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145 results on '"Wu, Hong-Lin"'

11. The role of KDM4A‐mediated histone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2 axis.

Author

Li, Xi‐Xi, Xu, Jia‐Kun, Su, Wei‐Jie, Wu, Hong‐Lin, Zhao, Kun, Zhang, Chang‐Ming, Chen, Xiang‐Kun, and Yang, Li‐Xuan

Subjects
HISTONE methylation, GLIOMAS, TEMOZOLOMIDE, METHYLGUANINE, CELL survival, UBIQUITINATION
Abstract

Temozolomide (TMZ) resistance presents a significant challenge in the treatment of gliomas. Although lysine demethylase 4A (KDM4A) has been implicated in various cancer‐related processes, its role in TMZ resistance remains unclear. This study aims to elucidate the contribution of KDM4A to TMZ resistance in glioma cells and its potential implications for glioma prognosis. We assessed the expression of KDM4A in glioma cells (T98G and U251MG) using qRT‐PCR and Western blot assays. To explore the role of KDM4A in TMZ resistance, we transfected siRNA targeting KDM4A into drug‐resistant glioma cells. Cell viability was assessed using the CCK‐8 assay and the TMZ IC50 value was determined. ChIP assays were conducted to investigate KDM4A, H3K9me3, and H3K36me3 enrichment on the promoters of ROCK2 and HUWE1. Co‐immunoprecipitation confirmed the interaction between HUWE1 and ROCK2, and we examined the levels of ROCK2 ubiquitination following MG132 treatment. Notably, T98G cells exhibited greater resistance to TMZ than U251MG cells, and KDM4A displayed high expression in T98G cells. Inhibiting KDM4A resulted in decreased cell viability and a reduction in the TMZ IC50 value. Mechanistically, KDM4A promoted ROCK2 transcription by modulating H3K9me3 levels. Moreover, disruption of the interaction between HUWE1 and ROCK2 led to reduced ROCK2 ubiquitination. Inhibition of HUWE1 or overexpression of ROCK2 counteracted the sensitization effect of si‐KDM4A on TMZ responsiveness in T98G cells. Our findings highlight KDM4A's role in enhancing TMZ resistance in glioma cells by modulating ROCK2 and HUWE1 transcription and expression through H3K9me3 and H3K36me3 removal. [ABSTRACT FROM AUTHOR]

Published
2024
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14. The role of KDM4A‐mediatedhistone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2axis

Author

Li, Xi‐Xi, Xu, Jia‐Kun, Su, Wei‐Jie, Wu, Hong‐Lin, Zhao, Kun, Zhang, Chang‐Ming, Chen, Xiang‐Kun, and Yang, Li‐Xuan

Abstract

Temozolomide (TMZ) resistance presents a significant challenge in the treatment of gliomas. Although lysine demethylase 4A (KDM4A) has been implicated in various cancer‐related processes, its role in TMZ resistance remains unclear. This study aims to elucidate the contribution of KDM4A to TMZ resistance in glioma cells and its potential implications for glioma prognosis. We assessed the expression of KDM4A in glioma cells (T98G and U251MG) using qRT‐PCR and Western blot assays. To explore the role of KDM4A in TMZ resistance, we transfected siRNA targeting KDM4A into drug‐resistant glioma cells. Cell viability was assessed using the CCK‐8 assay and the TMZ IC50 value was determined. ChIP assays were conducted to investigate KDM4A, H3K9me3, and H3K36me3 enrichment on the promoters of ROCK2 and HUWE1. Co‐immunoprecipitation confirmed the interaction between HUWE1 and ROCK2, and we examined the levels of ROCK2 ubiquitination following MG132 treatment. Notably, T98G cells exhibited greater resistance to TMZ than U251MG cells, and KDM4A displayed high expression in T98G cells. Inhibiting KDM4A resulted in decreased cell viability and a reduction in the TMZ IC50 value. Mechanistically, KDM4A promoted ROCK2 transcription by modulating H3K9me3 levels. Moreover, disruption of the interaction between HUWE1 and ROCK2 led to reduced ROCK2 ubiquitination. Inhibition of HUWE1 or overexpression of ROCK2 counteracted the sensitization effect of si‐KDM4A on TMZ responsiveness in T98G cells. Our findings highlight KDM4A's role in enhancing TMZ resistance in glioma cells by modulating ROCK2 and HUWE1 transcription and expression through H3K9me3 and H3K36me3 removal.

Published
2024
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25. Transsphenoidal surgery for prolactinomas in male patients: a retrospective study.

Author

Su, Wei-Jie, Cai, Hong-Cai, Yang, Guo-Chen, He, Ke-Jun, Wu, Hong-Lin, Yang, Yi-Bing, Tang, Hong-Xing, Yang, Li-Xuan, and Deng, Chun-Hua

Abstract

Male patients with prolactinomas usually present with typical hyperprolactinemia symptoms, including sexual dysfunction and infertility. However, clinical factors related to sexual dysfunction and surgical outcomes in these patients remain unclear. This study aimed to investigate the outcomes of male patients with prolactinomas after transsphenoidal surgery and the risk factors affecting sexual dysfunction. This study was conducted on 58 male patients who underwent transsphenoidal surgery for prolactinomas between May 2014 and December 2020 at the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. We evaluated the sexual function of patients before and after surgery through International Index of Erectile Function-5 scores, libido, and frequency of morning erection. Of the 58 patients, 48 (82.8%) patients had sexual intercourse preoperatively. Among those 48 patients, 41 (85.4%) patients presented with erectile dysfunction. The preoperative International Index of Erectile Function-5 scores in patients with macroprolactinomas were significantly higher than those in patients with giant prolactinomas (17.63 ± 0.91 vs 13.28 ± 1.43; P = 0.01). Postoperatively, the incidence of erectile dysfunction was 47.9%, which was significantly lower than that preoperatively (85.4%; P = 0.01). Twenty-eight (68.3%) patients demonstrated an improvement in erectile dysfunction. Tumor size and invasiveness were significantly correlated with the improvement of erectile dysfunction. Preoperative testosterone <2.3 ng ml−1 was an independent predictor of improvement in erectile dysfunction. In conclusion, our results indicated that tumor size and invasiveness were important factors affecting the improvement of sexual dysfunction in male patients with prolactinoma. The preoperative testosterone level was an independent predictor related to the improvement of erectile dysfunction. [ABSTRACT FROM AUTHOR]

Published
2023
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47. A Sentence Alignment Model Based on Combined Clues and Kernel Extensional Matrix Matching Method

Author

Wu Hong-lin, Liu Yi-yang, and Liu Shao-ming

Subjects
Matching (graph theory), business.industry, Word error rate, Pattern recognition, General Medicine, Construct (python library), Lexicon, Extensional definition, Matrix (mathematics), Kernel (image processing), Artificial intelligence, business, Sentence, Mathematics
Abstract

A sentence alignment model based on combined clues and Kernel Extensional Matrix Matching (KEMM) method is proposed. In this model, a similarity matrix for sentence aligning is formed by the similarities of bilingual sentences calculated by the combined clues, such as lexicon, morphology, length and special symbols, etc.; then this similarity matrix is used to construct a select matrix for sentence aligning; finally, obtains the sentence alignments by KEMM. Experimental results illustrated that our model outperforms over the Gale's system on handling any types of sentence alignments, with 30% total sentence alignment error rate decreasing.

Published
2012

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