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Context: Maternal-effect genes (MEGs) play a critical role in modulating both cellular and molecular biology events in preimplantation embryonic development. Damage-specific DNA binding protein 1 (DDB1) is a gene that participates in meiotic resumption, ovulation, and embryonic stem cell maintenance. Its function in preimplantation development is not well-studied. Aims: We aimed to explore the expression pattern, genomic heritage, and potential molecular mechanisms of DDB1 in preimplantation embryos in porcine. Methods: In this study, RNA interference, microinjection, RT-qPCR, immunofluorescence staining and single-cell RNA sequencing were used to explore the molecular function of DDB1 in porcine preimplantation embryos. Key results: DDB1 was found to be expressed in germinal vesicle (GV) and Meiosis II (MII) oocytes and in preimplantation embryos. We confirmed it is a MEG. DDB1 -deficient blastocysts had a significantly reduced number of trophectoderm cells, an increased apoptotic cell number and increased apoptosis index. According to a next-generation sequencing (NGS) analysis, 236 genes (131 upregulated and 105 downregulated) significantly changed in the DDB1 -deficient morula. The myeloid leukaemia factor 1 (MLF1) and yes-associated protein 1 (YAP1) expressions were significantly upregulated and downregulated respectively, in the DDB1 -deficient morula. In combination with the decreased expression of TEAD4 , CDX2 , GATA3 , OCT4 , and NANOG and the increased expression of SOX2 in the blastocyst, DDB1 may play a role in determining lineage differentiation and pluripotency maintenance. Conclusions: DDB1 is a MEG and it plays a crucial role in porcine preimplantation embryonic development. Implications: This study provides a theoretical basis for further understanding the molecular mechanisms of preimplantation embryo development. DDB1 is a gene that participates in meiotic resumption, ovulation, and embryonic stem cell maintenance. It is produced from maternal origin transcripts in early porcine embryos. DDB1 -deficiency enhanced cellular apoptosis in the blastocyst. Lack of DDB1 impaired trophectoderm formation and the pluripotency maintenance of blastocyst. DDB1 is a MEG, which plays a the crucial role of in porcine preimplantation embryonic development. [ABSTRACT FROM AUTHOR]

Objective: To explore whether thoracic endovascular aortic repair (TEVAR) plus distal bare metal stent (BMS) implantation leads to favorable clinical outcomes compared with standard TEVAR in treating acute complicated type B aortic dissection. Methods: Relevant publications were found through a precise search of PubMed, Cochrane Library, and EMBASE. Count data were calculated as the odd ratio (OR)and 95% confidence interval (CI) using the Mantel–Haenszel statistical method, quantitative data were calculated as mean difference and 95% CI using Inverse Variance statistical method. When the data heterogeneity was large, with an I2 > 50%, a random-effects model and sensitivity analysis were performed. The analysis tool we used was the software Revman 5.3 and G*power 3.1. Results: There were 7 publications involving 958 patients who were enrolled ultimately. The incidence of unplanned secondary intervention and postoperative adverse events in the TEVAR + BMS were lower than standard TEVAR (OR, 0.42, (95% CI, 0.23 to 0.75); OR, 0.57, (95% CI, 0.37 to 0.90)), and the pooled number needed to treat was 15 for unplanned secondary intervention and 15 for postoperative adverse events. There were no significant difference in the aortic-related or all-cause 30-day mortality (OR, 0.81, (95% CI, 0.25 to 2.61); OR, 0.47, (95% CI, 0.18 to 1.22)), aortic-related, all-cause mortality at least 6 months or incidence of the postoperative endoleak (OR, 0.47, (95% CI, 0.17 to 1.32); OR, 0.42, (95% CI, 0.17 to 1.06); OR, 0.81, (95% CI, 0.32 to 2.05)). Conclusion: There is no significant outcome difference except for reduced reintervention but this does not seem to adversely affect survival. It is unclear whether this justifies the additional cost of placing it in every complicated type B aortic dissection regardless of anatomy after standard TEVAR alone. Besides, more data are needed to verify the adjunctive distal bare metal stents' performance at different dissection stages. [ABSTRACT FROM AUTHOR]

Northern snakehead (Channa argus) is very popular freshwater fish in China and is expected to become a new surimi material with therapeutic efficacy. The Angelica sinensis powder is a type of famous Chinese medicine that helps to regulate metabolism and enhance the immune function. Therefore, an upmarket medicated surimi product with hygienic qualities was designed to adapt to the stressful current lifestyle using snakehead and Angelica sinensis powder. The texture, whiteness, rheological properties, and sensory properties were studied to further optimize the dosage of the Angelica sinensis powder used in the surimi processing. The results indicated that the maximum breaking force and breaking distance were obtained at a concentration of 4 g/kg, and the whiteness value and driage were significantly decreased with the increase of the amount of the Angelica sinensis powder. Ultimately, the highest total sensory evaluation score was obtained for samples with the addition of 2 g/kg Angelica sinensis powder. This study shows that the Angelica sinensis powder has the potentiality to become an added ingredient to snakehead surimi products in order to improve the health status of sub-health population. [ABSTRACT FROM AUTHOR]