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38 results on '"Wu., Yinyuan"'

11. Caspase 3 is Activated through Caspase 8 instead of Caspase 9 during H(2)O(2)-induced Apoptosis in HeLa Cells

Author

Wu, Yinyuan, Wang, Dianjun, Wang, Xiaodong, Wang, Yinyin, Ren, Fangli, Chang, Donald C, Chang, Zhijie, Jia, Baoqing, Wu, Yinyuan, Wang, Dianjun, Wang, Xiaodong, Wang, Yinyin, Ren, Fangli, Chang, Donald C, Chang, Zhijie, and Jia, Baoqing

Abstract

Oxidative stress is known to be involved in a variety of pathological processes including atherosclerosis, diabetes, and neurodegenerative diseases. Understanding how intracellular signaling pathways respond to oxidative stress will have a significant implication in the therapy of these diseases. In this study, we applied hydrogen peroxide (H(2)O(2)) to trigger apoptosis and investigated the dynamic activation of various caspases using a FRET technique. We measured the activation dynamics of caspase 3 and caspase 9 based on two reporter systems, SCAT 3 and SCAT 9. We found that caspase 3 activation was earlier than that of caspase 9 following H(2)O(2) treatment. Caspase 3 was activated rapidly, reaching a maximum in 12 +/- 3 min, while the average duration of caspase 9 activation was 21 +/- 3 min. When cells were pretreated with Z-LEHD-fmk, a caspase 9 specific inhibitor, caspase 3 activation and apoptosis by H(2)O(2) treatment were little affected, although the caspase 9 activation was completely inhibited. When cells were pretreated with Z-DEVD-fmk, a caspase 3 specific inhibitor, the activation of both caspase 3 and caspase 9, as well as apoptosis, were inhibited. When cells were pretreated with Z-IETD-fmk, a caspase 8 specific inhibitor, the activation of caspase 3 and caspase 9 were significantly delayed. Finally, we found that Bax did not translocate from the cytosol to the mitochondrial membrane during H(2)O(2)-induced apoptosis. Our results suggest that, during H(2)O(2)-induced apoptosis, caspase 3 is activated directly through caspase 8 and is not through the mitochondria-dependent caspase 9 activation. Copyright (C) 2011 S. Karger AG, Basel

Published
2011

13. GABARAPL1 Negatively Regulates Wnt/β-catenin Signaling by Mediating Dvl2 Degradation through the Autophagy Pathway

Author

Zhang, Yanquan, primary, Wang, Fang, additional, Han, Liang, additional, Wu, Yinyuan, additional, Li, Shan, additional, Yang, Xi, additional, Wang, Yinyin, additional, Ren, Fangli, additional, Zhai, Yonggong, additional, Wang, Dianjun, additional, Jia, Baoqing, additional, Xia, Yongjing, additional, and Chang, Zhijie, additional

Published
2011
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17. Caspase 3 is Activated through Caspase 8 instead of Caspase 9 during H2O2-induced Apoptosis in HeLa Cells.

Author

Wu, Yinyuan, Wang, Dianjun, Wang, Xiaodong, Wang, Yinyin, Ren, Fangli, Chang, Donald, Chang, Zhijie, and Jia, Baoqing

Subjects
*PROTEOLYTIC enzymes, *APOPTOSIS, *CYTOLOGY, *HYDROGEN peroxide, *OXIDATIVE stress, *ATHEROSCLEROSIS, *MITOCHONDRIAL membranes
Abstract

Oxidative stress is known to be involved in a variety of pathological processes including atherosclerosis, diabetes, and neurodegenerative diseases. Understanding how intracellular signaling pathways respond to oxidative stress will have a significant implication in the therapy of these diseases. In this study, we applied hydrogen peroxide (H2O2) to trigger apoptosis and investigated the dynamic activation of various caspases using a FRET technique. We measured the activation dynamics of caspase 3 and caspase 9 based on two reporter systems, SCAT 3 and SCAT 9. We found that caspase 3 activation was earlier than that of caspase 9 following H2O2 treatment. Caspase 3 was activated rapidly, reaching a maximum in 12±3 min, while the average duration of caspase 9 activation was 21±3 min. When cells were pretreated with Z-LEHD-fmk, a caspase 9 specific inhibitor, caspase 3 activation and apoptosis by H2O2 treatment were little affected, although the caspase 9 activation was completely inhibited. When cells were pretreated with Z-DEVD-fmk, a caspase 3 specific inhibitor, the activation of both caspase 3 and caspase 9, as well as apoptosis, were inhibited. When cells were pretreated with Z-IETD-fmk, a caspase 8 specific inhibitor, the activation of caspase 3 and caspase 9 were significantly delayed. Finally, we found that Bax did not translocate from the cytosol to the mitochondrial membrane during H2O2-induced apoptosis. Our results suggest that, during H H2O2-induced apoptosis, caspase 3 is activated directly through caspase 8 and is not through the mitochondria-dependent caspase 9 activation. Copyright © 2011 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2011
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18. Rapid Extraction of Lexical Tone Phonology in Chinese Characters: A Visual Mismatch Negativity Study

Author

Wang, Xiao-Dong, Liu, A-Ping, Wu, Yinyuan, and Wang, Peng

Subjects
Biology, Neuroscience, Cognitive Neuroscience, Cognition, Sensory Perception, Psychoacoustics, Psychophysics, Behavioral Neuroscience, Neurolinguistics, Neuropsychology, Social and Behavioral Sciences, Linguistics, Phonology, Psycholinguistics
Abstract

Background: In alphabetic languages, emerging evidence from behavioral and neuroimaging studies shows the rapid and automatic activation of phonological information in visual word recognition. In the mapping from orthography to phonology, unlike most alphabetic languages in which there is a natural correspondence between the visual and phonological forms, in logographic Chinese, the mapping between visual and phonological forms is rather arbitrary and depends on learning and experience. The issue of whether the phonological information is rapidly and automatically extracted in Chinese characters by the brain has not yet been thoroughly addressed. Methodology/Principal Findings We continuously presented Chinese characters differing in orthography and meaning to adult native Mandarin Chinese speakers to construct a constant varying visual stream. In the stream, most stimuli were homophones of Chinese characters: The phonological features embedded in these visual characters were the same, including consonants, vowels and the lexical tone. Occasionally, the rule of phonology was randomly violated by characters whose phonological features differed in the lexical tone. Conclusions/Significance: We showed that the violation of the lexical tone phonology evoked an early, robust visual response, as revealed by whole-head electrical recordings of the visual mismatch negativity (vMMN), indicating the rapid extraction of phonological information embedded in Chinese characters. Source analysis revealed that the vMMN was involved in neural activations of the visual cortex, suggesting that the visual sensory memory is sensitive to phonological information embedded in visual words at an early processing stage.

Published
2013
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27. Outcomes of Induction Chemotherapy for Head and Neck Cancer Patients

Author

Chen, Jin-Hua, Yen, Yu-Chun, Liu, Shing-Hwa, Yuan, Sheng-Po, Wu, Li-Li, Lee, Fei-Peng, Lin, Kuan-Chou, Lai, Ming-Tang, Wu, Chia-Che, Chen, Tsung-Ming, Chang, Chia-Lun, Chow, Jyh-Ming, Ding, Yi-Fang, Lin, Ming-Chin, Wu, Szu-Yuan, and Wu., Yinyuan

Abstract

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Published
2016
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