Search

Choloepus didactylus (two-toed sloth) is a Xenarthran mammal whose morphology has so far been little studied. Given the increasing number of sloths requiring clinical and surgical interventions, this lack of clarity regarding anatomical features and landmarks has had a negative impact on veterinary decision-making for this species. We therefore sought to describe the topography of the medullary cone (MC) of Choloepus didactylus in order to provide guidance to qualified professionals on the ideal access for locoregional anesthesia. In evaluating four specimens, radiographs and dissections revealed that Choloepus didactylus has three lumbar vertebrae and five sacral vertebrae. The lumbar intumescence is located between T14 and the cranial half of L2, the medullary cone is located between the caudal half of L2 and L3, with an average length of 2.26 cm, and the cauda equina extends from S1 to S5. Based on these anatomical findings, we propose that the lumbosacral region would be the most suitable target for epidural anesthesia in Choloepus didactylus. [ABSTRACT FROM AUTHOR]

Nothrotherium maquinense, an extinct sloth species that existed alongside other megafauna during the Pleistocene, has been a subject of intense debate regarding various aspects of its life. This study integrates an examination of specific forearm bone fracture (paleopathology) with forelimb functional indices (paleoecology) in N. maquinense in order to infer its likely mode of locomotion and substrate preferences. Our findings provide compelling evidence suggesting that N. maquinense possessed climbing abilities and likely had a semi-arboreal lifestyle. Additionally, we report the first occurrence of calcium pyrophosphate deposition disease (CPPD) in this species, indicating that this type of arthritic condition was present in this sloth. [ABSTRACT FROM AUTHOR]

Local ecological knowledge (LEK) can be a valuable source of information about species' biology and their conservation, especially for threatened and poorly known species, such as the Brazilian three-banded armadillo (Tolypeutes tricinctus). Here, we gathered information about the natural history and human–fauna relationships of T. tricinctus based on the LEK of residents from a rural community in northeast Brazil, where a population of T. tricinctus has been recently discovered. To understand LEK, we used participatory transect walks, semi-structured interviews, and a member-checking workshop. T. tricinctus was considered a locally abundant and widespread species, primarily nocturnal, highly popular, and not among the most hunted species. Our findings have important implications for the understanding of T. tricinctus biology and conservation. First, they show that, although T. tricinctus is highly threatened by hunting, there are still localities where it is not among the most hunted species, where it may still be common. Second, as T. tricinctus is a locally common and popular species, research and participatory science projects may be feasible in the area. Moreover, to increase sampling effectiveness, research projects can consider aspects of T. tricinctus biology identified in this study. In conclusion, our findings reinforce the importance of ethnobiology assessments to improve knowledge about species' biology and inform local research and conservation efforts. [ABSTRACT FROM AUTHOR]

Mylodon darwinii is an extinct terrestrial species of sloth with a wide fossil record in South America, the record of the skin and hairs found from "La Cueva del Milodón", Chile. More than a century after the first studies on its hair, we aimed to redescribe the morphological characteristics of the hairs of M. darwinii by applying modern protocols of trichology and microscopy. Hair patterns were also compared with those of two other extinct species of sloths available in the literature. Hairs from mummified specimen housed in Museo de La Plata (Argentina) were analyzed through light and scanning electron microscopy to identify their cuticular and medullary patterns. Guard hairs lack medulla and have a wavy cuticle with irregular scale orientation, while underhairs have an amorphous cuticle pattern and no medulla. Hence, the former ones help in the diagnosis of the hair morphology of the species, while the latter ones do not. Differences found in the final product of each microscopy technique are discussed and should be considered in future research. The outcomes presented herein are a step further toward the understanding of evolutionary processes within Xenarthra, providing important data for such a poorly studied group that has only been recorded in Central and South America. [ABSTRACT FROM AUTHOR]

Relationships between species and their habitat are strongly related to their feeding habits making its description essential for understanding the species' biology and plan their conservation. This study describes the diet of giant anteaters (Myrmecophaga tridactyla), a specialist insectivorous mammal, and evaluates potential sex-related intraspecific variation. We analyzed fecal matter from live individuals and from road-kills (N = 29 individuals) collected from October 2018 to April 2020 in the Cerrado savanna of Mato Grosso do Sul, Brazil. Anteater's diet was described by the relative frequency (RF; proportion of the sample mass represented by the prey item) and the frequency of occurrence (FO; proportion of samples in which the item was detected) of each prey item, which was classified to the lowest taxonomic level possible. The diet was composed of 14 morphotypes of termites (Blattodea), 28 morphotypes of ants (Formicidae) and grass seeds (Poacea). They fed primarily on termites (FO = 89.65%; RF = 75.85%) and ants (FO = 100%; RF = 23.08%), with no significant differences between sexes. Termites of the genus Syntermes (RF = 75.55%) represented most of the dietary sample mass, and ants of the genus Pheidole represented the most frequent item found in the samples (FO = 96.55%). M. tridactyla is a myrmecophagous specialist that consumes a great richness of prey species associated with multiple habitats. Hence, the preservation of the natural heterogeneity of the Cerrado landscape and their habitats is essential for the conservation of this vulnerable species. [ABSTRACT FROM AUTHOR]

The lesser anteater Tamandua tetradactyla is possibly the most common member of the order Pilosa and occurs in almost all tropical environments in South America. Although commonly recorded in mammal inventories, this species is not abundant, and little is known about its ecology. The food habits of the species are mainly based on ants, but the type and contribution of other arthropods to the diet, especially termites, remains a subject little discussed in the literature. In the present study, we carried out a comprehensive assessment of stomach contents of 16 roadkilled anteaters found in grassland ecosystems in the Brazilian Pampa. We found 73,890 prey, with ant and termite species being the most representative dietary resources. Ants were present in 100% of the stomachs and represented more than 85% of all items consumed, totaling 34 species. Termites were present in 62.5% of the stomachs and represented 14.6% of all items consumed and, summed up five species. We also found six other arthropod taxa but with an insignificant proportion of the biomass intake. Our results contribute to increase knowledge on T. tetradactyla feeding habits in the subtropical grasslands of Southern Brazil. [ABSTRACT FROM AUTHOR]

Copyright of Biotropica is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

ABSTRACT This study presents a macroscopic description of the brachial plexus in the royal sloth, Choloepus didactylus, and the common sloth, Bradypus variegatus, to identify the points for anesthetic block to perform surgical procedures. Six C. didactylus and nine B. variegatus had their forelimbs dissected, exposing the brachial plexus, and the points for blockage were located subsequently. In general, the formation of the brachial plexus in C. didactylus involved C5-T1 nerves, whereas the brachial plexus in a few individuals of the species involved C4-T2. In B. variegatus, the brachial plexus was contributed by C6-T2. In both species, the ramifications gave rise to three trunks that joined together to form a common brachial plexus trunk. In the brachial plexus block technique, four accesses were performed: supraclavicular, infraclavicular, axillary, and distal. The results of this study contribute to surgical and anesthetic procedures and research on comparative anatomy among wild animals.

Cabassous tatouay occurs in moist forests, savannas, and grasslands of south, central, and northeastern Brazil, eastern Paraguay, northeastern Argentina, and Uruguay. Despite its wide distribution range, C. tatouay is scarcely recorded in some regions. This study aimed to: present new records of C. tatouay; compile the localities where the species was recorded according to published scientific accounts; and model its distribution range along South America. We present five new records of C. tatouay from Goiás and Minas Gerais states, Brazil. The review of published accounts resulted in 99 studies, which provided 279 occurrences of C. tatouay to Brazil, Argentina, Paraguay, and Uruguay. The great majority of records are from Brazil, mainly from the Atlantic Forest (119), Cerrado (52), and Pampas (49) biomes. Most records from the Cerrado are placed close to or in transition zones with the Atlantic Forest. Five records are within the Caatinga, but restricted to humid montane forest enclaves. The species distribution models indicate that C. tatouay is probably absent to Amazonia and Pantanal. The higher suitability for the species occurs in eastern Paraguay, northeastern Argentina, and south central Brazil, specifically in the Pampas, Atlantic Forest, and the southern part of the Cerrado. [ABSTRACT FROM AUTHOR]

Establishing new somatic cell cultures has raised significant attention as an effective and convenient way to preserve genetic samples for different applications. Although many lines have been established in model animals, none derived from six-banded armadillo species is currently available. We report the successful isolation and characterization of fibroblasts from six‐banded armadillos, evaluating the cell quality after extended culture and cryopreservation. Initially, we collected ear skin from five captive adult individuals and identified fibroblast lines by morphology, karyotyping, and immunophenotyping assays. The isolated fibroblasts were evaluated after several passages (fourth, seventh, and tenth passages) and cryopreservation by slow freezing. Cell morphology, viability, metabolism, proliferative activity, mitochondrial membrane potential, and apoptosis levels were analyzed. The skin explants had great adhesion, and cell outgrowth could be seen after 3–6 d. The cells were verified as fibroblasts at the fourth passage by vimentin expression and normal karyotype (2n = 58). The viability remained high (> 87%) and constant from the fourth to the tenth passage (p > 0.05). The passages did not change the cell morphology and metabolic and growth rates. Moreover, cryopreservation did not affect most evaluated parameters; post-thawed cells maintained their viability, growth, metabolism, and apoptosis levels. Nevertheless, cryopreservation increased mitochondrial membrane permeability and cell population doubling time compared to non-cryopreserved cells (p < 0.05). In summary, viable fibroblasts can be obtained from six-banded armadillo skin while conserving their quality as the number of passages increases and featuring few changes after cryopreservation. [ABSTRACT FROM AUTHOR]

Simple Summary: This article demonstrates that computed tomography combined with the use of contrasts for imaging examinations can provide a general and comprehensive topographic view of the vasculature, structures, and organs in frozen and glycerinated cadavers of Bradypus variegatus. The objective is to present an alternative technique to the anatomical study of preserved frozen and glycerinated cadavers using computed tomography. The study concludes by highlighting that computed tomography allowed a general and comprehensive view of the anatomical structures of frozen and glycerinated cadavers of B. variegatus, such as the topographic location of bone structures, organs, and vessels, with soft tissues better visualized after intravenous or oral administration of contrast. Bradypus variegatus has unique anatomical characteristics, and many of its vascular and digestive tract aspects have yet to be clearly understood. This lack of information makes clinical diagnoses and surgical procedures difficult. The aim of this study was to evaluate the anatomical aspects of frozen and glycerinated corpses of B. variegatus using computed tomography (CT), emphasizing vascular and digestive contrast studies. Nine corpses that died during routine hospital were examined via CT in the supine position with scanning in the craniocaudal direction. In frozen cadavers, the contrast was injected into a cephalic vein after thawing and, subsequently, was administered orally. In addition to bone structures, CT allowed the identification of organs, soft tissues, and vascular structures in specimens. Visualization of soft tissues was better after contrast been administered intravenously and orally, even without active vascularization. Furthermore, the surfaces of the organs were highlighted by the glycerination method. With this technique, it was possible to describe part of the vascularization of the brachial, cervical, thoracic, and abdominal regions, in addition to highlighting the esophagus and part of the stomach. CT can be another tool for the evaluation of B. variegatus cadavers by anatomists or pathologists, contributing to the identification of anatomical structures. [ABSTRACT FROM AUTHOR]

This report is a continuation of the work published a decade ago on the status of specimens assigned in the literature and museum records to the megalonychid sloth Eucholoeops from the early--middle Miocene Santa Cruz Formation (- 18 to 15.6 Ma, Santacrucian Age) of Argentine Patagonia. This previous report concluded that several species (i.e., E. latirostris, E. externus, and E. curtus) are junior synonyms of E. ingens, but the authors were unable to analyze several other species and remains assigned to this genus. Here, analysis of these other species and remains suggest that E. fronto and E. lafonei are also junior synonyms of E. ingens. Included in the current report is a specimen recently recovered from the field of E. ingens that preserves, for the first time, an ectotympanic of Eucholoeops. In addition to the mainly cranial skeletal features analyzed here, consideration of postcranial remains indicate that the medial and lateral distal articular condyles of the femur are both contiguous with the patellar trochlea, in contrast to the previously reported condition of a separate lateral articular condyle in E. ingens. The authors further conclude that several features of E. latifrons suggest that this species is indeed distinct from E. ingens and that some specimens assigned in the literature to E. ingens cannot be so assigned with confidence. [ABSTRACT FROM AUTHOR]

The giant anteater (Myrmecophaga tridactyla) is a vulnerable species in South America and is considered endangered or near extinction in Central America. Therefore, studies describing the reproductive characteristics of this species are pivotal for its conservation. Thus, this study aimed to provide a morphological description of the female reproductive tissues of this species. We collected tissue samples from six female giant anteaters and performed gross, morphological, and histochemical analyses. Five adult subjects and one juvenile were included in the study. In the ovary, classifications were made according to the follicle and oocyte sizes: primordial, primary, secondary, early antral, or antral. Typical follicles with a single oocyte surrounded by a simple or stratified layer of cubic epithelium, atretic follicles, corpora lutea, corpora albicans, and ovarian cysts were also observed. No ovarian lesions were observed. By contrast, endometritis, metritis, mucometra, and endometrial cysts were identified in the uterus. Uterine alterations in these subjects were frequent and could affect reproduction. [ABSTRACT FROM AUTHOR]

A new species of nematode parasite of the genus Delicata (Molineidae: Anoplostrongylinae) is described from the small intestine of a road-killed Greater Naked-tailed Armadillo Cabassous tatouay (Cingulata: Chlamyphoridae) on the BR-040 highway in Rio de Janeiro state, Brazil. The genus Delicata includes 13 species of parasitizing armadillos and anteaters distributed in Brazil, Argentina, and Trinidad and Tobago. The present species is distinguished from almost all species of Delicata by the longest length of the body, except for D. khalili and D. appendiculata. However, these can be distinguished from each other by the length of the spicules. The species that closely resembles, Delicata tatouay n. sp. is D. speciosa, but it can be distinguished by a robust branch from rays 2 and 3, rays 4 larger, and rays 8 longer compared to those of the new species. The new species is the only one with a tail, characterized by a terminal spine with rattlesnake tail-like transversal striations. [ABSTRACT FROM AUTHOR]

Combining morphological and molecular characters through Bayesian total-evidence dating allows inferring the phylogenetic and timescale framework of both extant and fossil taxa, while accounting for the stochasticity and incompleteness of the fossil record. Such an integrative approach is particularly needed when dealing with clades such as sloths (Mammalia: Folivora), for which developmental and biomechanical studies have shown high levels of morphological convergence whereas molecular data can only account for a limited percentage of their total species richness. Here, we propose an alternative hypothesis of sloth evolution that emphasizes the pervasiveness of morphological convergence and the importance of considering the fossil record and an adequate taxon sampling in both phylogenetic and biogeographic inferences. Regardless of different clock models and morphological datasets, the extant sloth Bradypus is consistently recovered as a megatherioid, and Choloepus as a mylodontoid, in agreement with molecular-only analyses. The recently extinct Caribbean sloths (Megalocnoidea) are found to be a monophyletic sister-clade of Megatherioidea, in contrast to previous phylogenetic hypotheses. Our results contradict previous morphological analyses and further support the polyphyly of "Megalonychidae," whose members were found in five different clades. Regardless of taxon sampling and clock models, the Caribbean colonization of sloths is compatible with the exhumation of islands along Aves Ridge and its geological time frame. Overall, our total-evidence analysis illustrates the difficulty of positioning highly incomplete fossils, although a robust phylogenetic framework was recovered by an a posteriori removal of taxa with high percentages of missing characters. Elimination of these taxa improved topological resolution by reducing polytomies and increasing node support. However, it introduced a systematic and geographic bias because most of these incomplete specimens are from northern South America. This is evident in biogeographic reconstructions, which suggest Patagonia as the area of origin of many clades when taxa are underrepresented, but Amazonia and/or Central and Southern Andes when all taxa are included. More generally, our analyses demonstrate the instability of topology and divergence time estimates when using different morphological datasets and clock models and thus caution against making macroevolutionary inferences when node support is weak or when uncertainties in the fossil record are not considered. [ABSTRACT FROM AUTHOR]

Simple Summary: Anteaters are insectivores, consuming diets consisting primarily of termites and ants in the wild; therefore, feeding them under human care has been a challenge and frequently leads to nutritional disorders. Through analysis of free-ranging giant anteaters' stomach content, the findings of this study provide valuable information for optimizing anteater nutrition in zoological institutions and rehabilitation centers causing a significant impact on the welfare of the species. Using the stomach contents of 24 wild road-killed giant anteaters as proxies for their diet, we found that estimated wild diets were much lower in calcium (about ten times less) and phosphorus compared with manufactured feeds commonly fed to anteaters under human care. Indicators of soil consumption, such as ash, iron, and manganese were higher in the stomach contents than in either wild termites or manufactured diets, likely due to high levels of soil ingestion during feeding in the wild. Protein and fat levels in insects, stomach contents, and commercial diets all met carnivore recommendations. Both giant anteaters and tamanduas in managed care often develop hypercalcemia, perhaps because these taxa have an enhanced ability to retain calcium allowing them to survive on such low calcium diets. Results from this study indicate that, for anteaters in managed care, it is important to keep dietary calcium and vitamins D and K within recommended levels to prevent nutritional diseases such as hyper- and hypocalcemia and vitamin K deficiency. [ABSTRACT FROM AUTHOR]

The sloths that dispersed into Central and North America were most likely derived from tropical taxa, as indicated by their high occlusal surface area (OSA) in comparison with body mass, as in South American tropical species. Relative muzzle width and hypsodonty indices were used to infer the dietary adaptation of several Late Pleistocene/Early Holocene giant ground sloths from the Americas. Nothrotheriidae and Megatheridae taxa were adapted to a browser or browsing mixedfeeder diet, Mylodontidae taxa to a variety of dietary types, and Megalonychidae taxa to a browsing mixedfeeder diet. However, these are generalized dietary adaptations and do not necessarily reflect the consumption of specific food resources. In general, Megalonychidae and Nothrotheriidae were climbing species, Mylodontidae were digging species, and Megatheriidae were fully terrestrial species. [ABSTRACT FROM AUTHOR]

The auditory brainstem response (ABR) to tone burst stimuli of thirteen frequencies ranging from 0.5 to 48 kHz was recorded in the nine-banded armadillo (Dasypus novemcinctus), the only extant member of the placental mammal superorder Xenarthra in North America. The armadillo ABR consisted of five main peaks that were visible within the first 10 ms when stimuli were presented at high intensities. The latency of peak I of the armadillo ABR increased as stimulus intensity decreased by an average of 20 ms/dB. Estimated frequency-specific thresholds identified by the ABR were used to construct an estimate of the armadillo audiogram describing the mean thresholds of the eight animals tested. The majority of animals tested (six out of eight) exhibited clear responses to stimuli from 0.5 to 38 kHz, and two animals exhibited responses to stimuli of 48 kHz. Across all cases, the lowest thresholds were observed for frequencies from 8 to 12 kHz. Overall, we observed that the armadillo estimated audiogram bears a similar pattern as those observed using ABR in members of other mammalian clades, including marsupials and later-derived placental mammals. [ABSTRACT FROM AUTHOR]

The study aimed to evaluate metabolic parameters, nutrient intake, and absorption of two diets formulated for Dasypus novemcinctus armadillos under human care. Were studied two diets: D1—a diet with dry dog food, ground beef, and boiled chicken egg with shells; D2—a diet with the same ingredients as D1, with added banana and papaya. Both are mixed in water. The parameters analyzed were body weight (BW), weight gain (WG), maintenance energy requirement (MER), Lee index, biometrics, body condition score, glucose, triglycerides, total cholesterol, and cholesterol fractions for eight male armadillos. Dietary intake (DI) and efficiency, nutrient intake, metabolizable energy, and digestibility coefficient for the diets were evaluated in six male armadillos. The diet that included fruits showed higher BW, WG, MER, Lee index, and better glucose metabolism. Both diets promoted increases in WG and Lee Index, as well as improvements in glucose metabolism. The diet without fruit improved the lipid profile of the animals. D2 presented the highest DI, better dietary efficiency, and higher energy intake but also a lower crude fiber intake. However, it showed the best utilization of gross fiber and all other nutrients. In conclusion, the diets constituted an adequate nutritional option for captive armadillos and can be used in malnutrition and pathological processes recovery. Although both diets were adequate, the nonfruit diet was preferable due to the improved lipid profile. Research Highlights: Studied diets promote intake increase and weight gain in armadillos. Fruits inclusion improves dietary use, digestibility, and glycemic profile but does not improve lipid profile. Nonfruit diet improves lipid profile. [ABSTRACT FROM AUTHOR]

Abstract The progressive fragmentation and loss of habitats represent the main threats for endangered species, causing genetic consequences that may have potential implications for a population’s long-term persistence. Large mammals are the most affected species among vertebrates. The giant armadillo Priodontes maximus is a large South American mammal threatened species, showing nocturnal, solitary and fossorial behavior, occurring at low population densities, and its population dynamics are still poorly known. In this study, we carried out the first assessment of genetic variability and population genetic structure of the species, using a panel of 15 polymorphic microsatellites developed by high-throughput genome sequencing. The spatial Bayesian clustering, Fst and Dest results indicated the presence of two genetic clusters (K = 2) in the study area. These results suggest a reduction in gene flow between individuals inhabiting the Brazilian savanna (Cerrado) and the Pantanal wetlands, with the increased human-driven habitat modifications possibly contributing for this scenario. A bottleneck signal was detected in both populations, and a subpopulation structuring in the Cerrado may also be reflecting consequences of the extensive habitat modifications. Findings from this study provide important and useful information for the future maintenance of genetic diversity and long-term conservation of this flagship species.

ABSTRACT The giant anteater (Myrmecophaga tridactyla) is classified as vulnerable to extinction. Here, we report the occurrence of ancylostomiasis in a young male Myrmecophaga tridactyla. The animal was raised from a puppy in captivity with management directed toward rehabilitation and monitored soft release. Five months after release, significant weight loss was noted in the individual, who was captured for examinations, with detection of Ancylostoma sp. in coproparasitological analysis. Although common in domestic carnivores, this is the first report of hookworm disease in free-ranging giant anteaters. Due to the stress factor arising from adapting to the challenges in the new environment, long-term post-release monitoring of animals undergoing rehabilitation is extremely important to increase the chance of survival of individuals. The giant anteater was released after treatment with vermifuge composed of fenbendazole, pyrantel pamoate, and praziquantel.

A new species of nematode parasite of the genus Delicata (Molineidae: Anoplostrongylinae) is described from the small intestine of a road-killed Greater Naked-tailed Armadillo Cabassous tatouay (Cingulata: Chlamyphoridae) on the BR-040 highway in Rio de Janeiro state, Brazil. The genus Delicata includes 13 species of parasitizing armadillos and anteaters distributed in Brazil, Argentina, and Trinidad and Tobago. The present species is distinguished from almost all species of Delicata by the longest length of the body, except for D. khalili and D. appendiculata. However, these can be distinguished from each other by the length of the spicules. The species that closely resembles, Delicata tatouay n. sp. is D. speciosa, but it can be distinguished by a robust branch from rays 2 and 3, rays 4 larger, and rays 8 longer compared to those of the new species. The new species is the only one with a tail, characterized by a terminal spine with rattlesnake tail-like transversal striations.

The giant anteater (Myrmecophaga tridactyla) is a vulnerable species in South America and is considered endangered or near extinction in Central America. Therefore, studies describing the reproductive characteristics of this species are pivotal for its conservation. Thus, this study aimed to provide a morphological description of the female reproductive tissues of this species. We collected tissue samples from six female giant anteaters and performed gross, morphological, and histochemical analyses. Five adult subjects and one juvenile were included in the study. In the ovary, classifications were made according to the follicle and oocyte sizes: primordial, primary, secondary, early antral, or antral. Typical follicles with a single oocyte surrounded by a simple or stratified layer of cubic epithelium, atretic follicles, corpora lutea, corpora albicans, and ovarian cysts were also observed. No ovarian lesions were observed. By contrast, endometritis, metritis, mucometra, and endometrial cysts were identified in the uterus. Uterine alterations in these subjects were frequent and could affect reproduction.

The auditory brainstem response (ABR) to tone burst stimuli of thirteen frequencies ranging from 0.5 to 48 kHz was recorded in the nine-banded armadillo (Dasypus novemcinctus), the only extant member of the placental mammal superorder Xenarthra in North America. The armadillo ABR consisted of five main peaks that were visible within the first 10 ms when stimuli were presented at high intensities. The latency of peak I of the armadillo ABR increased as stimulus intensity decreased by an average of 20 μs/dB. Estimated frequency-specific thresholds identified by the ABR were used to construct an estimate of the armadillo audiogram describing the mean thresholds of the eight animals tested. The majority of animals tested (six out of eight) exhibited clear responses to stimuli from 0.5 to 38 kHz, and two animals exhibited responses to stimuli of 48 kHz. Across all cases, the lowest thresholds were observed for frequencies from 8 to 12 kHz. Overall, we observed that the armadillo estimated audiogram bears a similar pattern as those observed using ABR in members of other mammalian clades, including marsupials and later-derived placental mammals.

Anthropogenic disturbances affecting forest areas can increase disease prevalence and susceptibility in several species of arboreal mammals, such as sloths. Thus, this study aimed to evaluate the most common conditions in body systems of free-ranging sloths admitted at the Wildlife Triage and Rehabilitation Center of Amazonas of the Institute of Environment and Renewable Natural Resources. A total of 227 individuals (139 retrospective cases from 2015 to 2019, and 88 prospective cases from July 2020 to July 2021) were evaluated over a six-year period. Cases from the genus Bradypus showed involvement of the following body systems: 44% respiratory, 29% integumentary, 15% musculoskeletal, 5% digestive, 3% visual, 2% auditory, 1% circulatory and 1% genitourinary, while that distribution in the genus Choloepus was: 39% musculoskeletal, 27% integumentary, 19% respiratory, 9% digestive, 3% circulatory, 1% visual, 1% auditory and 1% genitourinary. The results reveal significant differences between the condition detected and the genus (Bradypus and Choloepus), age and case outcome. These results can provide data for future investigations of sloth diseases, confirming lesions, as well as motivating and suggesting adequate management methods. [ABSTRACT FROM AUTHOR]

Copyright of Acta Veterinaria Brasilica is the property of Acta Veterinaria Brasilica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We inferred the annual isotopic diet (δ13C) of an individual of the giant ground sloth Eremotherium laurillardi found in Toca dos Ossos (Ourolândia, Bahia, Brazil) through the extension of its third inferior molar. This individual lived in the region at 40,779-39,617cal yr BP. One year of its life was recorded in a length of 67 mm in the tooth. Two years were recorded in this molariform, during which the diet and climate did not change much, and substantial precipitation occurred during the middle of the year, which is in opposition to the modern pattern. The mean carbon (μδ13C=-13.9 ± 1.8‰) and oxygen (μδ18O = 22.5 ± 2.9‰) isotopic values were similar to values for other individuals of the species found in the same cave but different from the values found in other localities of the Brazilian Intertropical Region, which allows us to suggest that this region had more precipitation and lower temperatures in comparison to today. The oxygen isotopic values found in dated fossils of E. laurillardi and from two other taxa found in the same cave (Toxodon platensis, and Notiomastodon platensis) could help in the understanding of the climatic variation that occurred in the region. [ABSTRACT FROM AUTHOR]

The pelvis, formed by the ilium, ischium and pubis, forms the coxal, which is the largest of the flat bones of the skeleton, with an important role in the physiology of birth. Vastly studied in domestic animals, there is still much to be investigated regarding the anatomical classification in wild animals, mainly aiming to improve the understanding of the reproductive management of the species. Tamandua tetradactyla is one of these species in which morphological studies are still scarce, especially when it comes to reproductive aspects. In this context, we carried out the pelvimetry of T. tetradactyla using radiographic images to anatomically and obstetrically classify the pelvis of this species, classifying it as dolicopelvic, with evidence of homoscedasticity of the samples. Verification of the existence of a relationship between the variables studied by the statistical method of Pearson coefficients showed positive correlations of high intensity for the diameters studied (P < 0.01). Thus, we believed that our findings may support future reproductive studies in this species. [ABSTRACT FROM AUTHOR]

The sedimentological characteristics and fossil vertebrates studies are notoriously increasing in the earth sciences field, as a way to infer reliable information about the biota and the paleoenvironmental and paleoclimatic context. In this framework, we establish relationships among the flora, fauna and the depositional environment during the Late Miocene in Northwestern Argentina. The sedimentology features are described in detail from sediments collected in the Quebrada Salta section, and the Xenarthra Cingulata Kraglievichia paranensis (Pampatheriidae) and Cranithlastus xibiensis (Glyptodontidae) are recorded for the first time in the Palo Pintado Formation (Salta Province, Argentina). The sedimentary observations support the presence of a sinuous sandy-gravel fluvial system with swamps and lacustrine, under a wet tropical climate. In this environment, the vertebrates here described would have inhabited open zones close to these freshwater bodies, predominated by xeric vegetation, mainly represented by grasses and sedges with scarce arboreous elements. [ABSTRACT FROM AUTHOR]

There are a few studies on diseases of anteaters, but reports on reproductive lesions and neoplasms of these animals are scarce. This is the first report of a case of metastatic Sertoli cell tumour in a giant anteater (Myrmecophaga tridactyla). The animal had renal lesions associated with impaired renal function as indicated by serum biochemistry. Histopathological and immunohistochemical examinations provided a conclusive diagnosis of Sertoli cell tumour with metastasis to the liver, kidneys and lymph nodes. [ABSTRACT FROM AUTHOR]

Peltephilidae (Xenarthra, Cingulata) is an ancient lineage of medium–large‐sized 'armadillos' from South America, characterized by chisel‐shaped molariforms, a U‐shaped dental arcade, and cephalic osteoderms modified into hornlike structures. Although the biochron of the group extends from the early Eocene to the Late Miocene, the most abundant and complete records come from the Early Miocene of Patagonia. Remains from the Late Miocene are very scarce, and the last records of the group are from the Chasicoan Stage (Tortonian). The only taxon known from this interval is Epipeltephilus kanti from the Arroyo Chasicó Formation (9.23 ± 0.09 Ma; Buenos Aires Province, Argentina), a species previously represented only by a few isolated osteoderms. Here we report new remains assigned to E. kanti from the Late Miocene of Loma de Las Tapias Formation (c. 9.0–7.8 Ma; San Juan Province, Argentina), including a hemimandible and several fixed and mobile osteoderms. These new specimens constitute the youngest record of Peltephilidae. The inclusion of E. kanti within Epipeltephilus and the monophyly of the genera Peltephilus and Epipeltephilus are corroborated for the first time through a cladistic analysis. The decline and eventual disappearance of this 'armadillo' group in the Late Miocene is chronologically coincident with the replacement of subtropical/tropical environments by more open and arid ones and with the proliferation of other large armadillos such as Vetelia, Macrochorobates, and Macroeuphractus. [ABSTRACT FROM AUTHOR]

New remains of a relatively plesiomorphic nothrotheriid sloth have been recovered from upper Miocene-aged deposits near the village of Achiri in the Altiplano of Bolivia. The new specimens appear allied to other middle and late Miocene remains from Argentina and Bolivia that have been assigned to the pseudo-genus 'Xyophorus'. 'Xyophorus' has not previously been recognised as a distinct genus because of the paucity of material it encompasses. The new specimens, however, include a well-preserved squamosal with attached auditory region and an isolated astragalus. These elements, which are described in detail, provide a sufficient number of distinctive characters to place the previous fossils assigned to 'Xyophorus' into a new monotypic genus. Beyond exhibiting a suite of distinctive autapomorphies which justify its formal taxonomic designation, the new taxon not only shares several synapomorphies with more derived members of Nothrotheriidae but also retains a number of plesiomorphies characteristic of basal megatherioid taxa and shows a number of intermediate features. Although the new taxon is too incomplete to justify a full phylogenetic analysis, it appears to represent a basal member of Nothrotheriidae. Resolution of the taxonomic status of the genus Xyophorus awaits a better understanding of the taxonomy of early Miocene-aged basal megatherioids. [ABSTRACT FROM AUTHOR]

Armadillos are bitten by several species of flea. Females of the genus Tunga penetrate the epidermis and when in place are fertilised by males, after which the abdomen swells enormously to form a 'neosome'. Within the penetrans group, T. perforans, makes lesions that perforate the osteoderms within the integument to form ~3 mm diameter cavities occupied by a discoid neosome. We examined these lesions in carapace material from animals which had died in the wild to see whether we could recruit evidence as to how they may be generated, either by the insect or by the host. We studied one species without such lesions, the nine‐banded armadillo Dasypus novemcinctus, and two species with, the greater hairy armadillo Chaetophractus villosus and the southern three‐banded armadillo Tolypeutes matacus, both showing the characteristic 'flea bite' holes in the external surfaces of the osteoderms. Samples were studied by three‐dimensional backscattered electron mode scanning electron microscopy and X‐ray microtomography. Both methods showed resorption pit complexes in the external surfaces of the osteoderms characteristic of those made by osteoclasts in active bone resorption. Lesions involved both the syndesmoses (sutures) between adjacent bones and the central regions of the osteoderms. Many lesions showed extensive repair by infilling with new bone. We conclude that the T. perforans neosome creates a local host response which causes bone resorption, creating the space in which it can grow. [ABSTRACT FROM AUTHOR]

Late Pleistocene sloths were widely distributed and present in a diversity of habitats in South, Central, and North America and some Caribbean Islands. Late Pleistocene sloths include 27 genera in four families Megatheriidae, Megalonychidae, Mylodontidae, and Nothrotheriidae. There is no consensus on the number of valid species. Some sloths have wide geographic distributions and are present on multiple continents while others have a much smaller distribution. Our knowledge of the paleoecology and natural history of the different sloths varies greatly depending on their relative abundance. The wide distribution of sloths and adaptations to different habitats results in several "sloth" faunas with different taxonomic compositions. These generalized faunas can be distinguished geographically as Temperate North America (five genera), Southern Mexico and Central America (five genera), Northern South America (two genera), West Coast of South America (four genera), the Andes and Altiplano (four genera), Brazilian Intertropical Region (nine genera), Pampas-Patagonia and the Caribbean Islands (Cuba, Hispaniola, and Puerto Rico, four genera). Some genera may occur in multiple regions but are represented by different species. These regions also have differences in other mammalian taxa, so the sloths are often in ecological competition with different megaherbivores or preyed on by different carnivores. [ABSTRACT FROM AUTHOR]

The giant anteater (Myrmecophaga tridactyla) is the largest of the Neotropical vermilinguans. This animal is the second mammal most frequently run over on the roads of Cerrado biome, Brazil. Therefore, it is pivotal to know the anatomy of this species to contribute to the clinical care of these animals. Thus, the aim of this study was to identify the anatomical structures in the abdominal region of the giant anteater using computed tomography (CT). For this, the tomographic analysis was performed in sequential order in the craniocaudal direction in five giant anteaters. Moreover, four giant anteater corpses were used for anatomical studies, where two corpses were sectioned in cross sections of approximately 3 cm each, and two animals were dissected to identify the abdominal organs. The association between CT and anatomical study allowed the visualization of anatomical structures found in the abdominal cavity, such as the topographic anatomy facilitates the identification of organs on CT images. The findings of this study demonstrated that CT is a good technique to provide detailed information about the anatomical structures in the abdominal cavity and that this study can be used as CT and anatomical reference for veterinarians who care for wild animals. [ABSTRACT FROM AUTHOR]

Observing behavior in wild Southern Anteaters (Tamandua tetradactyla) allows Xenarthran researchers to gain perspective on natural history of this neotropical scansorial species. We describe the use of the prehensile tail to facilitate copulation for a pair in southeastern Brazil, a novel observation for this species. This detailed account of mating behavior in lesser anteaters is one of very few in the literature and contributes to basic knowledge of this group. Highlights: We describe part of a mating behavior of a pair of free‐ranging Southern anteaters.The opportunistic observation revealed the use of the prehensile tail to facilitate mating.Detailed mating behaviors are provided for this scansorial Neotropical species of anteater. [ABSTRACT FROM AUTHOR]

ABSTRACT This research aimed to investigate the occurrence of Chlamydia sp., Morbillivirus sp., Parvovirus sp., Leishmania sp. and Alphacoronavirus sp. in captive giant anteaters. Blood and fecal samples were taken from 16 animals in institutions from the states of Minas Gerais, Bahia and Distrito Federal, which had been in captivity for at least a year. A commercial rapid chromatographic immunoassay test was used for detecting coronavirus and parvovirus antigens, in addition to antibodies against leishmaniasis, all results being negative. In the case of the test for antibodies against distemper, four (4/16; 25%) anteaters had an average titration, two (2/16; 12.5%) a low titration and ten (10/16; 62.5%) were non-reactive. Using the DOT-ELISA (dot blotting) method for detection of immunoglobulin G, only one specimen obtained a 1 : 40 titration. For the polymerase chain reaction tests for Leishmania and Chlamydia, all samples were negative.

Sloths are among the most specialized arboreal species and have evolved a suite of anatomical features that support suspensory behaviors. While sloths generally show a reduction in skeletal muscle mass, the digital flexor musculature (i.e., the muscles associated with grip strength) remains relatively large. It may therefore be inferred that grip strength represents an important selective criterion for sloths. However, no in vivo data are available to assess the validity of this hypothesis. In this study, we explore how grip force varies as a function of substrate diameter, sidedness, and between the forelimb and hindlimb in brown‐throated three‐toed sloths (Bradypus variegatus; N = 5). Grip force was measured using a custom‐built support, comprising two semi‐circular prisms, the lower half of which was instrumented with a medium‐load force plate. Maximum recorded grip forces exceeded 100% bodyweight. While no differences were observed between the fore‐ and hindlimb pairs, both substrate diameter and sidedness significantly influenced grip strength. Specifically, B. variegatus exhibited the greatest grip force potential on the left side of their body and on large‐diameter supports. Equal gripping performance between the fore‐ and hindlimbs may be attributable to previous observations in sloths of a near‐equal distribution of bodyweight support between the limbs during suspensory locomotion. Moreover, a tendency to produce the greatest grip forces on large‐diameter substrates agrees with their ecological substrate preferences. The consistent tendency for left sidedness across the individuals studied was unexpected, and future work should explore the potential ecological and anatomical correlates of such a finding. [ABSTRACT FROM AUTHOR]

The giant anteater (Myrmecophaga tridactyla) is classified as a vulnerable species but is commonly received in rescue centers and zoos. Establishing hematological and biochemical reference intervals of free-ranging animals is an important tool to assess the health of both captive and wild populations. Reference values for 53 free-ranging giant anteaters in the Cerrado savanna of Mato Grosso do Sul state were established and differences across sexes and age groups were tested. Significant differences (p ≤ 0.05) between age groups (juvenile and adult) were found for hematocrit, mean corpuscular hemoglobin concentration (MCHC), lymphocytes, monocytes, platelets, total protein, globulin, alkaline phosphatase (ALP), glutamyl transpeptidase (GGT), and phosphorus. Between sexes, the only value that showed a significant difference was platelets. The data from the present study were also compared with previous studies and a worldwide zoological databank (ZIMS). White blood cell values were higher in all the studies with free-ranging giant anteaters compared to captive individuals. The relative eosinophils reference interval from our study was the highest ever reported for the species. This paper is the first published study with biochemical values for free-ranging giant anteaters and provides important reference intervals for future giant anteater in situ and ex situ conservation initiatives and highlights the importance of more veterinary research with comparative aspects between wild and captive animals. [ABSTRACT FROM AUTHOR]

Copyright of Acta Veterinaria Brasilica is the property of Acta Veterinaria Brasilica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We present a taxonomic revision of maned sloths, subgenus Bradypus (Scaeopus), a taxon endemic to the Brazilian Atlantic Forest and currently composed of a single species, the vulnerable Bradypus torquatus. Our review is based on coalescent species delimitation analyses using mitochondrial and nuclear DNA, morphological analyses, and field observations. Our integrative approach demonstrates that two species of maned sloth can be recognized: the northern maned sloth (Bradypus torquatus Illiger, 1811) occurring in the Brazilian states of Bahia and Sergipe, and the southern maned sloth (Bradypus crinitus Gray, 1850), occurring in Rio de Janeiro and Espirito Santo states. The two species diverged in the Early Pliocene and are allopatrically distributed. We discuss the biogeographic pattern of the two maned sloth species, comparing it with other Atlantic Forest mammals. We also suggest that the conservation status of both maned sloths needs to be reassessed after this taxonomic rearrangement. [ABSTRACT FROM AUTHOR]

The sedimentological characteristics and fossil vertebrates studies are notoriously increasing in the earth sciences field, as a way to infer reliable information about the biota and the paleoenvironmental and paleoclimatic context. In this framework, we establish relationships among the flora, fauna and the depositional environment during the Late Miocene in Northwestern Argentina. The sedimentology features are described in detail from sediments collected in the Quebrada Salta section, and the Xenarthra Cingulata Kraglievichia paranensis (Pampatheriidae) and Cranithlastus xibiensis (Glyptodontidae) are recorded for the first time in the Palo Pintado Formation (Salta Province, Argentina). The sedimentary observations support the presence of a sinuous sandy-gravel fluvial system with swamps and lacustrine, under a wet tropical climate. In this environment, the vertebrates here described would have inhabited open zones close to these freshwater bodies, predominated by xeric vegetation, mainly represented by grasses and sedges with scarce arboreous elements.

Bradypus variegatus has unique anatomical characteristics, and many of its vascular and digestive tract aspects have yet to be clearly understood. This lack of information makes clinical diagnoses and surgical procedures difficult. The aim of this study was to evaluate the anatomical aspects of frozen and glycerinated corpses of B. variegatus using computed tomography (CT), emphasizing vascular and digestive contrast studies. Nine corpses that died during routine hospital were examined via CT in the supine position with scanning in the craniocaudal direction. In frozen cadavers, the contrast was injected into a cephalic vein after thawing and, subsequently, was administered orally. In addition to bone structures, CT allowed the identification of organs, soft tissues, and vascular structures in specimens. Visualization of soft tissues was better after contrast been administered intravenously and orally, even without active vascularization. Furthermore, the surfaces of the organs were highlighted by the glycerination method. With this technique, it was possible to describe part of the vascularization of the brachial, cervical, thoracic, and abdominal regions, in addition to highlighting the esophagus and part of the stomach. CT can be another tool for the evaluation of B. variegatus cadavers by anatomists or pathologists, contributing to the identification of anatomical structures.

Using the stomach contents of 24 wild road-killed giant anteaters as proxies for their diet, we found that estimated wild diets were much lower in calcium (about ten times less) and phosphorus compared with manufactured feeds commonly fed to anteaters under human care. Indicators of soil consumption, such as ash, iron, and manganese were higher in the stomach contents than in either wild termites or manufactured diets, likely due to high levels of soil ingestion during feeding in the wild. Protein and fat levels in insects, stomach contents, and commercial diets all met carnivore recommendations. Both giant anteaters and tamanduas in managed care often develop hypercalcemia, perhaps because these taxa have an enhanced ability to retain calcium allowing them to survive on such low calcium diets. Results from this study indicate that, for anteaters in managed care, it is important to keep dietary calcium and vitamins D and K within recommended levels to prevent nutritional diseases such as hyper- and hypocalcemia and vitamin K deficiency.

This study aimed to describe the brain base arteries of the Myrmecophaga tridactyla using ten cadavers of adults from this species, including five male and five female specimens. The arterial vascular bed was perfused via the thoracic aorta with a dyed natural latex solution, and the animals were fixed and preserved with a 10% formaldehyde buffered solution. The encephala were removed, and their vessels dissected. Basilar artery formation occurred by anastomosis of the thick ventral spinal artery with vertebral arteries. The basilar artery formed two arterial islands and gave bulbar and pontine branches, and cranial, middle, and caudal cerebellar arteries and ended by forking into its terminal branches, the caudal communicating arteries. The blood supply of the encephalon derived solely from the vertebrobasilar system, and the arterial circle of the brain was closed caudally and rostrally. The absence of participation of internal carotid arteries in encephalon irrigation, the island formations by the basilar artery, and the fusiform shape of the arterial circle of the brain are peculiar characteristics of the vascular anatomy of the brain base of M. tridactyla. [ABSTRACT FROM AUTHOR]

Climate change and habitat loss have been identified as the main causes of species extinction. Forest regeneration and protected areas are essential to buffer climate change impacts and to ensure quality habitats for threatened species. We assessed the current and future environmental suitability for the maned sloth, Bradypus torquatus , under both future climate and forest restoration scenarios, using ecological niche modeling. We compared environmental suitability for two Evolutionarily Significant Units (ESUnorth and ESUsouth) using two climate change scenarios for 2070, and three potential forest regeneration scenarios. Likewise, we evaluated the protection degree of the suitable areas resulting from the models, according to Brazilian law: PA—Protected Areas; PPA—Permanent Protection Areas (environmentally sensitive areas in private properties); and LR—Legal Reserves (natural vegetation areas in private properties). Finally, we calculated the deficit of PPA and LR in each ESU, considering the current forest cover. Forest regeneration might mitigate the deleterious effects of climate change by maintaining and increasing environmental suitability in future scenarios. The ESUnorth contains more suitable areas (21,570 km²) than the ESUsouth (12,386 km²), with an increase in all future scenarios (up to 45,648 km² of new suitable areas), while ESUsouth might have a significant decrease (up to 7,546 km² less). Suitable areas are mostly unprotected (ESUnorth—65.5% and ESUsouth—58.3%). Therefore, PPA and PA can maintain only a small portion of current and future suitable areas. Both ESUs present a high deficit of PPA and LR, highlighting the necessity to act in the recovery of these areas to accomplish a large-scale restoration, mitigate climate change effects, and achieve, at least, a minimum forested area to safeguard the species. Notwithstanding, a long-term conservation of B. torquatus will benefit from forest regeneration besides those minimum requirements, allied to the protection of forest areas. [ABSTRACT FROM AUTHOR]

The cervicornis species group of Taumacera Thunberg, 1814, endemic to Sri Lanka, is revised. Seven species, including three species new to science, are recognized: Taumacera cervicornis (Baly, 1861), T. lewisi (Jacoby, 1887), T. mirabilis (Jacoby, 1887), T. unicolor (Jacoby, 1887), T. adamskii sp. nov., T. maskeliya sp. nov., and T. sigiriya sp. nov. The representatives of this group are characterized by pectinate antennomeres and by 12-segmented antennae. Antennomeres XI and XII are firmly fused in most species, separated by a distinct suture; however, in T. cervicornis, antennomere XII appears to be moveable. Color photographs of habitus, body details, and aedeagi as well as female genitalia are presented. [ABSTRACT FROM AUTHOR]

Previous studies have suggested species- and individual-specific morphologies of cephalic shields of three-banded armadillos (Tolypeutes spp.). Central cephalic scutes would form discrete lines; the first two posterior lines being composed of one scute (1-1) in Tolypeutes matacus and one followed by two scutes (1-2) in Tolypeutes tricinctus. However, conclusions were limited to a few specimens and the T. tricinctus pattern was later observed in T. matacus. Here, we assessed the potential use of the morphology of the cephalic shield as a species diagnostic trait and individual natural mark in three-banded armadillos by quantifying their patterns in museum (n = 71) and field (n = 82) specimens. In total, 93% of T. matacus and 88% of T. tricinctus specimens presented the respective species' character states. All specimens could be individualized based on a visual comparison of their cephalic shields. Our findings show that the morphology of cephalic shields is a species diagnostic trait and an individual natural mark in three-banded armadillos. Therefore, we add an accessible character for species diagnosis in Tolypeutes and provide an alternative to artificial marks that presents virtually no associated cost and damage to animal welfare and can be reliably applied in ecological and participatory science projects. [ABSTRACT FROM AUTHOR]

Cyclopes didactylus , the smallest of all anteaters, inhabits Amazonian and Atlantic forests with an apparently disjunct distribution. Yet, phylogeography reveals historical connections through the forests of the Northeast Region of Brazil. Its populations in this region are classified by the Red List of Threatened Species as Data Deficient and with a trend towards decline. However, Northeast Brazil has a large sampling gap, and the potential distribution of this species has yet to be evaluated. We investigated the potential distribution of C. didactylus to evaluate the hypothesis of a disjunct distribution between Amazonian and Atlantic forests and estimate the amount of protected area in its predicted distribution. We generated a Maxent distribution model using occurrence records, according to the new taxonomic revision of Cyclopes , and selected current bioclimatic variables to evaluate the continuity of the predicted distribution of the species in Northeast Brazil. We also performed past projections to assess historical connections and overlapped maps of protected areas onto their current distribution. Although its distribution is probably disjunct, at least one as-yet-unknown population may be present in the forests of Northeast Brazil, an area poorly protected. The results are useful for targeting field efforts in this under-sampled region. [ABSTRACT FROM AUTHOR]