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Objective: To understand the family function, psychological status, and influencing factors of the family members of the frontline medical staff fighting COVID-19. Methods: A psychological questionnaire survey was conducted on the families of 189 clinical front line medical staff who participated in the fight against COVID-19 by using the general information questionnaire, the fam- ily care index scale (APGAR), the generalized anxiety scale (GAD-7), and the depression screening scale (PHQ-9), and the influencing factors were analyzed. Results: The score of family function was 7.00 (5.00, 10.00), 105 individuals (55.6%) had good family function, 72 individuals (38.1%) had moderate family dysfunction, and 12 of them (6.3%) had severe family dysfunction. Multiple linear regression analysis showed that gender, age, educational background, working status during the epidemic, and anxiety level were the influencing factors of the family function status of frontline medical staff (p < 0.05). Conclusion: The family function of the medical staff participating in the clinical frontline fight against COVID-19 is at a medium level. Gender, age, educational background, working status during the epidemic, and anxiety level are the influencing factors of the family function status of this group. The family members of medical personnel have a certain extent of anxiety and depression, so the nursing manager should improve the family function and relieve them of anxiety and depression through psychological counseling and humanistic care, so as to improve their family function.

The numerical stability of the explicit precise algorithm, which was developed for the viscoplastic materials, was analyzed. It was found that this algorithm is not absolutely stable. A necessary but not sufficient condition for the numerical stability was deduced. It showed that the time step in numerical calculation should be less than a certain value to guarantee the stability of explicit precise algorithm. Through a series of numerical examples, the stability analysis on the explicit precise algorithm was proved to be reliable. At last, an iterative algorithm was presented for viscoplastic materials. Both of the theoretical and numerical results showed that the iterative algorithm is unconditionally stable and its convergence rate is rapid. In practice, the explicit precise algorithm and iterative algorithm can be combined to obtain reliable results with the minimum computing costs.

Homozygote carriers of two long (L) alleles of the serotonin transporter (5-HTT) regulatory region displayed in vitro a twofold increase in 5-HTT expression compared with carriers of one or two short (S) alleles. However, in vivo imaging studies yielded contradictory results. Recently, an AG exchange leading to differential transcriptional activation of 5-HTT mRNA in lymphobalstoid cell lines was discovered in the 5-HTT regulatory region. In vitro and in vivo evidence suggests that [(11)C]DASB, a new 5-HTT ligand offers some advantages over the ligands used in previous studies in measuring 5-HTT density independent of synaptic levels of serotonin.We assessed 5-HTT binding potential (BP (2)) in the midbrain of 19 healthy subjects with positron emission tomography and [(11)C]DASB. Accounting for the hypothesized functional similarity of L (G) and S in driving 5-HTT transcription, we assessed whether L (A) L (A) homozygotes display increased midbrain BP (2) compared with carriers of at least one S allele.BP (2) in the midbrain was significantly increased in L (A) L (A) homozygotes compared with carriers of at least one S allele. Interestingly, the genotype effect on the midbrain was significantly different from that on the thalamus and the amygdala where no group differences were detected.This in vivo study provides further evidence that subjects homozygous for the L (A) allele display increased expression of 5-HTT in the midbrain, the origin of central serotonergic projections.

The response to potassium by a vegetable crop,Brassica chinensis Baily (Choy-sam) was studied in field experiments. The results indicated that the petiole and stem contained higher concentrations of potassium than other tissues while the leaf blades and flower contained highest concentrations of nitrogen and phosphorus. Application of potash significantly increased the yield of Choy-sam and the potassium concentration in the plant. There was no response to potash fertilizer when the ammonium acetate soluble K content of the soil was greater than 71 ± 12µg g−1.

Emotional reactivity and regulation are fundamental to human behavior. As inter-individual behavioral variation is affected by a multitude of different genes, there is intense interest to investigate gene-gene effects. Functional sequence variation at two genes has been associated with response and resiliency to emotionally unpleasant stimuli. These genes are the catechol-O-methyltransferase gene (COMT Val158Met) and the regulatory region (5-HTTLPR) of the serotonin transporter gene. Recently, it has been proposed that 5-HTT expression is not only affected by the common S/L variant of 5-HTTLPR but also by an A to G substitution. Using functional magnetic resonance imaging, we assessed the effects of COMT Val(158)Met and both 5-HTT genotypes on brain activation by standardized affective visual stimuli (unpleasant, pleasant, and neutral) in 48 healthy subjects. Based on previous studies, the analysis of genotype effects was restricted to limbic brain areas. To determine allele-dose effects, the number of COMT Met158 alleles (i.e., lower activity of COMT) and the number of 5-HTT low expressing alleles (S and G) was correlated with the blood oxygen level-dependent (BOLD) response to pleasant or unpleasant stimuli compared to neutral stimuli. We observed an additive effect of COMT and both 5-HTT polymorphisms, accounting for 40% of the inter-individual variance in the averaged BOLD response of amygdala, hippocampal and limbic cortical regions elicited by unpleasant stimuli. Effects of 5-HTT and COMT genotypes did not affect brain processing of pleasant stimuli. These data indicate that functional brain imaging may be used to assess the interaction of multiple genes on the function of neuronal networks.

A rapid and high performance gas chromatographic method for the determination of 20 organchlorinated pesticides in oils, fruits and vegetables has been developed. The samples of fruits and vegetables were extracted with petroleum ether-acetone, while those of oils were extracted with acetonitrile-hexane. The extract was purified by Florisil column with ethyl ether-hexane(15:85, V/V) or ethyl ether-petroleum ether(15:85, V/V) as eluent. Chromatographic analysis was performed on wide-bore capillary column with ECD detector. Satisfactory separation and detection of these organchlorinated pesticides are presented with the proposed method. The detection limits of this method were 1.0 ng/g-20.0 ng/g(S/N = 5), the recoveries 83.2%-106.8% and the relative standard deviations 2.0%-9.5%.

To study the effect of tumor necrosis factor alpha (TNFalpha) on intracellular free Ca2+ concentration ([Ca2+]i) and the effects of verapamil (Ver), cyproheptadine (Cyp), and anisodamine (Ani) on TNFalpha-induced [Ca2+]i changes in single endothelial cell, and to explore the mechanisms of TNFalpha-mediated shock and antishock actions of Cyp and Ani.Human umbilical vein endothelial cell strains (ECV304) were seeded in 35-mm tissue culture dish with 2 mL DMEM culture medium. The cultured cells were loaded by Fluo-3/AM. The spatial distribution and the dynamic changes of [Ca2+]i in single endothelial cell were determined by laser scanning confocal microscopy.After stimulation with TNFalpha, [Ca2+]i in single endothelial cell rapidly increased in a concentration-dependent manner and arrived at the peak value within 60 s, afterwards, decreased and kept above the basal level. The confocal scanning image showed that [Ca2+]i elevation was more obvious in nuclear than in cytoplasma and decreased slowly. Ver (1, 2 micromol/L), Cyp (30, 60 micromol/L), and Ani (20, 40 micromol/L) markedly inhibited TNFalpha 1.2 nmol/L-induced [Ca2+]i elevation.TNFalpha markedly induces elevation of [Ca2+]i in a single endothelial cell, it may be an important mechanism of TNFalpha-induced shock and tissue injury. That Cyp and Ani obviously suppress TNFalpha-induced [Ca2+]i elevation probably is one of the mechanisms of their antishock effects.

We reported the efficacy of interval hysteroscopic tubal catherization and hydrotubation for three months in 54 infertile women with tubal obstruction (102 fallopian tubes). By comparison of HSGs before and after treatment, 86 of 102 tubes (84%) showed the improvement of tubal patency (P0.01). 56 tubes were patent (54.9%). This method is effective for partially patent tubes (87.5%), intramural tubal obstruction (62.5%), and the obstruction at the distal portion of the fallopian tube (13.3). One -12 month follow-up after treatment, 12 of 35 women whose tubes became completely patent achieved intrauterine pregnancies.

beta 2-Microglobulin (beta 2-mu) gene-null human melanoma FO-1 cells display lower reactivity with anti-HLA class I monoclonal antibodies (mAb) following transfection with a wild-type mouse beta 2-mu gene (referred to as FO-1C cells) than following transfection with a wild-type human beta 2-mu gene (referred to as FO-1H cells). Furthermore, binding assays with a panel of anti-HLA class I mAb detected higher reactivity of FO-1C cells with mAb TP25.99 than with mAb CR1-S63, CR10-215, CR11-115, TP67, and W6/32 but similar reactivity of FO-1H cells with all the mAb tested. While mAb TP25.99 recognizes a determinant expressed on beta 2-mu-free and beta 2-mu-associated HLA class I heavy chains, the remaining mAb recognize determinants expressed only on beta 2-mu-associated HLA class I heavy chains. The differential effects of mouse and human beta 2-mu on the reactivity with anti-HLA class I mAb of FO-1 cells reflect more than one mechanism. Besides abnormalities in the processing of HLA class I heavy chains associated with mouse beta 2-mu, this molecular complex appears to be unstable on the plasma membrane of FO-1 cells. To analyze the interaction of mouse beta 2-mu with HLA-A and -B antigens, the HLA phenotype of FO-1 cells was determined, using a combination of isoelectric focusing analysis of antigens immunoprecipitated from radiolabeled cells with mAb to monomorphic determinants of HLA class I antigens, binding assays with a limited number of mAb recognizing HLA class I allospecificities, and sequence-specific oligonucleotide probe typing. Although association with mouse beta 2-mu does not cause marked differences in the expression of HLA-A25 and -B8 antigens on the cell surface of FO-1 cells, it causes a selective reduction in the expression of determinants recognized by anti-HLA-A mAb F4/72 and VF19-LL67 and by anti-HLA class I mAb W6/32 on HLA-A25 allospecificities. The differential effect of the association with mouse beta 2-mu on the antigenic profile of HLA-A25 and -B8 antigens may reflect the different characteristics of the amino acids at residue 12, which interact with residue 33 of beta 2-mu. The latter residue is the only one to differ between human and mouse beta 2-mu in the stretch of amino acids interacting with the alpha 1 and alpha 2 domains of HLA class I heavy chains.(ABSTRACT TRUNCATED AT 400 WORDS)

Concanavalin A-induced human and mouse T cell proliferation assay was used to detect the suppressive activity of ascitic fluid (AF) in ovarian cancer patients. About 80% of AF specimens were found to be suppressive. However, when later tested for AF's effect on NK cell activity, instead of suppression, marked enhancement was observed. As IL-2 was barely detectable in AF, attention was focused on interferon (IFN). Its presence was then examined and confirmed by the ability of AF to protect HEp-2 cells from the cytopathic effect of vesicular stomatitis virus (VSV). As the protective effect against VSV was abolished by low pH treatment and by anti-human interferon gamma monoclonal antibodies (MAb), the IFN identified in AF was of the gamma type (IFN-gamma). The MAb could markedly inhibit not only AF's NK-enhancing effect but T cell suppressing effect as well. After removal of the IFN-gamma from AF by affinity chromatography, both activities of AF were lost. The possible clinical implication of this new finding with regards to host's anti-tumor resistance and prognosis is discussed.

Plasma reproductive hormones (testosterone, LH, FSH and prolactin) were measured in 298 normal healthy males aged 30-73 years from rural areas of Sichuan Province, People's Republic of China, and in 505 similar men vasectomized between 1 year and 25 years previously. Age-related increases in LH and FSH but not in testosterone or prolactin were noted in normal men. No adverse effects of vasectomy were observed apart from a 16% increase in mean LH levels in the vasectomized compared to non-vasectomized men of similar ages.The longterm effect of vasectomy on plasma reproductive hormones was investigated in a cross-sectional study conducted in China's Sichuan Province. The study group included 505 men 30-73 years of age who had undergone vasectomy 1-25 years earlier and 298 nonvasectomized controls in the same age group. The mean age of vasectomized men was 48.8 years and that if controls was 46.3 years. The mean duration since vasectomy was 12.4 years in the study group. Study findings revealed a significant increase in plasma testosterone and follicle-stimulating hormone (FSH) with time since vasectomy, but no difference was observed between the vasectomized men and nonvasectomized controls when age effects were controlled. The mean testosterone level in men vasectomized 1-11 years earlier (22.8 nmol/1) was significantly below that for nonvasectomized controls (24.9 mmol/1); however, in men vasectomized 12-15, 16-19, and 20+ years earlier the mean testosterone levels were higher (26.4, 24.6, and 29.0 mmol/1, respectively). Plasma luteinizing hormone (LH) levels increased with age in both groups, but the levels did not differ according to time since vasectomy. The rise in plasma LH, without an associated decrease in testosterone levels, in healthy men suggests either that the aging testis requires higher concentrations of gonadotropins to maintain its testosterone biosynthetic efficiency or that the biological to immunological ratio of the circulating LH may decrease with age.

The longterm effects of vasectomy on the pituitary-gonadal axis were studied in 804 commune members between 30-65 years of age in Chengdu, Sichuan province. The 515 vasectomized individuals operated on between 3-25 years previously were compared with 289 age-matched unvasectomized volunteers. No significant alterations were observed in serum testosterone concentrations in those men ages 30-65 who had been vasectomized between 3 and 20 years ago. The serum LH and FSH ooncentrations in those aged 30-39 who had been vasectomized 5-10 years previously had significant alterations, but these changes returned to normal with increasing age and passage of time. Thus, minimal endocrine effects as a result of vasectomy were observed in subjects between the ages of 30-65 who had undergone vasectomy in the last 20 years. (author's modified)

Augmented growth of tumor cells of both syngeneic and allogeneic origin was often observed when these cells were cocultured with the peritoneal inflammatory macrophages of C57BL/6 mouse, induced by thioglycollate in vitro. While large doses of macrophage-activating factor (MAF) were required for activating these inflammatory macrophages to exert the tumoricidal activity, and much less (suboptimal) doses of MAF were required for activating their cytostatic effect on tumor cells. Whether the macrophage inhibits or enhances the tumor cell proliferation, depends not only on the degree of the activation of macrophage, but also on the macrophage/tumor cell ratio.