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In this study, three genes, PmATP synthase, PmNa+-K+-ATPase and PmToll receptor gene, were selected to study their relative expression in different tissues of Penaeus monodon. The results showed that the mRNA level of PmATP synthase gene was not significantly up-regulated under low salt stimulation (P> 0.05),while under high salinity stimulation, the transcription level of PmATP synthase mRNA reached the maximum at 16th h (P<0.05).The expression level of PmNa+-K+-ATPase mRNA increased at 4th h and 16th h, which was significantly higher than that in the beginning under low salt stimulation (P<0.05) and then decreased at 32nd h,,while under high salinity stimulation, the expression level of PmNa+-K+- ATPase mRNA reached the highest at 8th h and 32nd h. The expression level of PmToll receptor mRNA reached the highest at 4th h after low salinity stimulation (P<0.05), and then down-regulated after 16th h, while under high salinity stress, the expression level was up-regulated and reached the maximum at 8th h, which was 2.4 times of that the in the control group (P<0.05) and decreased at 16th h after stress. PmATP synthase gene was relatively high expression, while PmNa+-K+-ATPase and PmToll receptor gene expression were low, so we guess that the large amounts of ATP of the body synthesis mainly use for the growth of the body. PmNa+-K+-ATPase and PmToll receptor gene expression are relatively high, which indicate that the body osmotic pressure regulation and the body’s immune process consume a lot of energy [ABSTRACT FROM AUTHOR]

To explore the effect of concentrated dephenolic cottonseed protein (CDCP) partly instead of fish meal on the non-specific immune of Penaeus monodon, we designed a single factor test to study the four types of non-specific immunity of P. monodon hepatopancreas after acute salinity stress. A total number of 5,400 P. monodon were selected with an initial body weight of 1.76±0.15g and randomly divide into 6 groups (3 replicates per group, 900 tails per replicate). Shrimp were feed with two protein level diets (Diet A and Diet B). After 8 weeks of feeding, P. monodon were randomly selected with an average weight of 14.71±1.06g in each feed group, and subjected to acute salinity stress of 10ppt and 20ppt after temporary rearing, and the heat shock protein 70 (HSP70), Na+-K+-ATPase, cortisol and the content of alkaline phosphatase (ALP) were measured. The results of the study showed that when the CDCP partially replaced fish meal of P. monodon diet, the hepatopancreas HSP70, and the contents of Na+-K+-ATPase and cortisol were significantly higher than those of the control group. In the acute salinity change, there was no significant difference in the survival rate of P. monodon at 48h under the two salinity stresses, and the four immune indexes showed different trends. The expression level of Cortisol was more prominent than that of HSP70, which could better reflect the stress degree of P. monodon. Most of the indicators showed a trend of rising and then falling and then rising compared with the original level, and the changes of the four indicators of hepatopancreas in the Diet B group were significantly higher than those in the Diet A group at most time point of the two salinities. [ABSTRACT FROM AUTHOR]