120 results on '"Xiao Jun He"'
Search Results
2. Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes
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Qin Ping Yu, Ding Yuan Feng, Xiao Jun He, Fan Wu, Min Hao Xia, Tao Dong, Yi Hua Liu, Hui Ze Tan, Shi Geng Zou, Tao Zheng, Xian Hua Ou, and Jian Jun Zuo
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Muscle Fiber ,Cell Proliferation ,Traditional Chinese Medicine Formula ,Pigs ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
Objective This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula’s extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p
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- 2017
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3. Emerging blood exosome-based biomarkers for preclinical and clinical Alzheimer’s disease: a meta-analysis and systematic review
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Wei-Lin Liu, Hua-Wei Lin, Miao-Ran Lin, Yan Yu, Huan-Huan Liu, Ya-Ling Dai, Le-Wen Chen, Wei-Wei Jia, Xiao-Jun He, Xiao-Ling Li, Jing-Fang Zhu, Xie-Hua Xue, Jing Tao, and Li-Dian Chen
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alzheimer’s disease ,amyloid-β ,biomarkers ,blood ,exosomes ,extracellular vesicles ,meta-analysis ,mild cognitive impairment ,systematic review ,tau protein ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Blood exosomes, which are extracellular vesicles secreted by living cells into the circulating blood, are regarded as a relatively noninvasive novel tool for monitoring brain physiology and disease states. An increasing number of blood cargo-loaded exosomes are emerging as potential biomarkers for preclinical and clinical Alzheimer’s disease. Therefore, we conducted a meta-analysis and systematic review of molecular biomarkers derived from blood exosomes to comprehensively analyze their diagnostic performance in preclinical Alzheimer’s disease, mild cognitive impairment, and Alzheimer’s disease. We performed a literature search in PubMed, Web of Science, Embase, and Cochrane Library from their inception to August 15, 2020. The research subjects mainly included Alzheimer’s disease, mild cognitive impairment, and preclinical Alzheimer’s disease. We identified 34 observational studies, of which 15 were included in the quantitative analysis (Newcastle-Ottawa Scale score 5.87 points) and 19 were used in the qualitative analysis. The meta-analysis results showed that core biomarkers including Aβ1–42, P-T181-tau, P-S396-tau, and T-tau were increased in blood neuron-derived exosomes of preclinical Alzheimer’s disease, mild cognitive impairment, and Alzheimer’s disease patients. Molecules related to additional risk factors that are involved in neuroinflammation (C1q), metabolism disorder (P-S312-IRS-1), neurotrophic deficiency (HGF), vascular injury (VEGF-D), and autophagy-lysosomal system dysfunction (cathepsin D) were also increased. At the gene level, the differential expression of transcription-related factors (REST) and microRNAs (miR-132) also affects RNA splicing, transport, and translation. These pathological changes contribute to neural loss and synaptic dysfunction. The data confirm that the above-mentioned core molecules and additional risk-related factors in blood exosomes can serve as candidate biomarkers for preclinical and clinical Alzheimer’s disease. These findings support further development of exosome biomarkers for a clinical blood test for Alzheimer’s disease. This meta-analysis was registered at the International Prospective Register of Systematic Reviews (Registration No. CRD4200173498, 28/04/2020).
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- 2022
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4. Clinical Characteristics and Prognostic Factors of Early and Late Recurrence After Definitive Radiotherapy for Nasopharyngeal Carcinoma
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Feng Li, Fo-Ping Chen, Yu-Pei Chen, Yue Chen, Xiao-Jun He, Xiao-Dan Huang, Zi-Qi Zheng, Wei-Hong Zheng, Xu Liu, Ying Sun, and Guan-Qun Zhou
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nasopharyngeal carcinoma ,radiotherapy ,prognosis ,early locoregional recurrence ,late locoregional recurrence ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
We investigated the clinical characteristics, prognostic factors, and post-recurrence prognostic factors of early- and late-recurrence patients for nasopharyngeal carcinoma (NPC) after definitive intensity-modulated radiation therapy (IMRT). This was a single-center retrospective analysis of patients in China from January 2010 to December 2015. The prognostic factors for overall survival (OS) and post-recurrence OS of early- and late-recurrence patients were identified using univariate and multivariate Cox regression analyses. Of the 9,468 patients included, 409 (4.3%), 325 (3.4%), and 182(1.9%) developed purely local recurrence, purely regional recurrence, and locoregional recurrence during follow-up, respectively. In the purely local recurrence group, 192 patients (46.9%) developed early local recurrence (ETR), and 217 patients (53.1%) developed late local recurrence (LTR). Of the 192 ETR patients, multivariate Cox regression analysis revealed that age and gender were independent risk factors of OS, and post-recurrence best supportive treatment (PRBST) was associated with poorer post-recurrence OS. Of the 217 LTR patients, the results revealed that baseline value of EBV-DNA was an independent risk factor for OS, while PRBST was associated with poorer post-recurrence OS. In the purely regional recurrence group, 183 patients (56.3%) developed early regional recurrence (ENR), and 142 patients (43.7%) developed late regional recurrence (LNR). Of the 183 ENR patients, multivariate Cox regression analysis revealed that alcohol abuse and TNM stage were independent risk factors of OS, while alcohol drinkers and PRBST were associated with poorer post-recurrence OS. Of the 142 LNR patients, PRBST was associated with poorer post-recurrence OS. In the locoregional recurrence group, 87 patients (47.8%) developed early locoregional recurrence (ELR), and 95 patients (52.2%) developed late locoregional recurrence (LLR). Of the 87 ELR patients, multivariate Cox regression analysis revealed that N stage and TNM stage were independent risk factors of OS, and N2/3 stage and PRBST were associated with poorer post-recurrence OS. Of the 95 LLR patients, the results revealed that T stage was an independent risk factor for OS, while T3/4 stage and PRBST were associated with poorer post-recurrence OS. Patients with LTR/LNR/LLR demonstrate significantly better OS compared with patients with ETR/ENR/ELR, Nevertheless, post-recurrence OS between patients with ETR/ENR/ELR and LTR/LNR/LLR was not significantly different.
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- 2020
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5. Prognostic Value of Circulating Lipoprotein in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma
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Ji-Jin Yao, Xiao-Jun He, Wayne R. Lawrence, Wang-Jian Zhang, Jia Kou, Fan Zhang, Guan-Qun Zhou, Si-Yang Wang, and Ying Sun
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Nasopharyngeal carcinoma ,Locoregionally advanced ,Lipoprotein ,Ratio ,Prognostic value ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Lipoproteins have been reported to be associated with prognosis in various cancers; however, the prognostic value of lipoproteins in patients with nasopharyngeal carcinoma (NPC) remains largely unknown. We aim to asses the role of circulating lipoproteins in locoregionally advanced NPC patients. Methods: Between October 2009 and August 2012, a total of 1,081 patients with stage III-IVB NPC were included in the analysis. Circulating high-density lipoprotein (HDL) and low-density lipoprotein (LDL) are the two key lipoproteins, which were measured at baseline. Receiver operating characteristic (ROC) curve analysis was used to evaluate different cut-off points for lipoproteins. Actuarial rates were performed using Kaplan–Meier methods and the log-rank test. Results: The cutoff points of HDL, LDL, and LDL/HDL ratio were 1.17 mmol/L, 3.75 mmol/L, and 2.73, respectively. At 5 years, high HDL (> 1.17 mmol/L) was significantly associated with better overall survival (OS, 86.6% vs. 78.9%; P=0.004), distant metastasis-free survival (DMFS, 86.9% vs. 80.8%; P=0.004), locoregional relapse-free survival (LRFS, 90.8% vs. 85.4%; P=0.010), and progression-free survival (PFS, 79.1% vs. 70.2%; P= 0.001) than low HDL (≤1.17 mmol/L). In contrast, high LDL (> 3.75 mmol/L) tend to be inferior OS (79.1% vs. 84.9%; P= 0.016) in compassion with low LDL (≤3.75 mmol/L). Likewise, patients with high LDL/HDL ratio (> 2.73) tend to be inferior OS (79.3% vs. 86.9%; P=0.001), DMFS (81.9% vs. 86.5%; P=0.030), and PFS (72.6% vs. 77.8%; P= 0.034) than those of low LDL/HDL ratio (≤2.73). In multivariate analysis, baseline HDL was found to be a significant prognostic factor for LRFS (HR= 0.65; 95% CI, 0.45-0.93; P= 0.019) and PFS (HR=0.75; 95% CI, 0.58-0.98; P= 0.034). Conclusions: Circulating HDL is significantly associated with treatment outcomes in patients with locoregionally advanced NPC. We suggest that HDL measurements will be of great clinical significance in the management of NPC.
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- 2018
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6. N/S co-doped interconnected porous carbon nanosheets as high-performance supercapacitor electrode materials
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Yu-chen WEI, Jian ZHOU, Lei YANG, Jing GU, Zhi-peng CHEN, and Xiao-jun HE
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Materials Science (miscellaneous) ,General Materials Science ,General Chemistry - Published
- 2022
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7. Supplementary Table S9 from Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3
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Ying Sun, Na Liu, Jun Ma, Ying-Qin Li, Xin Wen, Jian Zhang, Jia Kou, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Xiao-Dan Huang, Jia-Wei Lv, Lu-Lu Zhang, Li Lin, Guan-Qun Zhou, Zhi-Xuan Li, and Zi-Qi Zheng
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Supplementary Table S9. The predicted miRNAs that bind with FAM225A according to DIANA Tools
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- 2023
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8. Supplemental Tables from Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance
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Ying Sun, Na Liu, Jun Ma, Si-Si Xu, Feng Li, Ying-Qin Li, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Guan-Qun Zhou, Lu-Lu Zhang, Jia-Wei Lv, Jia Kou, Li Lin, Yue Chen, Jun-Yan Li, Xu Liu, Jia-Li Guan, Zhi-Xuan Li, and Zi-Qi Zheng
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Supplemental Table S1. qRT-PCR primers used in this study Supplemental Table S2. siRNA sequence used in this study Supplemental Table S3. Primers used for shRNA plasmid construction Supplemental Table S4. Primers used for truncation variants plasmid construction Supplemental Table S5. Primers sequence used for ChIP-PCR assay Supplemental Table S6. Probe sequence of TINCR in situ hybridization (ISH) Supplemental Table S7. Clinical characteristics of nasopharyngeal carcinoma patients according to the high and low expression of TINCR Supplemental Table S8. Univariate and multivariable Cox regression analysis of TINCR expression level and survival Supplemental Table S9. The top10 proteins found by mass spectrometry analysis in the antisense-TINCR group (negative control). Supplemental Table S10. The top10 proteins found by mass spectrometry analysis in the TINCR group. The bold portion shows the proteins that overlap antisense-TINCR
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- 2023
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9. Data from Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance
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Ying Sun, Na Liu, Jun Ma, Si-Si Xu, Feng Li, Ying-Qin Li, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Guan-Qun Zhou, Lu-Lu Zhang, Jia-Wei Lv, Jia Kou, Li Lin, Yue Chen, Jun-Yan Li, Xu Liu, Jia-Li Guan, Zhi-Xuan Li, and Zi-Qi Zheng
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Frontier evidence suggests that dysregulation of long noncoding RNAs (lncRNA) is ubiquitous in all human tumors, indicating that lncRNAs might have essential roles in tumorigenesis. Therefore, an in-depth study of the roles of lncRNA in nasopharyngeal carcinoma (NPC) carcinogenesis might be helpful to provide novel therapeutic targets. Here we report that lncRNA TINCR was significantly upregulated in NPC and was associated positively with poor survival. Silencing TINCR inhibited NPC progression and cisplatin resistance. Mechanistically, TINCR bound ACLY and protected it from ubiquitin degradation to maintain total cellular acetyl-CoA levels. Accumulation of cellular acetyl-CoA promoted de novo lipid biosynthesis and histone H3K27 acetylation, which ultimately regulated the peptidyl arginine deiminase 1 (PADI1)–MAPK–MMP2/9 pathway. In addition, insulin-like growth factor 2 mRNA-binding protein 3 interacted with TINCR and slowed its decay, which partially accounted for TINCR upregulation in NPC. These findings demonstrate that TINCR acts as a crucial driver of NPC progression and chemoresistance and highlights the newly identified TINCR–ACLY–PADI1–MAPK–MMP2/9 axis as a potential therapeutic target in NPC.Significance:TINCR-mediated regulation of a PADI1–MAPK–MMP2/9 signaling pathway plays a critical role in NPC progression and chemoresistance, marking TINCR as a viable therapeutic target in this disease.
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- 2023
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10. Data from Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3
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Ying Sun, Na Liu, Jun Ma, Ying-Qin Li, Xin Wen, Jian Zhang, Jia Kou, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Xiao-Dan Huang, Jia-Wei Lv, Lu-Lu Zhang, Li Lin, Guan-Qun Zhou, Zhi-Xuan Li, and Zi-Qi Zheng
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Long noncoding RNAs (lncRNA) play important roles in the tumorigenesis and progression of cancers. However, the clinical significance of lncRNAs and their regulatory mechanisms in nasopharyngeal carcinogenesis (NPC) are largely unknown. Here, based on a microarray analysis, we identified 384 dysregulated lncRNAs, of which, FAM225A was one of the most upregulated lncRNAs in NPC. FAM225A significantly associated with poor survival in NPC. N(6)-Methyladenosine (m6A) was highly enriched within FAM225A and enhanced its RNA stability. FAM225A functioned as an oncogenic lncRNA that promoted NPC cell proliferation, migration, invasion, tumor growth, and metastasis. Mechanistically, FAM225A functioned as a competing endogenous RNA (ceRNA) for sponging miR-590-3p and miR-1275, leading to the upregulation of their target integrin β3 (ITGB3), and the activation of FAK/PI3K/Akt signaling to promote NPC cell proliferation and invasion. In summary, our study reveals a potential ceRNA regulatory pathway in which FAM225A modulates ITGB3 expression by binding to miR-590-3p and miR-1275, ultimately promoting tumorigenesis and metastasis in NPC.Significance:These findings demonstrate the clinical significance of the lncRNA FAM225A in nasopharyngeal carcinoma (NPC) and the regulatory mechanism involved in NPC development and progression, providing a novel prognostic indicator and promising therapeutic target.
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- 2023
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11. Supplementary Tables S1-S5, S7-S8, S10 from Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3
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Ying Sun, Na Liu, Jun Ma, Ying-Qin Li, Xin Wen, Jian Zhang, Jia Kou, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Xiao-Dan Huang, Jia-Wei Lv, Lu-Lu Zhang, Li Lin, Guan-Qun Zhou, Zhi-Xuan Li, and Zi-Qi Zheng
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Supplementary Table S1. qPCR primers used in this study Supplementary Table S2. The FAM225A expression level and grouping of samples based on GEO dataset (GSE12452) Supplementary Table S3. Primers used for shRNA plasmid construction Supplementary Table S4. Sequence of miRNA mimic or inhibitor used in this study Supplementary Table S5. Probe sequence of FAM225A in situ hybridization (ISH) Supplementary Table S7. Clinical characteristics of nasopharyngeal carcinoma patients according to the high and low expression of FAM225A. Supplementary Table S8. Univariate and multivariable Cox regression analysis of FAM225A expression level and survival. Supplementary Table S10. Inguinal lymph node metastasis in vivo.
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- 2023
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12. Supplementary Figures from Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance
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Ying Sun, Na Liu, Jun Ma, Si-Si Xu, Feng Li, Ying-Qin Li, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Guan-Qun Zhou, Lu-Lu Zhang, Jia-Wei Lv, Jia Kou, Li Lin, Yue Chen, Jun-Yan Li, Xu Liu, Jia-Li Guan, Zhi-Xuan Li, and Zi-Qi Zheng
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Supplemental Figure S1. TINCR is upregulated in six other cancer types. Supplemental Figure S2. GSEA analyses results of TINCR silencing. Supplemental Figure S3. Overexpression of TINCR promotes NPC cell proliferation, metastasis and cisplatin resistance in vitro. Supplemental Figure S4. Overexpression of TINCR reverses the suppressive effects of TINCR knockdown on cell proliferation, cisplatin resistance and metastasis. Supplemental Figure S5. TINCR targets ACLY and impairs its ubiquitination degradation. Supplemental Figure S6. Silencing TINCR has no effects on the expressions of KAT3A and KAT3B. Supplemental Figure S7. The ACLY-binding motif CUGKR is critical for the role of TINCR in maintaining ACLY protein stability and lipid synthesis levels. Supplemental Figure S8. Acetyl-CoA is responsible for TINCR-mediated NPC progression and chemoresistance. Supplemental Figure S9. Correlation between TINCR and PADI1 expression in 16 other cancer types from the TCGA database. Supplemental Figure S10. Silencing TINCR decreases the proteolytic activities of MMP2/9, but had no effects on H3K27ac levels at MMP2/9 promoters. Supplemental Figure S11. IGFBP3 is overexpressed in NPC
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- 2023
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13. Supplementary Figures S1-S11 from Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3
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Ying Sun, Na Liu, Jun Ma, Ying-Qin Li, Xin Wen, Jian Zhang, Jia Kou, Xiao-Jun He, FoPing Chen, Rui-Qi Liu, Xiao-Dan Huang, Jia-Wei Lv, Lu-Lu Zhang, Li Lin, Guan-Qun Zhou, Zhi-Xuan Li, and Zi-Qi Zheng
- Abstract
Supplementary Figure S1. FAM225A is overexpressed in NPC and other tumor types. Supplementary Figure S2. Silencing METTL3 resulted in the decreased m6A level of total RNA. Supplementary Figure S3. FAM225A promotes NPC cell proliferation, migration and invasion in vitro. Supplementary Figure S4. FISH assay showed that FAM225A was mainly located in cytoplasmic. Supplementary Figure S5. FAM225A acts as a ceRNA for miR-590-3p and miR-1275. Supplementary Figure S6. The expression of FAM225A is negatively correlated with miR-590-3p and miR-1275 expression. Supplementary Figure S7. Silencing of miR-590-3p and miR-1275 endows NP69 and N2Tert cells with carcinogenicity in vitro. Supplementary Figure S8. miR-590-3p/miR-1275 are responsible for FAM225A-mediated proliferation, migration and invasion. Supplementary Figure S9. Correlation between FAM225A and ITGB3 expression in 12 other cancer types from the TCGA database. Supplementary Figure S10. FAM225A promotes NPC cell metastasis in vivo. Supplementary Figure S11. ITGB3 is responsible for FAM225A-meidated tumorigenesis and metastasis in vivo.
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- 2023
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14. Voronoi tessellation and hierarchical model based texture image segmentation
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Quan-hua ZHAO, Yu LI, Xiao-jun HE, and Wei-dong SONG
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texture segmentation ,Voronoi tessellation ,bivariate Gaussian Markov random field (BGMRF) ,Bayesian inference ,maximum a posterior (MAP) ,Telecommunication ,TK5101-6720 - Abstract
A regional and statistical based algorithm for texture image segmentation was proposed. The Voronoi tessella-tion was used for partitioning the domain of an image into sub-regions corresponding to the components of homogenous regions, to which the texture image needs to be segmented. Bivariate Gaussian Markov random field (BGMRF) model, static random field, and potts model were employed to characterize the interactions between two neighbor pixel pairs in a sub-region, and among sub-regions, respectively. Following Bayesian paradigm, a posterior distribution, which models the texture segmentation for a given texture image, was obtained. A metropolis-hastings algorithm was designed for simulating the posterior distribution. Then, texture segmentation was obtained by maximum a posterior (MAP) scheme. The proposed algorithm was tested with both of synthesized and real texture images. The results are qualitatively and quantitatively evaluated and show that the proposed algorithm works well on both of texture images.
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- 2014
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15. Improving biliary stent patency for malignant obstructive jaundice using endobiliary radiofrequency ablation: experience in 150 patients
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Cheng Wang, Jing Zhao, Xiao-Jun He, Gang Zhao, Chengli Liu, Yalin Kong, Hong-yi Zhang, and Ling-Hong Kong
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medicine.medical_specialty ,Percutaneous ,Radiofrequency ablation ,medicine.medical_treatment ,Percutaneous transhepatic cholangiography ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Internal medicine ,Carcinoma ,Humans ,Medicine ,Retrospective Studies ,Radiofrequency Ablation ,Cholestasis ,business.industry ,Stent ,Hepatology ,Ablation ,medicine.disease ,Surgery ,Jaundice, Obstructive ,Treatment Outcome ,surgical procedures, operative ,Bile Duct Neoplasms ,030220 oncology & carcinogenesis ,Catheter Ablation ,Quality of Life ,Stents ,030211 gastroenterology & hepatology ,business ,Abdominal surgery - Abstract
Although self-expandable mental stents (SEMS) placement is the standard care for relieving obstructive jaundice caused by unresectable malignant biliary stricture, how to maintain stent potency remains an intractable problem. This study was to evaluate the efficacy and safety of endobiliary radiofrequency ablation (RFA) through percutaneous transhepatic cholangiography (PTC) pathway in treating such patients. Consecutive patients who were performed endobiliary RFA as well as SEMS placement because of unresectable malignant obstructive jaundice in single institution in recent 8 years were retrospectively reviewed. As comparison, patients who underwent only percutaneous SEMS placement for unresectable malignant biliary stricture during the contemporary period were reviewed. Stent patency, complications, complications, and overall survival (OS) were investigated and analyzed. One hundred and fifty patients who underwent endobiliary RFA and 127 patients who underwent only stent placement were included in this study. In the study group of endobiliary RFA, 87 patients (58.0%) underwent ablation for 1 time, 49 (32.7%) for 2 times, and 14 (9.3%) for 3 times. Complications related to RFA as well as SEMS placement happened in 113 patients (75.3%), without severe complications that needed emergent surgery or interventional therapy. The median duration of stent patency after ablation was 11.2 month, and the median survival time was 12.3 month. As comparison, difference was found in the number of interventional procedures and stents placed, duration of initial stent patency, and the incidence of moderate bleeding and pain. In the study group, only the type of tumor that caused biliary obstruction (intrahepatic carcinoma vs. extrahepatic carcinoma) was a poor independent factor (P = 0.035) for recurrent biliary obstruction. Repeated interventional therapy and adoption of subsequent therapy were only independent factors for OS. Endobiliary RFA and SEMS placement is technically safe and feasible for unresectable malignant obstructive jaundice to improve the quality of life and prolong survival.
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- 2021
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16. Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy
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Zhenhai Cai, Gang Chen, Chenglong Huang, Kang Ji, Xiao-Jun He, Hongwei Xu, and Zhonghai Shen
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autophagy ,Aging ,Indoles ,Tubastatin A ,Apoptosis ,Histone Deacetylase 6 ,Hydroxamic Acids ,medicine.disease_cause ,Chondrocyte ,Extracellular matrix ,Mice ,Chondrocytes ,medicine ,Animals ,Chemistry ,Cartilage ,Autophagy ,Cell Biology ,HDAC6 ,In vitro ,Cell biology ,Histone Deacetylase Inhibitors ,Disease Models, Animal ,Oxidative Stress ,osteoarthritis ,medicine.anatomical_structure ,histone deacetylase ,Oxidative stress ,Research Paper - Abstract
The aim of this study was to determine the effect of HDAC6 inhibition using the selective inhibitor Tubastatin A (TubA) on the regulation of tert-butyl hydroperoxide (TBHP)-treated chondrocytes and a mouse OA model. Using conventional molecular biology methods, our results showed that the level of HDAC6 increases both in the cartilage of osteoarthritis (OA) mice and TBHP-treated chondrocytes in vitro. TubA treatment effectively inhibits the expression of HDAC6, attenuates oxidative stress, reduces the level of apoptotic proteins to maintain chondrocyte survival, and suppresses the extracellular matrix (ECM) degradation. In addition, our results also revealed that HDAC6 inhibition by TubA activates autophagy in chondrocytes, whereas the protective effects of TubA were abolished by autophagy inhibitor intervention. Subsequently, the positive effects of HDAC6 inhibition by TubA were also found in a mouse OA model. Therefore, our study provide evidence that HDAC6 inhibition prevents OA development, and HDAC6 could be applied as a potential therapeutic target for OA management.
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- 2021
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17. Cardiopulmonary resuscitation and termination of resuscitation on out-of-hospital cardiac arrest in China
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Jinjun Zhang, Jun Liang, Shengmei Niu, Sijia Tian, Xiao-Jun He, and Yang Liu
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medicine.medical_specialty ,China ,Emergency Medical Services ,Termination of resuscitation ,business.industry ,medicine.medical_treatment ,General Medicine ,Out of hospital cardiac arrest ,Cardiopulmonary Resuscitation ,Emergency medicine ,medicine ,Humans ,Cardiopulmonary resuscitation ,business ,Out-of-Hospital Cardiac Arrest - Published
- 2022
18. Long Noncoding RNA TINCR-Mediated Regulation of Acetyl-CoA Metabolism Promotes Nasopharyngeal Carcinoma Progression and Chemoresistance
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Ying Sun, Ying-Qin Li, Jun Ma, Jia-Li Guan, Rui-Qi Liu, Zhi-Xuan Li, Na Liu, Jia Kou, Fo-Ping Chen, Guan-Qun Zhou, Si-Si Xu, Jun-Yan Li, Jia-Wei Lv, Li Lin, Yue Chen, Xiao-Jun He, Lu-Lu Zhang, Feng Li, Zi-Qi Zheng, and Xu Liu
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0301 basic medicine ,Cancer Research ,RNA Stability ,Antineoplastic Agents ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Acetyl Coenzyme A ,Cell Movement ,Protein-Arginine Deiminase Type 1 ,Cell Line, Tumor ,Lipid biosynthesis ,medicine ,Animals ,Humans ,Gene silencing ,Regulation of gene expression ,Mice, Inbred BALB C ,Nasopharyngeal Carcinoma ,biology ,Ubiquitination ,RNA-Binding Proteins ,Prognosis ,medicine.disease ,Xenograft Model Antitumor Assays ,Long non-coding RNA ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Histone ,Oncology ,Nasopharyngeal carcinoma ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,ATP Citrate (pro-S)-Lyase ,biology.protein ,Cancer research ,RNA, Long Noncoding ,Cisplatin ,Carcinogenesis - Abstract
Frontier evidence suggests that dysregulation of long noncoding RNAs (lncRNA) is ubiquitous in all human tumors, indicating that lncRNAs might have essential roles in tumorigenesis. Therefore, an in-depth study of the roles of lncRNA in nasopharyngeal carcinoma (NPC) carcinogenesis might be helpful to provide novel therapeutic targets. Here we report that lncRNA TINCR was significantly upregulated in NPC and was associated positively with poor survival. Silencing TINCR inhibited NPC progression and cisplatin resistance. Mechanistically, TINCR bound ACLY and protected it from ubiquitin degradation to maintain total cellular acetyl-CoA levels. Accumulation of cellular acetyl-CoA promoted de novo lipid biosynthesis and histone H3K27 acetylation, which ultimately regulated the peptidyl arginine deiminase 1 (PADI1)–MAPK–MMP2/9 pathway. In addition, insulin-like growth factor 2 mRNA-binding protein 3 interacted with TINCR and slowed its decay, which partially accounted for TINCR upregulation in NPC. These findings demonstrate that TINCR acts as a crucial driver of NPC progression and chemoresistance and highlights the newly identified TINCR–ACLY–PADI1–MAPK–MMP2/9 axis as a potential therapeutic target in NPC. Significance: TINCR-mediated regulation of a PADI1–MAPK–MMP2/9 signaling pathway plays a critical role in NPC progression and chemoresistance, marking TINCR as a viable therapeutic target in this disease.
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- 2020
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19. Clinical evaluation of percutaneous endovascular radiofrequency ablation for portal vein tumor thrombus: experience in 120 patients
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Ya-Lin Kong, Jin-Jin Sun, Hong-Yi Zhang, Ying Xing, Cheng Wang, Yang Liu, Xiao-Jun He, Ling-Hong Kong, and Cheng-Li Liu
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Surgery - Abstract
Portal vein tumor thrombosis (PVTT) secondary to primary liver carcinoma (PLC) is commonly associated with poor prognosis and poses great challenge. This study was to evaluate the efficacy and safety of percutaneous endovascular radiofrequency ablation (RFA) in treatment of PVTT.Consecutive patients who were performed endovascular RFA because of PVTT in single-institution in recent 8 years were retrospectively reviewed, compared with patients who underwent only sequential transcatheter arterial chemoembolization (TACE) during the contemporary period. Patency of portal vein, complications, and overall survival (OS) were investigated.One hundred and 20 patients who underwent endovascular RFA and 96 patients who underwent only sequential TACE were included. No severe complications happened in both groups. Except the higher rates of severe fever and moderate pain in the study group, no difference was found in the incidence of side effects and complications. The effective rate in the study group was (78.3%, 94/120) significantly higher than the comparison group (35.4%, 34/96). The median survival time and 1-3 years cumulative survival rates in the study group were 15.7 months and 42.5%, 21.7%, 2.5%, respectively, and 11.3 months, 21.9%, 9.4%, 0 correspondingly in the comparison group, without significant difference. Type of PVTT and Child-Pugh classification of liver function were independent risk factors, and OS was significantly improved by endovascular RFA and subsequent therapy.Endovascular RFA is technically safe and feasible for unresectable PLC and PVTT to improve the prognosis and quality of life.
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- 2022
20. Site‐Selective N ‐1 and C‐3 Heteroarylation of Indole with Heteroarylnitriles by Organocatalysis under Visible Light
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Chao Zhou, Qi‐Chao Gan, Tai‐Ping Zhou, Tao Lei, Chen Ye, Xiao‐Jun He, Bin Chen, Heng Lu, Qian Wan, Rong‐Zhen Liao, Chen‐Ho Tung, and Li‐Zhu Wu
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General Medicine ,General Chemistry ,Catalysis - Abstract
Site-selective N-1 and C-3 arylation of indole has been sought after because of the prevalent application of arylindoles and the intricate reactivities associated with the multiple sites of the N-unsubstituted indole. Represented herein is the first regioselective heteroarylation of indole via a radical-radical cross-coupling by visible-light irradiation. Steady and time-resolved spectroscopic and computational studies revealed that the hydrogen-bonding interaction of organic base and its conjugated acid, namely with indole and heteroarylnitrile, determined the reaction pathway, which underwent either proton-coupled electron-transfer or energy-transfer for the subsequent radical-radical cross-coupling, leading to the regioselective formation of C-3 and N-1 heteroarylation of indoles, respectively. The parallel methodologies for regioisomeric N-1 and C-3 heteroaryl indoles with good functional group compatibility could be applied to large-scale synthesis and late-stage derivatization of bioactive compounds under extremely mild reaction conditions.
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- 2022
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21. What Should We Focus on in Sepsis Fluid Resuscitation? - A Research Based on Scientometrics and Visual Analysis
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Xiao Jun, He, Xiang, Zhu, Yue Feng, Ma, and Jun, Liang
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Sepsis ,Fluid Therapy ,Humans ,Global Health - Published
- 2021
22. A novel local variance-based filtering method for denoising remote sensing images
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Ya Qiong Wang, Yu Li, Xiao Jun He, and Ai Gong Xu
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Image domain ,010504 meteorology & atmospheric sciences ,Computer science ,Noise reduction ,Gaussian ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,0211 other engineering and technologies ,02 engineering and technology ,Variance (accounting) ,01 natural sciences ,Image (mathematics) ,symbols.namesake ,Remote sensing (archaeology) ,Computer Science::Computer Vision and Pattern Recognition ,Local variance ,Earth and Planetary Sciences (miscellaneous) ,symbols ,Electrical and Electronic Engineering ,021101 geological & geomatics engineering ,0105 earth and related environmental sciences ,Remote sensing - Abstract
In this paper, a novel filtering method is designed for denoising remote sensing image. Firstly, the image domain of noisy image is partitioned into blocks for estimating the variance of Gaussian w...
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- 2019
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23. Hand-in-hand quantum dot assembly sensitized photocathodes for enhanced photoelectrochemical hydrogen evolution
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Xiao-Jun He, Chen-Ho Tung, Li-Zhu Wu, Shuai Zhou, Yang Wang, Xu-Bing Li, Ya-Jing Chen, and Hao-Lin Wu
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Photocurrent ,Materials science ,Renewable Energy, Sustainability and the Environment ,business.industry ,Charge (physics) ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Photocathode ,Cadmium telluride photovoltaics ,Quantum dot ,Modulation ,Optoelectronics ,General Materials Science ,Hydrogen evolution ,0210 nano-technology ,Absorption (electromagnetic radiation) ,business - Abstract
Controllable construction of quantum dot (QD)-sensitized photocathodes with simultaneously enhanced light absorption as well as efficient charge separation and oriented migration at the electrode interface is still a big challenge in photoelectrochemical (PEC) hydrogen evolution. Here, for the first time, a hand-in-hand assembly of QDs is introduced to fabricate a co-sensitized photocathode. Due to the complementary visible-light absorption and the typical type-II staggered band-position alignment between CdSe and CdTe QDs, this assembled framework not only integrates functions of the individual constituent QDs in light harvesting, but also realizes efficient charge separation and directional charge migration at the electrode interface. Compared to single-variety QD, physically mixed QD, traditional core/shell QD and sequential deposition QD photocathodes under identical conditions, the assembled system gives a much higher photocurrent density of up to −140 µA cm−2 for H2 evolution from neutral water. This work provides not only a promising toolkit for the sensitization of PEC systems with QD assemblies, but also new insights about photo-carrier modulation for future innovation, especially in establishing highly efficient artificial photosynthetic systems for feasible solar-to-fuel conversion.
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- 2019
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24. Establishing M1 stage subdivisions by incorporating radiological features and Epstein-Barr virus DNA for metastatic nasopharyngeal carcinoma
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Jun Ma, Xiao-Jun He, Huaqiang Zhou, Guan-Qun Zhou, Fo-Ping Chen, Ying Sun, Li Lin, Jia Kou, Xiao-Dan Huang, Wei-Hong Zheng, and Jia-Wei Lv
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,business.industry ,Recursive partitioning ,General Medicine ,medicine.disease ,Virus ,Metastasis ,Lesion ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Radiological weapon ,Internal medicine ,Risk stratification ,Medicine ,Original Article ,Stage (cooking) ,medicine.symptom ,business - Abstract
BACKGROUND: Significance of plasma Epstein-Barr virus deoxyribonucleic acid (EBV DNA)—a proven robust indicator for nasopharyngeal carcinoma (NPC)—is not yet clarified in risk stratification of metastatic NPC (mNPC). We aim to establish effective M1 stage subdivisions in mNPC by integrating radiological features and EBV DNA at diagnosis of metastasis (mEBV DNA). METHODS: The study comprised 1,007 mNPC patients, including 817 metachronous mNPC (mmNPC) patients randomized into training (n=613) and internal validation (n=204) cohorts, and 190 synchronous mNPC (smNPC) patients defined as smNPC validation cohort. Primary clinical end-point was overall survival (OS). Covariate inclusion to recursive partitioning analysis (RPA)-generated risk stratification was qualified by a multivariable two-sided P
- Published
- 2020
25. Competing risk nomograms for nasopharyngeal carcinoma in the intensity-modulated radiotherapy era: A big-data, intelligence platform-based analysis
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Chen-Fei Wu, Jun Ma, Liang Peng, Chen-Wen Zhong, Zi-Qi Zheng, Guan-Qun Zhou, Huaqiang Zhou, Xiao-Dan Huang, Jia-Wei Lv, Ying Sun, and Xiao-Jun He
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Adult ,Big Data ,Male ,0301 basic medicine ,Oncology ,China ,medicine.medical_specialty ,medicine.medical_treatment ,Comorbidity ,Competing risks ,Risk Assessment ,Cohort Studies ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Child ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Retrospective Studies ,Aged, 80 and over ,Nasopharyngeal Carcinoma ,business.industry ,Age Factors ,Nasopharyngeal Neoplasms ,Hematology ,Middle Aged ,Nomogram ,Prognosis ,medicine.disease ,Confidence interval ,Radiation therapy ,Nomograms ,030104 developmental biology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Cohort ,Female ,Radiotherapy, Intensity-Modulated ,Intensity modulated radiotherapy ,business - Abstract
Lacking quantitative evaluations of competing risk data of nasopharyngeal carcinoma (NPC), we aimed to evaluate the probability of NPC- and other cause-specific mortality (NPC-SM; OCSM) and develop competing risk nomograms to quantify survival differences.Using the institutional big-data intelligence platform, 7251 NPC patients undergoing intensity-modulated radiotherapy between 2009-2014 were identified to establish nomograms based on Fine and Gray's competing risk analysis.The 5-year NPC-SM and OCSM of the cohort were 13.1% and 1.2%, respectively, and elevated 5-year OCSMs were observed in patients aged ≥65 years (5.5%) or with severe comorbidities (4.3%). Age was most predictive of OCSM: patients aged 55-64 and ≥65 years exhibited subdistribution hazard ratios (SHRs) of 2.70 (95% confidence interval [CI], 1.64-4.4; P .001) and 5.78 (95% CI, 3.32-10.08; P .001), respectively. Comorbidity measured using the Charlson Comorbidity Index (CCI) was also strongly predictive of OCSM: patients with CCI scores of 1 and ≥2 exhibited SHRs of 2.33 (95% CI, 1.46-3.71; P .001) and 2.58 (95% CI, 1.16-5.73; P = .020), respectively. All validated factors were integrated into the competing nomograms: age, sex, histology type, tumor and node stages, plasma Epstein-Barr virus-DNA level, lactate dehydrogenase level, and C-reactive protein (CRP) level into the NPC-SM model (concordance [c]-index = 0.743); and age, CCI, Albumin level, and CRP level into the OCSM model (c-index = 0.793).OCSM represents a significant competing event for NPC-SM in elderly patients and patients with comorbidities. We present the first prognostic nomograms to quantify competing risks, which may help to tailor individualized treatment.
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- 2018
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26. Emerging blood exosome-based biomarkers for preclinical and clinical Alzheimer’s disease: a meta-analysis and systematic review
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Li-Dian Chen, Wei-Lin Liu, Hua-Wei Lin, Miao-Ran Lin, Yan Yu, Huan-Huan Liu, Ya-Ling Dai, Le-Wen Chen, Wei-Wei Jia, Xiao-Jun He, Xiao-Ling Li, Jing-Fang Zhu, Xie-Hua Xue, and Jing Tao
- Subjects
Developmental Neuroscience - Abstract
Blood exosomes, which are extracellular vesicles secreted by living cells into the circulating blood, are regarded as a relatively noninvasive novel tool for monitoring brain physiology and disease states. An increasing number of blood cargo-loaded exosomes are emerging as potential biomarkers for preclinical and clinical Alzheimer's disease. Therefore, we conducted a meta-analysis and systematic review of molecular biomarkers derived from blood exosomes to comprehensively analyze their diagnostic performance in preclinical Alzheimer's disease, mild cognitive impairment, and Alzheimer's disease. We performed a literature search in PubMed, Web of Science, Embase, and Cochrane Library from their inception to August 15, 2020. The research subjects mainly included Alzheimer's disease, mild cognitive impairment, and preclinical Alzheimer's disease. We identified 34 observational studies, of which 15 were included in the quantitative analysis (Newcastle-Ottawa Scale score 5.87 points) and 19 were used in the qualitative analysis. The meta-analysis results showed that core biomarkers including Aβ
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- 2022
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27. [Untitled]Effect of proprioceptive neuromuscular facilitation on muscular fatigue following 5-km military training and the optimal intervention time
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Xiao-jun, He, Min-min, Ma, Wu-wei, Zhao, Yong, Wang, Min, Zhu, Shang-feng, Gong, Lei, Xia, Wen-jing, Lei, and Hua, Xiao
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- 2011
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28. [Untitled]Effect of proprioceptive neuromuscular facilitation on muscular fatigue following 5-km military training and the optimal intervention time
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Xiao-jun, He, Min-min, Ma, Wu-wei, Zhao, Yong, Wang, Min, Zhu, Shang-feng, Gong, Lei, Xia, Wen-jing, Lei, and Hua, Xiao
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- 2011
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29. Optimal cumulative cisplatin dose in nasopharyngeal carcinoma patients receiving additional induction chemotherapy
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Wen Fei Li, Guan Qun Zhou, Ling Long Tang, Ying Sun, Ai Hua Lin, Jia Wei Lv, Xiao Jun He, Lei Chen, Yan Ping Mao, Jun Ma, Yu Pei Chen, and Zhen Yu Qi
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Male ,0301 basic medicine ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Cancer Research ,Databases, Factual ,Gastroenterology ,0302 clinical medicine ,Nasopharyngeal Carcinoma ,Chemoradiotherapy ,General Medicine ,Middle Aged ,real world data ,Treatment Outcome ,Oncology ,030220 oncology & carcinogenesis ,Female ,Original Article ,medicine.drug ,Adult ,medicine.medical_specialty ,Antineoplastic Agents ,Subgroup analysis ,Disease-Free Survival ,Young Adult ,03 medical and health sciences ,cumulative cisplatin dose ,Clinical Research ,Internal medicine ,medicine ,Carcinoma ,Humans ,induction chemotherapy ,Aged ,Cisplatin ,Dose-Response Relationship, Drug ,intensity‐modulated radiation therapy ,business.industry ,Cancer ,Induction chemotherapy ,Nasopharyngeal Neoplasms ,Original Articles ,equipment and supplies ,medicine.disease ,Confidence interval ,030104 developmental biology ,Nasopharyngeal carcinoma ,Propensity score matching ,business - Abstract
To clarify the optimal cumulative cisplatin dose (CCD) in locoregionally‐advanced nasopharyngel carcinoma (NPC) patients receiving induction chemotherapy (IC) plus concurrent chemoradiotherapy (CCRT). Using the NPC‐specific database from the established big‐data intelligence platform at Sun Yat‐Sen University Cancer Center, 583 non‐disseminated, locoregionally‐advanced NPC patients receiving IC plus CCRT were enrolled. Propensity score matching (PSM) analysis was conducted to control for confounding factors. The median CCD was 160 mg/m2 after IC (range, 40‐300 mg/m2); only 74 patients (12.7%) achieved CCD >200 mg/m2. Patients receiving >200 mg/m2 CCD did not show significantly improved 5‐year overall survival (OS) (HR = 1.19; 95% confidence intervals [CI] 0.69‐2.06, P = .53) and progression‐free survival (PFS) (HR = 1.03; 95% CI: 0.63‐1.68, P = .92) compared with patients receiving 160 mg/m2 and CCD < 160 mg/m2 in both the original and PSM cohorts. In addition, subgroup analysis indicated a favorable PFS, but not OS, with higher cisplatin administration in patients with pretreatment Epstein–Barr virus deoxyribonucleic acid (EBV DNA)
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- 2018
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30. Studies on meat color, myoglobin content, enzyme activities, and genes associated with oxidative potential of pigs slaughtered at different growth stages
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Jian Jun Zuo, Xian Hua Ou, Tao Dong, Ding Yuan Feng, Min Hao Xia, Yi Hua Liu, Xiao Jun He, Hui Ze Tan, Juan Xiao, Shi Geng Zou, Tao Zheng, Fan Wu, and Qin Ping Yu
- Subjects
0301 basic medicine ,lcsh:Animal biochemistry ,Enzyme Activities ,Oxidative phosphorylation ,Biology ,Malate dehydrogenase ,Article ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Animal Products ,Lactate dehydrogenase ,Gene expression ,Food science ,lcsh:QP501-801 ,lcsh:SF1-1100 ,chemistry.chemical_classification ,Meat Color ,Myoglobin ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Enzyme assay ,030104 developmental biology ,Enzyme ,Metmyoglobin ,chemistry ,biology.protein ,Animal Science and Zoology ,Pigs ,lcsh:Animal culture ,Food Science - Abstract
Objective This experiment investigated meat color, myoglobin content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods Sixty 4-week-old Duroc×Landrace×Yorkshire pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (p
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- 2017
31. Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes
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Min Hao Xia, Qin Ping Yu, Fan Wu, Xiao Jun He, Xian Hua Ou, Yi Hua Liu, Tao Dong, Shi Geng Zou, Tao Zheng, Jian Jun Zuo, Hui Ze Tan, and Ding Yuan Feng
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0301 basic medicine ,medicine.medical_specialty ,Myosin ATPase ,lcsh:Animal biochemistry ,macromolecular substances ,Biology ,Article ,03 medical and health sciences ,Animal Products ,Internal medicine ,Psoas major muscle ,Myosin ,Gene expression ,medicine ,Myocyte ,lcsh:QP501-801 ,lcsh:SF1-1100 ,Cell Proliferation ,0402 animal and dairy science ,Skeletal muscle ,04 agricultural and veterinary sciences ,musculoskeletal system ,040201 dairy & animal science ,Muscle Fiber ,Traditional Chinese Medicine Formula ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Real-time polymerase chain reaction ,Pigs ,Animal Science and Zoology ,lcsh:Animal culture ,Skeletal muscle satellite cell proliferation ,Food Science - Abstract
Objective This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula’s extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p
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- 2017
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32. Prognostic potential of liquid biopsy tracking in the posttreatment surveillance of patients with nonmetastatic nasopharyngeal carcinoma
- Author
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Guan-Qun Zhou, Chen-Fei Wu, Zi-Qi Zheng, Xiao-Dan Huang, Dan-Wan Wen, Zhi-Xuan Li, Jia-Wei Lv, Fo-Ping Chen, Yue Chen, Jia Kou, Ying Sun, Xiao-Jun He, and Li Lin
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Databases, Factual ,medicine.medical_treatment ,Disease ,Gastroenterology ,Sensitivity and Specificity ,Virus ,03 medical and health sciences ,0302 clinical medicine ,Interquartile range ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Liquid biopsy ,Neoplasm Metastasis ,Pathological ,Nasopharyngeal Carcinoma ,business.industry ,Incidence ,Liquid Biopsy ,Distant metastasis ,Reproducibility of Results ,Nasopharyngeal Neoplasms ,medicine.disease ,Prognosis ,Radiation therapy ,Oncology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Population Surveillance ,DNA, Viral ,Female ,Neoplasm Recurrence, Local ,business - Abstract
BACKGROUND The current study was performed to investigate whether circulating cell-free Epstein-Barr virus DNA (cfEBV DNA) would be useful for posttreatment surveillance in patients with nasopharyngeal carcinoma (NPC). METHODS The authors identified a total of 1984 nondisseminated NPC patients from an institutional big-data research platform. Blood samples were collected within 3 months of the completion of radiotherapy and every 3 to 12 months thereafter for cfEBV DNA analysis. Patients were followed until disease recurrence was detected or for a median of 60 months. Diagnostic performance was assessed by calculating the sensitivity, specificity, and accuracy based on the clinical detection of disease recurrence by conventional surveillance modalities (imaging scans and pathological examination). RESULTS During follow-up, a total of 767 patients (38.7%) had detectable cfEBV DNA. The recurrence rate among these patients was 63.8% (489 of 767 patients), which was significantly higher than that in patients with undetectable cfEBV DNA (8.6%; 105 of 1217 patients). cfEBV DNA sensitivity, specificity, and accuracy were 68.8%, 80.0%, and 78.2%, respectively, for local recurrence; 80.2%, 80.0%, and 85.9%, respectively, for regional recurrence; and 91.1%, 80.0%, and 92.8%, respectively, for distant metastasis. cfEBV DNA was found to have higher sensitivity for the detection of extrapulmonary metastases (94.9%-96.5%) compared with pulmonary metastases (78.4%). It is interesting to note that among the patients with disease recurrence with detectable cfEBV DNA, positive cfEBV DNA results preceded radiological and/or clinical evidence of disease recurrence by a median of 2.3 months (interquartile range, 0.1-9.5 months). In addition, of the 278 cfEBV DNA-positive patients who did not develop disease recurrence, 227 (81.7%) had transiently positive cfEBV DNA that fell to undetectable levels during long-term monitoring. CONCLUSIONS Plasma cfEBV DNA in patients with NPC appears to be an early sign of tumor recurrence, especially extrapulmonary metastases.
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- 2019
33. T2 Fluid-Attenuated Inversion Recovery Resection for Glioblastoma Involving Eloquent Brain Areas Facilitated Through Awake Craniotomy and Clinical Outcome
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Ming ming Yang, De liu Lin, Zheng hao Fu, Xin qing Deng, Juan Li, Jian kan Lu, Yan feng Fan, Ming Lu, Zhongping Chen, Xiao jun He, You ming Gu, and Ming yao Lai
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Eloquent Brain Areas ,Inversion recovery ,Fluid-attenuated inversion recovery ,Resection ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,medicine ,Humans ,Karnofsky Performance Status ,Wakefulness ,Aged ,medicine.diagnostic_test ,business.industry ,Brain Neoplasms ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,Tumor Burden ,Awake craniotomy ,Treatment Outcome ,030220 oncology & carcinogenesis ,Surgery ,Female ,Neurology (clinical) ,Sodium fluorescein ,Radiology ,business ,Glioblastoma ,030217 neurology & neurosurgery ,Craniotomy - Abstract
Despite evidence that a greater extent of resection (EOR) improves survival, the role of extended resection based on magnetic resonance imaging (MRI) fluid-attenuated inversion recovery (FLAIR) in the prognosis of glioblastoma (GBM) remains controversial. This study aims to investigate the role of additional resection of FLAIR-detected abnormalities and its influence on clinical outcomes of patients with GBM.Forty-six patients with newly diagnosed GBM involving eloquent brain areas were included. Surgeries were performed using awake craniotomy (AC) or AC combined with sodium fluorescein (SF) guidance. Following total removal of the contrast-enhancing tumor area, the EOR of FLAIR abnormalities was dichotomized to identify the best separation threshold for progression-free survival (PFS), overall survival (OS), and 30-day postoperative neurologic function of patients with GBM.The threshold for removal of FLAIR abnormalities affecting survival was determined to be 25%. The median OS and PFS were shorter in the group with FLAIR resection25% compared with the group with FLAIR resection ≥25% (12 months vs. 26 months; P = 0.001 and 6 months vs. 15 months; P = 0.016, respectively). Univariate and multivariate analyses identified tumor location within or near the eloquent brain areas and the 25% threshold for FLAIR EOR as independent factors affecting OS and PFS.Identifying a feasible threshold for the resection of FLAIR abnormalities is valuable in improving the survival of patients with GBM. Extended resection of GBM involving eloquent brain areas was safe when using a combination of AC and SF-guided surgery.
- Published
- 2019
34. Comprehensive characterization of the alternative splicing landscape in head and neck squamous cell carcinoma reveals novel events associated with tumorigenesis and the immune microenvironment
- Author
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Jia Kou, Guan-Qun Zhou, Jun Ma, Jia-Li Guan, Feng Li, Zi-Qi Zheng, Fo-Ping Chen, Xiao-Jun He, Rui-Qi Liu, Ying Sun, Jia-Wei Lv, Zhi-Xuan Li, Zhuo-Hui Wei, Li Lin, Lu-Lu Zhang, and Xiao-Dan Huang
- Subjects
0301 basic medicine ,Male ,Chemokine ,Carcinogenesis ,Medicine (miscellaneous) ,medicine.disease_cause ,head and neck squamous cell carcinoma ,Disease-Free Survival ,Cohort Studies ,03 medical and health sciences ,Splicing factor ,alternative splicing ,0302 clinical medicine ,Immune system ,medicine ,Biomarkers, Tumor ,Tumor Microenvironment ,Humans ,immune microenvironment ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Viral Carcinogenesis ,biology ,Squamous Cell Carcinoma of Head and Neck ,Gene Expression Profiling ,Alternative splicing ,Papillomavirus Infections ,medicine.disease ,Prognosis ,Head and neck squamous-cell carcinoma ,tumorigenesis ,030104 developmental biology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,RNA splicing ,Cancer research ,biology.protein ,Female ,Research Paper ,genome-wide analysis - Abstract
Alternative splicing (AS) has emerged as a key event in tumor development and microenvironment formation. However, comprehensive analysis of AS and its clinical significance in head and neck squamous cell carcinoma (HNSC) is urgently required. Methods: Genome-wide profiling of AS events using RNA-Seq data from The Cancer Genome Atlas (TCGA) program was performed in a cohort of 464 patients with HNSC. Cancer-associated AS events (CASEs) were identified between paired HNSC and adjacent normal tissues and evaluated in functional enrichment analysis. Splicing networks and prognostic models were constructed using bioinformatics tools. Unsupervised clustering of the CASEs identified was conducted and associations with clinical, molecular and immune features were analyzed. Results: We detected a total of 32,309 AS events and identified 473 CASEs in HNSC; among these, 91 were validated in an independent cohort (n = 15). Functional protein domains were frequently altered, especially by CASEs affecting cancer drivers, such as PCSK5. CASE parent genes were significantly enriched in pathways related to HNSC and the tumor immune microenvironment, such as the viral carcinogenesis (FDR < 0.001), Human Papillomavirus infection (FDR < 0.001), chemokine (FDR < 0.001) and T cell receptor (FDR
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- 2019
35. Long Noncoding RNA FAM225A Promotes Nasopharyngeal Carcinoma Tumorigenesis and Metastasis by Acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3
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Zi-Qi Zheng, Lu-Lu Zhang, Jun Ma, Na Liu, Ying-Qin Li, Li Lin, Rui-Qi Liu, Zhi-Xuan Li, Xiao-Jun He, Ying Sun, Xin Wen, Guan-Qun Zhou, Jia-Wei Lv, Xiao-Dan Huang, Fo-Ping Chen, Jian Zhang, and Jia Kou
- Subjects
0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Carcinogenesis ,Mice, Nude ,Apoptosis ,Biology ,medicine.disease_cause ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,otorhinolaryngologic diseases ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Regulation of gene expression ,Mice, Inbred BALB C ,Nasopharyngeal Carcinoma ,Microarray analysis techniques ,Competing endogenous RNA ,Integrin beta3 ,Nasopharyngeal Neoplasms ,medicine.disease ,Prognosis ,Xenograft Model Antitumor Assays ,Long non-coding RNA ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,MicroRNAs ,030104 developmental biology ,Oncology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Cancer research ,Female ,RNA, Long Noncoding ,Transcriptome - Abstract
Long noncoding RNAs (lncRNA) play important roles in the tumorigenesis and progression of cancers. However, the clinical significance of lncRNAs and their regulatory mechanisms in nasopharyngeal carcinogenesis (NPC) are largely unknown. Here, based on a microarray analysis, we identified 384 dysregulated lncRNAs, of which, FAM225A was one of the most upregulated lncRNAs in NPC. FAM225A significantly associated with poor survival in NPC. N(6)-Methyladenosine (m6A) was highly enriched within FAM225A and enhanced its RNA stability. FAM225A functioned as an oncogenic lncRNA that promoted NPC cell proliferation, migration, invasion, tumor growth, and metastasis. Mechanistically, FAM225A functioned as a competing endogenous RNA (ceRNA) for sponging miR-590-3p and miR-1275, leading to the upregulation of their target integrin β3 (ITGB3), and the activation of FAK/PI3K/Akt signaling to promote NPC cell proliferation and invasion. In summary, our study reveals a potential ceRNA regulatory pathway in which FAM225A modulates ITGB3 expression by binding to miR-590-3p and miR-1275, ultimately promoting tumorigenesis and metastasis in NPC. Significance: These findings demonstrate the clinical significance of the lncRNA FAM225A in nasopharyngeal carcinoma (NPC) and the regulatory mechanism involved in NPC development and progression, providing a novel prognostic indicator and promising therapeutic target.
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- 2019
36. Modified Procedures for ALPPS Based on a Risk-Reduced Strategy: Paralleled Clinical Evaluation at Multiple Institutions
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Ying Xing, Cheng Wang, Xu Han, Yalin Kong, Ling-Hong Kong, Hong-yi Zhang, Jiang-Min Chen, Chengli Liu, Xiao-Jun He, and Jie Li
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medicine.medical_specialty ,Carcinoma, Hepatocellular ,Cirrhosis ,liver cirrhosis ,medicine.medical_treatment ,complication ,Portal vein ligation ,survival ,Liver carcinoma ,medicine ,Overall survival ,Hepatectomy ,Humans ,Ligation ,Gastroenterology & Hepatology ,Portal Vein ,business.industry ,Liver Neoplasms ,General Medicine ,medicine.disease ,Surgery ,Liver ,Hepatocellular carcinoma ,liver carcinoma ,Original Article ,ALPPS ,Complication ,business ,Clinical evaluation - Abstract
Purpose We compared the clinical outcomes of modified procedures for associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) based on a risk-reduced strategy with those of classic ALPPS procedures in treating large liver carcinoma. Materials and methods Short-term outcomes, increases in future liver remnant (FLR) and functional FLR (FFLR), and overall survival (OS) were compared between 45 consecutive patients treated with modified ALPPS procedures and 34 patients treated with classic ALPPS procedures. Results Clinical outcomes after the 1st-stage operation markedly improved with the modified procedures. Although the proportions of liver cirrhosis and hepatocellular carcinoma were higher in the modified group, the mortality and incidence of severe complications did not increase. FLR and FFLR hypertrophy at 1 week after the 1st-stage operation were similar in both groups; however, kinetic growth rates in the modified group were lower. OS rates were similar. Conclusion Modified ALPPS procedures could be safely applied to provide long-term survival for patients with liver cirrhosis without sufficient FLR.
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- 2021
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37. Modified Procedures for ALPPS Based on a Risk-Reduced Strategy: Paralleled Clinical Evaluation at Multiple Institutions.
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Ya-Lin Kong, Ying Xing, Jie Li, Cheng-Li Liu, Xiao-Jun He, Cheng Wang, Jiang-Min Chen, Ling-Hong Kong, Xu Han, and Hong-Yi Zhang
- Abstract
Purpose: We compared the clinical outcomes of modified procedures for associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) based on a risk-reduced strategy with those of classic ALPPS procedures in treating large liver carcinoma. Materials and Methods: Short-term outcomes, increases in future liver remnant (FLR) and functional FLR (FFLR), and overall survival (OS) were compared between 45 consecutive patients treated with modified ALPPS procedures and 34 patients treated with classic ALPPS procedures. Results: Clinical outcomes after the 1st-stage operation markedly improved with the modified procedures. Although the proportions of liver cirrhosis and hepatocellular carcinoma were higher in the modified group, the mortality and incidence of severe complications did not increase. FLR and FFLR hypertrophy at 1 week after the 1st-stage operation were similar in both groups; however, kinetic growth rates in the modified group were lower. OS rates were similar. Conclusion: Modified ALPPS procedures could be safely applied to provide long-term survival for patients with liver cirrhosis without sufficient FLR. [ABSTRACT FROM AUTHOR]
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- 2021
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38. Epigenetic repression of long non-coding RNA MEG3 mediated by DNMT1 represses the p53 pathway in gliomas
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Er-Bao Bian, Gang Zong, Chun-Chun Ma, Xiao-Jun He, Jia Li, Bing Zhao, and Hongliang Wang
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DNA (Cytosine-5-)-Methyltransferase 1 ,0301 basic medicine ,Cancer Research ,Down-Regulation ,Apoptosis ,Epigenetic Repression ,Biology ,Epigenesis, Genetic ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Line, Tumor ,Glioma ,medicine ,Humans ,DNA (Cytosine-5-)-Methyltransferases ,Epigenetics ,Promoter Regions, Genetic ,neoplasms ,Cell Proliferation ,Regulation of gene expression ,MEG3 ,Methylation ,DNA Methylation ,medicine.disease ,nervous system diseases ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,DNA methylation ,Cancer research ,RNA, Long Noncoding ,Tumor Suppressor Protein p53 - Abstract
Epigenetic regulation plays a significant role in gliomas. However, how methylation and long non-coding RNA (lncRNA) cooperates to regulate gliomas progression is largely unknown. In this investigation we showed that the downregulation of MEG3 expression due to hypermethylation of MEG3 was observed in gliomas tissues. Treatment of glioma cells with the DNA methylation inhibitor 5-Aza-2'-deoxycytidine (5-AzadC) decreased aberrant hypermethylation of the MEG3 promoter and prevented the loss of MEG3 expression. In addition, DNMT1 was involved in MEG3 promoter methylation, and was inversely correlated with MEG3 expression in gliomas. The inhibition of DNMT1 repressed the proliferation, clone formation, and induced apoptosis in glioma cells. Importantly, the inhibition of DNMT1 contributed to the activation of p53 pathways in gliomas cells. These results suggest that DNMT1-mediated MEG3 hypermethylation caused the loss of MEG3 expression, followed by the inhibition of the p53 pathways in gliomas.
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- 2015
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39. Prognostic Value of Circulating Lipoprotein in Patients with Locoregionally Advanced Nasopharyngeal Carcinoma
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Jia Kou, Xiao-Jun He, Si-Yang Wang, Ji-Jin Yao, Guan-Qun Zhou, Fan Zhang, Wangjian Zhang, Ying Sun, and Wayne R. Lawrence
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Prognostic factor ,Physiology ,Kaplan-Meier Estimate ,Locoregionally advanced ,Gastroenterology ,lcsh:Physiology ,Disease-Free Survival ,lcsh:Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Clinical significance ,In patient ,lcsh:QD415-436 ,Stage (cooking) ,Lipoprotein ,Ratio ,Neoplasm Staging ,Nasopharyngeal Carcinoma ,Receiver operating characteristic ,lcsh:QP1-981 ,business.industry ,Carcinoma ,Curve analysis ,Nasopharyngeal Neoplasms ,Middle Aged ,medicine.disease ,Prognosis ,Lipoproteins, LDL ,030104 developmental biology ,Nasopharyngeal carcinoma ,ROC Curve ,030220 oncology & carcinogenesis ,Area Under Curve ,Multivariate Analysis ,lipids (amino acids, peptides, and proteins) ,Female ,business ,Lipoproteins, HDL ,Prognostic value - Abstract
Background/Aims: Lipoproteins have been reported to be associated with prognosis in various cancers; however, the prognostic value of lipoproteins in patients with nasopharyngeal carcinoma (NPC) remains largely unknown. We aim to asses the role of circulating lipoproteins in locoregionally advanced NPC patients. Methods: Between October 2009 and August 2012, a total of 1,081 patients with stage III-IVB NPC were included in the analysis. Circulating high-density lipoprotein (HDL) and low-density lipoprotein (LDL) are the two key lipoproteins, which were measured at baseline. Receiver operating characteristic (ROC) curve analysis was used to evaluate different cut-off points for lipoproteins. Actuarial rates were performed using Kaplan–Meier methods and the log-rank test. Results: The cutoff points of HDL, LDL, and LDL/HDL ratio were 1.17 mmol/L, 3.75 mmol/L, and 2.73, respectively. At 5 years, high HDL (> 1.17 mmol/L) was significantly associated with better overall survival (OS, 86.6% vs. 78.9%; P=0.004), distant metastasis-free survival (DMFS, 86.9% vs. 80.8%; P=0.004), locoregional relapse-free survival (LRFS, 90.8% vs. 85.4%; P=0.010), and progression-free survival (PFS, 79.1% vs. 70.2%; P= 0.001) than low HDL (≤1.17 mmol/L). In contrast, high LDL (> 3.75 mmol/L) tend to be inferior OS (79.1% vs. 84.9%; P= 0.016) in compassion with low LDL (≤3.75 mmol/L). Likewise, patients with high LDL/HDL ratio (> 2.73) tend to be inferior OS (79.3% vs. 86.9%; P=0.001), DMFS (81.9% vs. 86.5%; P=0.030), and PFS (72.6% vs. 77.8%; P= 0.034) than those of low LDL/HDL ratio (≤2.73). In multivariate analysis, baseline HDL was found to be a significant prognostic factor for LRFS (HR= 0.65; 95% CI, 0.45-0.93; P= 0.019) and PFS (HR=0.75; 95% CI, 0.58-0.98; P= 0.034). Conclusions: Circulating HDL is significantly associated with treatment outcomes in patients with locoregionally advanced NPC. We suggest that HDL measurements will be of great clinical significance in the management of NPC.
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- 2018
40. Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2
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Chun‑Chun Ma, Er‑Bao Bian, Hong‑Liang Wang, Xiao‑Jun He, Bing Zhao, and Chao Wang
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0301 basic medicine ,Cancer Research ,Gene knockdown ,Cell growth ,Cell ,SKA2 ,RNA ,Articles ,Biology ,Cell cycle ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Downregulation and upregulation ,Glioma ,medicine ,Cancer research - Abstract
The long non-coding RNA SPRY4-intronic transcript 1 (SPRY4-IT1) has been shown to promote the progression of cancer; however, the role of SPRY4-IT1 in glioma remains unclear. The present study demonstrated that SPRY4-IT1 expression was markedly increased in glioma tissues and cells compared with normal brain tissues, whereas knockdown of SPRY4-IT1 inhibited cell proliferation, migration, and invasion in U251 cells. Spindle and kinetochore associated complex subunit 2 (SKA2) was found to be a target of SPRY4-IT1 and was downregulated by SPRY4-IT1-knockdown. Additionally, SPRY4-IT1 expression was positively correlated with SKA2 in glioma tissues. To the best of our knowledge, the present study provides the first demonstration that SKA2 may have an oncogenic role in U251 cells. These results indicate that SPRY4-IT1 may serve a notable role in the molecular etiology of glioma and represents a potential target in glioma therapy.
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- 2018
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41. Development of a Clinicomolecular Risk Stratification System for Non-Metastatic Nasopharyngeal Carcinoma Using Epstein-Barr Virus DNA and TNM Stage in 9,160 Endemic Cases from Southern China
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Zi-Qi Zheng, Xiao-Jun He, Jun Ma, Xin-Yu He, Chen-Fei Wu, Ying Guo, Bao Ting Zhu, Kuan Rui Lloyd Tan, Jia-Wei Lv, Guan-Qun Zhou, Lu-Lu Zhang, Fo-Ping Chen, Li Lin, Melvin L.K. Chua, Jia Kou, Xiao-Dan Huang, Ying-Shan Luo, and Ying Sun
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Oncology ,medicine.medical_specialty ,Proportional hazards model ,business.industry ,medicine.medical_treatment ,Medical record ,Hazard ratio ,medicine.disease ,Radiation therapy ,Nasopharyngeal carcinoma ,Informed consent ,Internal medicine ,Cohort ,medicine ,Stage (cooking) ,business - Abstract
Background: To construct a clinicomolecular index integrating circulating Epstein-barr virus DNA (cfEBV DNA) with T- and N- categories for prognostication in nasopharyngeal carcinoma (NPC). Methods: We utilised medical records of 9,160 biopsy-proven, non-metastatic NPC cases who underwent intensity-modulated radiotherapy (IMRT) with or without chemotherapy. Decision tree modeling (DTM) and adjusted hazard ratio (AHR) methods were used to generate risk models incorporating T- and N-categories and pre-treatment cfEBV DNA load. Predictive accuracy and discrimination of the models were compared against UICC/AJCC 8th edition TNM stage and a RPA-based model. Cox regression was used for survival outcome analyses. Findings: We observed linearity between cfEBV DNA and DFS; cfEBV DNA of >2,000 copies was consistent for risk discretisation (HR >1·0) for DFS, OS and DMF in our cohort of 9,160 patients. DTM and AHR modelling using a two-tiered stratification by cfEBV DNA (≤2,000 and >2,000 copies) and Tand N- categories yielded six RGs with significantly disparate DFS (P
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- 2018
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42. Patient- and treatment-related risk factors associated with neck muscle spasm in nasopharyngeal carcinoma patients after intensity-modulated radiotherapy
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Xiao Jun He, Ying Sun, Ai Hua Lin, Guan Qun Zhou, Jun Ma, Jia Xiang Li, Lu-Lu Zhang, Ling Long Tang, Zhen Yu Qi, and Yan Ping Mao
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Adult ,Male ,Spasm ,Cancer Research ,medicine.medical_specialty ,Prognostic variable ,Intensity-modulated radiotherapy ,medicine.medical_treatment ,Nasopharyngeal neoplasm ,lcsh:RC254-282 ,030218 nuclear medicine & medical imaging ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Neck Muscles ,Risk Factors ,Nasopharyngeal carcinoma ,otorhinolaryngologic diseases ,Genetics ,medicine ,Carcinoma ,Clinical endpoint ,Humans ,Young adult ,Neoplasm Staging ,business.industry ,Incidence ,Incidence (epidemiology) ,Neck muscle spasm ,Dose tolerance ,Nasopharyngeal Neoplasms ,Radiotherapy Dosage ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Surgery ,Radiation therapy ,stomatognathic diseases ,ROC Curve ,Oncology ,030220 oncology & carcinogenesis ,Female ,Radiotherapy, Intensity-Modulated ,business ,Research Article - Abstract
Background To evaluate the incidence of neck muscle spasm in nasopharyngeal carcinoma (NPC) patients that received intensity-modulated radiotherapy (IMRT), and to analyse the patient- and treatment-related risk factors associated with neck muscle spasm. Methods A sample of 152 IMRT-treated, biopsy-proven, nondisseminated NPC patients were retrospectively analysed. All had documented IMRT treatment plans and had returned for follow-up review at 4 years post-radiotherapy. Spasm of the sternocleidomastoid (SCM) muscle was graded from 0 to 3 (absent to severe) and this grade served as the clinical endpoint. Risk factors were identified using logistic regression analysis. Results Within 4 years of radiotherapy, neck muscle spasm developed in 23.68% of the patients; Grades 0, 1, 2 and 3 were respectively assigned to 83.55, 7.57, 6.58 and 2.30% of assessed SCMs. Multivariate analysis indicated that gender, N stage, V60 (percentage of SCM volume that received >60 Gy) were independent prognostic variables, and that the optimal threshold for using V60 to predict neck muscle spasm was 61.92% (sensitivity = 0.900, specificity = 0.953). Conclusions Gender, N stage and V60 were independent predictive factors for post-radiotherapy neck muscle spasm, and a V60 of ≤61.92% in the SCM was relatively safe.
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- 2017
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43. [Clinical observation of the second operation for refracture after internal fixation of clavicle]
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Hong-Wei, Xu, Xiao-Jun, He, Zhong-Wei, Zhang, and Gang, Chen
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Adult ,Fracture Healing ,Male ,Reoperation ,Middle Aged ,Clavicle ,Fracture Fixation, Intramedullary ,Fracture Fixation, Internal ,Fractures, Bone ,Young Adult ,Recurrence ,Humans ,Female ,Bone Plates - Abstract
To evaluate the outcome of Titanium Elastic intramedullary nailing(TEN) for the treatment of refracture of clavicle.From March 2010 to March 2016, 9 cases of clavicle fracture, postoperative re-fracture (plate broken before fracture healing, plate and screw pull out or refracture after fracture union and internal fixation removal) included 6 males and 3 females with a mean age of 38.6 years old ranging from 20 to 62 years old; 3 cases were sports injury, 5 cases were traffic injury, 1 case was severe injury. All cases were closed fractures, and 2 cases were multiple fractures. According to OTA(Orthopaedic Trauma Association classification), 2 cases were 06-A1, 1 case was 06-A2, 1 case was 06-A3, 2 cases were 06-B1, 2 cases were 06-B2, and 1 case was 06-B3. Among them, 4 cases were treated with AO titanium elastic intramedullary nail (TEN), and 5 cases of early internal fixation failure of clavicle fracture were treated with plate and screw internal fixation plus bone graft. The time of bone union, VAS score and Constant Shoulder Score were observed and recorded.All incisions healed at stage I without infection. All 9 patients were followed up for a period of 14 months. All patients' implants were removed at 12 months after the second operation without refractures. The Constant score of shoulder joint was from 64 to 94 at 4 weeks after the second operation, 91 to 100 at 4 weeks after implants removal. The average bone healing time was 4.3 months from 3 to 6 months. The pain VAS score decreased significantly and the pain was relieved significantly.Titanium elastic intramedullary nail (TEN) for the treatment of clavicle fracture after internal fixation of refracture patients can be achieved better outcomes, less trauma, good fracture healing, good postoperative recovery of shoulder joint function, but need to pay attention to the reasonable selection of patients.
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- 2017
44. Inhibition of progression of PanIN through antagonizing EGFR
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Mei Xiao, Hong-yi Zhang, Xiao-Jun He, Hui Zhang, Yalin Kong, and Wenbing Li
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Male ,Oncology ,medicine.medical_specialty ,Pancreatic ductal adenocarcinoma ,endocrine system diseases ,Pancreatic Intraepithelial Neoplasia ,Antineoplastic Agents ,Mice, Transgenic ,Internal medicine ,medicine ,Animals ,Egfr signaling ,Epidermal growth factor receptor ,Pancreas ,EGFR inhibitors ,biology ,business.industry ,Carcinoma in situ ,General Medicine ,Precancerous lesion ,Tyrphostins ,medicine.disease ,digestive system diseases ,ErbB Receptors ,Pancreatic Neoplasms ,medicine.anatomical_structure ,Disease Progression ,Quinazolines ,biology.protein ,Drug Screening Assays, Antitumor ,business ,Carcinoma in Situ - Abstract
Pancreatic ductal adenocarcinoma (PDAC) is an extremely malignant tumor with high lethality in humans. Pancreatic intraepithelial neoplasia (PanIN) is the predominant precancerous lesion for PDAC. Although PanIN is frequently detected in the normal and inflamed pancreas, only a few of PanIN eventually progress into PDAC. Thus, inhibition of PanIN-to-PDAC conversion is critical for preventing the occurrence of PDAC. Here, we evaluated the effect of inhibition of epidermal growth factor receptor (EGFR) signaling on the progression of low-grade PanIN into high-grade PDAC in an established mouse PDAC model (Ptf1a-Cre; K-rasG12D). We found that intraductal infusion of EGFR inhibitors at 12 weeks of age, which induced sustained inhibition of EGFR signaling in the pancreas, significantly decreased the incidence of high-grade PanIN in these mice at 24 weeks of age. Thus, our study suggests that inhibition of EGFR signaling may prevent development of PDAC.
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- 2014
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45. Interactions of Octahedral Ruthenium(II) Polypyridyl Complexes with the RNA Triplex poly(U)•poly(A)*poly(U) Effect on the Third-Strand Stabilization
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Li-Feng Tan and Xiao-Jun He
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Poly U ,RNA Stability ,Circular dichroism ,Molecular Structure ,Polymers ,Pyridines ,Stereochemistry ,Chemistry ,Phenanthroline ,Hoogsteen base pair ,RNA ,chemistry.chemical_element ,Ruthenium ,Inorganic Chemistry ,chemistry.chemical_compound ,Octahedron ,Coordination Complexes ,Quantum Theory ,Thermodynamics ,Molecule ,Physical and Theoretical Chemistry ,Poly A - Abstract
Stable triplexes play key roles in many biological processes. Due to the Hoogsteen base pairing, triplexes are, however, thermodynamically less stable than the corresponding duplexes. The poor stabilization of these structures limits their practical applications under physiological conditions. To understand the factors effect on the stabilization of RNA triplexes by octahedral ruthenium(II) complexes, the interactions of [RuL2(uip)](2+) {where L = 2,2'-bipyridine (bpy) or 1,10-phenanthroline phen, uip = 2-(5-uracil)-1H-imidazo[4,5-f][1,10]phenanthroline} with the RNA triplex poly(U)•poly(A)*poly(U) are examined by spectrophotometry, spectrofluorometry, circular dichroism, and viscosimetry in this work. The main results obtained here suggest that the third-strand stabilization depends on the hydrophobicity effects of ancillary ligands bpy and phen.
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- 2014
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46. Development of Detection Research on Fatigue Driving
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Yuan Quan Yang, Jing Liu, Zhen Di Yi, and Xiao Jun He
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Information fusion ,Engineering ,business.industry ,Fatigue testing ,General Medicine ,business ,Simulation ,Reliability engineering - Abstract
This paper presents the current most common fatigue-driving detection methods. The advantages and disadvantages of these detection methods are compared with. Moreover, several major products of the current fatigue detection are listed briefly. Furthermore, the development trends of driving-fatigue detection technology are prospected. The author believes that driver fatigue testing standards need to be further clarified and the non-contact detection method of driving-fatigue needs to be developed deeply. Information fusion is an important orientation for driving fatigue and we should design the cost-efficient detection products for fatigue-driving.
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- 2014
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47. Defect Detecting Technology Based on Machine Vision of Industrial Parts
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Xiao Jun He, Jing Liu, Yu Zheng Wang, and Zhen Di Yi
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Engineering ,Noise ,Pixel ,Machine vision ,business.industry ,Median filter ,Computer vision ,General Medicine ,Artificial intelligence ,business ,Edge detection ,Image (mathematics) - Abstract
In order to reach and test the surface defects on industrial parts, based on Machine Vision this paper put forward a defective parts detection method. The method of median filter was adopted to eliminate the noise of image. The Ostu-method was used for the segmenting threshold. Pixel level and level edge detection were used to complete the precise components defects detection. Experiments show that this scheme is feasible, and can achieve high accuracy and shorter testing time.
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- 2014
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48. Study of Induction Heating Power Supply Based on 74HC4046
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Qing Bin Chen, Yi Wen Lin, Ze Ping Lin, Yan Cui, Jun Wang, Wei Hao Yang, and Xiao Jun He
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Engineering ,Induction heating ,business.industry ,General Engineering ,Process (computing) ,Tracking system ,Power (physics) ,Control theory ,Control system ,Electronic engineering ,Inverter ,Phase compensation ,business ,TRACE (psycholinguistics) - Abstract
According to the change of resistivity and magnetic permeability of iron load which will influence the resonance frequency in the process of induction heating, this paper presents a frequency tracking system based on the 74HC4046. It can control the output frequency of the power supply in real time, and make the power output frequency to trace the resonance frequency of load. This paper also analyses the phase compensation of the control system, and proposes a new way of phase compensation. The corresponding simulation experiment is done finally. It provides the theory basis to analyze and design an inverter power supply.
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- 2014
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49. Multi-Factor Model Simulation on Dynamics of Population Movements
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Zhen Hu Du, Xiao Jun He, and Jin Feng Du
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Dynamic simulation ,Population migration ,education.field_of_study ,Computer simulation ,Flow (mathematics) ,Computer science ,Process (engineering) ,Urbanization ,Population ,Econometrics ,General Medicine ,Set (psychology) ,education - Abstract
Based on PN junction population, flow model is set up to consider the amount of land to simulate the population migration, and take into account the level of economic development, the factors combine and incorporated into the analytical framework of a model derived the probability of a combination of multiple factors under conditions of population migration. The results of this simulation model can effectively explain the process of urbanization of the population flow problems, is an innovative model generality, there is some reference in the theoretical study.
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- 2014
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50. Damage of rat liver tissue caused by repeated and sustained +Gz exposure and the mechanism thereof
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Wen-bing LI, Ya-lin KONG, Xiao-jun HE, Gang ZHAO, Huai-cheng ZHU, Shen HU, Lei LIU, and Hong-yi ZHANG
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JNK mitogen-activated protein kinases ,lcsh:R5-920 ,genes, jun ,lcsh:R ,lcsh:Medicine ,acceleration ,wounds and injuries ,liver ,lcsh:Medicine (General) - Abstract
Objective To explore the mechanisms of positive acceleration (+Gz) on the damage of rat liver tissue and the effect of +Gz on the expression of JNK/c-Jun in liver cells. Methods Twenty four male Wistar rats were randomly divided into 4 groups (n=6): control, +2Gz, +6Gz and +10Gz group. With prone position, the rats in control group were fixed to the turning arm of centrifuge with head towards the axis for 5 minutes. The fixation method in +2Gz, +6Gz and +10Gz group was the same as in the control group. The increase rate of acceleration was 1G/s with a peak-time of 3 minutes, and each +Gz exposure repeated 5 times with an interval of 30 minutes. HE staining was used to observe the morphological changes of liver tissue, fluorescence real-time quantitative PCR to detect the expression of hepatic c-Jun mRNA, and Western blotting to detect the hepatic protein expression of p-c-Jun, c-Jun, p-JNK and JNK. Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined. Results The levels of serum ALT and AST increased significantly in +6Gz and, especially, the +10Gz group than in control group and +2Gz group (P
- Published
- 2014
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