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184 results on '"Xi, Jinghui"'

9. TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

Author

Zhang Xinxin, Yang Shuang, Zhang Xunming, Wang Shang, Zhang Juhong, and Xi Jinghui

Subjects
insect proteomics, Lissorhoptrus oryzophilus, overwintering, DEP, TMT, Physiology, QP1-981
Abstract

Adaptations to low temperature play a critical role in restricting the geographical distribution of insects. Decreasing day lengths and temperatures trigger seasonal cold adaptations in insects. These adaptions include changes in expression at the miRNA, mRNA and protein levels. The rice water weevil (RWW), Lissorhoptrus oryzophilus, introduced from the Mississippi River, is a globally invasive pest of wetland rice that can survive at the northern border of China. To investigate the changes in expression at the protein level in overwintering female RWW adults, 6-plex tandem mass tags (TMTs) were used in overwintering and summer adults. By using a proteome database available for Curculionidae, 1077 proteins were quantified, 183 of which differed significantly between the overwintering and summer samples. To further understand these differentially expressed proteins (DEPs), bioinformatics analyses such as gene ontology (GO) enrichment analyses were performed. DEPs associated with the terms binding, structural molecule activity, catalytic activity, multicellular organismal process, extracellular region, chitin binding, metabolic process, intracellular part and organic cyclic compound binding were altered by selection during winter. The changes in the expression of these proteins suggest that the proteins are important for RWW survival in winter.

Published
2020
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15. Response characteristics of highland barley (

Author

Qu, Yan, primary, Bao, Guozhang, additional, Pan, Xinyu, additional, Bao, Lan, additional, Guo, Jiancai, additional, Xi, Jinghui, additional, Zhang, Xin, additional, Yang, Yinan, additional, Zhao, Hongwei, additional, and Li, Guomei, additional

Published
2022
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22. Identification of Candidate Carboxylesterases Associated With Odorant Degradation in Holotrichia parallela Antennae Based on Transcriptome Analysis

Author

Yi, Jiankun, primary, Wang, Shang, additional, Wang, Zhun, additional, Wang, Xiao, additional, Li, Gongfeng, additional, Zhang, Xinxin, additional, Pan, Yu, additional, Zhao, Shiwen, additional, Zhang, Juhong, additional, Zhou, Jing-Jiang, additional, Wang, Jun, additional, and Xi, Jinghui, additional

Published
2021
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25. Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus.

Author

Pan, Yu, Zhang, Xinxin, Wang, Zhun, Qi, Lizhong, Zhang, Xinsheng, Zhang, Juhong, and Xi, Jinghui

Subjects
ODORANT-binding proteins, CHEMOSENSORY proteins, MEMBRANE proteins, SENSORY neurons, RICE weevil
Abstract

The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future. [ABSTRACT FROM AUTHOR]

Published
2022
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26. Odorant binding proteins promote flight activity in the migratory insect, Helicoverpa armigera

Author

Wang, Shang, Minter, Melissa, Homem, Rafael A, Michaelson, Louise V, Venthur, Herbet, Lim, Ka S, Withers, Amy, Xi, Jinghui, Jones, Chris, and Zhou, Jing-Jiang

Subjects
qx_560, wl_700, fungi
Abstract

Migratory insects are capable of actively sustaining powered flight for several hours. This extraordinary phenomenon requires a highly efficient transport system to cope with the energetic demands placed on the flight muscles. Here, we provide evidence that the role of the hydrophobic ligand binding of odorant binding proteins (OBPs) extends beyond their typical function in the olfactory system to support insect flight activity via lipid-interactions. Transcriptomic and candidate gene analyses show that two phylogenetically clustered OBPs (OBP3/OBP6) are consistently over-expressed in adult moths of the migrant Old-World bollworm, Helicoverpa armigera, displaying sustained flight performance in flight activity bioassays. Tissue-specific over-expression of OBP6 was observed in the antennae, head and thorax in long-fliers of H. armigera. Transgenic Drosophila flies over-expressing a H. armigera transcript of OBP6 (HarmOBP6) in the flight muscle attained higher flight speeds on a modified tethered flight system. Quantification of lipid molecules using mass-spectrometry showed a depletion of triacylglyerol and phospholipids in flown moths. Protein homology models built from the crystal structure of a fatty acid carrier protein identified the binding site of OBP3 and OBP6 for hydrophobic ligand binding with both proteins exhibiting a stronger average binding affinity with TAG and phospholipids compared with other groups of ligands. We propose that HarmOBP3 and HarmOBP6 contribute to the flight capacity of a globally invasive and highly migratory noctuid moth, and in doing so, extend the function of this group of proteins beyond their typical role as chemosensory proteins in insects.

Published
2020

40. Evaluation of extraction procedures for 2-DE analysis of aphid proteins

Author

An Shaoli, Zhang Hua, Xi Jinghui, Fang Kui, Li Kebin, Pan Yiou, Wang Tao, Sun Yu, and Yang Xun

Subjects
chemistry.chemical_compound, Chromatography, chemistry, Precipitation (chemistry), Extraction (chemistry), Protein purification, Acetone, Phenol extraction, Filtration and Separation, Sample preparation, Proteomics, Ammonium acetate, Analytical Chemistry
Abstract

Protein sample preparation is a crucial step in a 2-DE proteomics approach. In order to establish a routine protocol for the application of proteomics analysis to aphids, this study focuses on the specific protein extraction problems in insect tissues and evaluates four methods to bypass them. The approaches of phenol extraction methanol/ammonium acetate precipitation (PA), TCA/acetone precipitation, PEG precipitation, and no precipitation were evaluated for proteins isolation and purification from apterous adult aphids, Sitobion avenae. For 2-DE, the PA protocol was optimal, resulting in good IEF and clear spots. PA method yielded the greatest amount of protein and displayed most protein spots in 2-DE gels, as compared with the TCA/acetone precipitation, PEG precipitation and no precipitation protocols. Analysis of protein yield, image quality and spot numbers demonstrate that the TCA/acetone precipitation protocol is a reproducible and reliable method for extracting proteins from aphids. The PEG precipitation approach is a newly developed protein extraction protocol for aphids, from which more unique protein spots can be detected, especially for detection of acid proteins. These protocols are expected to be applicable to other insects or could be of interest to laboratories involved in insect proteomics, despite the amounts and types of interfering compounds vary considerably in different insects.

Published
2013

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