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The recent discovery of superconductivity in infinite-layer nickelates generates tremendous research endeavors, but the ground state of their parent compounds is still under debate. Here, we report experimental evidences for the dominant role of Kondo scattering in the underdoped Nd1-xSrxNiO2 thin films. A resistivity minimum associated with logarithmic temperature dependence in both longitudinal and Hall resistivities are observed in the underdoped Nd1-xSrxNiO2 samples before the superconducting transition. At lower temperatures down to 0.04 K, the resistivities become saturated, following the prediction of the Kondo model. A linear scaling behavior $\sigma _{{\boldsymbol{xy}}}^{{{\boldsymbol AHE}}}{\rm{\ }}\sim{\rm{\ }}{\sigma }_{{\boldsymbol{xx}}}$ between anomalous Hall conductivity $\sigma _{{\boldsymbol{xy}}}^{{\boldsymbol{AHE}}}$ and conductivity ${\sigma }_{{\boldsymbol{xx}}}{\rm{\ }}$is revealed, verifying the dominant Kondo scattering at low temperature. The effect of weak (anti-)localization is found to be secondary. Our experiments can help clarifying the basic physics in the underdoped Nd1-xSrxNiO2 infinite-layer thin films.

孤立性纤维性肿瘤（solitary fibrous tumor，SFT）来源于表达CD34抗原的树突状间质细胞，具有向成纤维细胞或肌纤维母细胞性分化的潜能，是一种罕见的成纤维细胞间充质的梭形细胞肿瘤。约60%以上SFT发生于胸膜，也可发生于人体任何部位，其中头颈部较罕见。本文报道1例头颈部SFT，结合临床病理表现及国内外相关文献进行回顾性分析，旨在提高临床及病理医师对SFT的诊断水平和认识。.

滑膜肉瘤是一种具有间叶和上皮双相性分化的恶性肿瘤，在细胞遗传学上存在X与18号染色体的特异性易位，滑膜肉瘤可发生在任何部位，发生于颞下窝部位则罕见。根据组织学形态可分为双相分化型、梭形细胞型、单相上皮型和低分化（圆形细胞）型四型，其中单相上皮型、低分化型是较为少见的亚型。本文报道1例颞下窝滑膜肉瘤，经确诊后行肿瘤边缘广泛切除，术后随访6个月未复发。.

Adenosine is an immunosuppressive factor that limits anti-tumor immunity through the suppression of multiple immune subsets including T cells via activation of the adenosine A2A receptor (A2AR). Using both murine and human chimeric antigen receptor (CAR) T cells, here we show that targeting A2AR with a clinically relevant CRISPR/Cas9 strategy significantly enhances their in vivo efficacy, leading to improved survival of mice. Effects evoked by CRISPR/Cas9 mediated gene deletion of A2AR are superior to shRNA mediated knockdown or pharmacological blockade of A2AR. Mechanistically, human A2AR-edited CAR T cells are significantly resistant to adenosine-mediated transcriptional changes, resulting in enhanced production of cytokines including IFNγ and TNF, and increased expression of JAK-STAT signaling pathway associated genes. A2AR deficient CAR T cells are well tolerated and do not induce overt pathologies in mice, supporting the use of CRISPR/Cas9 to target A2AR for the improvement of CAR T cell function in the clinic., Activation of the adenosine receptor A2AR is associated with suppression of T cell function in the tumor microenvironment. To overcome immunosuppression, here the authors show that CRISPR/Cas9 mediated deletion of A2AR enhances CAR T cell effector functions without altering memory or persistence properties, improving CAR-T mediated tumor control in pre-clinical models.

Orthopedic infection is a serious complication in surgeries and remains a great challenge in clinics. Here, the natural antimicrobial compound vanillic acid-loaded gold nanospheres/mesoporous silica nanoparticles (VA@Au-MSNs) were fabricated for chemo-photothermal synergistic therapy to orthopedic infections. The shape and morphology of Au-MSN and VA@Au-MSN were observed by scanning electron microscopy and transmission electron microscopy. The properties of VA@Au-MSN or related components were characterized by dynamic light scattering, thermogravimetric analysis, Brunauer–Emmett–Teller (BET) analysis, and photothermal analysis. Vanillic acid released from VA@Au-MSN was detected in phosphate-buffered saline. A cytotoxicity test and an antibacterial assessment were performed to explore the biosafety and antibacterial activity of VA@Au-MSN, respectively. The results showed that Au-MSN possessed a high BET surface area (458 m2/g). After loading vanillic acid, the BET surface area reduced to 72 m2/g. The loading efficiency of Au-MSN was 18.56%. Under 808 nm laser irradiation, the temperature at the wound site injected with the Au-MSN solution in the mouse increased from 24 to 60 °C within about 12 s. Also, the high temperature could promote the release of vanillic acid from VA@Au-MSN. Additionally, VA@Au-MSN has no obvious cytotoxicity to MC3T3-E1 cells, but the generated local hyperthermia and the VA released from VA@Au-MSN had excellent antibacterial activity against Staphylococcus aureus in a synergistic way. In conclusion, the VA@Au-MSN with biosafety and excellent antibacterial performance might be applied for the treatment of orthopedic infection.

Chimeric antigen receptor (CAR) T cell therapy has been highly successful in hematological malignancies leading to their US Food and Drug Administration (FDA) approval. However, the efficacy of CAR T cells in solid tumors is limited by tumor-induced immunosuppression, leading to the development of combination approaches, such as adjuvant programmed cell death 1 (PD-1) blockade. Current FDA-approved methods for generating CAR T cells utilize either anti-CD3 and interleukin (IL)-2 or anti-CD3/CD28 beads, which can generate a T cell product with an effector/exhausted phenotype. Whereas different cytokine preconditioning milieu, such as IL-7/IL-15, have been shown to promote T cell engraftment, the impact of this approach on CAR T cell responses to adjuvant immune-checkpoint blockade has not been assessed. In the current study, we reveal that the preconditioning of CAR T cells with IL-7/IL-15 increased CAR T cell responses to anti-PD-1 adjuvant therapy. This was associated with the emergence of an intratumoral CD8(+)CD62L(+)TCF7(+)IRF4(–) population that was highly responsive to anti-PD-1 therapy and mediated the vast majority of transcriptional and epigenetic changes in vivo following PD-1 blockade. Our data indicate that preservation of CAR T cells in a TCF7(+) phenotype is crucial for their responsiveness to adjuvant immunotherapy approaches and should be a key consideration when designing clinical protocols.

Adoptive cell therapies using genetically engineered T cell receptor or chimeric antigen receptor T cells are emerging forms of immunotherapy that redirect T cells to specifically target cancer. However, tumor antigen heterogeneity remains a key challenge limiting their efficacy against solid cancers. Here, we engineered T cells to secrete the dendritic cell (DC) growth factor Fms-like tyrosine kinase 3 ligand (Flt3L). Flt3L-secreting T cells expanded intratumoral conventional type 1 DCs and substantially increased host DC and T cell activation when combined with immune agonists poly (I:C) and anti-4-1BB. Importantly, combination therapy led to enhanced inhibition of tumor growth and the induction of epitope spreading towards antigens beyond those recognized by adoptively transferred T cells in solid tumor models of T cell receptor and chimeric antigen receptor T cell therapy. Our data suggest that augmenting endogenous DCs is a promising strategy to overcome the clinical problem of antigen-negative tumor escape following adoptive cell therapy.

Anthracnose is a serious fungal disease that primarily infects strawberry roots and stolons during development. Here, 91 isolates from different areas of Zhejiang province, China, were collected. Morphological characteristics were analyzed, and a phylogenetic analysis based on multiple genes (actin, internal transcribed spacer, calmodulin, glyceraldehyde-3-phosphate dehydrogenase, and chitin synthase) was performed. We found that all of the Colletotrichum species causing strawberry anthracnose belonged to the Colletotrichum gloeosporioides complex. Among them, we identified 48 isolates of C. fructicola, 21 isolates of C. siamense, 13 isolates of C. gloeosporioides, and 9 isolates of C. aenigma. C. siamense was distributed in the central and eastern regions of Zhejiang province (Hangzhou, Jinhua, Shaoxing, Ningbo, and Taizhou). This is the first report of C. siamense causing strawberry anthracnose in Zhejiang province. C. fructicola was the most dominant species causing strawberry anthracnose in Zhejiang province. We identified the four species causing strawberry anthracnose in Zhejiang province, which will improve our understanding of the strawberry anthracnose epidemic and will benefit the development of future control measures.

Central nervous system (CNS) fungal infections are challenging and difficult to diagnose and treat. This article introduces the high risk factors, pathogen spectrum and laboratory indicators that cause CNS fungal infection. As patients with CNS fungal infections are often accompanied by immunodeficiency, it is especially necessary for clinical early detection, early prevention, and early diagnosis, and timely and effective implementation of optimized diagnosis and treatment programs to prevent further deterioration of the disease.中枢神经系统（CNS）真菌感染以病情严重、诊断困难、治疗棘手而具挑战性。本文介绍了导致CNS真菌感染的高危因素、病原谱和实验室指标。由于CNS真菌感染患者常伴有免疫功能低下，尤其需要临床采取有效预防措施，一旦感染则需要早发现、早诊断，及时有效开展优化诊疗方案，预防病情进一步恶化。.

hine Muscat, with its excellent quality and unique Muscat aroma, is emerging as one of the most profitable table grape cultivars in Asia. However, in-depth analysis of the cultivar’s aroma profile before berries are harvest-ready is lacking. The aroma components and their concentrations undergo substantial changes during berry ripening, which provides an opportunity for producing some juice/wine products with special flavour. In this study, the evolution of free and bound aroma compounds in Shine Muscat grapes was analysed from EL-35 to EL-38. The main aroma attributes of harvest-ready berries were: ‘green’, ‘sweet’, ‘floral’ and ‘fruity’. The headspace SPME-GC-MS analysis revealed that alcohols were the most abundant free and bound compounds in Shine Muscat. Furthermore, the free forms of hexanal, β-damascenone, and (E, Z)-2,6-nonadienal determined the aroma characteristics of the grapes directly, while the bound forms of β-damascenone, (E, Z)-2,6-nonadienal, and 2-hexanol determined the aroma of Shine Muscat that cannot to be smelled directly. Taking all indicators together, we believe that picking Shine Muscat grapes at EL-37 (when a minimum TSS of 17% has been reached) maintains its fine aroma characteristics. These results lay the foundation for the further development, utilisation and study of the aroma characteristics of Shine Muscat grapes.

Since distraction osteogenesis had been used in maxillofacial surgery in the 1990s,it has become an important option for treating maxillary hypoplasia in cleft lip and palate (CLP) patients. Numerous domestic and international studies reported that the prognosis of distraction osteogenesis might be affected by various factors, including the design of osteotomy line, type of distractor, advancement of maxillary bone and the age of patients, etc. Therefore, the present article reviewed possible impact factors that affect the effectiveness and outcomes of maxillary distraction osteogenesis of CLP from three aspects: treatment methods, type of patients and adjuvant therapy, which hopefully might benefit the formulation of treatment strategies in clinical work and improve the prognosis of distraction osteogenesis.牵张成骨自20世纪90年代引入口腔颌面外科后，已逐渐成为治疗唇腭裂的重要选择。据研究报道，截骨线的设计、牵张器的类型、上颌骨前移量、手术年龄等因素或将影响牵张成骨术的预后。本文主要针对唇腭裂上颌骨牵张成骨的最新进展，从治疗方法、患者类型和辅助治疗三方面，对可能影响唇腭裂上颌骨牵张成骨疗效和稳定性的相关因素进行阐述，旨在为临床实践中制订恰当的治疗策略，改善手术预后提供参考。.

2021年8月欧洲心脏病学会（ESC）发布了2021版心脏起搏和心脏再同步化治疗指南，对相关的临床证据和临床诊疗规范进行了全面的梳理，为起搏电生理医生的临床实践指明了方向。本文针对此次ESC新指南的更新进行重点介绍，帮助读者了解此次ESC指南的主要进展及其与上一版相比的变化。.

Anoectochilus roxburghii is an important Chinese herbal medicine plant belonging to Orchidaceae and known as Jinxianlian. This orchid is cultivated and mostly adopted to treat diabetes and hepatitis. About 2 billion artificially cultivated seedlings of Jinxianlian are required each year and approximately $600 million in fresh A. roxburghii seedlings is produced in China. From 2011, sporadic occurrence of stem rot on Jinxianlian have been observed in greenhouses in Jinhua City (N29°05', E119°38'), Zhejiang Province. In 2018, nearly 30% of seedlings of Jinxianlian grown in greenhouse conditions were affected by stem rot in Jinhua City. Symptoms initially occurred in the stem at the soil line causing dark discoloration lesions, rotted tissues, wilting, and eventually leading to the death of the plants. A total of 23 diseased seedlings collected from seven different greenhouses were surface sterilized with 1.5% sodium hypochlorite for 3 min, then rinsed in water. Pieces of tissues disinfected from each sample were plated on 2% potato dextrose agar (PDA), and incubated at 25°C in the dark for 5 days (Kirk et al. 2008). A total of 19 isolates were recovered. They developed colonies with purple mycelia and beige or orange colors after 7 days of incubation under 25°C on PDA and carnation leaf agar (CLA) media (Kirk et al. 2008; Zhang et al. 2016). Colonies on PDA had an average radial growth rate of 3.1 to 4.0 mm /d at 25°C. Colony surface was pale vinaceous, floccose with abundant aerial mycelium. On CLA, aerial mycelium was sparse with abundant bright orange sporodochia forming on the carnation leaves. Microconidia were hyaline and oval-ellipsoid to cylindrical (3.7 to 9.3 × 1.3 to 2.9 μm) (n=19). Macroconidia were 3 to 5 septate and fusoid-subulate with a pedicellate base (27.4 to 35.6 × 3.2 to 4.2 μm) (n=19). These morphological features were consistent with Fusarium oxysporum (Sun et al. 2008; Lombard et al., 2019). To confirm the identification based on these morphological features, the internal transcribed spacer region (ITS) and translation elongation factor1 (TEF) were amplified from the DNA of 3 out of 19 isolates chosen at random respectively using the set primer ITS1/ITS4 and EF1/ EF2 (Sun, S., et al. 2018; Lombard et al., 2019). BLAST analysis revealed that the ITS sequences (OK147619, OK147620, OK147621) had 99% identity to that of F. oxysporum isolate JJF2 (GenBank MN626452) and TEF sequence (OK155999, OK156000, OK156001) had 100% identity to that of F. oxysporum isolate gss100 (GenBank MH341210). A multilocus phylogenetic analysis by Bayesian inference (BI) and maximum likelihood (ML) trees based on ITS and TEF indicated that the pathogen grouped consistently with F. oxysporum. Three out of 19 isolates chosen at random were selected to evaluate pathogenicity. Uninfected healthy A. roxburghii seedlings about 40 day-old planted in sterilized substrates were sprayed with distilled water containing 2 x 106 conidia per ml suspensions as inoculums, and plants sprayed with distilled water alone served as controls. Plants were then incubated at 25°C and 85% relative humidity. Ten plants were inoculated for each isolate. After 10 days, all plants inoculated developed stem rot symptoms, while control plants remained healthy. Cultures of Fusarium spp. were re-isolated only from inoculated plants with the frequency of 100% and re-identified by morphological characteristics as F. oxysporum, fulfilling Koch's postulates. To the best of our knowledge, this is the first report of F. oxysporum causing stem rot on A. roxburghii seedlings. As F. oxysporum is a devastating pathogenic fungus with a broad host range, measures should be taken in advance to manage stem rot of A. roxburghii.

毛细胞白血病（HCL）是一种罕见的、具有独特的形态学和免疫表型的成熟小B细胞淋巴组织增殖性疾病，肿瘤细胞侵犯骨髓和脾脏，骨髓活检是最可靠有效的诊断方法，但常因骨髓纤维化、干抽而不能获得满意的标本。HCL为惰性淋巴瘤，临床进展缓慢，在极短时间内伴发噬血细胞综合征则更是罕见，有待对更多病例进行观察，总结经验，提早预防严重并发症的发生。.

To construct and develop the home self-sampling processes of cervical human papillomavirus (HPV) detection and evaluate its application effect. An integrated HPV self-sampling detection platform is scheduled to include three terminals: a user terminal, a detection terminal and a medical terminal. It covers a wide range of functions of self-sampling kit acquisition of user, sample logistics tracking, inspection services, report query, medical consultation, health management, and follow-up tracking. A total of 8 053 users applied for self-sampling kits and all completed online user information registration from January to November 2020. The average age of users ranged from 17 to 84 with a median age of 42 years old. Registered users of the platform were distributed in Jiangsu, Jiangxi, Hebei, Shanxi, Shanghai, Ningxia, Anhui, Zhejiang, Inner Mongolia, Beijing and Xinjiang. 8 045 users completed self-sampling with a kit return rate of 99.9%. Six users lost the kits during the express delivery, and 2 users had the kits contaminated due to improper application; The amount of exfoliated cells collected from 8 045 cases in the sample kits were all within the endogenous internal standard of the nucleic acid kit, and the qualified rate of kits was 100%. The proportion of test report issued by the detection platform within 3 d accounts for 96.93% (7 799/8 054). Among the 763 positive users, 742 completed 6-month reexamination, with a reexamination rate of 97.25%. Unfortunately, 21 cases were lost to follow-up. Taken together, HPV home-based self-sampling is simple, convenient and efficient in use. It can expand the coverage of cervical cancer screening and may help promote HPV screening.探索构建宫颈人乳头状瘤病毒（HPV）检测居家自采样流程并评价其应用效果。搭建一体化的HPV自采样检测平台，此平台有用户端、检测端、医护端3个端口，包含用户获取自采样工具、样本物流跟踪、检验服务、报告查询、医疗咨询、健康管理、随访跟踪等功能。2020年1至11月，平台共有8 053位用户申请自采样标本盒并完成线上信息注册，用户的年龄范围17~84岁、中位年龄42岁。平台注册用户分布在江苏、江西、河北、山西、上海、宁夏回族自治区、安徽、浙江、内蒙古自治区、北京、新疆维吾尔自治区。8 045位用户完成自采样，标本盒回收率99.9%，6位用户因快递运输遗失标本盒，2位用户因使用不当致取样器污染；8 045例标本盒内样本采集到的脱落细胞量均在核酸试剂盒内源性内标之内，标本盒的合格率为100%。平台出具检测报告时间在3 d内的占96.93%（7 799/8 054）。763位阳性用户中742位完成6个月的复查，复查率97.25%，21位失访。综上分析，HPV检测居家自采样流程简单、便捷、高效，可以扩大宫颈癌筛查的覆盖面，可能有助于推进HPV筛查。.