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9 results on '"Wu, Pu-yuan"'

3. Profiles of genomic alterations in primary esophageal follicular dendritic cell sarcoma

Author

Ren, Wei, Sun, Qi, Wu, Pu-Yuan, Huang, Bin, Yang, Ju, Yan, Jing, and Liu, Bao-Rui

Subjects
esophagus, sarcoma, Esophageal Neoplasms, Dendritic Cell Sarcoma, Follicular, chemotherapy, Treatment Refusal, Fatal Outcome, Lymphatic Metastasis, Positron Emission Tomography Computed Tomography, follicular dendritic cell sarcoma, Mutation, Biomarkers, Tumor, Humans, Female, Clinical Case Report, immunotherapy, radiotherapy, Research Article, Aged
Abstract

Rationale: Follicular dendritic cell (FDC) sarcoma is a rare tumor with FDC differentiation that typically arises within lymph nodes but can also occur extranodally. To date, the primary esophageal FDC sarcoma has not been reported in the English literature. Patient concerns: We described a 67-year-old female who foremostly presented with dysphagia, and the patient was readmitted due to a dry cough and pain of his right shoulder 2 years after initial treatment. Diagnoses: Primary esophageal FDC sarcoma with the right superior mediastinal lymph node metastasis. Interventions: The esophageal tumor was removed by endoscopic submucosal dissection at the first hospitalization. At the second hospitalization 2 years after the initial visit, the tracheal stent loaded with (125) iodine radioactive seeds was placed. The profiles of genetic variations and immunotherapeutic biomarkers were also explored by next-generation sequencing protocol from the patient's blood, esophageal primary, and mediastinal metastatic tumor samples. Outcomes: The patient's symptom transitorily relieved, but she gave up further treatment and died 2 months after the tracheal stent was placed. As for the genomic alterations, we found 9 gene mutations in all the samples, including checkpoint kinase 2(CHEK2), FAT atypical cadherin 1 (FAT1), tumor protein 53 (TP53), DPYD, ERBB2 interacting protein (ERBB2IP), FBXW7, KMT2D, PPP2R1A, TSC2, whereas amplification of MYC was only in the metastatic example. The analysis of clonal evolution and phylogenetic tree showed the propagation and replay of polyclonal esophageal FDC sarcoma. At the same time, the detection of biomarkers for immunotherapy revealed microsatellite stable and mismatch repair-proficient (pMMR), which predicted a relatively poor anti-programmed death (PD-1)/programmed death ligand (PD-L1) immunotherapy outcome. On the contrary, the tumor mutational burdens were 10 mutations per 1 million bases in both the primary and metastatic tumor sample, which ranked the top 23.3% in solid tumors mutational burdens database of Geneseeq and might be a good predictor of the efficacy of anti-PD-1/PD-L1 immunotherapy. Lessons: To the best of our knowledge, this case report announced the first case of extranodal primary esophageal FDC sarcoma in the world, and firstly revealed its unique genetic alterations profiles, which might contribute to further in-depth study of this rare disease.

Published
2018

5. Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells

Author

Cui,Fang-bo, Liu,Qin, Li,Ru-Tian, Shen,Jie, Wu,Pu-yuan, Yu,Li-Xia, Hu,Wen-jing, Wu,Feng-lei, Jiang,Chun-Ping, Yue,Guo-feng, Qian,Xiao-Ping, Jiang,Xi-Qun, Liu,Bao-Rui, Cui,Fang-bo, Liu,Qin, Li,Ru-Tian, Shen,Jie, Wu,Pu-yuan, Yu,Li-Xia, Hu,Wen-jing, Wu,Feng-lei, Jiang,Chun-Ping, Yue,Guo-feng, Qian,Xiao-Ping, Jiang,Xi-Qun, and Liu,Bao-Rui

Abstract

Fang-bo Cui,1,* Qin Liu,1,* Ru-Tian Li,1 Jie Shen,1 Pu-yuan Wu,1 Li-Xia Yu,1 Wen-jing Hu,1 Feng-lei Wu,2 Chun-Ping Jiang,1 Guo-feng Yue,2 Xiao-Ping Qian,1 Xi-Qun Jiang,3 Bao-Rui Liu11The Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, 2Nanjing Medical University, 3Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing, People's Republic of China*These authors contributed equally to this workAbstract: Radiotherapy is the main locoregional control modality for many types of unresectable tumors, including gastric cancer. However, many patients fail radiotherapy due to intrinsic radioresistance of cancer cells, which has been found to be strongly associated with cancer stem cell (CSC)-like properties. In this study, we developed a nanoparticle formulation to deliver miR-200c, which is reported to inhibit CSC-like properties, and then evaluated its potential activity as a radiosensitizer. miR-200c nanoparticles significantly augmented radiosensitivity in three gastric cancer cell lines (sensitization enhancement ratio 1.13–1.25), but only slightly in GES-1 cells (1.06). In addition to radioenhancement, miR-200c nanoparticles reduced the expression of CD44, a putative CSC marker, and the percentage of CD44+ BGC823 cells. Meanwhile, other CSC-like properties, including invasiveness and resistance to apoptosis, could be suppressed by miR-200c nanoparticles. CSC-associated radioresistance mechanisms, involving reactive oxygen species levels and DNA repair capacity, were also attenuated. We have demonstrated that miR-200c nanoparticles are an effective radiosensitizer in gastric cancer cells and induce little radiosensitization in normal cells, which suggests that they are as a promising candidate for further preclinical and clinical evaluation.Keywords

Published
2014

8. Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells.

Author

Cui FB, Liu Q, Li RT, Shen J, Wu PY, Yu LX, Hu WJ, Wu FL, Jiang CP, Yue GF, Qian XP, Jiang XQ, and Liu BR

Subjects
Cell Line, Tumor, Escherichia coli Proteins, Humans, Nanocapsules administration & dosage, Nanocapsules ultrastructure, Nanocomposites administration & dosage, Nanocomposites chemistry, Nanocomposites statistics & numerical data, Neoplastic Stem Cells drug effects, Neoplastic Stem Cells radiation effects, Peptides administration & dosage, Stomach Neoplasms genetics, Gelatinases metabolism, Nanocapsules chemistry, Peptides pharmacokinetics, Polyesters chemistry, Polyethylene Glycols chemistry, Radiation-Sensitizing Agents administration & dosage, Stomach Neoplasms metabolism, Stomach Neoplasms radiotherapy
Abstract

Radiotherapy is the main locoregional control modality for many types of unresectable tumors, including gastric cancer. However, many patients fail radiotherapy due to intrinsic radioresistance of cancer cells, which has been found to be strongly associated with cancer stem cell (CSC)-like properties. In this study, we developed a nanoparticle formulation to deliver miR-200c, which is reported to inhibit CSC-like properties, and then evaluated its potential activity as a radiosensitizer. miR-200c nanoparticles significantly augmented radiosensitivity in three gastric cancer cell lines (sensitization enhancement ratio 1.13-1.25), but only slightly in GES-1 cells (1.06). In addition to radioenhancement, miR-200c nanoparticles reduced the expression of CD44, a putative CSC marker, and the percentage of CD44(+) BGC823 cells. Meanwhile, other CSC-like properties, including invasiveness and resistance to apoptosis, could be suppressed by miR-200c nanoparticles. CSC-associated radioresistance mechanisms, involving reactive oxygen species levels and DNA repair capacity, were also attenuated. We have demonstrated that miR-200c nanoparticles are an effective radiosensitizer in gastric cancer cells and induce little radiosensitization in normal cells, which suggests that they are as a promising candidate for further preclinical and clinical evaluation.

Published
2014
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9. [Germ-line epimutations and human cancer].

Author

Wu PY, Fan YM, and Wang YP

Subjects
Adaptor Proteins, Signal Transducing metabolism, Animals, BRCA1 Protein genetics, CpG Islands, DNA Methylation, Humans, Loss of Heterozygosity, Microsatellite Instability, MutL Protein Homolog 1, MutS Homolog 2 Protein genetics, Nuclear Proteins metabolism, Promoter Regions, Genetic, Adaptor Proteins, Signal Transducing genetics, Colorectal Neoplasms, Hereditary Nonpolyposis genetics, Epigenesis, Genetic, Germ-Line Mutation, Nuclear Proteins genetics
Abstract

Epimutations are errors in the normal process of epigenetic regulation which can result in aberrant transcriptional silencing of a normally active gene or reactivation of a normally silent gene. Epimutations are generally considered to be somatic events and to be confined in affected tissues. However, recent studies of patients with hereditary nonpolyposis colorectal cancer (HNPCC) have showed that allele-specific hypermethylation of CpG islands in the promoter region of the MLH1 gene, one of the causes of the tumor, existed in all the tissues examined. In addition, germ-line epimutations of other tumor suppressor genes (TSGs), such as MSH2 and BRCA1, have also been reported, demonstrating that epimutations might arise in the germ-line (during gametogenesis or early embryonic development). The role of germ-line epimutations might be as important as germ-line mutations in human disease. We reviewed the update on germ-line epimutations of TSGs including the possible mechanisms underlying germ-line epimutations, the possibility of transgenerational inheritance, and their impact on our understanding of human disease.

Published
2009
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