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277 results on '"Wu, Fang-Ying"'

13. Confining Thiolysis of Dinitrophenyl Ether to a Luminescent Metal–Organic Framework with a Large Stokes Shift for Highly Efficient Detection of Hydrogen Sulfide in Rat Brain

Author

Qin, Mengxia, Ji, Wenliang, Huang, Pengcheng, Wu, Fang-Ying, and Mao, Lanqun

Abstract

Hydrogen sulfide (H2S) is a gaseous signaling molecule that regulates various physiological and pathological processes in the central nervous system. It is vital to develop an effective method to detect H2S in vivo to elucidate its critical role. However, current fluorescent probes for accurate quantification of H2S still face big challenges due to complicated fabrication, small Stokes shift, unsatisfactory selectivity, and especially delayed response time. Herein, based on simple postsynthetic modification, we present an innovative strategy by confining H2S-triggered thiolysis of dinitrophenyl (DNP) ether within a luminescent metal–organic framework (MOF) to address those issues. Due to the cleavage of the DNP moiety by H2S, the nanoprobe gives rise to a remarkable fluorescence turn-on signal with a large Stokes shift of 190 nm and also provides high selectivity to H2S against various interferents including competing biothiols. In particular, by virtue of the unique structural property of the MOF, it exhibits an ultrafast sensing ability for H2S (only 5 s). Moreover, the fluorescence enhancement efficiency displays a good linear correlation with H2S concentration in the range of 0–160 μM with a detection limit of 0.29 μM. Importantly, these superior sensing performances enable the nanoprobe to measure the basal value and monitor the change of H2S level in the rat brain.

Published
2024
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19. Molecular Network-Bearing Fe–N–C Single-Atom Nanozymes for Monitoring Intracellular Glutathione Fluctuations.

Author

Cai, Chunqi, Chen, Wen, Zhu, Chengyang, Huang, Pengcheng, Wu, Fang-Ying, Deng, Ke-Yu, and Mao, Junjie

Abstract

Nanozymes have drawn much attention owing to their low cost and high stability compared with natural enzymes. However, limited specificity intrinsically presents a challenge to their efficiency in sensing applications. Although specific biorecognition units and molecularly imprinted polymers have been introduced to enhance the specificity of nanozymes, they have some shortcomings like poor stability, high cost, complicated operation, and weakened activity. To address these issues, we developed here a simple and efficient surface engineering strategy to build the target-responsive sensing interface by weaving a molecular network on nanozymes to afford a pocket-like structure. Glutathione (GSH), an important intracellular biothiol, was used as the model target, considering the difficulty in distinguising GSH from other biothiols, especially cysteine (Cys) and homocysteine (Hcy). With high-performance single-atom nanozyme (SAzyme) Fe–N–C as the parent nanozyme, a meshy SAzyme Fe/PSAs-DTSSP containing GSH-cleavable disulfide bonds was fabricated via facile surface modification. Interestingly, the molecular network adhered onto the Fe–N–C surface modulated the peroxidase-like properties, including substrate affinity and catalytic stability under harsh conditions. Moreover, the molecular network could specifically respond to GSH over Cys, Hcy, and other interferents, which suppressed the oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) due to the reversion of the surface charge property of Fe/PSAs-DTSSP, resulting in a sensitive response identified by the naked eye. The usefulness of this assay was validated by monitoring the fluctuation of the GSH level in cancer cells treated with glucose deprivation or incubated with doxorubicin, showing good accuracy and reliability. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Distinct dual enzyme-like activities of Fe–N–C single-atom nanozymes enable discriminative detection of cellular glutathione.

Author

Cai, Chunqi, Zhu, Chengyang, Lv, Lingling, Huang, Pengcheng, Mao, Junjie, Wu, Fang-Ying, and Deng, Ke-Yu

Subjects
SYNTHETIC enzymes, REACTIVE oxygen species, GLUTATHIONE
Abstract

Fe–N–C single-atom nanozymes readily achieved discriminative detection of glutathione (GSH) over other biothiols with similar structure due to the difference between POD-like and OXD-like activities regarding the kind of reactive oxygen species. This colorimetric sensor demonstrated the heterogeneity of GSH levels in different cells and accurately monitored cellular GSH fluctuation. [ABSTRACT FROM AUTHOR]

Published
2023
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