either the core or the lipid moiety of LPS. Interestingly, the LPS-specific antibodies varied widely in serotype specificity and functional characteristics. One participant infected with the Ogawa serotype produced highly mutated LPS-specific antibodies that preferentially bound the previously circulating Inaba serotype. This demonstrates durable memory against a polysaccharide antigen presented at the mucosal surface and provides a mechanism for the long-term, partial heterotypic immunity seen following cholera.ImportanceCholera is a diarrheal disease that results in significant mortality. While oral cholera vaccines are beneficial, they do not achieve equivalent protection compared to infection with Vibrio cholerae Although antibodies likely mediate protection, the mechanisms of immunity following cholera are poorly understood, and a detailed understanding of antibody responses to cholera is of significance for human health. In this study, we characterized the human response to cholera at the single-plasmablast, monoclonal antibody level. Although this approach has not been widely applied to the study of human bacterial infection, we were able to uncover the basis of cross-reactivity between different V. cholerae serotypes and the likely impact of prior enterotoxigenic Escherichia coli exposure on the response to cholera, as well as identify novel antigenic targets. In addition to improving our understanding of the repertoire and function of the antibody response to cholera in humans, this study has implications for future cholera vaccination efforts.

Published
2016

16. Rapid Generation of Neutralizing Antibody Responses in COVID-19 Patients

Author

Suthar, Mehul S., Zimmerman, Matthew G., Kauffman, Robert C., Mantus, Grace, Linderman, Susanne L., Hudson, William H., Vanderheiden, Abigail, Nyhoff, Lindsay, Davis, Carl W., Adekunle, Oluwaseyi, Affer, Maurizio, Sherman, Melanie, Reynolds, Stacian, Verkerke, Hans P., Alter, David N., Guarner, Jeannette, Bryksin, Janetta, Horwath, Michael C., Arthur, Connie M., Saakadze, Natia, Smith, Geoffrey H., Edupuganti, Srilatha, Scherer, Erin M., Hellmeister, Kieffer, Cheng, Andrew, Morales, Juliet A., Neish, Andrew S., Stowell, Sean R., Frank, Filipp, Ortlund, Eric, Anderson, Evan J., Menachery, Vineet D., Rouphael, Nadine, Mehta, Aneesh K., Stephens, David S., Ahmed, Rafi, Roback, John D., and Wrammert, Jens

Published
2020
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17. 1103. Endemic Coronavirus Infections in a US Cohort of Children from Birth to 4 Years

Author

Payne, Daniel C, primary, Morrow, Ardythe L, additional, Conrey, Shannon C, additional, McMorrow, Meredith L, additional, McNeal, Monica, additional, Niu, Liang, additional, Burrell, Allison, additional, Schlaudecker, Elizabeth P, additional, Mattison, Claire, additional, Burke, Rachel M, additional, DeFranco, Emily A, additional, Teoh, Zheyi, additional, Wrammert, Jens, additional, Atherton, Lydia J, additional, Thornburg, Natalie J, additional, and Staat, Mary A, additional

Published
2023
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20. Light chain of a public SARS-CoV-2 class-3 antibody modulates neutralization against Omicron

Author

Patel, Anamika, primary, Kumar, Sanjeev, additional, Lai, Lilin, additional, Keen, Meredith, additional, Valanparambil, Rajesh, additional, Chakravarthy, Chennareddy, additional, Laughlin, Zane, additional, Frank, Filipp, additional, Cheedarla, Narayanaiah, additional, Verkerke, Hans P., additional, Neish, Andrew S., additional, Roback, John D., additional, Davis, Carl W., additional, Wrammert, Jens, additional, Sharma, Amit, additional, Ahmed, Rafi, additional, Suthar, Mehul S., additional, Murali-Krishna, Kaja, additional, Chandele, Anmol, additional, and Ortlund, Eric, additional

Published
2023
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27. B Cell Responses to Influenza Infection and Vaccination

Author

Chiu, Christopher, Ellebedy, Ali H., Wrammert, Jens, Ahmed, Rafi, Compans, Richard W, Series editor, Cooper, Max D., Series editor, Gleba, Yuri Y., Series editor, Honjo, Tasuku, Series editor, Oldstone, Michael B. A., Series editor, Vogt, Peter K., Series editor, Malissen, Bernard, Series editor, Aktories, Klaus, Series editor, Kawaoka, Yoshihiro, Series editor, Rappuoli, Rino, Series editor, Galan, Jorge E., Series editor, Ahmed, Rafi, Series editor, and Compans, Richard W., editor

Published
2015
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28. Infants and young children generate more durable antibody responses to SARS-CoV-2 infection than adults

Author

Joshi, Devyani, primary, Nyhoff, Lindsay E, additional, Zarnitsyna, Veronika I, additional, Moreno, Alberto, additional, Manning, Kelly, additional, Linderman, Susanne, additional, Burrell, Allison, additional, Stephens, Kathy, additional, Norwood, Carson, additional, Mantus, Grace E, additional, Ahmed, Rafi, additional, Anderson, Evan J, additional, Staat, Mary A, additional, Suthar, Mehul S, additional, and Wrammert, Jens, additional

Published
2023
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29. Supplementary Figure SF1 from Antibody Binding and Neutralization of Live SARS-CoV-2 Variants Including BA.4/5 Following Booster Vaccination of Patients with B-cell Malignancies

Author

Chang, Andres, primary, Akhtar, Akil, primary, Lai, Lilin, primary, Orellana-Noia, Victor M., primary, Linderman, Susanne L., primary, McCook-Veal, Ashley A., primary, Switchenko, Jeffrey M., primary, Saini, Manpreet, primary, Valanparambil, Rajesh M., primary, Blum, Kristie A., primary, Allen, Pamela B., primary, Lechowicz, Mary Jo, primary, Romancik, Jason T., primary, Ayers, Amy, primary, Leal, Alyssa, primary, O'Leary, Colin B., primary, Churnetski, Michael C., primary, Baird, Katelin, primary, Kives, Melissa, primary, Wrammert, Jens, primary, Nooka, Ajay K., primary, Koff, Jean L., primary, Dhodapkar, Madhav V., primary, Suthar, Mehul S., primary, Cohen, Jonathon B., primary, and Ahmed, Rafi, primary

Published
2023
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30. Supplementary Table ST3 from Antibody Binding and Neutralization of Live SARS-CoV-2 Variants Including BA.4/5 Following Booster Vaccination of Patients with B-cell Malignancies

Author

Chang, Andres, primary, Akhtar, Akil, primary, Lai, Lilin, primary, Orellana-Noia, Victor M., primary, Linderman, Susanne L., primary, McCook-Veal, Ashley A., primary, Switchenko, Jeffrey M., primary, Saini, Manpreet, primary, Valanparambil, Rajesh M., primary, Blum, Kristie A., primary, Allen, Pamela B., primary, Lechowicz, Mary Jo, primary, Romancik, Jason T., primary, Ayers, Amy, primary, Leal, Alyssa, primary, O'Leary, Colin B., primary, Churnetski, Michael C., primary, Baird, Katelin, primary, Kives, Melissa, primary, Wrammert, Jens, primary, Nooka, Ajay K., primary, Koff, Jean L., primary, Dhodapkar, Madhav V., primary, Suthar, Mehul S., primary, Cohen, Jonathon B., primary, and Ahmed, Rafi, primary

Published
2023
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31. Data from Antibody Binding and Neutralization of Live SARS-CoV-2 Variants Including BA.4/5 Following Booster Vaccination of Patients with B-cell Malignancies

Author

Chang, Andres, primary, Akhtar, Akil, primary, Lai, Lilin, primary, Orellana-Noia, Victor M., primary, Linderman, Susanne L., primary, McCook-Veal, Ashley A., primary, Switchenko, Jeffrey M., primary, Saini, Manpreet, primary, Valanparambil, Rajesh M., primary, Blum, Kristie A., primary, Allen, Pamela B., primary, Lechowicz, Mary Jo, primary, Romancik, Jason T., primary, Ayers, Amy, primary, Leal, Alyssa, primary, O'Leary, Colin B., primary, Churnetski, Michael C., primary, Baird, Katelin, primary, Kives, Melissa, primary, Wrammert, Jens, primary, Nooka, Ajay K., primary, Koff, Jean L., primary, Dhodapkar, Madhav V., primary, Suthar, Mehul S., primary, Cohen, Jonathon B., primary, and Ahmed, Rafi, primary

Published
2023
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32. An inactivated NDV-HXP-S COVID-19 vaccine elicits a higher proportion of neutralizing antibodies in humans than mRNA vaccination

Author

Carreño, Juan Manuel, primary, Raskin, Ariel, additional, Singh, Gagandeep, additional, Tcheou, Johnstone, additional, Kawabata, Hisaaki, additional, Gleason, Charles, additional, Srivastava, Komal, additional, Vigdorovich, Vladimir, additional, Dambrauskas, Nicholas, additional, Gupta, Sneh Lata, additional, González Domínguez, Irene, additional, Martinez, Jose Luis, additional, Slamanig, Stefan, additional, Sather, D. Noah, additional, Raghunandan, Rama, additional, Wirachwong, Ponthip, additional, Muangnoicharoen, Sant, additional, Pitisuttithum, Punnee, additional, Wrammert, Jens, additional, Suthar, Mehul S., additional, Sun, Weina, additional, Palese, Peter, additional, García-Sastre, Adolfo, additional, Simon, Viviana, additional, and Krammer, Florian, additional

Published
2023
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34. Systems biological assessment of the temporal dynamics of immunity to a viral infection in the first weeks and months of life

Author

Wimmers, Florian, primary, Burrell, Allison R., additional, Feng, Yupeng, additional, Zheng, Hong, additional, Arunachalam, Prabhu S., additional, Hu, Mengyun, additional, Spranger, Sara, additional, Nyhoff, Lindsay, additional, Joshi, Devyani, additional, Trisal, Meera, additional, Awasthi, Mayanka, additional, Bellusci, Lorenza, additional, Ashraf, Usama, additional, Kowli, Sangeeta, additional, Konvinse, Katherine C., additional, Yang, Emily, additional, Blanco, Michael, additional, Pellegrini, Kathryn, additional, Tharp, Gregory, additional, Hagan, Thomas, additional, Chinthrajah, R. Sharon, additional, Grifoni, Alba, additional, Sette, Alessandro, additional, Nadeau, Kari C., additional, Haslam, David B., additional, Bosinger, Steven E., additional, Wrammert, Jens, additional, Maecker, Holden T., additional, Utz, Paul J., additional, Wang, Taia T., additional, Khurana, Surender, additional, Khatri, Purvesh, additional, Staat, Mary A., additional, and Pulendran, Bali, additional

Published
2023
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35. Antibody Binding and Neutralization of Live SARS-CoV-2 Variants Including BA.4/5 Following Booster Vaccination of Patients with B-cell Malignancies

Author

Chang, Andres, primary, Akhtar, Akil, additional, Lai, Lilin, additional, Orellana-Noia, Victor M., additional, Linderman, Susanne L., additional, McCook-Veal, Ashley A., additional, Switchenko, Jeffrey M., additional, Saini, Manpreet, additional, Valanparambil, Rajesh M., additional, Blum, Kristie A., additional, Allen, Pamela B., additional, Lechowicz, Mary Jo, additional, Romancik, Jason T., additional, Ayers, Amy, additional, Leal, Alyssa, additional, O'Leary, Colin B., additional, Churnetski, Michael C., additional, Baird, Katelin, additional, Kives, Melissa, additional, Wrammert, Jens, additional, Nooka, Ajay K., additional, Koff, Jean L., additional, Dhodapkar, Madhav V., additional, Suthar, Mehul S., additional, Cohen, Jonathon B., additional, and Ahmed, Rafi, additional

Published
2022
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36. Antibody Response to COVID-19 mRNA Vaccine in Patients With Lung Cancer After Primary Immunization and Booster: Reactivity to the SARS-CoV-2 WT Virus and Omicron Variant

Author

Valanparambil, Rajesh M., primary, Carlisle, Jennifer, additional, Linderman, Susanne L., additional, Akthar, Akil, additional, Millett, Ralph Linwood, additional, Lai, Lilin, additional, Chang, Andres, additional, McCook-Veal, Ashley A., additional, Switchenko, Jeffrey, additional, Nasti, Tahseen H., additional, Saini, Manpreet, additional, Wieland, Andreas, additional, Manning, Kelly E., additional, Ellis, Madison, additional, Moore, Kathryn M., additional, Foster, Stephanie L., additional, Floyd, Katharine, additional, Davis-Gardner, Meredith E., additional, Edara, Venkata-Viswanadh, additional, Patel, Mit, additional, Steur, Conor, additional, Nooka, Ajay K., additional, Green, Felicia, additional, Johns, Margaret A., additional, O'Brein, Fiona, additional, Shanmugasundaram, Uma, additional, Zarnitsyna, Veronika I., additional, Ahmed, Hasan, additional, Nyhoff, Lindsay E., additional, Mantus, Grace, additional, Garett, Michael, additional, Edupuganti, Srilatha, additional, Behra, Madhusmita, additional, Antia, Rustom, additional, Wrammert, Jens, additional, Suthar, Mehul S., additional, Dhodapkar, Madhav V., additional, Ramalingam, Suresh, additional, and Ahmed, Rafi, additional

Published
2022
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37. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response

Author

Patel, Anamika, primary, Kumar, Sanjeev, additional, Lai, Lilin, additional, Chakravarthy, Chennareddy, additional, Valanparambil, Rajesh, additional, Reddy, Elluri Seetharami, additional, Gottimukkala, Kamalvishnu, additional, Bajpai, Prashant, additional, Raju, Dinesh Ravindra, additional, Edara, Venkata Viswanadh, additional, Davis-Gardner, Meredith E., additional, Linderman, Susanne, additional, Dixit, Kritika, additional, Sharma, Pragati, additional, Mantus, Grace, additional, Cheedarla, Narayanaiah, additional, Verkerke, Hans P., additional, Frank, Filipp, additional, Neish, Andrew S., additional, Roback, John D., additional, Davis, Carl W., additional, Wrammert, Jens, additional, Ahmed, Rafi, additional, Suthar, Mehul S., additional, Sharma, Amit, additional, Murali-Krishna, Kaja, additional, Chandele, Anmol, additional, and Ortlund, Eric A., additional

Published
2022
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38. Structural insights for neutralization of Omicron variants BA.1, BA.2, BA.4, and BA.5 by a broadly neutralizing SARS-CoV-2 antibody

Author

Kumar, Sanjeev, primary, Patel, Anamika, additional, Lai, Lilin, additional, Chakravarthy, Chennareddy, additional, Valanparambil, Rajesh, additional, Reddy, Elluri Seetharami, additional, Gottimukkala, Kamalvishnu, additional, Davis-Gardner, Meredith E., additional, Edara, Venkata Viswanadh, additional, Linderman, Susanne, additional, Nayak, Kaustuv, additional, Dixit, Kritika, additional, Sharma, Pragati, additional, Bajpai, Prashant, additional, Singh, Vanshika, additional, Frank, Filipp, additional, Cheedarla, Narayanaiah, additional, Verkerke, Hans P., additional, Neish, Andrew S., additional, Roback, John D., additional, Mantus, Grace, additional, Goel, Pawan Kumar, additional, Rahi, Manju, additional, Davis, Carl W., additional, Wrammert, Jens, additional, Godbole, Sucheta, additional, Henry, Amy R., additional, Douek, Daniel C., additional, Suthar, Mehul S., additional, Ahmed, Rafi, additional, Ortlund, Eric, additional, Sharma, Amit, additional, Murali-Krishna, Kaja, additional, and Chandele, Anmol, additional

Published
2022
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39. Loss of Pfizer (BNT162b2) Vaccine-Induced Antibody Responses against the SARS-CoV-2 Omicron Variant in Adolescents and Adults

Author

Gupta, Sneh Lata, primary, Mantus, Grace, additional, Manning, Kelly E., additional, Ellis, Madison, additional, Patel, Mit, additional, Ciric, Caroline Rose, additional, Lu, Austin, additional, Turner, Jackson S., additional, O’Halloran, Jane A., additional, Presti, Rachel M., additional, Joshi, Devyani Jaideep, additional, Ellebedy, Ali H., additional, Anderson, Evan J., additional, Rostad, Christina A., additional, Suthar, Mehul S., additional, and Wrammert, Jens, additional

Published
2022
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43. Epistasis reduces fitness costs of influenza A virus escape from stem-binding antibodies

Author

Lee, Chung-Young, primary, Caceres, C. Joaquin, additional, Geiger, Ginger, additional, Seibert, Brittany, additional, Faccin, Flavio Cargnin, additional, Gay, L. Claire, additional, Ferreri, Lucas M., additional, Kaul, Drishti, additional, Wrammert, Jens, additional, Tan, Gene S., additional, Perez, Daniel R., additional, and Lowen, Anice C., additional

Published
2022
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44. Structural insights for neutralization of BA.1 and BA.2 Omicron variants by a broadly neutralizing SARS-CoV-2 antibody

Author

Kumar, Sanjeev, primary, Patel, Anamika, additional, Lai, Lilin, additional, Chakravarthy, Chennareddy, additional, Valanparambil, Rajesh, additional, Davis-Gardner, Meredith E., additional, Edara, Venkata Viswanadh, additional, Linderman, Susanne, additional, Reddy, Elluri Seetharami, additional, Gottimukkala, Kamalvishnu, additional, Nayak, Kaustuv, additional, Bajpai, Prashant, additional, Singh, Vanshika, additional, Frank, Filipp, additional, Cheedarla, Narayanaiah, additional, Verkerke, Hans P., additional, Neish, Andrew S., additional, Roback, John D., additional, Mantus, Grace, additional, Goel, Pawan Kumar, additional, Rahi, Manju, additional, Davis, Carl W., additional, Wrammert, Jens, additional, Suthar, Mehul S., additional, Ahmed, Rafi, additional, Ortlund, Eric, additional, Sharma, Amit, additional, Murali-Krishna, Kaja, additional, and Chandele, Anmol, additional

Published
2022
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47. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains

Author

Dunand, Carole J. Henry, Leon, Paul E., Kaur, Kaval, Tan, Gene S., Zheng, Nai-Ying, Andrews, Sarah, Huang, Min, Qu, Xinyan, Huang, Yunping, Salgado-Ferrer, Marlene, Ho, Irvin Y., Taylor, William, Hai, Rong, Wrammert, Jens, Ahmed, Rafi, Garcia- Sastre, Adolfo, Palese, Peter, Krammer, Florian, and Wilson, Patrick C.

Subjects
Medical research, Medicine, Experimental, Avian influenza viruses -- Research, Monoclonal antibodies -- Physiological aspects, Health care industry
Abstract

The emergence and seasonal persistence of pathogenic H7N9 influenza viruses in China have raised concerns about the pandemic potential of this strain, which, if realized, would have a substantial effect on global health and economies. H7N9 viruses are able to bind to human sialic acid receptors and are also able to develop resistance to neuraminidase inhibitors without a loss in fitness. It is not clear whether prior exposure to circulating human influenza viruses or influenza vaccination confers immunity to H7N9 strains. Here, we demonstrate that 3 of 83 H3 HA-reactive monoclonal antibodies generated by individuals that had previously undergone influenza A virus vaccination were able to neutralize H7N9 viruses and protect mice against homologous challenge. The H7N9-neutralizing antibodies bound to the HA stalk domain but exhibited a difference in their breadth of reactivity to different H7 influenza subtypes. Mapping viral escape mutations suggested that these antibodies bind at least two different epitopes on the stalk region. Together, these results indicate that these broadly neutralizing antibodies may contribute to the development of therapies against H7N9 strains and may also be effective against pathogenic H7 strains that emerge in the future., Introduction Influenza A viruses evade the human immune system by changing the antigenic regions of their surface glycoproteins using two mechanisms: antigenic drift (point mutations) and antigenic shift (gene segment [...]

Published
2015
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48. Epistasis reduces fitness costs of influenza A virus escape from stem-binding antibodies.

Author

Chung-Young Lee, Raghunathan, Vedhika, Caceres, C. Joaquin, Geiger, Ginger, Seibert, Brittany, Faccin, Flavio Cargnin, Gay, L. Claire, Ferreri, Lucas M., Kaul, Drishti, Wrammert, Jens, Tan, Gene S., Perez, Daniel R., and Lowen, Anice C.

Subjects
INFLUENZA A virus, INFLUENZA viruses, IMMUNOGLOBULINS, RECOMBINANT viruses, MEMBRANE fusion, RECREATION centers
Abstract

The hemagglutinin (HA) stem region is a major target of universal influenza vaccine efforts owing to the presence of highly conserved epitopes across multiple influenza A virus (IAV) strains and subtypes. To explore the potential impact of vaccine-induced immunity targeting the HA stem, we examined the fitness effects of viral escape from stem-binding broadly neutralizing antibodies (stem-bnAbs). Recombinant viruses containing each individual antibody escape substitution showed diminished replication compared to wild-type virus, indicating that stem-bnAb escape incurred fitness costs. A second-site mutation in the HA head domain (N129D; H1 numbering) reduced the fitness effects observed in primary cell cultures and likely enabled the selection of escape mutations. Functionally, this putative permissive mutation increased HA avidity for its receptor. These results suggest a mechanism of epistasis in IAV, wherein modulating the efficiency of attachment eases evolutionary constraints imposed by the requirement for membrane fusion. Taken together, the data indicate that viral escape from stem-bnAbs is costly but highlights the potential for epistatic interactions to enable evolution within the functionally constrained HA stem domain. [ABSTRACT FROM AUTHOR]

Published
2023
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