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ZrO2 nanotube carrier was obtained using a direct anodization method. Then SO42-/ZrO2 nanotube catalyst was developed in the sulfuric acid solution through ultrasonic dipping processing. The morphology, thermal stability and crystal forms of catalysts were analyzed, and the influence factors of catalytic activity were studied. The results presented in this work show that the catalyst activity was highest under the following conditions: the mass fraction of sulfuric acid solution was 50 %, the ultrasonic dipping time was 20 min, the suitable calcination temperature was 400 °C and the calcination time was 60 min.

Yb/Ho co-doped yttria transparent ceramics, Yb/Ho:Y 2 O 3 , were prepared by vacuum sintering with additional 0.5 wt.% tetraethyl orthosilicate (TEOS) as sintering aid from the Yb/Ho co-doped yttria nanopowders synthesized by co-precipitation method. The optical quality of transparent ceramics was improved significantly, as increasing the sintering temperature from 1800 °C to 1850 °C as well as the holding time from 20 h to 25 h, respectively. The absorption spectrum was measured to find out the advisable emission source wavelength. The 2 μm emission bands were observed under laser diode excitation at 980 nm in the co-doped sample with 5 mol% of Yb 3+ and 1 mol% of Ho 3+ , and broad emission bandwidth were obtained.

Advanced oxidation protein products (AOPP) as a biomarker of oxidative stress has been demonstrated in chronic kidney disease (CKD) patients; however, current methods to detect the accumulation of AOPP in serum and in tissues are limited and unreliable. This study generated a monoclonal antibody (mAb) designated 3F2, that reacts specifically with hypochlorous acid (HOCl)-modified proteins, but not with the native forms or with other types of oxidative modifications. Notably, mAb 3F2 recognizes the AOPP deposited in renal tissues of AOPP-treated rats and of patients with different kinds of CKD. Moreover, this mAb can almost completely inhibit the production of reactive oxygen species in RAW264.7 cells induced by AOPP (p < 0.001). In conclusion, mAb 3F2 can be used to detect AOPP specifically in serum and in tissues, and this antibody can potentially provide an important tool and new insight into research on diseases related to oxidative stress.

To screen peptide mimics of PSP (Paralytic shellfish poisoning) GTX2,3 from a random 12-mer phage display peptide library and to identify and characterize the specificity and accuracy of the peptide mimotope of GTX2,3.The monoclonal antibody against GTX2,3 (mAb E(9);F(10);) was used as a target to screen the 12-mer phage display peptide library and the specificity of phage clones were identified by sandwich ELISA and blocking assay. The peptide sequences of positive phage clones were determined and analyzed by DNA sequencing. The affinity and specificity of synthetic peptide were identified by a competitive ELISA.The 20 clones were identified to be specific reactivity with the mAb E(9);F(10);. Amino acid sequence analysis revealed seven different types of mimotope sequence, most of which contained a common motif DXLXPP(X presents random acid amino), X was random amino acid. The Phage No.2(phage 2), the clone with mimotope sequence WPSLDXLXPPSY showed the strongest binding, and inhibited the reactivity of the mAb with GTX2,3. Another ELISA result showed that synthetic peptide-1 (SP-1) which contain mimotope amino acid sequence was able to inhibit the mAb- GTX2,3 interaction.The mimotope peptide of GTX2,3 is obtained by using the phage display technology. The results also showed that SP-1 bind to mAb E(9);F(10); at the same site as GTX2,3.