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Using laser to remove foreign matters on overhead high-voltage transmission lines has already become a mainstream method in recent years. In this paper, by analyzing the procedures of the removing operation as well as its demand on the control system of the remover device, a novel design of control system is proposed, in which the calculation are mainly performed in a generic smart terminal device connecting wirelessly to the actuator-driving part. The complete hardware design and software flow are also introduced. Comparing to the conventional integrated control system, this design is more flexible, easier to maintain and upgrade, and is of lower cost. Experiments show that, remover device controlled by this system can perform the removing operation accurately and reliably.

Primary cilia are enriched in signaling receptors, and defects in their formation or function can induce conditions such as polycystic kidney disease, postaxial hexadactyly, and microphthalmia. Mammalian Hedgehog (Hh) signaling is important in the development of primary cilia, and TMEM216, a transmembrane protein that localizes to the base of cilia, is also implicated in ciliogenesis in zebrafish. Here, we found that Tmem216-deficient mice had impaired Hh signaling and displayed typical ciliopathic phenotypes. These phenomena were also observed in cells deficient in TMEM216. Furthermore, TMEM216 interacted with core Hh signaling proteins, including SUFU, a negative regulator of Hh, and GLI2/GLI3, transcription factors downstream of Hh. The competition between TMEM216 and SUFU for binding to GLI2/GLI3 inhibited the cleavage of GLI2/GLI3 into their repressor forms, which resulted in the nuclear accumulation of full-length GLI2 and the decreased nuclear localization of cleaved GLI3, ultimately leading to the activation of Hh signaling. Together, these data suggest that the TMEM216-SUFU-GLI2/GLI3 axis plays a role in TMEM216 deficiency–induced ciliopathies and Hh signaling abnormalities. Editor's summary: The normal development of primary cilia is necessary for signal transduction, particularly Hedgehog (Hh) signaling, and impaired ciliogenesis underlies various developmental disorders. Noting that the transmembrane protein TMEM216 is implicated in ciliogenesis in zebrafish, Wang et al. found that Tmem216−/− mice had impaired ciliogenesis and reduced Hh signaling. TMEM216 competed with a negative regulator of Hh signaling, SUFU, for binding to GLI transcription factors, inhibiting the cleavage of GLI2/GLI3 into their repressor forms, thus promoting Hh signaling. Together, these findings delineate how TMEM216 promotes Hh signaling during ciliogenesis. —Amy E. Baek [ABSTRACT FROM AUTHOR]

A novel multifunctional nanoprobe was designed for cancer cell targeted multilayer imaging of two cancer biomarkers. Based on the proposed method, in situ imaging of membrane MUC1 mucin and cytoplasmic microRNA miR-21 coupled with precise photodynamic therapy was achieved. [ABSTRACT FROM AUTHOR]