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Dyslipidemia is an important risk factor of atherosclerotic cardiovascular disease (ASCVD). Statins delay the occurrence and development of ASCVD, and reduce the risk of cardiovascular events and death. Due to safety concerns, there exist insufficient use of lipid-lowering agents and a high withdrawal rate of the agents in the elderly. To promote the prevention and treatment of ASCVD, this expert consensus is issued and focuses on the management of dyslipidemia of Chinese elderly basing on the clinical evidence of the use of lipid-lowering drugs by the elderly, and the lipid management guidelines and expert consensus recommendations at home and abroad.血脂异常是导致动脉粥样硬化性心血管疾病（ASCVD）的重要危险因素，他汀类药物延缓ASCVD的发生、发展并降低心血管事件及死亡的风险。由于对安全性的担忧，老年人调脂药物使用不足、停药率高。本专家共识依据老年人使用调脂药物的临床证据，参考国内外血脂管理指南及专家共识推荐，对我国老年人的血脂异常提出管理建议，旨在促进我国老年ASCVD防治工作。.

伴有破骨样巨细胞的胰腺未分化癌（UCPOGC）是胰腺导管腺癌的一种罕见的特殊亚型。肿瘤成分复杂，其中可见3种细胞类型：肿瘤细胞、单核组织细胞和破骨样巨细胞。肿瘤细胞异型性显著，通常不表达或仅灶性表达上皮标志物，而弥漫表达间叶源性标志物波形蛋白，因此常被误诊为肉瘤；后两者表达组织细胞标志物，缺乏上皮分化。现报道2例UCPOGC，分析临床病理学特征，并查阅相关文献，重点讨论其组织起源及预后相关因素，以提高临床与病理医师对该肿瘤的认识水平。.

With the development of high-rise buildings, wide and deep foundations have been extensively applied to practical projects during the last several decades. This research aims to develop a solution that applies to wide and deep foundations with the necessary accuracy. Considering the influences of matric suction, adjusted soil parameters, wall friction, and stress strength nonlinearities from high matric suction, a solution for estimating the bearing capacity of strip foundations on unsaturated soils is presented based on the unified strength theory of unsaturated soils and the upper bound method of limit analysis. The validity and applicability of the solution are verified compared with Meyerhof's solution and Zhang's solution. The influences of the breadth, depth/breadth ratio, wall friction, matric suction, and adjustment factor of soil parameters upon the bearing capacity are discussed. Furthermore, a simplified chart-lookup method is presented for practical application. Because it includes the wall friction and assumes only a single failure pattern in derivation, the solution developed for the bearing capacity of strip foundations applies to both shallow and deep foundations.

This paper proposes an Advanced Encryption Standard (AES) encryption technique based on memristive neural network. A memristive chaotic neural network is constructed by the use of the nonlinear characteristics of the memristor. The chaotic sequence, which is sensitive to the initial value and has good random characteristics, is used as the initial key of AES grouping to realize "one-time-one-secret" dynamic encryption. Results show that the algorithm has higher security, larger key space and stronger robustness than the conventional AES. It can effectively resist the initial key fixed and exhaustive attacks.

Background Knowledge of Acute Myocardial Infarction (AMI) symptoms and risk factors (RF) have been studied worldwide in various populations. However, the studied specific symptom and RF of different surveys varies, the number of surveyed symptom and RF varies, and the criteria for judging good/high knowledge and bad/low knowledge are different (group classification criteria include the number, the percentages or the scores), which makes the results difficult to compare. Purpose The aim was to identify clusters of knowledge on symptoms and modifiable RFs using Latent Cluster Analysis, and to further compare characteristics and the awareness of the need for prompt treatment among different clusters. Methods A structured questionnaire was used to interview 4122 community residents over 35 years old on their knowledge about AMI symptoms and RFs. Latent GOLD Statistical Package was used to identify and classify the respondent subtypes (latent classes) of the knowledge on AMI symptoms or modifiable RFs. An individual's class membership probabilities are computed from the estimated model parameters and the observed scores. The multivariable logistic regression was performed in order to identify factors that predicted High knowledge membership. Results Two and three distinct clusters were identified in terms of knowledge of symptoms and RFs. 62.7% (Symptom High Knowledge Cluster) and 39.5% (RF High Knowledge Cluster) of the respondents were able to identify most symptoms and modifiable RFs. A number of common differences were observed with respect to higher probability of having high knowledge on symptom and RF, which was mostly better among those with higher education and average monthly income, insured, having annual physical examination, have disease history of AMI RFs, AMI history in immediate family member or acquaintance and have been exposed to AMI related public education. There was also significant difference in awareness of the prompt treatment in case of AMI occurs among different symptom or RF clusters. Conclusions A moderate or relatively low knowledge regarding AMI symptoms and modifiable RFs was observed in our study. Identification of Symptom or RF knowledge clusters can be a way to detect specific targeted groups that are most likely to possess lower knowledge of AMI with readily identifiable characteristics, which may facilitate health education and further reduce the pre-hospital delay and improve patient outcomes. Funding Acknowledgement Type of funding sources: Foundation. Main funding source(s): Key Project of Scientific and Technological Support Plan of Tianjin in 2020 Cluster category chartProfile plot

Background Patient delay in seeking help has been reported to be a major factor that related to delay in care of acute myocardial infarction (AMI). Lack the knowledge of symptoms may translate to behavioral deficiencies which lead up to failing to seek medical assistant at early stage of AMI. Purpose The aim of present study is to investigate the awareness of AMI symptoms and the emergency responses among community residents over 35 years old. Methods The sample was proportionally distributed by age according to the national census data. The sex ratio was 1:1. The sample size is calculated according to the significance level of 0.05, the allowable error of 0.1, and the estimated Excellent awareness level of 10%. The final sample size is adjusted to 4200, considering non-response rate of 20%. Multi-stage stratified random sampling was used. On the first stage, two districts each in urban and rural regions were randomly selected. On the second stage, 3–10 community health service centers were randomly selected in each district. On the third stage, residents over 35 managed by the community health service center were proportionally sampled to be interviewed. A structured questionnaire was used and the survey was conducted in a face-to-face interview by clinical physicians. Logistic regression was applied to analyze factors related to Good knowledge. Results The top three symptom recognized by public is “pain or discomfort in the chest” (71.3%), followed by “difficulty breathing” (65.1%) and “pain or discomfort in the jaw, neck, or back” (60.9%). 85.0% chose to call an ambulance as first response when witness others having an AMI. Those who lives alone (OR=1.408; 95% CI, 1.005–1.972) and whose immediate family had been diagnosed with AMI (OR=1.510; 95% CI, 1.040–2.192) has better knowledge. A significant positive correlation was observed for those with hypertension (OR=1.199; 95% CI, 1.007–1.429), while a negative correlation was observed for those with diabetes (OR=0.788; 95% CI, 0.626–0.992). Public education could improve Good knowledge (OR=1.662; 95% CI, 1.388–1.990), while doctoral advise has been shown negative impact (OR=0.824; 95% CI, 0.691–0.984). Conclusions Our data provide first population-based estimates of public awareness in our country. Further promotional strategies to increase overall awareness in general public are seriously needed. Funding Acknowledgement Type of funding sources: Foundation. Main funding source(s): Major Science and Technology Projects of Tianjin Science and Technology Commission Multivariable logistic regression

Background The role of proprotein convertase subtilisin/kexin type 9 (PCSK9) in predicting major adverse cardiovascular events (MACEs) in Non-ST elevation myocardial infarction (NSTEMI) patients is still an open question and the PCSK9 concentration of clinical usefulness remains unknown in guiding treatment. Purpose To explore the role of PCSK9 in predicting major adverse cardiovascular events (MACEs) in Non-ST elevation myocardial infarction patients. Methods 272 patients with NSTEMI were included in our study, all patients received PCI therapy after admission. Patients were followed up for 1 year and MACEs were recored. Their baseline plasma PCSK9 levels were determined by ELISA. Patients were divided into high, medium and low PCSK9 groups and the associations of PCSK9 with other biomarkers and MACEs were evaluated. Results The results showed that PCSK9 levels was related to levels of lipoproteins, high-sensitivity C-reactive protein (r=0.162, P=0.008), platelet volume distribution width (r=0.299, P Conclusions In a NSTEMI setting, the concentration of PCSK9 is associated with hypercoagulability and hyper-inflammation. High levels of PCSK9 independently predict future MACEs in NSTEMI patients, particularly those classified by the GRACE score as high risk. Funding Acknowledgement Type of funding sources: None.

Steel chimney is a kind of high-rise structure, which is usually built in areas with large wind loads. And the possibility of large deformation or even damage caused by wind vibration exists. Suspended tuned mass damper (STMD) has been proved to be an effective vibration control device, which can effectively reduce the dynamic response of high-rise structures caused by wind and ensure it meets the requirements of deformation and comfort. A STMD is designed in a seaside thermodynamic boiler chimney to reduce the wind-induced response of the structure. The AR method for simulating the time-course load of along-wind is firstly introduced, with Davenport wind speed spectrum used in fitting. It can be concluded that the fluctuating wind obtained by the AR method meets the requirements in statistical sense. Then the simulation of cross-wind is proposed according to the range of Reynolds number, and the coupling of along-wind and cross-wind is taken into consider. The effect of STMD on vibration reduction is proved by analysis and test on the spot by comparing the acceleration amplitude of the top of the chimney with or without the STMD.

Traumatic brain injury (TBI) induced neuroinflammation is featured as excessive glial inflammatory activation and violent neurologic destruction and dysfunction. Massive microglia activation in situ and disrupt of blood-brain barrier contribute to severely collapsed nervous system. Tizoxanide (TIZ), a synthetic thiazolide derivative agent possessing a broad-spectrum anti-infective effect, currently shows a potential resistance against pathogens like bacteria, virus and parasites, while its underlying role in neuroinflammation is elusive. The study aimed to explore the effect of TIZ on neuroinflammation in vitro microglia.Primary microglia were accepted to neuroinflammatory activation via lipopolysaccharide (LPS) administration. TIZ was conducted to pretreatment of microglia. Cell viability, inflammatory cytokines, chemotaxis, nitric oxide release, inflammation-related enzymes, and mitogen-activated protein kinase (MAPK) pathway activation in microglia were investigated respectively.We demonstrated that TIZ administration attenuates inflammatory cytokines and chemokines through quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) of medium supernatant. In addition, TIZ reduces pro-inflammatory mediators and nitric oxide release in microglia. Furtherly, TIZ inhibits the level of p38/MAPK pathway in LPS stimuli, indicating that TIZ negatively regulates neuroinflammation via inhibiting p38/MAPK pathway.TIZ is verified to be an anti-inflammation effect on neuroinflammation in microglia via downregulation of p38/MAPK pathway, which restrains inflammation by reduced inflammatory cytokines, chemokines and mediators and decreased nitric oxide release. To summarize, TIZ is considered to be a promising reagent to alleviate neuroinflammation targeting microglia in nervous system injury.

MiR-222-3р has been implicated in tumor cell proliferation and has an important role in the differentiation and maturation of myogenic cells. However, its role in skeletal myoblast proliferation is still unclear. In this study, we found that miR-222-3р expression increases initially and then decreases during C2C12 myoblast proliferation. Using synthetic miRNA mimics and inhibitors in gain- or loss-of-function experiments, we snowed that miR-222-3р overexpression in C2C12 cells promotes myoblast proliferation and represses myofiber formation, while miR-222-3р downregulation has the opposite effect. Using a prediction program, BTG2 was identified as a possible target gene of miR-222-3р. During myogenesis, miR-222-3р mimics repress BTG2 expression, while miR-222-3р inhibitors promote BTG2 expression. Using dual-luciferase reporter assay, we further demonstrated that miR-222-3р specifically targets BTG2. Additionally, we show that siRNA-mediated downregulation of BTG2 expression in C2C12 myoblasts promotes the proliferation and suppresses differentiation. In conclusion, we provide a novel insight into the mechanism by which miR-222-3р regulates the proliferation and differentiation of C2C12 myoblasts by targeting BTG2. This information contributes to our understanding of the role of miRNAs in skeletal muscle development.

Contrast agents (CAs) play an important role in enhancing the magnetic resonance imaging (MRI) performance for accurate tumor diagnosis, which, however, may give rise to unexceptional issues such as in vivo accumulation and bio-toxicity. Here we report on manganese silicate nanoparticles (denoted as MnSNs) as highly biocompatible and pH/GSH-responsive T

Considering the pressure of peak shaving, difficulty of local absorption and high rate of wind abandonment caused by large-scale new energy absorption, this paper proposes a new energy absorption risk early warning method based on differential index weight. The purpose is to solve the problems caused by large-scale new energy access from the aspects of per-planning, construction and access system of new energy projects. The principles of risk early warning system construction for new energy planning and construction are put forward, and the risk early warning index system is constructed based on the risk of absorptive capacity, networking conditions and project completion rate. The risk early warning mechanism is established, and the weight coefficients of each index are differentiated by using the analytic hierarchy process. A risk early warning evaluation model suitable for different power grid environments is obtained. Taking a power grid as an example, the proposed model and method are verified. The results show that the proposed method is accurate in evaluating the new energy absorption capacity of 14 cities, and has validity, practicability and application value.

We experimentally investigate the energy-resolved angular distributions (ADs) of low-energy photoelectrons from above-threshold ionization (ATI) of Xe in intense infrared laser fields. An unexpected wavelength dependence of ADs for the low-energy structure (LES), in striking contrast to the prediction of the Coulomb-corrected semiclassical model, has been found. By improving the semiclassical model with non-adiabatic corrections, the experimental features can be qualitatively reproduced, and we suggest the indispensable role of the non-adiabatic effect in producing the LES. Our finding provides new insights into the understanding of low-energy electron dynamics of atoms with intense laser fields.

Adipogenesis plays a key role in increasing fat mass, which is a main characteristic for obesity, and involves preadipocyte proliferation and differentiation. Recently, more and more evidences suggested that microRNAs (miRNAs) is an important member of the regulatory network of adipogenesis. In this study, miR-143a-3p was highly expressed in adipose tissues of obese mice, and was up-regulated at the middle and last stage of 3T3-L1 adipocyte differentiation. Using mouse 3T3-L1 cells line, which is an ideal model in vitro for the study of adipogenesis, we observed that overexpression of miR-143a-3p inhibited the preadipocyte proliferation, and enhanced the preadipocyte differentiation. In contrast, the inhibition of miR-143a-3p expression promoted the preadipocyte proliferation, and inhibited the preadipocyte differentiation. Further analysis suggested that miR-143a-3p mediating preadipocyte differentiation might be involved in fatty acid metabolism. In addition, we found that miR-143-3p and PPARγ, an activator of miR-143a-3p transcription, could regulate each other. Compared with miR-143a-3p, MAPK7 played an opposite role in the proliferation and differentiation of adipocyte. Further analysis indicated that MAPK7 is a target gene of miR-143a-3p in 3T3-L1 cells, and inhibition of MAPK7 recede the effect of miR-143a-3p on preadipocyte proliferation and differentiation. Taken together, these results indicated that as a regulator of PPARγ, miR-143a-3p play an important role in adipogenesis via regulating MAPK7 and fatty acid.

Background The Liangshan breed of pig is well known for its excellent meat quality attributes, but poor carcass quality and growth attributes have limited its economic value. By crossing the Liangshan with western breeds that have strong growth attributes may improve the general performance of the breed. Objectives The objective of this research was to study the effects of slaughter age and cross-breeding on reproduction, growth, carcass, meat quality, and to estimate the heterosis values of the various traits. Materials Samples were taken from 636 pigs of three different genotypes [Liangshan pure breed (LS), Duroc-Liangshan cross (DL), and Berkshire-Liangshan cross (BL)]. Results DL and BL F1 generations showed improved reproductive and growth traits than purebred Liangshan pigs. DL and BL had better carcass quality than LS, with a significant reduction in backfat thickness and sebum rate. Crossbreeds had higher L24 h and shear force, and lower marbling scores than LS. Drip loss in DL was markedly lower than in LS and BL, but BL had the lowest cooking loss, higher L45 min and pH45 min. By estimating heterosis, reproduction traits were significantly improved in both DL and BL. Growth performance showed no significant difference among the breeds. Backfat thickness and loin muscle area was significantly improved in the DL and BL breeds, although there was no difference in lean percentage and sebum rate. BL showed improved heterosis, with excellent intramuscular fat, higher pH45 min, lower L45 min and cooking loss. Conclusions The results of this study suggest that crossing Berkshire with Liangshan pigs could be an alternative genotype in the commercial pork production system.

The precipitation behavior of the second phase in Fe-18Cr-17Mn-2Mo-0.85N high nitrogen and nickel-free austenitic stainless steel after grain boundary character distribution (GBCD) optimization was investigated. The results show that the fraction of low Σ coincidence site lattice (CSL) boundaries increases from 47.9% for the solid solution treated specimen to 82.25% for the specimen cold-rolled by 5% and then annealed at 1423 K for 72 h (r5%-a1423 K/72 h). Compared with the solution treated sample, nitride precipitation is obviously restrained and the fraction of precipitates is the least in r5%-a1423 K/72 h sample with the highest proportion of SBs. The appearance of a high proportion of CSL boundaries with low energy inhibits the nitrides precipitation through GBCD optimization.

Green leaf volatiles (GLVs) have been reported to play an important role in the host-locating behavior of several folivores that feed on angiosperms. However, next to nothing is known about how the green leafhopper, Empoasca vitis, chooses suitable host plants and whether it detects differing emission levels of GLV components among genetically different tea varieties. Here we found that the constitutive transcript level of the tea hydroperoxide lyase (HPL) gene CsiHPL1, and the amounts of (Z)-3-hexenyl acetate and of total GLV components are significantly higher in tea varieties that are susceptible to E. vitis (Enbiao (EB) and Banzhuyuan (BZY)) than in varieties that are resistant to E. vitis (Changxingzisun (CX) and Juyan (JY)). Moreover, the results of a Y-tube olfactometer bioassay and an oviposition preference assay suggest that (Z)-3-hexenyl acetate and (Z)-3-hexenol offer host and oviposition cues for E. vitis female adults. Taken together, the two GLV components, (Z)-3-hexenol and especially (Z)-3-hexenyl acetate, provide a plausible mechanism by which tea green leafhoppers distinguish among resistant and susceptible varieties. Future research should be carried out to obtain the threshold of the above indices and then assess their reasonableness. The development of practical detection indices would greatly improve our ability to screen and develop tea varieties that are resistant to E. vitis.

The Jining Group occurs as the eastern segment of the Khondalite Belt, North China Craton and is dominated by a series of granulite facies rocks involving ‘normal’ pelitic granulites recording peak temperatures of ~850 °C and ultrahigh-temperature (UHT) pelitic granulites recording peak temperatures of 950–1100 °C. The P–T paths and ages of these two types of granulites are controversial. Three pelitic granulite samples in the Jining Group comprising two sillimanite–garnet gneiss samples (J1208 and J1210) and one spinel–garnet gneiss sample (J1303) were collected from Zhaojiayao, where ‘normal’ pelitic granulites occur, for determination of their metamorphic evolution and ages. Samples J1208 and J1210 are interpreted to record cooling paths from the Tmax stages with P–T conditions respectively of ~870–890 °C/7–8 kbar and >840 °C/>7.5 kbar constrained from the stability fields of the observed mineral assemblages and the isopleths of plagioclase, garnet and biotite compositions in pseudosections. Sample J1303 is interpreted to record pre-Tmax decompression from the kyanite-stability fields to the Tmax stage of 950–1020 °C/8–9 kbar and a post-Tmax cooling path revealed mainly from the stability field of the observed mineral assemblage, the plagioclase zoning and the biotite composition isopleth in pseudosections. The post-Tmax cooling stage can be divided into suprasolidus and subsolidus stages. The suprasolidus cooling may not result in an equilibrium state at the solidus in a rock. Therefore, different minerals may record different P–T conditions along the cooling path; the inferred maximum temperature is commonly higher than the solidus as well as different solidi being recorded for different samples from the same outcrop but experiencing different degrees of melt loss. Plagioclase compositions, especially its zoning in plagioclase-rich granulites, are predicted to be useful for recording the higher temperature conditions of a granulite's thermal history. The three samples studied seem to record the temperature range covering those of the ‘normal’ and UHT pelitic granulites in the Jining Group, suggesting that UHT conditions may be reached in ‘normal’ granulites without diagnostic UHT indicators. LA-ICP-MS zircon U–Pb data provide a continuous trend of concordant 207Pb/206Pb ages from 1.89 to 1.79 Ga for sample J1210, and from 1.94 to 1.80 Ga for sample J1303. These continuous and long age spectrums are interpreted to represent a slow cooling and exhumation process corresponding to the post-Tmax cooling P–T paths recorded by the pelitic granulites, which may have followed the exhumation of deeply buried rocks in a thickened crust region resulted from a collision event at c. 1.95 Ga as suggested by the pre-Tmax decompression P–T path.

Peach (Prunus persica [L.] Batsch; Rosaceae) is an economically important deciduous fruit. China is the largest producer of peaches, providing >50% of world production. Fruit rot is a devastating problem both pre- and postharvest. In May 2019, 882 infected peach fruits covered with white fluffy masses and brown necrotic lesions were observed among 4,500 fruits investigated in a commercial orchard in Jinshan, Shanghai, China. Disease incidence was ∼20%. Infected fruits were collected and kept in separate paper bags. Small sections (5 × 5 mm) were excised from the boundary of necrotic and healthy tissues, surface sterilized with 70% ethanol for 30 s and 5% NaOCl for 90 s, rinsed twice with sterilized distilled water (DW), and aseptically placed onto PDA. After 2 days of incubation at 26°C, sections from new colonies were transferred onto fresh PDA and purified by the single-spore method (Yang et al. 2008). Colonies on PDA had white aerial mycelia at first that turned off-white to light yellow after 5 days. No microconidia were observed. Seven morphologically similar isolates were obtained and representative isolate FQ01 analyzed. Macroconidia were five to seven septate with a tapered and elongated apical cell and prominent foot-shaped basal cells. Apical cells were conically elongated, and basal cells were prominently shaped, 20.1 to 47.2 × 2.5 to 2.9 μm. Macroconidia were produced from monophialides on branched conidiophores. Chlamydospores were abundant in clumps or chains, ellipsoidal to subglobose with thick and roughened walls. The isolated strain was identified morphologically as a member of the Fusarium incarnatum-equiseti species complex (FIESC) (Leslie and Summerell 2006). To confirm identity of FQ01, the internal transcribed spacer (ITS) region and the translation elongation factor 1-α gene (EF1-α) were amplified using primers ITS1/ITS4 (White et al. 1990) and EF1-728F/EF1-986R (Carbone and Kohn 1999), respectively. Total genomic DNA was extracted using the E.Z.N.A. Fungal DNA Mini Kit (Omega Bio-tek) according to the manufacturer’s instructions. Resultant sequences of FQ01 were obtained and submitted to GenBank (MN133053 for ITS and MN133052 for EF1-α). BLASTn analyses showed that sequences shared 99.83% identity with a sequence of F. equiseti for ITS (MH879250.1) and 100% with a sequence of F. equiseti (MK692895.1) for EF1-α. Further BLAST search in the FUSARIUM-ID database indicated that the sequences shared 100% identity with a sequence of FIESC for ITS (FD_01695) and 98.58% with a sequence of FIESC for EF1-α (FD_01627) (Geiser et al. 2004). Pathogenicity tests were conducted on wounded and nonwounded peach fruits with mycelial plugs and conidial suspension, respectively. Ten healthy and surface-sterilized fruits (cv. I9) were wounded using a sterilized needle and inoculated with mycelial discs (6 mm). Ten surface-sterilized nonwounded peach fruits (cv. Qiumi) were sprayed with a 10⁵ conidia/ml suspension. For the control, wounded and nonwounded fruits were inoculated separately using agar plugs and DW, respectively All fruits were enclosed in plastic bags and incubated in a growth chamber at 26°C, 90% relative humidity, and 12 h/12 h light (10,000 lux)/dark. After 5 days, the inoculated sites on wounded fruits had brown and sunken lesions. On nonwounded fruits, brown rot and white fluffy mycelia were observed after 1 week. No symptoms were observed on control fruit. The fungus was reisolated from the lesions of the inoculated plants and verified morphologically. This is the first report of FIESC on peach fruits in China. Owing to the range of mycotoxins produced, members of the FIESC are not only saprophytes and opportunistic plant pathogens but also highly associated with human and animal disease (Leslie and Summerell 2006). The pathogen presents a severe threat to peach quality and production, so further research should be implemented to find effective management strategies to prevent and control it.

There are few studies on nitrogen and phosphorus dispersion from the Yangtze estuary to adjacent Zhoushan sea areas in China. This study conducted numerical calculation to simulate the nitrogen and phosphorus transport from the Xuliujing section of the Yangtze Estuary to Zhoushan coastal areas. The effect of inorganic nitrogen transport was greater than that of active phosphorus. The concentration increment of inorganic nitrogen near Zhoushan, Daishan and Qushan islands were between 0.5mg/L and 0.8mg/L. The concentration increment of active phosphate near Shengsi was about 0.015mg/L, and those in the coastal areas of Qushan, east Daishan and northeast Zhoushan were between 0.015-0.02mg/L. To the north of Qushan Island, the percentage of inorganic nitrogen increment to the background was more than 70%, and those of other coastal areas were about 50%. The percentage of active phosphate increase to the background was more than 70% in the north of Shengsi, and the coastal areas from Dayangshan to Qushan were more than 50%. Daishan Island, East Coastal area and east side of Shengsi accounted for 30%.

The aim of the study was to investigate the influences of micro ribonucleic acid (miR)-21 and downstream Toll-like receptor 4 (TLR4)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway on myocardial apoptosis induced by myocardial ischemia-reperfusion (I/R) injury in rats.Recombinant adeno-associated virus rAAV9-ZsGreen-pre-miR-21 and blank control virus were constructed. A total of 48 Sprague-Dawley (SD) rats were randomly divided into S1 group (open chest only), S2 group (transfection with blank virus + open chest), I/R1 group (transfection with blank virus + 6 d of myocardial I/R), and I/R2 group (transfection with miR-21 + 6 d of myocardial I/R). The cardiac function and myocardial infarct size of rats were evaluated in each group. Quantitative Polymerase Chain Reaction (qPCR) was applied to measure the expression level of miR-21 in the myocardium. The level of myocardial apoptosis in each group was detected through terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) staining. Western blotting was performed to determine the protein expression levels of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein (Bax), Caspase-3, TLR4, and NF-κB in the myocardium. The content of interleukin-6 (IL-6) and IL-10 was measured using enzyme-linked immunosorbent assay (ELISA) kits.The cardiac function of rats in I/R1 and I/R2 groups was significantly lower than that in S1 and S2 groups (p0.01). Rats in I/R2 group had better cardiac function than those in I/R1 group (p0.01). In I/R1 group, the level of myocardial apoptosis of rats was overtly increased compared with that in S1, S2, and I/R2 groups (p0.01), while the expression level of miR-21 in myocardium was evidently lower than that in S1, S2, and I/R2 groups (p0.01). Compared with S1, S2, and I/R2 groups, I/R1 group had markedly decreased Bcl-2/Bax expression level and IL-10 content and overtly elevated expression levels of Caspase-3, p-TLR4, p-NF-κB, and IL-6 content in the myocardium (p0.01).Myocardial I/R injury in rats leads to decreased expression of miR-21. The overexpression of miR-21 is able to effectively inhibit the TLR4/NF-κB pathway and reduce the level of myocardial apoptosis of rats and the release of inflammatory factors.

Chemo-resistance of colon cancer remains a major problem in therapy. The role of miR-215-3p in the chemo-sensitivity of colon cancer remains unidentified.Here, we constructed a 5-Fluoracil (5-Fu) resistant HCT116 cell line (HCT116/5-Fu) and miR-215-3p expression levels were measured in 56 cases of colon cancer tissues and 23 cases of normal tissues by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of miR-215-3p on colon cancer cell growth and apoptosis were investigated using cell counting kit-8 (CCK-8) and apoptosis assay, respectively. In addition, CXC-chemokine receptor type1 (CXCR1) was identified as a target of miR-215-3p by using luciferase reporter assay.miR-215-3p was down-expressed in the 5-FU resistant cell compared to the parent cell. The level of miR-215-3p was correlated with the 5-Fu sensibility of colorectal cancer cell and the alteration of miR-215-3p affected the sensibility of colorectal cancer cells toward 5-Fu. Furthermore, miR-215-3p accelerated the apoptosis of colorectal cancer cell which was treated with 5-Fu. Mechanically, miR-215-3p regulated the level of endogenous CXCR1 in HCT116 cell and alternation of CXCR1 affected the 5-Fu sensibility mediated by miR-215-3p. Finally, overexpression of miR-215-3p restrained the growth of HCT116/5-Fu cells in the xenograft model.MiR-215-3p improved the 5-Fu sensibility via regulating the expression of CXCR1 in the colorectal cancer cell.

Multilocus sequence typing (MLST) was applied to investigate the genetic diversity of Candida albicans in the intestinal tract of cirrhosis patients.We used CHROM agar Candida medium to obtain 105 Candida sp. isolates from fecal samples (276 subjects), including 63 isolates from the cirrhosis group (141 subjects) and 42 isolates from the healthy control group (135 subjects).Among the 105 Candida strains isolated, 60 strains were identified as Candida albicans. Patients with cirrhosis had significantly higher rates of colonization by Candida sp. (44.68% vs. 31.11%, p0.05) and C. albicans (27.66% vs. 15.56%, p0.05) relative to healthy controls. In the cirrhosis group, the rate of colonization further increased with disease progression and antibiotic treatment (p0.01). Sixty C. albicans isolates were analyzed by MLST. Fifty diploid sequence types (DST) were observed, and 26 new DSTs and 3 novel alleles were found. The majority of isolates were distributed among three clades, clade 8 (31.67%), clade 14 (15.00%) and clade 18 (21.67%). Among 39 strains from the cirrhosis group, 16 strains (41.02%) belonged to clade 8, while only 3 strains (14.29%) from healthy group belonged to clade 8 (p0.05). In addition, concatenated sequences of the 7 housekeeping gene fragments were analyzed for all the different DSTs in clade 8 to evaluate the loss of heterozygosity (LOH), which indicates C. albicans microvariation in the gut of cirrhosis patients.This study suggests that cirrhosis disease progression and antibiotic treatment is associated with increased colonization by Candida sp. and C. albicans. We are the first to provide MLST-based genotype profiles for C. albicans Guizhou China, and to identify clade 8 as the potential main clade of C. albicans colonization in the gut of cirrhosis patients.

To investigate the relationship between microRNA-203 (miR-203) and diabetic nephropathy and its potential mechanism.The expression of microRNA-203 in mice with diabetic nephropathy and M4200 cells cultured with high glucose was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Toll-like receptor 4 (TLR4), the target gene of microRNA-203, was predicted and screened by bioinformatics method. Real-time quantitative PCR and Western blot were used to detect the endogenous TLR4 level in renal cortex of db/db mice with diabetic nephropathy and glomerular mesangial cells cultured in high glucose or low glucose. The expression of microRNA-203 and TLR4 mRNA were evaluated by RT-PCR after treatment of miR-203 mimics and inhibitor. The protein of TLR4 level was detected by Western blot. Additionally, the proliferation ability of cells was evaluated by Cell Counting Kit-8 (CCK8). The target relationship between microRNA-203 and TLR4 3' UTR was confirmed by luciferase reporter assay RESULTS: The expression of miR-203 was significantly decreased in the kidney of mice with diabetic nephropathy and M4200 cells cultured in high glucose. On the contrary, TLR4 expression was significantly increased. Results of in vitro experiments showed that miR-203 could bind to 3'UTR region of TLR4. Overexpression of microRNA-203 significantly decreased the levels of TLR4 mRNA and protein. Meanwhile, low expression of miR-203 leaded to increased TLR4 expression, resulting in an enhanced proliferation of M4200 cells.The downregulation of microRNA-203 leaded to an increased level of TLR4, thus promoting proliferation of M4200 cells in the pathogenesis of diabetic nephropathy.

Dengue is the most prevalent and rapidly spreading mosquito-borne viral disease. As a dengue non-endemic country, China has experienced several dengue outbreaks in recent years. However, dengue patients in China displayed distinct clinical characteristics compared to patients in endemic countries. To standardize the diagnosis and treatment of dengue fever, the experts of the Society of Infectious Diseases, Society of Tropical Medicine and Parasitology of Chinese Medical Association, and the Society of Emergency Medicine, China Association of Chinese Medicine have reached this guideline based on guidelines for diagnosis, treatment, prevention and control of dengue (World Health Organization, 2009); guidelines for diagnosis and treatment of dengue (National Health and Family Planning Commission of the People's Republic of China, 2014, Edition 2), health industry standard of the People's Republic of China "diagnosis for dengue fever (WS216-2018)" and systemic reports on dengue. The guideline includes 8 aspects: introduction, terminology, epidemiology and prevention, etiology and pathogenesis, clinical features, diagnosis, treatment and problems to be solved.

N^(6)-methyladenosine (m^(6)A) has been identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, and plays important biological roles in the regulation of cellular metabolic processes. However, its role in myogenic differentiation is unclear. Here, we altered the m^(6)A RNA methylation level by overexpression of METTL3, and explored the effect of m^(6)A RNA methylation on myogenic differentiation of murine myoblasts in vitro. The m6A RNA methylation level is regulated by exogenous methylation inhibitor cycloleucine (Cyc) and methyl donor betaine (Bet). Therefore, chemical reagents of Cyc and Bet were used to test the regulatory effect of m^(6)A RNA methylation on myogenic differentiation. Results showed that METTL3 and Bet positively regulated the m^(6)A RNA methylation levels, and Cyc negatively regulated m^(6)A RNA methylation levels. In addition, m^(6)A methylation positively regulated myogenic differentiation in murine myoblasts. These findings provide insight in the mechanisms underlying the effect of m^(6)A RNA methylation on myogenesis.