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We observed behavior response of overwintering Aythya baeri to different weather conditions by using fixed point-based observation and scanning sampling methods, at Henan Minquan National Wetland Park during November to December 2018. The results showed that, on sunny days, the dominant behaviors of A. baeri were resting, flying, and locomotion (65.5%), the second were foraging and maintaining (31.9%). The daily behavioral rhythm was foraging in the morning, resting at noon, and foraging and maintain in the afternoon. The flying usually occurred before the peak of foraging. The locomotion behavior was mostly accompanied by other behaviors, which positively associated with foraging and negatively correlated with resting. A. baeri increased resting and foraging in rainy days compared with that in sunny days. The dominant behaviors were resting, foraging and locomotion (76.5%), and maintaining and flying were the secondary (20.3%). The peaks of foraging and resting postponed to the evening, while the flying and maintaining were significantly decreased. Compared with the sunny days, the resting, foraging, locomotion and maintaining behaviors were increased in mist days, and flying was decreased. The peak of foraging delayed to the noon and afternoon, and that of resting postponed to the afternoon. The dominant behaviors were resting, locomotion and foraging (70.6%), while maintaining and flying behavior were the secondary (27.5%). In summary, there are variations in time allocation of A. baeri behaviors, activity rhythm and dominant behaviors due to the change of weather conditions during wintering. To overcome the bad weather conditions in rainy and mist days, A. baeri would allocate more time on foraging for increasing energy intake, and more resting time for reducing energy consumption. [ABSTRACT FROM AUTHOR]

Background: Despite the improved access to health services in China, inadequate diagnosis and management of dementia are common issues, especially in rural regions.Objective: The Hubei Memory & Aging Cohort Study was designed as a prospective study in Central China to determine the prevalence, incidence, and risk factors for dementia and mild cognitive impairment (MCI) among urban and rural older adults.Methods: From 2018-2020, participants aged ≥65 years were screened, and data regarding their life behaviors, families, socio-economic status, physical and mental health, social and psychological factors, and cognition were collected. Diagnoses of MCI and dementia were made via consensus diagnosis using the Diagnostic and Statistical Manual of Mental Disorders fourth edition criteria.Results: Of 8,221 individuals who completed their baseline clinical evaluation, 4,449 (54.1%) were women and 3,164 (38.4%) were from remote rural areas (average age: 71.96 years; mean education period: 7.58 years). At baseline, 25.98%(95%confidence interval [CI]: 24.99-26.96) and 7.24%(95%CI: 6.68-7.80) of the participants were diagnosed with MCI and dementia, respectively. Prevalence showed a strong relationship with age. The substantial disparities between rural and urban regions in MCI and dementia prevalence and multiple dementia-related risk factors were revealed. Especially for dementia, the prevalence rate in rural areas was 2.65 times higher than that in urban regions.Conclusion: Our results suggested that public health interventions are urgently needed to achieve equitable diagnosis and management for people living with dementia in the communities across urban and rural areas. [ABSTRACT FROM AUTHOR]

Bird species richness and diversity are the key factors that determine the health and productivity of an ecosystem. They have occupied a marvelous place, i.e., they play a vital role in the contribution of public consideration towards natural habitats and considered as barometers of an ecosystem. This study was carried out to determine the bird species diversity of the Sheikh Badin National Park, located in District Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan. For this purpose, bird watching surveys following line transect method were conducted from June 2018 to July 2019 in the study area. A total 3408 individuals (1260 migrants and 2148 residents) representing the 41 bird species were recorded during the surveys. Among the total, 18 species were migrants and 23 residents, belong to 11 Orders and 25 Families. Passeriformes was the dominant encompass of 19 species (9 native and 10 migrants) and 13 families. This national park harbored species richness was r = 41, Shannon-Weiner diversity index (H' = 3.546), evenness (J' 0.955) and Simpson's Diversity (D = 0.966). The foraging guild results revealed that the granivore (2274) and insectivore (565) were the most abundant bird species. The major threats to the avifauna were illegal hunting, grazing and deforestation. [ABSTRACT FROM AUTHOR]

A series of new Olaparib derivatives was designed and synthesized, and their inhibitory activities against poly (ADP-ribose) polymerases-1 (PARP-1) enzyme and cancer cell line MDA-MB-436 in vitro were evaluated. The results showed that compound 5l exhibited the most potent inhibitory effects on PARP-1 enzyme (16.10 ± 1.25 nM) and MDA-MB-436 cancer cell (11.62 ± 2.15 μM), which was close to that of Olaparib. As a PARP-1 inhibitor had been reported to be viable to neuroprotection, in order to search for new multitarget-directed ligands (MTDLs) for the treatment of Alzheimer's disease (AD), the inhibitory activities of the synthesized compounds against the enzymes AChE (from electric eel) and BChE (from equine serum) were also tested. Compound 5l displayed moderate BChE inhibitory activity (9.16 ± 0.91 μM) which was stronger than neostigmine (12.01 ± 0.45 μM) and exhibited selectivity for BChE over AChE to some degree. Molecular docking studies indicated that 5l could bind simultaneously to the catalytic active of PARP-1, but it could not interact well with huBChE. For pursuit of PARP-1 and BChE dual-targeted inhibitors against AD, small and flexible non-polar groups introduced to the compound seemed to be conducive to improving its inhibitory potency on huBChE, while keeping phthalazine-1-one moiety unchanged which was mainly responsible for PARP-1 inhibitory activity. Our research gave a clue to search for new agents based on AChE and PARP-1 dual-inhibited activities to treat Alzheimer's disease. [ABSTRACT FROM AUTHOR]

Lipid metabolic disorders, oxidative stress and inflammation in the liver are key steps in the progression of non-alcoholic fatty liver disease (NAFLD). Ophiopogonin D (OP-D), the main active ingredient of Ophiopogon japonicus, exhibits several pharmacological activities such as antioxidant and anti-inflammatory activities. Therefore, the current study aimed to explore the role of OP-D in NAFLD in a high-fat diet (HFD)-induced obesity mouse model. To investigate the effect of OP-D on NAFLD in vivo, a NAFLD mouse model was established following feeding mice with HFD, then the mice were randomly treated with HFD or HFD + OP-D for 4 weeks. Subsequently, primary mouse hepatocytes were isolated, and enzyme-linked immunosorbent assay, reverse transcription-quantitative PCR western blotting and immunofluorescence analysis were used for assessment to explore the direct effect of OP-D in vitro. The results of the present study indicated that OP-D could ameliorate NAFLD in HFD-induced obese mice by regulating lipid metabolism and antioxidant and anti-inflammatory responses. Additionally, OP-D treatment decreased lipogenesis and inflammation levels in vitro, suggesting that the NF-κB signaling pathway may be involved in the beneficial effects of OP-D on NAFLD. [ABSTRACT FROM AUTHOR]

By using the methods of fixed-spot observation, GPS positioning, sampling plot investigation, and factor analysis, the forage habitat selection of white-naped crane (Grus vipio) during its incubation period in Zhalong Wetland was studied from April to May 2002-2008. In the study area, the crane during its incubation period chose a variety of habitat types as its forage habitat, among which, wetland (reed swamp) was the main one, occupying 74. 36% of the total, followed by farmland. The forage habitat included four elements, i. e., habitat identification, food, water, and cover, and the main selection criteria were the identification factor and energy factor, followed by eating feasibility factor, security distance factor, and concealment factor. The forage habitat selection of the crane during its incubation period had two levels (macro- and microhabitat) and three courses (forage habitat type choice, forage area choice, and forage microhabitat choice). [ABSTRACT FROM AUTHOR]

We observed behavior response of overwintering Aythya baeri to different weather conditions by using fixed point-based observation and scanning sampling methods, at Henan Minquan National Wetland Park during November to December 2018. The results showed that, on sunny days, the dominant behaviors of A. baeri were resting, flying, and locomotion (65.5%), the second were foraging and maintaining (31.9%). The daily behavioral rhythm was foraging in the morning, resting at noon, and foraging and maintain in the afternoon. The flying usually occurred before the peak of foraging. The locomotion behavior was mostly accompanied by other behaviors, which positively associated with foraging and negatively correlated with resting. A. baeri increased resting and foraging in rainy days compared with that in sunny days. The dominant behaviors were resting, foraging and locomotion (76.5%), and maintaining and flying were the secondary (20.3%). The peaks of foraging and resting postponed to the evening, while the flying and maintaining were significantly decreased. Compared with the sunny days, the resting, foraging, locomotion and maintaining behaviors were increased in mist days, and flying was decreased. The peak of foraging delayed to the noon and afternoon, and that of resting postponed to the afternoon. The dominant behaviors were resting, locomotion and foraging (70.6%), while maintaining and flying behavior were the secondary (27.5%). In summary, there are variations in time allocation of A. baeri behaviors, activity rhythm and dominant behaviors due to the change of weather conditions during wintering. To overcome the bad weather conditions in rainy and mist days, A. baeri would allocate more time on foraging for increasing energy intake, and more resting time for reducing energy consumption.

Fragile X syndrome (FXS) is the most common inherited form of intellectual disability, resulted from the silencing of the Fmr1 gene and the subsequent loss of fragile X mental retardation protein (FMRP). Spine dysgenesis and cognitive impairment have been extensively characterized in FXS; however, the underlying mechanism remains poorly understood. As an important regulator of spine maturation, intercellular adhesion molecule 5 (ICAM5) mRNA may be one of the targets of FMRP and involved in cognitive impairment in FXS. Here we show that in Fmr1 KO male mice, ICAM5 was excessively expressed during the late developmental stage, and its expression was negatively correlated with the expression of FMRP and positively related with the morphological abnormalities of dendritic spines. While in vitro reduction of ICAM5 normalized dendritic spine abnormalities in Fmr1 KO neurons, and in vivo knockdown of ICAM5 in the dentate gyrus rescued the impaired spatial and fear memory and anxiety-like behaviors in Fmr1 KO mice, through both granule cell and mossy cell with a relative rate of 1.32 ± 0.15. Furthermore, biochemical analyses showed direct binding of FMRP with ICAM5 mRNA, to the coding sequence of ICAM5 mRNA. Together, our study suggests that ICAM5 is one of the targets of FMRP and is implicated in the molecular pathogenesis of FXS. ICAM5 could be a therapeutic target for treating cognitive impairment in FXS. SIGNIFICANCE STATEMENT Fragile X syndrome (FXS) is characterized by dendritic spine dysgenesis and cognitive dysfunctions, while one of the FMRP latent targets, ICAM5, is well established for contributing both spine maturation and learning performance. In this study, we examined the potential link between ICAM5 mRNA and FMRP in FXS, and further investigated the molecular details and pathological consequences of ICAM5 overexpression. Our results indicate a critical role of ICAM5 in spine maturation and cognitive impairment in FXS and suggest that ICAM5 is a potential molecular target for the development of medication against FXS., (Copyright © 2020 Pei et al.)

Long noncoding RNAs (lncRNAs) are emerging as important regulators during tumorigenesis by serving as competing endogenous RNAs (ceRNAs). In this study, the qRT-PCR results indicated that the lncRNA protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P) was overexpressed in oral squamous cell carcinoma (OSCC) and decreased the survival rate of OSCC patients. CCK-8 and clonal colony formation assays were used to detect the effects of PDIA3P on proliferation. Results revealed that silencing PDIA3P by small interfering RNA (siRNA) inhibited OSCC cell proliferation and repressed tumor growth and reduced the expression of proliferation antigen Ki-67 in vivo. Furthermore, the interaction between PDIA3P and miRNAs was then analyzed by qRT-PCR and luciferase reporter gene assay. We found that PDIA3P negatively regulated miR-185-5p in OSCC cells. Simultaneously, we found that silencing PDIA3P by siRNA suppressed proliferation via miR-185-5p in OSCC cells. Moreover, silencing PDIA3P by siRNA inhibited CCND2 protein (no influence on mRNA levels) expression via miR-185-5p in OSCC cells, and CCND2 facilitated cell proliferation of SCC4 and SCC15 cells induced by sh-PDIA3P#1. Therefore, our study demonstrated that PDIA3P may be a therapeutic target for the treatment of OSCC., (Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.)