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Simple Summary: Porcine epidemic diarrhea (PED) is caused by a coronavirus that poses a serious threat to the global pig industry. Black soldier fly is a very high-quality protein raw material that could serve as a functional feed additive to improve intestinal health in piglets. The study aimed to evaluate the effects of black soldier fly extract (BFE) on growth performance, plasma biochemical parameters, antioxidant capacity and antiviral genes in PEDV-infected piglets. The results showed that the addition of 500 mg/kg BW BFE to the diet could improve the intestinal function of piglets and activate the immune system after PEDV infection. Porcine epidemic diarrhea virus (PEDV) has developed as a global problem for the pig business, resulting in significant financial losses. Black soldier fly extract (BFE) has been proven to improve intestinal growth in pigs after weaning. Consequently, the goal of the present investigation was to explore the effects of BFE supplementation on intestinal function in PEDV-infected piglets. Eighteen piglets were randomly allocated to three groups: control, PEDV, and BFE + PEDV. The piglets in the BFE + PEDV group received 500 mg/kg BW of BFE orally for seven days from day 4 to 10 of the study. On day 9 of the study, six pigs from each group received either clean saline or PEDV solution at a dosage of 106 TCID50 (50% tissue culture infectious dose) per pig. On day 11, samples of blood and intestine were taken for additional investigation. The results indicated a significant decrease in the average daily gain (ADG) of piglets infected with PEDV (p < 0.05). Additionally, PEDV infection led to an alteration of blood indexes and a reduction in plasma D-xylose concentration and villi height in the small intestine, while it increased plasma diamine oxidase activity and small intestinal crypt depth in piglets (p < 0.05). The PEDV infection significantly reduced antioxidant enzyme activity in plasma and the gut, including total superoxide dismutase and catalase, while increasing contents of oxidation-relevant products such as malondialdehyde and hydrogen peroxide in piglets. Moreover, PEDV infection increased the mRNA expression level of antiviral-related genes (p < 0.05). Nutritional supplementation with BFE improved intestinal histomorphological indicators and reduced oxidative stress produced by PEDV infection in piglets. Interestingly, BFE could significantly promote the mRNA expression level of antiviral-related genes in the ileum (p < 0.05). Overall, the preliminary results suggest that dietary BFE could improve intestinal function in piglets after PEDV infection. Currently, the findings put a spotlight on the role of BFE in the prevention and treatment of PED in piglets. [ABSTRACT FROM AUTHOR]

The most commonly used real-time augmentation services in China are the International GNSS Service's (IGS) real-time service (RTS), PPP-B2b service, and Double-Frequency Multi-Constellation (DFMC) service of the BeiDou Satellite-Based Augmentation System (BDSBAS) service. However, research on the performance evaluation, comparison, and application scope of these three products is still incomplete. This article introduces methods for obtaining real-time augmentation information and real-time orbit and clock offset recovery. Based on real-time orbit and clock offset accuracy, positioning accuracy, and positioning availability, this article systematically evaluates the performance and analyzes the application fields of Centre National d'Études Spatiales (CNES), PPP-B2b, and BDSBAS augmentation information. The results of the evaluation revealed that the radial accuracy of the CNES and PPP-B2b real-time orbit product is consistent, and the Root Mean Square (RMS) is better than 5 cm. The CNES real-time orbit product can achieve centimeter-level accuracy in both along-track and cross-track components, surpassing PPP-B2b's decimeter-level accuracy. Both services demonstrate consistent accuracy in the real-time clock offset, with PPP-B2b showing similar standard deviations (STDs) of 0.16 ns for different satellites. However, for CNES, the STD of the real-time clock offset varies, with values of 0.10 ns, 0.19 ns, and 0.60 ns, respectively, for GPS, BDS-3 Medium Earth Orbit (MEO), and BDS-3 Inclined Geosynchronous Satellite Orbit (IGSO) satellites. Centimeter-level accuracy is achieved after convergence and positioning availability exceeds 99% for CNES and PPP-B2b services. Therefore, the difference between the two services in application areas depends on the acquisition of augmentation information. However, BDSBAS, which concentrates on code observations, demonstrates inferior performance in real-time orbit, clock offset, positioning accuracy, and positioning availability when compared to the other two services. Its primary application is in the aviation and maritime domains, where there is a greater need for service integrity, continuity, and reliability. [ABSTRACT FROM AUTHOR]