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Fagopyri Dibotryis Rhizoma, a traditional Chinese medicine, is widely used to treat various ailments such as pulmonary abscesses, measles pneumonia, and swelling. Its notable therapeutic effects are closely related to the chemical constituents, making it crucial to conduct in‐depth research on the chemical components. In this study, a total of 209 compounds were preliminarily identified using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry in Fagopyri Dibotryis Rhizoma. Subsequently, a method was established using ultra‐high‐performance liquid chromatography‐triple quadrupole/linear ion trap tandem mass spectrometry for the simultaneous determination of 24 main ingredients (flavonoids and phenolic acids) in Fagopyri Dibotryis Rhizoma from different habitats and growing periods. Quantitative results showed that different compounds displayed varying contents across different habitats, with catechin, epicatechin, procyanidin B2, and procyanidin C1 being relatively abundant. Principal component analysis and partial least squares discriminant analysis showed there were significant differences among Fagopyri Dibotryis Rhizoma from different habitats, and the quality from Yunnan was superior. Entropy weight TOPSIS analysis showed that Fagopyri Dibotryis Rhizoma grown until late October has better comprehensive quality, which is basically consistent with the traditional harvesting time. In summary, this study can provide reference methods for the comprehensive evaluation of the quality of Fagopyri Dibotryis Rhizoma. [ABSTRACT FROM AUTHOR]

Domestication has shaped the population structure and agronomic traits of tea plants, yet the complexity of tea population structure and genetic variation that determines these traits remains unclear. We here investigated the resequencing data of 363 diverse tea accessions collected extensively from almost all tea distributions and found that the population structure of tea plants was divided into eight subgroups, which were basically consistent with their geographical distributions. The genetic diversity of tea plants in China decreased from southwest to east as latitude increased. Results also indicated that Camellia sinensis var. assamica (CSA) illustrated divergent selection signatures with Camellia sinensis var. sinensis (CSS). The domesticated genes of CSA were mainly involved in leaf development, flavonoid and alkaloid biosynthesis, while the domesticated genes in CSS mainly participated in amino acid metabolism, aroma compounds biosynthesis, and cold stress. Comparative population genomics further identified ~730 Mb novel sequences, generating 6,058 full‐length protein‐encoding genes, significantly expanding the gene pool of tea plants. We also discovered 217,376 large‐scale structural variations and 56,583 presence and absence variations (PAVs) across diverse tea accessions, some of which were associated with tea quality and stress resistance. Functional experiments demonstrated that two PAV genes (CSS0049975 and CSS0006599) were likely to drive trait diversification in cold tolerance between CSA and CSS tea plants. The overall findings not only revealed the genetic diversity and domestication of tea plants, but also underscored the vital role of structural variations in the diversification of tea plant traits. [ABSTRACT FROM AUTHOR]

Natural gas hydrate(NGH) is a clean resource with huge reserves. The depressurization method is an economical and effective exploitation method. In the process of depressurization, reservoir absolute permeability has an important influence on production results. Based on the data of Shenhu hydrate reservoirs, this paper established a depressurization production numerical simulation model. Then, the production performances such as pressure, temperature, gas production rate, cumulative gas production, and hydrate dissociation effect are all studied under different permeability conditions.study the change of reservoir pressure, gas production rate, cumulative gas production, reservoir temperature change and hydrate dissociation effect under different permeability conditions. Results show that higher permeability is conducive to the depressurization of hydrate reservoirs.

Background: The molecular mechanism that controls the activation of Cyclin B1-CDK1 complex has been widely investigated. It is generally believed that CDC25B acts as a 'starter phosphatase' of mitosis. In this study, we investigate the sequential regulation of meiotic resumption by CDC25B and Cyclin B1 in mouse oocytes. Results: Injection of mRNAs coding for CDC25B-Ser351A and/or Cyclin B1-Ser123A shows a more potent maturation-inhibiting ability than their respective wild type. Co-injection of mRNAs coding for phosphor-mimic CDC25B-Ser351D and Cyclin B1-Ser123D can rescue this prophase I arrest induced by CDC25B-Ser351A or Cyclin B1-Ser123A. In addition, p-CDC25B-Ser351 is co-localized at the microtubule-organizing centers (MTOCs) with Aurora kinase A (AURKA) during maturation and p-Cyclin B1-Ser123 is only captured on MTOCs shortly before germinal vesicle breakdown (GVBD). Depletion of AURKA not only resulted in metaphase I (MI) spindle defects and anaphase I (AI) abnormal chromosomes separation but also prevented the phosphorylation of CDC25B-Ser351 at centrosomes. AURKA depletion induced deficiencies of spindle assembly and progression to MII can be rescued by CDC25B-Ser351D mRNA injection. Conclusions: AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. Developmental Dynamics 244:110-121, 2015. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

The invasion of formation water during marine hydrate production gravely affects gas productivity. CO 2 hydrate caps effectively inhibit water invasion while sequestering CO 2 , which is considered an excellent prospective application. However, forming field-scale artificial CO 2 hydrate caps remains a global challenge. This study proposed an innovative method for forming an artificial CO 2 hydrate cap. The fracture length, injection time, production pressure, and production well distance, which are all factors that affect sealing capacity and production enhancement, were optimized. Results indicated that the sealing capacity exhibited a trend of initially increasing and subsequently decreasing with fracture length. The injection time was positively correlated with the sealing capacity and was sufficient to seal both vertically and horizontally once it reached 4 years. In addition to sequestering CO 2 , the CO 2 hydrate cap enhanced gas production by 146.56 % and reduced water production by 37.47 % compared to direct depressurization. CO 2 invasion occurred when the well spacing was too close, while being distant was not favorable for pressure propagation and heat utilization by the CO 2 hydrate cap. This method simultaneously achieves multiple goals for energy and environmental, providing essential implications for the future commercial application of marine hydrates. [Display omitted] • A novel method for forming field-scale CO 2 hydrate cap is proposed. • Optimal fracture length and injection time are suggested for sufficient sealing cap. • Favorable production well spacing under the CO 2 hydrate cap is revealed. • Sequestering CO 2 while increasing gas production and decreasing water production. [ABSTRACT FROM AUTHOR]

The purpose of this study is to reveal the role of Girdin in regulating the aggregation of actin filaments by studying the relationship between PKB/Akt and Girdin. First we used Scansite software (http://scansite.mit.edu) to predict relevant target sites of PKB/Akt on mouse Girdin. To gain insight into the role of phosphorylation of Girdin by PKB/Akt, we assessed the location of phosphorylated Girdin in fertilized eggs by staining with anti-P-Girdin 1 417 Ab. We detected a distinct increase in the fluorescence signal of F-actin and P-Girdin 1 417 after microinjection of Akt WT and myr-Akt. The addition of myr-Akt induced phosphorylation of Girdin in mouse fertilized eggs. In addition, siRNA-mediated Akt-knockdown blocked phosphorylation of Girdin. The distribution of actin filaments was obviously scattered. These results strongly suggest that PKB/Akt could directly phosphorylate Girdin on Ser1 417 and promote its function in mouse fertilized eggs.