Search

In the genomes of diploid organisms, runs of homozygosity (ROH), consecutive segments of homozygosity, are extended. ROH can be applied to evaluate the inbreeding situation of individuals without pedigree data and to detect selective signatures via ROH islands. We sequenced and analyzed data derived from the whole-genome sequencing of 97 horses, investigated the distribution of genome-wide ROH patterns, and calculated ROH-based inbreeding coefficients for 16 representative horse varieties from around the world. Our findings indicated that both ancient and recent inbreeding occurrences had varying degrees of impact on various horse breeds. However, recent inbreeding events were uncommon, particularly among indigenous horse breeds. Consequently, the ROH-based genomic inbreeding coefficient could aid in monitoring the level of inbreeding. Using the Thoroughbred population as a case study, we discovered 24 ROH islands containing 72 candidate genes associated with artificial selection traits. We found that the candidate genes in Thoroughbreds were involved in neurotransmission (CHRNA6, PRKN, and GRM1), muscle development (ADAMTS15 and QKI), positive regulation of heart rate and heart contraction (HEY2 and TRDN), regulation of insulin secretion (CACNA1S, KCNMB2, and KCNMB3), and spermatogenesis (JAM3, PACRG, and SPATA6L). Our findings provide insight into horse breed characteristics and future breeding strategies.

Water scarcity is one of the main challenges worldwide that might propose various engineering and economic issues. Petroleum industries have encountered these issues seriously, which required pretreatment facilities before reinjecting the produced water into the production wells. To assure that the treated water has no side effect on the environment, the treatment processes would perform three times by the photo-Fenton flotation method. This paper, it is aimed to calculate the treated water, required water, and saving water for chemically enhanced oil recovery methods (CEOR), hydraulic fracturing (HF), and other service facilities for completion and drilling performances. According to the results of this study, oil-well#3 has the highest water savings among all the wells with the 89% of daily water-saving, and oil-well#4 has the highest water savings among oil wells with the 75% of daily water saving. Consequently, in Sirri oilfield, the water-saving percentage is about 82% and 72%, which indicated that it is required 18%, 28% of the total water volume for implantation of HF process and CEOR methods, respectively. The total annual required water for this oilfield is 4131 MM m3/Day.

Sertoli cells play vital roles in normal spermatogenesis, and microRNAs (miRNAs) participate in regulating Sertoli cell development. However, the functions and mechanisms of action of most identified miRNAs in porcine Sertoli cells remain largely unknown. Herein, we primarily explored the regulatory roles of miR-130a in immature porcine Sertoli cells using EdU-based high-content screening assay. The results demonstrated that 27 miRNAs have potential roles in the promotion of immature porcine Sertoli cell proliferation, and miR-130a was identified as a promising candidate. miR-130a promoted cell cycle progression and cell proliferation, whereas it impeded cell apoptosis in immature porcine Sertoli cells. It also contributed to Sertoli cell proliferation and testis development in vivo. A TMT-based proteomics approach revealed that miR-130a regulated the expression of 91 proteins and multiple pathways, including the TGF-β and PI3K/AKT signaling. miR-130a did not directly target the 3'-UTR of SMAD5; however, it increased SMAD5 phosphorylation. Moreover, miR-130a enhanced TGF-β signaling by activating SMAD5 protein, and TGF-β signaling further activated the PI3K/AKT signaling pathway to promote cell proliferation and inhibit cell apoptosis in porcine immature Sertoli cells. Collectively, miR-130a promoted immature porcine Sertoli cell growth by activating SMAD5 through the TGF-β-PI3K/AKT signaling pathway. This study, therefore, provides novel insights into the effects of miR-130a on porcine spermatogenesis through the regulation of immature Sertoli cell proliferation and apoptosis.

Immature Sertoli cell proliferation determines the total number of mature Sertoli cells and further regulates normal spermatogenesis. Accumulating evidence demonstrates that microRNAs (miRNAs) play regulatory roles in immature Sertoli cell proliferation, while the functions and mechanisms of the Sertoli cells of domestic animals are poorly understood. In the present study, we aimed to investigate the roles of miR-362 in cell proliferation and apoptosis of porcine immature Sertoli cells. The results showed that miR-362 inhibition promoted the entrance of cells into the S phase and increased the expressions of cell cycle-related genes c-MYC, CNNE1, CCND1 and CDK4. Knock-down of miR-362 also promoted cell proliferation and inhibited apoptosis, which was demonstrated by the results from cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) and Annexin V-FITC/PI staining assays. The recQ-mediated genome instability protein 1 (RMI1) gene was identified as a potential target gene of miR-362 via luciferase reporter assay, and miR-362 repressed the protein expression of RMI1 in porcine immature Sertoli cells. siRNA-induced RMI1 knock-down further abolished the effects of miR-362 inhibition on porcine immature Sertoli cells. Collectively, we concluded that miR-362 knock-down promotes proliferation and inhibits apoptosis in porcine immature Sertoli cells by targeting the RMI1 gene, which indicates that miR-362 determines the fate of immature Sertoli cells.

Sertoli cells are the crucial coordinators to guarantee normal spermatogenesis and male fertility. Although circular RNAs (circRNAs) exhibit developmental-stage-specific expression in porcine testicular tissues and have been thought of as potential regulatory molecules in spermatogenesis, their functions and mechanisms of action remain largely unknown, especially in domestic animals. A novel circBTBD7 was identified from immature porcine Sertoli cells using reverse transcription PCR, Sanger sequencing, and fluorescence in situ hybridization assays. Functional assays illustrated that circBTBD7 overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis in immature porcine Sertoli cells. Mechanistically, circBTBD7 acted as a sponge for the miR-24-3p and further facilitated its target mitogen-activated protein kinase 7 (MAPK7) gene. Overexpression of miR-24-3p impeded cell proliferation and induced cell apoptosis, which further attenuated the effects of circBTBD7 overexpression. siRNA-induced MAPK7 deficiency resulted in a similar effect to miR-24-3p overexpression, and further offset the effects of miR-24-3p inhibition. Both miR-24-3p overexpression and MAPK7 knockdown upregulated the p38 phosphorylation activity. The SB202190 induced the inhibition of p38 MAPK pathway and caused an opposite effect to that of miR-24-3p overexpression and MAPK7 knockdown. Collectively, circBTBD7 promotes immature porcine Sertoli cell growth through modulating the miR-24-3p/MAPK7 axis to inactivate the p38 MAPK signaling pathway. This study expanded our knowledge of noncoding RNAs in porcine normal spermatogenesis through deciding the fate of Sertoli cells.

MicroRNAs (miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically (P0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted (P

Sertoli cells are central and essential coordinators of spermatogenesis. Accumulating evidence has demonstrated that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and the regulatory mechanisms of miRNAs in Sertoli cells of domestic animals remain largely unknown. Here we report that miR-222 overexpression repressed cell cycle progression and proliferation and promoted the apoptosis of immature porcine Sertoli cells, whereas miR-222 inhibition resulted in the opposite result. miR-222 directly targeted the 3′-UTR of the GRB10 gene and inhibited its mRNA abundance. An siRNA-induced GRB10 knockdown showed similar effects as did miR-222 overexpression on cell proliferation and apoptosis and further attenuated the role of miR-222 inhibition. Furthermore, both miR-222 overexpression and GRB10 inhibition repressed the phosphorylation of PI3K and AKT, the key elements of the PI3K/AKT signaling pathway, whereas GRB10 inhibition offsets the effects of the miR-222 knockdown. Overall, we concluded that miR-222 suppresses immature porcine Sertoli cell growth by targeting the GRB10 gene through inactivation of the PI3K/AKT signaling pathway. This study provides novel insights into the epigenetic regulation of porcine spermatogenesis by determining the fate of Sertoli cells.

The quantity of Sertoli cells in the adult testis decides the daily gamete formation, and accumulating evidence indicates that epigenetic factors regulate the proliferation of Sertoli cells. Research on the function and regulatory mechanism of microRNAs (miRNAs) in Sertoli cells has not been comprehensive yet, especially on domestic animals. In this article, we report that miR-126 controls the proliferation and apoptosis of immature porcine Sertoli cells based on previous studies. Our results confirmed that miR-126 elevation promotes cell cycle progression, cell proliferation and represses cell apoptosis, on the contrary, the inhibitory effects of miR-126 result in the opposite. The phosphoinositide-3-kinase regulatory subunit 2 (PIK3R2) gene, a member of the PI3K family, was verified as a direct target of miR-126 using the dual-luciferase reporter analysis. miR-126 negatively regulated the mRNA and protein expression level of PIK3R2 in immature porcine Sertoli cells. siRNA-induced PIK3R2 inhibition caused similar effects as miR-126 overexpression and eliminated the influences of miR-126 knockdown in immature porcine Sertoli cells. In addition, both miR-126 overexpression and PIK3R2 inhibition elevated the phosphorylation of PI3K and AKT, whereas the miR-126 knockdown demonstrated the contrary result. In short, miR-126 controls the proliferation and apoptosis of immature porcine Sertoli cells by targeting the PIK3R2 gene through the PI3K/AKT signaling pathway. The research supplies a theoretical and practical foundation for exploring the functional parts of miR-126 in swine sperm by defining the destiny of immature Sertoli cells.

Sertoli cells are indispensable for normal spermatogenesis, and increasing evidence has shown that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and regulatory mechanisms of miRNAs in Sertoli cells of domestic animals have not been fully investigated. In the present study, we mainly investigated the regulatory roles of miR-499 in immature porcine Sertoli cells. The results showed that miR-499 was mainly located in the basement section of seminiferous tubules of prepubertal porcine testicular tissue. Overexpression of miR-499 promoted cell proliferation and inhibited apoptosis, whereas miR-499 inhibition resulted in the opposite effect. The PTEN gene was directly targeted by miR-499, and the expression of mRNA and protein was also negatively regulated by miR-499 in immature porcine Sertoli cells. siRNA-induced PTEN knockdown resulted in a similar effect as an overexpression of miR-499 and abolished the effects of miR-499 inhibition on immature porcine Sertoli cells. Moreover, both miR-499 overexpression and the PTEN knockdown activated the PI3K/AKT signaling pathway, whereas inhibition of the PI3K/AKT signaling pathway caused immature porcine Sertoli cell apoptosis and inhibited cell proliferation. Overall, miR-499 promotes proliferation and inhibits apoptosis in immature porcine Sertoli cells through the PI3K/AKT pathway by targeting the PTEN gene. This study provides novel insights into the effects of miR-499 in spermatogenesis through the regulation of immature Sertoli cell proliferation and apoptosis.

The Alqal Copper-lead-zinc deposit isl ocated in the Tarim plate of the Xinjiang. It has undergone multiple stages of tectonic mineralization and is an important deposit in the Kuslafu metallogenic belt of the West Kunlun area. Based on the analysis of the metallogenic geological conditions of the deposit, The composition of fluid inclusions in mining area was studied, The characteristics of metallogenic fluid are discussed. The results show that the uniform temperature of gas-liquid two-phase fluid inclusions in the Alqal deposit is between 145°C and 267°C, the average uniform temperature is 191°C; the salinity is 0.71% to 14.87%, and the average salinity is 6.33%. The estimated metallogenic pressure is about 135MPa, and the capture temperature is concentrated around 320°C. The ore-forming fluid has undergone multi-stage superimposed transformation, which is inconsistent with the characteristics of the typical MVT deposit, and it is a sedimentary transformation type deposit.

In order to solve such problems as ground subsidence, water and mud bursting and shield machine foundering in subway construction which are caused by water-rich karst geological conditions, complete technological process is established to detect and treat karst in urban areas. The occurrence of natural electric field frequency selection method and groundwater determine groundwater occurrence vector that karst and fault fracture zone, and make use of the grouting technology of karst fracture with the treatment. The construction of Yingwanzhen Stationon Changsha Metro Line No. 4 provesthe favorable effect of the detection technology and treatment measures, which provides experience and reference for the construction of Changsha subway and underground engineeringin other cities with water-rich karst areas.

Substorms generally inject tens to hundreds of keV electrons, but intense substorm electric fields have been shown to inject MeV electrons as well. An intriguing question is whether such MeVelectron injections can populate the outer radiation belt. Here we present observations of a substorm injection of MeV electrons into the inner magnetosphere. In the premidnight sector at L ∼ 5.5, Van Allen Probes (Radiation Belt Storm Probes)‐A observed a large dipolarization electric field (50 mV/m) over ∼40 s and a dispersionless injection of electrons up to ∼3 MeV. Pitch angle observations indicated betatron acceleration of MeV electrons at the dipolarization front. Corresponding signals of MeV electron injection were observed at LANL‐GEO, THEMIS‐D, and GOES at geosynchronous altitude. Through a series of dipolarizations, the injections increased the MeV electron phase space density by 1 order of magnitude in less than 3 h in the outer radiation belt (L > 4.8). Our observations provide evidence that deep injections can supply significant MeV electrons., Key Points Unambiguous evidence of deep injections of MeV electrons from multispacecraftExtremely large electric fields (50 mV/m) associated with the dipolarizationStrong dipolarizations may supply significant MeV electrons to radiation belts

We present the first observations of large-amplitude electrostatic ion cyclotron (EIC) waves near the Earth's dayside magnetopause at MLT of ∼14 using data from Time History of Events and Macroscale Interactions during Substorms (THEMIS) satellites. The EIC waves were identified in a boundary layer in the magnetosphere adjacent to the magnetopause where reconnection was occurring. The EIC wave power was primarily at 2fcH (where fcH is the hydrogen cyclotron frequency) and simultaneously observed with perpendicular ion heating. The EIC waves had electric field amplitudes as large as 30 mV/m peak to peak with significant power both perpendicular and parallel to the magnetic field. These amplitudes were greater than those of previously observed ion cyclotron harmonics at the nightside magnetopause. The EIC waves occurred during an interval of enhancements in the quasi-static electric field and fluctuations in the background magnetic field, plasma density, and temperatures. The observations indicate that a plasma density gradient is a possible source of free energy for the EIC waves. The observed flow shears are not large enough to drive the waves. Whistler mode waves were identified near the EIC wave region but closer to the magnetopause in a region with slightly higher ion and electron temperatures.

We present Cluster spacecraft observations of large-amplitude (δ|B|/|B|∼1) fast-mode magnetosonic waves in the heliospheric current sheet (HCS). The crossings of the HCS and the associated heliospheric plasma sheet (HPS) are encountered by Cluster in the near-Earth solar wind and by ACE upstream of Earth. Multiple current layers are detected in correspondence with small-scale discontinuities in the regime of open magnetic field lines within the HCS. Fast magnetosonic waves are observed at one current layer, accompanying the phase-steeped edge of a large-amplitude transverse Alfven wave. The observed fast-mode waves are in the frequency range 0.01 Hz–0.2 Hz, characterized by a strong correlation between variations of plasma density and magnetic field strength. Analysis of ratio δE/δBindicate that the fast-mode wave packet consists of an antisunward propagating component and a larger sunward propagating component in the rest frame of the solar wind. The fast-mode waves are not observed by ACE in the upstream solar wind. The generation of the fast-mode waves may relate to the development of the phase-steeped Alfven wave and has profound effects on the evolution of the solar wind plasma.

Observation and model results accumulated in the last decade indicate that substorms can promptly inject relativistic ‘killer’ electrons (≥MeV) in addition to 10–100 keV subrelativistic populations. Using measurements from Cluster, Polar, LANL, and GOES satellites near the midnight sector, we show in two events that intense electric fields, as large as 20 mV/m, associated with substorm dipolarization are associated with injections of relativistic electrons into the outer radiation belt. Enhancements of hundreds of keV electrons at dipolarization in the magnetotail can account for the injected MeV electrons through earthward transport. These observations provide evidence that substorm electric fields inject relativistic electrons by transporting magnetotail electrons into the outer radiation belt. In these two events, injected relativistic electrons dominated the substorm timescale enhancement of MeV electrons as observed at geosynchronous orbit.

We present the first observations of large amplitude waves in a well-defined electron diffusion region at the sub-solar magnetopause using data from one THEMIS satellite. These waves identified as whistler mode waves, electrostatic solitary waves, lower hybrid waves and electrostatic electron cyclotron waves, are observed in the same 12-sec waveform capture and in association with signatures of active magnetic reconnection. The large amplitude waves in the electron diffusion region are coincident with abrupt increases in electron parallel temperature suggesting strong wave heating. The whistler mode waves which are at the electron scale and enable us to probe electron dynamics in the diffusion region were analyzed in detail. The energetic electrons (~30 keV) within the electron diffusion region have anisotropic distributions with T_{e\perp}/T_{e\parallel}>1 that may provide the free energy for the whistler mode waves. The energetic anisotropic electrons may be produced during the reconnection process. The whistler mode waves propagate away from the center of the 'X-line' along magnetic field lines, suggesting that the electron diffusion region is a possible source region of the whistler mode waves.