1. In vitro storage of Xanthosoma spp. under minimal growth conditions
- Author
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E.A. Zandvoort, M.J.H. Hulshof, and G. Staritsky
- Subjects
Germplasm ,biology ,Agronomie ,Xanthosoma ,Xanthosoma brasiliense ,mannitol ,Horticulture ,Xanthosoma sagittifolium ,low temperature ,biology.organism_classification ,Araceae ,Agronomy ,growth reduction ,Botany ,transfer regime ,medicine ,Subculture (biology) ,Mannitol ,Incubation ,medicine.drug - Abstract
Xanthosoma germplasm requires regular subeulturing if stored in vitro under optimal culture conditions. An experiment was carried out to investigate whetherXanthosoma can be stored continuously under minimal growth conditions. Growth was reduced by incubation at low temperatures (9°C, 13°C and 17°C) and by adding mannitol (3.0% w/v) to the medium. Three investigatedXanthosoma species,Xanthosoma brasiliense, Xanthosoma robustum andXanthosoma sagittifolium could be stored in the dark for at least two years at 13°C. Addition of mannitol to the medium resulted in higher survival rates after three years of continuous storage. The storage period could be extended to at least 48 months forX. robustum andX. sagittifolium by annual subculture and regrowth under optimal culture conditions.
- Published
- 1994
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