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An immunochromatographic test strip method for detecting A29 was established. The strips utilizing mAb-7C5 and 5D8 showed the best sensitivity with the lowest LOD, 50 pg mL−1 for A29.

The synthetic antibiotics fluoroquinolones are popular due to their good antibacterial performance and low price, but the risk to human health caused by their residues has attracted great attention. In this study, an ultra-sensitive mAb, 4D7, was prepared with an IC

Spinosad (SPI) and spinetoram (Et-SPI) are currently among the most popular new insecticides because of their high efficiency and low toxicity. However, excessive residues in food still pose a potential risk to public health. Therefore, it is necessary to strengthen residue monitoring of the two insecticides based on a simple and rapid method. In this study, a highly sensitive mAb (6G9) against SPI and Et-SPI was prepared using the hapten SPI-HS and used to develop a colloidal gold nanoparticle-based immunochromatographic strip for the detection of SPI and Et-SPI in samples. The quantitative ranges of the developed strip for SPI and Et-SPI were 8.93-1633 ng g

Fentanyl is a typical opioid that is used in surgical anesthesia. However, when abused, fentanyl can lead to addiction and even death. To better control the use of fentanyl, it is necessary to develop rapid and sensitive detection methods. In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum. Under optimum conditions, the anti-fentanyl mAb belonging to the subtype of IgG2b showed a half-maximal inhibitory concentration (IC50) of 0.11 ng mL-1 and a linear range of detection of 0.020-0.50 ng mL-1. Fenanyl-spiked original urine and serum diluted eight times were used for the analysis of fentanyl by ic-ELISA and CG-ICS. IC50 from the standard curves was 0.46 ng mL-1 for urine and 2.6 ng mL-1 for serum in ic-ELISA and 1.6 ng mL-1 for urine and 6.27 ng mL-1 for serum in CG-ICS. The recovery test revealed that the ic-ELISA and CG-ICS, with a recovery rate of 87.0-108.4% and a coefficient of variation of 3.3-10.9%, were the same reliable tools as the liquid chromatography tandem mass spectrometry for fentanyl analysis in real samples.

Tricaine (TMS) is usually used as an anesthetic in the transportation of aquatic animals. However, when consumed, the numerous residual drug residues found in fish can cause a high risk to human health. Therefore, it is important to develop a rapid and sensitive method for the detection of this drug in food. Here we have developed a novel lateral-flow immunochromatographic strip (LF-ICS) method for the detection of TMS in fish samples, using a very effective anti-TMS monoclonal antibody with a half-maximal inhibitory concentration value of 31.79 ng/mL. After the K2CO3 and coating antigen concentrations were optimized in phosphate buffered saline (pH 7.4), the LF-ICS was used to determine TMS levels in fish samples spiked with varying concentrations of the drug. Following this, the values for the visual limit of detection and the cut-off for TMS were found to be less than 0.1 and 1 μg/mL, respectively. These results indicated that our developed LF-ICS is applicable for on-site screening of TMS in fish samples.

Enrofloxacin, a veterinary antibiotic that persists in food, poses a risk to human health. Here, a monoclonal antibody against enrofloxacin, 1H12, was prepared based on the hapten ENR-1, and showed excellent sensitivity with a 50% inhibitory concentration (IC

Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was synthesized and conjugated to keyhole limpet hemocyanin by mixed anhydride method as immunogen, whereas TML was connected to ovalbumin with 1,1′-carbonyldiimidazole as coating antigen. Under optimized conditions, the ultrasensitive monoclonal antibody-based ic-ELISA exhibited a half-maximal inhibitory concentration (IC50) value of 0.36 ng/mL with a working range of 0.14–0.9 ng/mL for TML. A rapid and sensitive immunochromatographic strip assay was developed with a TML cutoff value of 2.5 ng/mL. On the basis of these results, both developed methods are useful for TML detection in chicken.

An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples. The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2 ng/mL for FF and 0.27 ng/mL for TAP, with the working range of 0.05–0.77 and 0.05–1.42 ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1 ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.