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Long non-coding RNA (lncRNA), is essential for the development and progression of cancers. LncRNA regulates target gene expression by sponging the corresponding microRNA (miRNA) during tumorigenesis. This work aimed to explore the role of one lncRNA, ELFN1-AS1, in colorectal cancer (CRC) development and elucidate the pertinent signaling pathway.First, we found that ELFN1-AS1 was highly abundant in the human CRC tissues and cell lines. Silence of ELFN1-AS1 expression reduced cell proliferation, colony formation, migration and invasion, while inducing apoptosis in vitro; moreover, knockdown of ELFN1-AS1 decreased the size and weight of tumor in vivo.Luciferase reporter assay revealed that ELFN1-AS1 interacted with miR-1205 and suppressed its expression. In addition, miR-1205 could bind to the 3' untranslated region (3'-UTR) of Metastasis Associated Protein1 (MTA1) and inhibited ELFN1-AS1 expression. More importantly, overexpression of MTA1 completely rescued the phenotype of ELFN1-AS1 knockdown.In sum, our study demonstrated that ELFN1-AS1 sponges miR-1205 to upregulate MTA1, which is essential for CRC cell proliferation, migration, and invasion as well as apoptosis induction.

Long non-coding RNA (lncRNA) is closely related to the occurrence and development of gastric cancer, but the mechanism and clinical significance of lncRNA AOC4P are still unclear. This study aimed to investigate the expression and function of lncRNA AOC4P in gastric cancer.Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA AOC4P in 80 gastric cancer tissues and adjacent normal tissues. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), flow cytometry and transwell assays were used to study the effects of lncRNA AOC4P on the proliferation, apoptosis, migration and invasion of gastric cancer cells. Western blot was used to detect the related protein level of the mitogen-activated protein kinase (MAPK) signal pathway.The expression of lncRNA AOC4P in gastric cancer tissues was higher than that in adjacent tissues. OS or DFS time were significantly shortened in patients with gastric cancer with high expression of lncRNA AOC4P. Inhibition of lncRNA AOC4P expression can inhibit cell proliferation, migration and invasion, promoting cell apoptosis to some extent. Inhibition of lncRNA AOC4P expression also can result in the decreased expression levels of extracellular-signal-regulated kinase 1 (ERK1), c-Jun N-terminal kinases (JNK) and p38 proteins.High expression of lncRNA AOC4P in gastric cancer may be related to the occurrence, development and prognosis of gastric cancer. LncRNA AOC4P is expected to become a new diagnostic marker and therapeutic target for gastric cancer.

To uncover the biological role of long non-coding RNA (lncRNA) CASC19 in the pathogenesis of non-small cell lung carcinoma (NSCLC) and the potential mechanism.Expression pattern of lncRNA CASC19 in NSCLC tissues and cell lines was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Survival analysis on the correlation between CASC19 level and prognosis of NSCLC patients was conducted by introducing for the Kaplan-Meier estimator. After the transfection of si-CASC19 in A549 and PC9 cells, changes in viability, migratory, and invasive capacities were evaluated. Dual-luciferase reporter gene assay was performed to explore the interaction between microRNA-130b-3p (miRNA-130b-3p) and CASC19/ZEB2. Their interactive effects on the progression of NSCLC were finally investigated through rescue experiments.LncRNA CASC19 was upregulated in NSCLC tissues and cell lines. NSCLC patients with high expression of CASC19 presented a worse survival. Knockdown of CASC19 attenuated proliferative, migratory, and invasive capacities of A549 and PC9 cells. CASC19 sponged miRNA-130b-3p and negatively regulated its level. ZEB2 was the direct target of miRNA-130b-3p. The knockdown of miRNA-130b-3p reversed the regulatory effects of CASC19 on A549 and PC9 cells.CASC19 sponges miRNA-130b-3p to regulate ZBR2 as a ceRNA, thus accelerating the progression of NSCLC by regulating proliferative, migratory, and invasive capacities of tumor cells.

Environmental writing and ecocritical inquiry have been practiced more vigorously in recent years than before, with increasing sophistication and substantial progress. In this study, the discourses of environment in modern fiction are examined from an ecocritical perspective. The literary representations of environment in modern fiction reveal new insights into environmental issues and provide new perspectives and viable documentary information for the scientific study of the environment. Trying to conceptualize some of the environmental phenomena, this study concludes that zoomorphism and anti-anthropocentrism can well balance ecocentric concerns, reflecting and enhancing the close ties and interdependence between human society and the natural world. Environmental apocalypticism is another notable concept conveyed in modern fiction, indicating great crises of the worsening environment. More importantly, environmental apocalypticism serves as an alarming reminder that the remarkable complexity of problematic environmental issues humans facing are both urgent and devastating. With analyses of literature’s engagement with the natural environment, the interdisciplinary vision highlights the interconnections between man and nature, expands research space for both disciplines and provides more efficient means of solving the environmental problems.

The Shanghai Synchrotron Radiation Facility (SSRF) is a third-generation synchrotron radiation light source and will come into commission in April 2009. The project Shanghai Laser Electron Gamma Source (SLEGS), which is a high intensity γ-ray beamline based on Laser Compton Scattering (LCS) between relativistic electron bunches and a laser, has been proposed at the SSRF. According to our simulations, the SLEGS is expected to generate a polarized γ-ray beam of up to 22 MeV and 109–10 photons/s if using 3.5 GeV, 200–300 mA relativistic electrons and a 500 W CO2 polarized laser. Here we describe the status and the application prospects of SLEGS and its developed prototype.

Urbanization has a significant impact on environmental pollution, but the relationship between urbanization and environmental pollution is complex. This paper separately discusses the population urbanization and land urbanization relationship of air pollution, and the impact of urbanization level and land intensive utilization on air pollution are used for empirical analysis. The panel data of 35 provincial capitals and sub provincial cities in China are selected from 2003 to 2015, and index system is constructed to evaluate the level of urbanization, land urbanization and land intensive use. After testing the Heteroscedasticity between panels, Autocorrelation within groups and contemporaneous correlation across panels of the panel data, an econometric model is established and a comprehensive feasible generalized least squares (FGLS) method is chosen to estimate the consistent and valid results which show the effects of urbanization, land urbanization and land intensive utilization on air pollution. This paper puts forward the related questions and policy recommendations.

As a prototype of the Shanghai Laser Electron Gamma Source in the Shanghai Synchrotron Radiation Facility, an x-ray source based on laser-Compton scattering (LCS) has been installed at the terminal of the 100 MeV linac of the Shanghai Institute of Applied Physics. LCS x-rays are generated by interactions between Q-switched Nd:yttrium aluminum garnet laser pulses [with wavelength of 1064 nm and pulse width of 21 ns (full width at half maximum)] and electron bunches [with energy of 108 MeV and pulse width of 0.95 ns (rms)] at an angle of 42 degrees between laser and electron beam. In order to measure the energy spectrum of LCS x-rays, a Si(Li) detector along the electron beam line axis is positioned at 9.8 m away from a LCS chamber. After background subtraction, the LCS x-ray spectrum with the peak energy of 29.1 +/- 4.4 vertical bar(stat)+/- 2.1 vertical bar(syst) keV and the peak width (rms) of 7.8 +/- 2.8 vertical bar(stat)+/- 0.4 vertical bar(syst) keV is observed. Normally the 100 MeV linac operates with the electron macropulse charge of 1.0 nC/pulse, and the electron and laser collision repetition rate of 20 Hz. Therefore, the total LCS x-ray flux of (5.2 +/- 2.0)x10(2) Hz can be achieved.

This paper reports the results of longitudinal surveillance of filariasis for 4 years in Jining City, Shandong, where the disease has been controlled. It showed that the microfilaremia rate dropped from 0.48% to 0.03% and no new case was found and the natural infective rate of the main vector mosquito Culex pipiens fell from 0.45% to 0.03% and no larva III was detected. The purifying measures should put emphasis on the management of migrating persons repeatedly examining and treating old microfilaremia cases, the border areas with high microfilaremia rate, as well as working out a plan to cure the advanced patients and mosquito control measures with special reference to construction of new village settlements.

123I- and 131I-labeled hexadecenoic acid (IHDA, radiochemical purity over 92%, dissolved in 6% bovine serum albumin solution) was investigated in vivo. ICR mice were administered IHDA via the tail vein. Maximum myocardial uptake (27.3 +/- 5.1%) was reached about 0.5 min after the injection. The ratio of uptake in the heart to that in the lungs was 2.3, to that in liver 1.5 and to that in other organs 2.4 to 6.4. The dog myocardium was visualized distinctly within 3-5 min with a gamma camera after i.v. 131I-IHDA, and not interfered with by activities in the lungs, liver and other organs. The low blood levels at 20 min had little effect on the quality of the heart images.

The effects of intramuscular injection of Artemether on peripheral T, B, T mu and T gamma lymphocytes in beagle dogs was investigated cytochemically. Doses used were 6, 19, and 32 MKD, respectively. Results showed that after injection of Artemether for 15 successive days, the T, B, and T mu lymphocytes of the 19 and 32 MKD groups were markedly reduced and the T gamma lymphocytes of all 3 dosage groups were decreased to zero. 28 days after cessation of the drug, T, T mu and T gamma lymphocytes had recovered to control levels, while the B lymphocytes of the 19 and 32 MKD groups remained markedly lower than those of the control groups. The differences and similarities of the effects on the peripheral T, B, T mu and T gamma lymphocytes produced by the 3 dosages of Artemether and the relationship between the changes of T, B lymphocytes and immunological function of beagle dogs are discussed.

Summary 11C-benzoic acid prepared in a radiochemical purity over 90% was studied radiopharmacologically in mice and rabbits. The uptake of 11C-benzoate in ICR mice increased quickly. The ratio of kidney uptake rate to that in other organs reached values between 9 and 55 with a maximum at 10 min after i.v. injection. Gamma camera imaging of rabbits showed that uptake in the kidneys began at 2 min after injection and that activity began to appear in the bladder 4 min later. Rabbits with left renal artery ligature showed no uptake in the left kidney but the right kidney was imaged to the same extent as that of a rabbit without artery ligature. The kidney imaging of 11C-benzoic acid may be a useful method for renal diagnosis.