10 results on '"Xia GD"'
Search Results
2. Radical Phosphorylation of Aliphatic C-H Bonds via Iron Photocatalysis.
- Author
-
Xia GD, Liu ZK, Zhao YL, Jia FC, and Hu XQ
- Abstract
The synthesis of tertiary phosphines(III) has been a long-standing challenge in synthetic chemistry because of inevitable issues including harsh conditions, sensitive organometallic reagents, and prefunctionalized substrates in traditional synthesis. Herein, we report a strategically novel C(sp
3 )-H bond phosphorylation that enables the assembly of structurally diverse tertiary phosphines(III) from industrial phosphine(III) sources under mild photocatalytic conditions. The merger of ligand-to-metal charge transfer (LMCT) of FeCl3 with the hydrogen atom-transfer (HAT) process is the key for the generation of alkyl radicals from hydrocarbons. Strikingly, this catalytic system can be successfully applied for the polymerization of electron-deficient alkenes.- Published
- 2023
- Full Text
- View/download PDF
3. Deoxygenative gem -difluorovinylation of aliphatic alcohols.
- Author
-
Xia GD, He YY, Zhang J, Liu ZK, Gao Y, and Hu XQ
- Subjects
- Catalysis, Molecular Structure, Alcohols, Alkenes
- Abstract
An unprecedented deoxygenative gem -difluorovinylation of aliphatic alcohols using α-trifluoromethyl alkenes is achieved under photocatalytic conditions. Inexpensive Ph
3 P acts as an efficient O-atom transfer reagent to facilitate the deoxygenation of alcohols for the generation of reactive alkyl radical species. Remarkable features of this reaction include mild conditions, simple operation and broad scope. The synthetic utility of this reaction was validated by the success of two-step one-pot reactions, scale-up synthesis and chemoselective monodeoxygenation of diols.- Published
- 2022
- Full Text
- View/download PDF
4. [Effect of different fertilization treatments on yield and secondary metabolites of Codonopsis pilosula].
- Author
-
Hu JD, Mao G, Zhang ZW, Ma CD, Liang ZS, Xia GD, and Dong JE
- Subjects
- Biomass, Codonopsis growth & development, Nitrogen, Plant Roots growth & development, Plants, Medicinal growth & development, Secondary Metabolism, Codonopsis chemistry, Fertilizers, Phytochemicals analysis, Plants, Medicinal chemistry
- Abstract
The research studies the effect of different fertilization treatments on yield and accumulation of secondary metabolites of Codonopsis pilosula by using single factor randomized block design, in order to ensure reasonable harvesting time and fertilization ratio, and provide the basis for standardized cultivation of C. pilosula. According to the clustering results, the nitrogen fertilizer benefitted for the improvement of root diameter and biomass of C. pilosula. The phosphate fertilizer could promote the content of C. pilosula polysaccharide. The organic fertilizers could increase the content of lobetyolin. With the time going on, C. pilosula's yield, polysaccharide and ehanol-soluble extracts increased while the content of lobetyolin decreased. According to various factors, October is a more reasonable harvest period. Organic fertilizers are more helpful to the yield and accumulation of secondary metabolites of C. pilosula., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
5. Microencapsulation of recombinant adenovirus within poly-DL-lactide-poly(ethylene glycol) microspheres for enhanced gene transfection efficiency and inhibitory effects on hepatocellular carcinoma cells in vitro.
- Author
-
Xia D, Feng LB, Wu XL, Xia GD, and Xu L
- Subjects
- Adenoviridae chemistry, Animals, Cell Movement, Cell Proliferation, Collagen chemistry, Drug Combinations, Drug Compounding methods, Gene Expression, Genetic Vectors, Hep G2 Cells, Humans, Laminin chemistry, Microspheres, Particle Size, Proteoglycans chemistry, Rats, Tissue Inhibitor of Metalloproteinase-1 metabolism, Transgenes, Adenoviridae genetics, Genetic Therapy methods, Lactates chemistry, Polyethylene Glycols chemistry, Tissue Inhibitor of Metalloproteinase-1 genetics, Transfection methods
- Abstract
When gene therapy is performed for the treatment of malignant tumors, gene transfer efficiency and selectivity are highly important. Polymer vehicle microspheres are a novel type of therapy, which have been developed rapidly in recent years and are able to control drug release, prolong the biological half-life of drugs, decrease side effects and achieve targeted delivery. The present study was designed to construct a polymer microsphere-encapsulated recombinant adenovirus with human tissue inhibitors of the matrix metalloproteinase-1 (TIMP-1) gene, and to discuss its characterization for the purpose of liver cancer gene therapy. The microsphere was prepared from biodegradable poly-DL-lactide-poly(ethylene glycol) (PELA) encapsulating rAdTIMP-1, the recombinant adenovirus carrying TIMP-1, by a modified double-emulsion method. The particle morphology, diameter, virus encapsulation, loading rate and release kinetics of the rAd-microspheres were determined in vitro. Hepatocellular carcinoma (HCC) HepG2 cells were transfected with the rAd-microsphere and the efficiency of transfection was assessed by fluorescent microscopy. The production and expression of TIMP-1 was identified by gelatin zymography and western blot analysis, and the invasiveness was detected by a matrigel matrix invasion assay. The microsphere encapsulating rAdTIMP-1 was successfully constructed with a diameter of 1.965 μm, encapsulation efficiency of 60.0%, a viral load of 10.5 x 10(8)/mg, a virus release of ~60% within 120 h and a total release time of >240 h. The resultant rAd-microspheres were able to efficiently transfect HepG2 cells with the transfection efficiency enhanced by ~90%. As a result, the transfected HepG2 cells had significantly increased TIMP-1 enzyme activity and the expression of TIMP-1 was detected by western blot analysis. In addition, the proliferation and invasion ability of the HCC cells was markedly inhibited by the rAd-microspheres. The resultant rAd-microspheres, PELA-encapsulated recombinant TIMP-1 adenovirus, had enhanced transfection efficiency and were able to markedly inhibit the in vitro biological behavior of HepG2 cells. This provides an experimental basis for this polymer application and may pave the way for prospective in vivo clinical trials and further comprehensive therapy for liver cancer.
- Published
- 2015
- Full Text
- View/download PDF
6. Efficacy of tandospirone in patients with irritable bowel syndrome-diarrhea and anxiety.
- Author
-
Lan L, Chen YL, Zhang H, Jia BL, Chu YJ, Wang J, Tang SX, and Xia GD
- Subjects
- Abdominal Pain drug therapy, Abdominal Pain etiology, Adult, Aged, Anti-Anxiety Agents administration & dosage, Anti-Anxiety Agents adverse effects, Anxiety diagnosis, Anxiety etiology, Anxiety psychology, Calcium Channel Blockers therapeutic use, China, Diarrhea diagnosis, Diarrhea etiology, Drug Administration Schedule, Female, Humans, Irritable Bowel Syndrome complications, Irritable Bowel Syndrome diagnosis, Irritable Bowel Syndrome psychology, Isoindoles administration & dosage, Isoindoles adverse effects, Male, Middle Aged, Morpholines therapeutic use, Pain Measurement, Piperazines administration & dosage, Piperazines adverse effects, Prospective Studies, Pyrimidines administration & dosage, Pyrimidines adverse effects, Single-Blind Method, Surveys and Questionnaires, Time Factors, Treatment Outcome, Young Adult, Anti-Anxiety Agents therapeutic use, Anxiety drug therapy, Diarrhea drug therapy, Irritable Bowel Syndrome drug therapy, Isoindoles therapeutic use, Piperazines therapeutic use, Pyrimidines therapeutic use
- Abstract
Aim: To investigate the efficacy of tandospirone in patients with irritable bowel syndrome-diarrhea (IBS-D) and anxiety in a prospective, randomized, controlled study., Methods: Two hundred patients with IBS-D and moderate anxiety were randomized to receive pinaverium and tandospirone (arm A) or pinaverium and placebo (arm B). Tandospirone or placebo was given thrice daily at a fixed dose of 10 mg and pinaverium was given thrice daily at a fixed dose of 50 mg. The duration of treatment was 8 wk. Patients were assessed for abdominal pain and diarrhea. Anxiety was evaluated using the Hamilton Rating Scale for Anxiety (HAM-A). The primary study endpoints were response rates for abdominal pain and diarrhea. The secondary study endpoints were response rates for anxiety. Adverse events were also evaluated., Results: One hundred and seventy of 200 patients (82 patients in arm A and 88 patients in arm B) completed the study. Demographic and baseline characteristics of the 200 participants were comparable in the two arms. At week 8, the overall response rate for abdominal pain and diarrhea was 52.0% for arm A and 37.0% for arm B (P < 0.05). The HAM-A score showed that the response rate was 61.0% for arm A and 21.0% for arm B (P < 0.01). The treatments were well tolerated and no significant adverse events were reported., Conclusion: Tandospirone is effective and can be combined with pinaverium in IBS-D patients with anxiety.
- Published
- 2014
- Full Text
- View/download PDF
7. [Expression of calcium-activated potassium channel in rat hepatic stellate cells].
- Author
-
Zhou X, Shao ZY, Li CP, Deng MM, Xia GD, and Li ML
- Subjects
- Animals, Cells, Cultured, Male, Patch-Clamp Techniques, Rats, Rats, Wistar, Hepatic Stellate Cells metabolism, Potassium Channels, Calcium-Activated metabolism
- Published
- 2008
8. Significance of platelet activating factor receptor expression in pancreatic tissues of rats with severe acute pancreatitis and effects of BN52021.
- Author
-
Xia SH, Hu CX, Zhao ZL, Xia GD, and Di Y
- Subjects
- Acute Disease, Amylases blood, Animals, Fibrinolytic Agents therapeutic use, Ginkgolides therapeutic use, Lactones therapeutic use, Male, Pancreatitis drug therapy, Pancreatitis pathology, Platelet Activating Factor genetics, Platelet Activating Factor metabolism, Platelet Membrane Glycoproteins genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Random Allocation, Rats, Rats, Wistar, Receptors, G-Protein-Coupled genetics, Fibrinolytic Agents pharmacology, Gene Expression Regulation drug effects, Ginkgolides pharmacology, Lactones pharmacology, Pancreatitis metabolism, Platelet Membrane Glycoproteins metabolism, Receptors, G-Protein-Coupled metabolism
- Abstract
Aim: To investigate the dynamic changes and significance of platelet activating factor receptor (PAF-R) mRNA and protein in pancreatic tissues of rats with severe acute pancreatitis (SAP) and effects of BN52021 (Ginkgolide B)., Methods: Wistar male rats were randomly assigned to the negative control group (NC group), SAP model group (SAP group), and BN52051-remedy group (BN group), and each of the groups was divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h) (n = 10 in each). PT-PCR and Western blot methods were used to detect PAF-RmRNA and protein expression in pancreatic tissues of rats respectively. Pathological examination of pancreatic tissues was performed and the serum amylase change was detected., Results: Serum amylase and pathological results showed the that SAP model was successfully prepared, BN52021 was able to decrease serum amylase, and the pathological ratings in BN group at 3 h, 6 h, and 12 h significantly decreased compared with those in the SAP group (8.85 +/- 0.39 vs 5.95 +/- 0.19, 9.15 +/- 0.55 vs 5.55 +/- 0.36, 10.10 +/- 0.65 vs 6.72 +/- 0.30, P < 0.05). The result of PAF-mRNA showed dynamic changes in SAP and BN groups, which increased gradually in early stage, reached a peak at 3 h (0.71 +/- 0.14 vs 0.54 +/- 0.14, 0.69 +/- 0.13 vs 0.59 +/- 0.04, P < 0.05), and decreased gradually later. There were significant differences at each time point except 1 h and 2 h, when compared with those in the NC group (0.71 +/- 0.14 or 0.69 +/- 0.13 vs 0.47 +/- 0.10, 0.38 +/- 0.08 or 0.59 +/- 0.04 vs 0.47 +/- 0.09, 0.25 +/- 0.07 or 0.29 +/- 0.05 vs 0.46 +/- 0.10, 0.20 +/- 0.06 or 0.20 +/- 0.04 vs 0.43 +/- 0.09, P < 0.05), whereas there was no significant difference between BN and SAP groups at each time point. The result of PAF-R protein showed that the change of PAF-R protein in the SAP group and the BN group was consistent with that of PAF-R mRNA. There were significant differences at each time point except 1 h, when compared with those in the NC group (0.90 +/- 0.02 or 0.80 +/- 0.05 vs 0.48 +/- 0.02, 1.69 +/- 0.06 or 1.58 +/- 0.02 vs 0.48 +/- 0.03, 1.12 +/-0.10 or 0.98 +/- 0.03 vs 0.49 +/- 0.09, 1.04 +/- 0.14 or 0.87 +/- 0.02 vs 0.52 +/- 0.08, 0.97 +/- 0.16 or 0.90 +/- 0.05 vs 0.49 +/- 0.10, P < 0.05), whereas there was no significant difference between the BN group and the SAP group., Conclusion: PAF-R plays an important role in occurrence and development of SAP. BN52021 exerts biological effects through competitively inhibiting the binding of increased both PAF and PAF-R expression rather than through decreasing PAF-R expression in pancreatic tissues.
- Published
- 2007
- Full Text
- View/download PDF
9. [Brain abscess (author's transl)].
- Author
-
Xia GD
- Subjects
- Adolescent, Adult, Brain Abscess surgery, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Brain Abscess etiology
- Published
- 1979
10. [Diagnosis and treatment of delayed intracranial hematoma].
- Author
-
Xia GD
- Subjects
- Adolescent, Adult, Aged, Brain Injuries complications, Cerebral Hemorrhage surgery, Child, Female, Hematoma surgery, Hematoma, Epidural, Cranial diagnostic imaging, Hematoma, Epidural, Cranial surgery, Humans, Male, Middle Aged, Tomography, X-Ray Computed, Cerebral Hemorrhage diagnostic imaging, Hematoma diagnostic imaging
- Abstract
20 patients of head injury with delayed intracranial hematoma were diagnosed by CT scan. There are 15 men and 5 women, and ages ranged from 12-74 years. The location of hematomas were epidural hematoma 9, subdural 1, and intracerebral 10. The time of hematoma formation ranged from 12 hours to 21 days. Those were not found at first, but lately were diagnosed by CT scan. Early diagnosis, early treatment and prognosis are discussed also.
- Published
- 1989
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.