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This study used a porcine model to systematically investigate whether carboxyfullerene C60(CF-C60) can be used for sperm preservation. The results indicated that CF-C60supplementation can preserve porcine sperm quality during storage at 17 °C. This effect was attributable to an improvement in the antioxidant capacity of sperm through a decrease in the reactive oxygen species (ROS) level. Additionally, CF-C60can maintain mitochondrial function, inhibit sperm apoptosis through the ROS/Cytochrome C (Cyt C)/Caspase 3 signaling pathway, and mediate suppression of bacterial growth through the effects of ROS. Finally, the results of artificial insemination experiments indicated that insemination with CF-C60-treated sperm can increase the total number of offspring born and reduce the number of deformed piglets. Thus, CF-C60is safe for use as a component of semen diluent for sperm storage.This research unveils that CF-C60improves porcine sperm quality by decreasing the levels of ROS to enhance antioxidation, antiapoptosis, and antibiosis during liquid storage at 17 °C. The findings provide a novel technical basis for expanding the application of carbon nano-materials as antioxidants in porcine reproduction.The development of novel porcine sperm protective agents holds profound significance for improving fertility quality and promoting reproductive health. Excessive oxidative stress and bacterial contamination, leading to sperm apoptosis, are the 2 major factors affecting the decline of porcine sperm quality. Recently, CF-C60has gained attention as an important nanocarbon derivative with strong antioxidant and antibacterial activity. However, the role and mechanism of CF-C60in the preservation of mammalian sperm remain unknown. This study aimed to explore the important protective role of CF-C60in porcine sperm.