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DNA damage response (DDR) in eukaryotes is essential for the maintenance of genome integrity in challenging environments. The regulatory mechanisms of DDR have been well-established in yeast and humans. However, increasing evidence supports the idea that plants seem to employ different signaling pathways that remain largely unknown. Here, we report the role of MODIFIER OF SNC1, 4-ASSOCIATED COMPLEX SUBUNIT 5A (MAC5A) in DDR in Arabidopsis (Arabidopsis thaliana). Lack of MAC5A in mac5a mutants causes hypersensitive phenotypes to methyl methanesulfonate (MMS), a DNA damage inducer. Consistent with this observation, MAC5A can regulate alternative splicing of DDR genes to maintain the proper response to genotoxic stress. Interestingly, MAC5A interacts with the 26S proteasome (26SP) and is required for its proteasome activity. MAC core subunits are also involved in MMS-induced DDR. Moreover, we find that MAC5A, the MAC core subunits, and 26SP may act collaboratively to mediate high-boron-induced growth repression through DDR. Collectively, our findings uncover the crucial role of MAC in MMS-induced DDR in orchestrating growth and stress adaptation in plants.

Aluminum (Al) toxicity is a primary limiting factor for crop production in acidic soils. The WRKY transcription factors play important roles in regulating plant growth and stress resistance. In this study, we identified and characterized two WRKY transcription factors, SbWRKY22 and SbWRKY65, in sweet sorghum (Sorghum bicolor L.). Al induced the transcription of SbWRKY22 and SbWRKY65 in the root apices of sweet sorghum. These two WRKY proteins were localized in the nucleus and exhibited transcriptional activity. SbWRKY22 showed the significant transcriptional regulation of SbMATE, SbGlu1, SbSTAR1, SbSTAR2a, and SbSTAR2b, which are major known Al tolerance genes in sorghum. Interestingly, SbWRKY65 had almost no effect on the aforementioned genes, but it significantly regulated the transcription of SbWRKY22. Therefore, it is speculated that SbWRKY65 might indirectly regulate Al-tolerance genes mediated by SbWRKY22. The heterologous expression of SbWRKY22 and SbWRKY65 greatly improved the Al tolerance of transgenic plants. The enhanced Al tolerance phenotype of transgenic plants is associated with reduced callose deposition in their roots. These findings suggest the existence of SbWRKY22- and SbWRKY65-mediated Al tolerance regulation pathways in sweet sorghum. This study extends our understanding of the complex regulatory mechanisms of WRKY transcription factors in response to Al toxicity.

A novel input/output feedback linearization control method by utilizing nonlinear disturbance observer (NDOB) is proposed for a quadruple-tank liquid level (QTLL) system in this paper. Firstly, the mathematical model of QTLL system is established by using Bernoulli’s law and mass conservation. Secondly, in view of the nonlinear and coupling characteristics of the QTLL system, a input/output feedback linearization controller is designed. Then, a NDOB is proposed to estimate disturbances and applied to compensation control. Finally, simulation and experimental results show that the proposed strategy has better control performances than PID control and the disturbance observer-based sliding mode control (DOBSMC).

Objective The purpose of this work is to look into the molecular mechanisms underlying the main ferroptosis-related genes in psoriasis (PsD) and atherosclerosis (AS). Methods Download the dataset between PsD patients and normal group (GSE30999) from the GEO database, while obtaining the dataset between AS patients and normal group (GSE100927). Using R software and the limma package in Bioconductor, differentially expressed genes (DE-FRG) were obtained. On the intersecting DE-FRG, enrichment analyses using GO and KEGG were conducted. The functional interactions between DE-FRG were then examined using a protein-protein interaction (PPI) network that was built using the STRING database. In order to analyze the relationship between infiltrating immune cells and Hub genes, the immune cell infiltration in PsD and AS tissues was assessed using CIBERSORT. The DGIdb database was utilized to choose potential Hub gene medication candidates. Finally, a network of lncRNA, miRNA, and mRNA associated to Hub gene was developed. Results 133 DE-FRGs in total, including 67 up-regulated genes and 66 down-regulated genes, were examined. As a total of the screening, 215 DEGs—including 171 up-regulated genes and 44 down-regulated genes—were obtained. DE-FRG was found to be strongly enriched for the FOXO signaling pathway, ferroptosis, fluid shear stress and atherosclerosis, mTOR signaling pathway, relaxin signaling pathway, and AMPK signaling pathway, according to functional enrichment analysis. It was considerably enriched for chemokines, signaling receptor activators, granulocyte chemotactic response, lipopolysaccharide metabolism, and mesovirus defense. Eight genes, including PTEN, STAT3, MAPK3, SIRT1, IL6, HRAS, EGFR, and PPARG, were subsequently included to the Cytoscape Hubba plug-in and the ROC diagnostic curve as Hub genes. Additionally, 222 medicines that target 8 marker genes in total were obtained. On the other hand, the ceRNA network, which was based on Hub genes, revealed intricate regulatory relationships. Additionally, CIBERSORT analysis demonstrated that PTEN, STAT3, MAPK3, SIRT1, IL6, HRAS, EGFR, and PPARG alterations in the immunological milieu of AS and PsD patients may be connected. Conclusion The results could lead to new understandings of the pathogenesis of PsD and AS as well as the discovery of new ferroptosis genes that could be used as potential therapeutic targets in clinical settings or as widely representative reference markers.

Chenchen Niu,1 Xiangxiang Meng,2 Fei Xiang1 1School of Management, Qilu University of Technology, Jinan, People’s Republic of China; 2Department of Business Management, Shandong University of Finance and Economics, Jinan, 250353, People’s Republic of ChinaCorrespondence: Chenchen Niu, Tel +86-153-7666-3653, Email ncc0811@163.comPurpose: Drawing on social exchange theory and attribution theory, this study aims to explore the influencing mechanism of paradoxical leadership on organizational citizenship behavior.Participants and Methods: According to the research purpose, this study selects enterprises in the manufacturing, financial and high-tech industries in Shandong Province as the research objects, and collects data on the leaders and employees of the human resources departments and marketing departments in the enterprises. Data were collected from 77 leaders and 473 employees in China by a two-wave questionnaire survey. Hierarchical regression analysis and structural equation model approach were employed to test hypotheses.Results: This study found that perceived insider status and psychological entitlement play mediating roles about paradoxical leadership and organizational citizenship behavior; Collectivism moderates the relationship between paradoxical leadership and perceived insider status, and moderates the positive mediating role played by perceived insider status in the relationship of paradoxical leadership and organizational citizenship behavior; Leader-member exchange differentiation moderates the relationship about paradoxical leadership and psychological entitlement, and moderates negative mediating role played by psychological entitlement in the relationship of paradoxical leadership’s and organizational citizenship behavior.Conclusion: The findings of this study offer guidance for managers to better undermine the negative effects of paradoxical leadership, and improve organizational citizenship behavior.Innovations: First, this study extends the literature on paradoxical leadership by verifying the double-edged sword effect of paradoxical leadership to organizational citizenship behavior. Second, this study enriches one’s understanding of the “black box” underlying the link between paradoxical leadership and its consequences by demonstrating the mediating roles of perceived insider status and psychological entitlement. Third, by verifying the moderating roles of collectivism and leader-member exchange differentiation, this study provides insights into the boundary conditions of the impact of paradoxical leadership.Keywords: paradoxical leadership, organizational citizenship behavior, perceived insider status, psychological entitlement, collectivism, leader-member exchange differentiation

The mechanism and stereoselectivity of an asymmetric cyclopropanation reaction between 3-alkenyl-oxindole and sulfoxonium ylide catalyzed by a chiral N,N'-dioxide-Mg(II) complex were explored using the B3LYP-D3(BJ) functional and the def2-TZVP basis set. The noncatalytic reaction occurred via a stepwise mechanism, with activation barriers of 21.6-23.5 kcal mol-1. The C2-Cα bond formed followed by the carbanion SN2 substitution, constructing a three-membered ring in spiro-cyclopropyl oxindoles, accompanied by the release of dimethylsulfoxide. The electron-withdrawing N-protecting t-butyloxy carbonyl (Boc) and acetyl (Ac) groups in isatin enhanced the local electrophilicity of the C2 atom and the repulsion between the two COPh groups in the reactants, contributing to high reactivity as well as good diastereoselectivity results. The N-Boc-3-phenacylideneoxindole coordinated to the chiral ligand (L-PiPr2) in a bidentate fashion, forming a hexacoordinate-Mg(II) complex as the reactive species. The origin of enantioselectivity was from the shielding effect of 2,6-diisopropylphenyl groups in the ligand toward the si-face of oxindole. The repulsion between the SO(CH3)2 and COPh groups in 3-alkenyl-oxindole and the neighboring ortho-iPr group in the ligand directed the re-face of ylide to attack the re-face of oxindole preferably, contributing to the high diastereoselectivity of the product. A metal-ion-ligand matching relationship was important for a good asymmetric induction effect of the chiral N,N'-dioxide-metal catalyst. A large chiral cavity in the Zn(II) catalyst weakened the shielding effect of 2,6-diisopropylphenyl groups in the ligand toward the prochiral face of oxindole, leading to inferior enantioselectivity observed in the experiment.

Postmeiotic germ cells require the lactate produced by the adjacent Sertoli cells (SCs) as their sole energy fuels. Lactate production in SCs is elaborately regulated by monitoring the transcription of the lactate dehydrogenase A (Ldha) gene. However, the transcription factors that are responsible for the control of Ldha transcription in SCs remain ill defined. Herein, the expression of forkhead box Q1 (FOXQ1), a central modulator of glucose metabolism in liver, was demonstrated in mouse testis throughout postnatal development, with maximum levels in adult specimens. At this age, FOXQ1 was immunolocalized in the nuclei of the functionally mature SCs. Testicular levels of FOXQ1 were overtly modulated by germ cells (GCs)-derived IL-1α, in a dose- and time-dependent manner. To further clarify the biological functions of FOXQ1, we disrupted the mouse Foxq1 gene using a Cas9/RNA-mediated gene targeting strategy. Foxq1-/- males were subfertile and showed oligoasthenozoospermia due to lactate deficiency. Moreover, we provided the molecular evidence that FOXQ1 may regulate lactate production by directly targeting the transactivation of the Ldha gene in SCs. From a functional standpoint, overexpression of the exogenous Ldha ameliorated Foxq1 deficiency-impaired lactate synthesis in the SCsFoxq1-/- cells. Thus, these findings collectively underscore a reproductive facet of this recently characterized transcription factor, which may operate as a novel transcriptional integrator linking energy homeostasis and nursery function in SCs.

Microbial enhanced oil recovery (MEOR) is a potential tertiary oil recovery method. However, past research has failed to describe microbial growth and metabolism reasonably, especially quantification of reaction equations and operating parameters is still not clear. The present study investigated the ability of bacteria extracted from Ansai Oilfield for MEOR. Through core flooding experiments, bacteria-treated experiments produced approximately 6.28-9.81% higher oil recovery than control experiments. Then, the microbial reaction kinetic model was established based on laboratory experimental data and mass conservation. Furthermore, the proposed model was validated by matching core flooding experiment results. Lastly, the effects of different injection parameters on bacteria growth, bacteria migration, metabolite migration, residual oil distribution, and oil recovery were studied by establishing a field-scale model. The results indicate that the injected bacteria concentration and nutrient concentration have a great influence on bacteria growth in a reservoir and the low nutrient concentration seriously restricts bacteria growth. Compared with the injected bacteria concentration, nutrient concentration has a decisive effect on bacteria and metabolite migration. The injected bacteria concentration has little effect on oil recovery, while nutrient concentration and slug volume have a significant effect on oil recovery.

Plants employ sophisticated mechanisms to control developmental processes and to cope with environmental changes at transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs), two classes of endogenous noncoding RNAs, are key regulators of gene expression in plants. Recent studies have identified the interplay between miRNAs and lncRNAs as a novel regulatory layer of gene expression in plants. On one hand, miRNAs target lncRNAs for the production of phased small interfering RNAs (phasiRNAs). On the other hand, lncRNAs serve as origin of miRNAs or regulate the accumulation or activity of miRNAs at transcription and post-transcriptional levels. Theses lncRNA-miRNA interplays are crucial for plant development, physiology and responses to biotic and abiotic stresses. In this review, we summarize recent advances in the biological roles, interaction mechanisms and computational predication methods of the interplay between miRNAs and lncRNAs in plants.

We conducted a survey to examine the influence of authentic leadership on employees' unethical pro-organizational behavior, basing our research on the theory of organizational identification. Participants were 229 employees of four firms in China. The results show that authentic leadership and employees' unethical pro-organizational behavior had an inverted U-shaped relationship, and that the relationship was mediated by organizational identification. Additionally, moral identity moderated the relationship between authentic leadership and employees' unethical pro-organizational behavior. The research results provide suggestions for reducing employees' pro-organizational unethical behavior by changing the leadership style supervisors use, thereby promoting the sustainable development of the company.

The reaction mechanism and enantioselectivity of asymmetric Michael addition reaction between alkynone (R1) with α-angelica lactone (R2) catalyzed by chiral N, N'-dioxide-Sc(III) complex were investigated at the M06/6-31G(d,p) (acetonitrile, SMD) level. The α-angelica lactone substrate could isomerize to the active enolized form in the presence of Sc(OTf)3 reagent, assisted by the counter trifluoromethanesulfonate anion OTf-. The alkynone substrate and enolized angelica lactone (or its anion) coordinated to Sc(III) center of N,N'-dioxide-Sc(III) complex catalyst simultaneously, forming a high active hexacoordinate-Sc(III) complex. The catalytic reaction occurred via a two-step mechanism, in which C2 Cγ bond formation was predicted to be the chirality-controlling step as well as the rate-determining step (RDS), affording predominant S-enantiomer. The counterion OTf- facilitated C H construction as a proton-shuttle, producing mainly E-configuration product observed in experiment. The steric repulsion from the ortho-substituent of amide moiety as well as the chiral backbone of N, N'-dioxide-Sc(III) catalyst played the key role for chiral induction in the asymmetric reaction. The less destabilizing Pauli repulsion and more stabilizing attractive interaction, especially the orbital interaction, along the si-face attack pathway enhanced the enantiodifference of the two competing pathways for high enantioselectivity. The energy barriers for E/Z isomerization of S or R-enantiomer assisted by HOTf was as high as 34.6–35.0 kcal mol−1, indicating that the product with Z-conformation was difficult to be obtained. These results were in good agreement with experimental observations.

Mitochondria have critical functions in the acclimation to abiotic and biotic stresses. Adverse environmental conditions lead to increased demands in energy supply and metabolic intermediates, which are provided by mitochondrial ATP production and the tricarboxylic acid (TCA) cycle. Mitochondria also play a role as stress sensors to adjust nuclear gene expression via retrograde signalling with the transcription factor (TF) ANAC017 and the kinase CDKE1 key components to integrate various signals into this pathway. To determine the importance of mitochondria as sensors of stress and their contribution in the tolerance to adverse growth conditions, a comparative phenotypical, physiological and transcriptomic characterisation of Arabidopsis mitochondrial signalling mutants (cdke1/rao1 and anac017/rao2) and a set of contrasting accessions was performed after applying the complex compound stress of submergence. Our results showed that impaired mitochondrial retrograde signalling leads to increased sensitivity to the stress treatments. The multi-factorial approach identified a network of 702 co-expressed genes, including several WRKY TFs, overlapping in the transcriptional responses in the mitochondrial signalling mutants and stress-sensitive accessions. Functional characterisation of two WRKY TFs (WRKY40 and WRKY45), using both knockout and overexpressing lines, confirmed their role in conferring tolerance to submergence. Together, the results revealed that acclimation to submergence is dependent on mitochondrial retrograde signalling, and underlying transcriptional re-programming is used as an adaptation mechanism.

Mitochondrial ACONITASE3 is important for the acclimation to submergence stress by integrating carbon and nitrogen metabolism and impacting stress signaling pathways. Flooding causes severe crop losses in many parts of the world. Genetic variation in flooding tolerance exists in many species; however, there are few examples for the identification of tolerance genes and their underlying function. We conducted a genome-wide association study (GWAS) in 387 Arabidopsis (Arabidopsis thaliana) accessions. Plants were subjected to prolonged submergence followed by desubmergence, and seven traits (score, water content, Fv/Fm, and concentrations of nitrate, chlorophyll, protein, and starch) were quantified to characterize their acclimation responses. These traits showed substantial variation across the range of accessions. A total of 35 highly significant single-nucleotide polymorphisms (SNPs) were identified across the 20 GWA datasets, pointing to 22 candidate genes, with functions in TCA cycle, DNA modification, and cell division. Detailed functional characterization of one candidate gene, ACONITASE3 (ACO3), was performed. Chromatin immunoprecipitation followed by sequencing showed that a single nucleotide polymorphism in the ACO3 promoter co-located with the binding site of the master regulator of retrograde signaling ANAC017, while subcellular localization of an ACO3-YFP fusion protein confirmed a mitochondrial localization during submergence. Analysis of mutant and overexpression lines determined changes in trait parameters that correlated with altered submergence tolerance and were consistent with the GWAS results. Subsequent RNA-seq experiments suggested that impairing ACO3 function increases the sensitivity to submergence by altering ethylene signaling, whereas ACO3 overexpression leads to tolerance by metabolic priming. These results indicate that ACO3 impacts submergence tolerance through integration of carbon and nitrogen metabolism via the mitochondrial TCA cycle and impacts stress signaling during acclimation to stress.

A sliding mode control (SMC) strategy using novel piecewise double power reaching law (PDPRL) for a class of fourth-order nonlinear underactuated systems (USs) is proposed to realize simultaneous control of the unactuated and the actuated part. A novel PDPRL is designed in the form of a piecewise function to reduce the chattering produced by SMC. The fixed-time convergence characteristic and the existence of maximum convergence time independent of the initial value of the sliding mode surface for the PDPRL are analyzed. Considering the uncertainties of model parameters and external disturbance in the USs, a supertwisting disturbance observer (STDO) is provided to accurately estimate the disturbance in real time, and the estimated value is compensated to the controller. The underactuated inverted pendulum system is taken to verify the effectiveness of the devised strategy, and the simulation results show that the proposed strategy can reduce chattering, suppress the disturbance, and enhance the robustness of the system.

Xiangxiang Meng,1 Niu Chenchen,2 Fu Liang,3 Yao Ocean Liu4 1Bank of Jining, Jining, 272000, People’s Republic of China; 2School of Management, Qilu University of Technology, Jinan, 250014, People’s Republic of China; 3Department of Business Management, Shandong University of Finance and Economics, Jinan, 250353, People’s Republic of China; 4School of Professional Studies, New York University, New York City, NY, USACorrespondence: Niu Chenchen Tel +86-153-7666-3653Email ncc0811@163.comPurpose: According to attribution theory, the purpose of this paper is to examine the relationship between paradoxical leadership and employees’ compulsory organizational citizenship behavior, research the mediating and moderating effects of work pressure perception and leadership member exchange differentiation.Participants and Methods: The data collection work was carried out with 329 employees as the research object, and empirical tests were carried out using confirmatory factor analysis and hierarchical regression analysis methods.Results: The results of the empirical test show that: paradoxical leadership has a significant positive effect on employees’ compulsory organizational citizenship behavior; work pressure perception plays a mediating role between paradoxical leadership and employee’s compulsory organizational citizenship behavior; leadership member exchange differentiation plays a moderating role between paradoxical leadership and work pressure perception. Paradoxical leadership will lead employees to adopt compulsory organizational citizenship behavior. Moreover, organizations should reduce employees’ perception of work pressure and leadership member exchange differentiation.Conclusion: The results of the study provide positive suggestions for reducing the compulsory organizational citizenship behavior of employees by focusing on the behavior of paradoxical leadership, thereby promoting the improvement of corporate performance.Innovations: 1) The research on the influence of paradoxical leadership on compulsory organizational citizenship behavior reveals the “dark side” of the influence of paradoxical leadership on employee behavior. 2) By introducing work pressure perception, this paper reveals the mechanism of action between paradoxical leadership and compulsory organizational citizenship behavior, which provides a new research perspective for understanding the formation mechanism of employees adopting compulsory organizational citizenship behavior. 3) This paper confirms that leadership member exchange differentiation can positively moderate the relationship between paradoxical leadership and work pressure perception.Keywords: attribution theory, paradoxical leadership, compulsory organizational citizenship behavior, work pressure perception, leadership member exchange differentiation

Phosphate (Pi) and iron (Fe) are two essential mineral nutrients for plant growth and development. Pi starvation triggers the Fe local redistribution and over-accumulation, resulting in the reduction of the primary root, while represses the expression of Fe uptake genes. Nevertheless, the antagonistic mechanism between P and Fe nutrition in plant remain not addressed. Here, the effect of the upregulated expression of Fe regulators IMA1 and bHLH104 driven by the different-type promoters (proCaMV 35S, the promoters of Pi-starvation responsive genes proIPS1 and proPHT1;4) in response to Pi starvation was investigated in Arabidopsis. The results showed that the expression of Fe uptake genes IRT1 and FRO2 was successfully upregulated in proIPS1::IMA1, proPHT1;4::IMA1 and proIPS1::bHLH104 under Pi starvation while decreased in pro35S::IMA1, pro35S::bHLH104 and proPHT1;4::bHLH104, compared with that in the corresponding plants under Pi sufficiency. Although the length and Fe distribution in roots of them didn’t have significant difference with wild type under Pi starvation, the Fe distribution and total Fe contents were significantly increased in shoots of proIPS1::IMA1, proPHT1;4::IMA1 and proIPS1::bHLH104 while were decreased in proPHT1;4::bHLH104. The higher Fe concentrations in the Pi-starved transgenic plants also conferred the obviously tolerance to Fe deficiency. Their biomasses and total P concentrations showed no difference with wild type, regardless of Pi sufficiency or deficiency. Therefore, this approach would be a novel manipulation to modify Fe nutrient via coupling with Pi starvation in plants.

A novel method of disturbance observer-based integral backstepping control is proposed for the two-tank liquid level system with external disturbances. The problem of external disturbances can be settled in this paper. Firstly, the mathematical model of the two-tank liquid level system is established based on fluid mechanics and principle of mass conservation. Secondly, an integral backstepping control strategy is designed in order to ensure liquid level tracking performance by making the tracking errors converge to zero in finite time. Thirdly, a disturbance observer is designed for the two-tank liquid level system with external disturbances. Finally, the validity of the proposed method is verified by simulation and experiment. By doing so, the simulation and experimental results prove that the scheme of disturbance observer-based integral backstepping control strategy can suppress external disturbances more effective than the disturbance observer-based sliding mode control method and has better dynamic and steady performance of the two-tank liquid level system.

Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr.), a deciduous conifer, widely grows in middle and high elevations of Northern China. Its natural distribution has sharply decreased and has become fragmented, which may have resulted in the loss of genetic variation. In this study, ten natural populations across the entire range of this species were analyzed using amplified fragment length polymorphism markers. A total of 309 loci were detected from 225 individuals of these populations, of which 261 (84.5%) were polymorphic. At the species level, the genetic diversity was high (average of the Nei’s genetic diversity He = 0.2602, and Shannon’s information index I = 0.3967). The results of molecular variance analysis showed that 90.71% of the genetic diversity occurred within populations. The genetic differentiation among populations was moderate as a whole (FST = 0.0929, GST = 0.1510), which is consistent with the moderate level of gene flow among populations (Nm = 2.8116). Based on the unweighted pair group method with arithmetic mean and STRUCTURE analysis, these populations were grouped into three genetically distinct clusters. The degree of inter-population differentiation (GST = 0.1338) for the south group was larger than that for the north group (GST = 0.0915). There was a significant correlation between genetic distance and geographic distance across the species range (r = 0.316, P

We reported a mechanistic study on asymmetric O–H insertion reaction of α-diazoester with carboxylic acid using Rh2(OAc)4/chiral guanidine-amide as the cocatalyst by density functional theory [B3LY...

Terpene trilactones (TTLs) are the main secondary metabolites of Ginkgo biloba. As one of the rate-limiting enzymes in the mevalonic acid (MVA) pathway of TTL biosynthesis, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the 3-hydroxy-3-methylglutaryl coenzyme A to form MVA. In this study, two cDNA sequences of HMGR genes, namely, GbHMGR2 and GbHMGR3, were cloned from G. biloba. The protein sequences of GbHMGR2 and GbHMGR3, which contain several functional domains, were analyzed. Regulatory elements related to light, hormone, and stress response were detected in the promoter regions of GbHMGR2 and GbHMGR3. The catalytic activity of these genes was verified by a functional complement experiment in yeast. Quantitative real-time PCR (qRT-PCR) showed the distinct expression patterns of the two genes in different organs. The TTL contents in the organs were detected by high-performance liquid chromatography– evaporative light scattering detector. GbHMGR2 and GbHMGR3 were responded to cold, dark, methyl jasmonate (MJ), abscisic acid (ABA), salicylic acid (SA), and ethephon (Eth) treatments. The TTL contents were also regulated by cold, dark, MJ, ABA, SA, and Eth treatment. In conclusion, GbHMGR2 and GbHMGR3 may participate in the MVA pathway of TTL biosynthesis.