Search

Copyright of Avian Diseases is the property of American Association of Avian Pathologists, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The prokaryotic expression and immunogenicity of goose tembusu virus (GTMUV) E protein domain I were identified in order to lay the foundation for further researches on biological characteristics and immunological function of E protein domain I in GTMUV. [Method] The encoding gene(EI% of GTMUV E protein domain I was artificially synthesised and was inserted into vector pGEX-4t-1 with GST tag. Then the recombinant plasmid was transformed into Escherichia coli. Through induced expression, the fusion protein was obtained and its immunogenicity was identified by using Western blotting. [Result] The obtained gene fragment E1 was 411 bp in length. Through inserting into vector pGEX-4t-1, the recombinant expression plasmid pGEX-4t-1-EI was constructed successfully. When positive recombinant strain was induced with 1 mmol/L IPTG, the expression quantity of fusion protein reached the peak after 5 hours. The fusion protein existed as inclusion body in E. coli, and the molecular weight was about 41.0 kD. The identification result of Western blotting showed that fusion protein could react specifically with GST McAb and E protein positive serum to detect out target bands. [Conclusion] The E protein domain I of GTMUV can be successfully expressed in E. coli, and the obtained fusion protein has good immunogenicity. Therefore it can be used for research & development of GTMUV serology detection kit. [ABSTRACT FROM AUTHOR]