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Biological invasion is a hot topic in ecological research. Most studies on the physiological mechanisms of plants focus on leaves, but few studies focus on stems. To study the tolerance of invasive plant (Sphagneticola trilobata L.) to low temperature, relevant physiological indicators (including anthocyanin and chlorophyll) in different organs (leaves and stems) were analyzed, using a native species (Sphagneticola calendulacea L.) as the control. The results showed that, upon exposure to low temperature for 15 days, the stems of two Sphagneticola species were markedly reddened, their anthocyanin content increased, chlorophyll and chlorophyll fluorescence parameters decreased, and the accumulation of reactive oxygen species in the stem increased. The percentage increases of antioxidants and total antioxidant capacities in stems were significantly higher in S. trilobata than in S. calendulacea. This showed that S. trilobata had higher cold tolerance in stems while leaves were opposite. To further verify the higher cold tolerance of the stem of S. trilobata, a defoliation experiment was designed. We found that the defoliated stem of S. trilobata reduced anthocyanin accumulation and increased chlorophyll content, while alleviating membrane lipid damage and electrical conductivity, and the defoliated stem still showed an increase in stem diameter and biomass under low temperature. The discovery of the physiological and adaptive mechanisms of the stem of S. trilobata to low temperature will provide a theoretical basis for explaining how S. trilobata maintains its annual growth in South China. This is of great significance for predicting the future spread of cloned and propagated invasive plants.

Climate change and invasive alien species threaten biodiversity. High temperature is a worrying ecological factor. Most responses of invasive plants aimed at coping with adversity are focused on the physiological level. To explore the molecular mechanisms underlying the response of an invasive plant (Sphagneticola trilobata L.) to high temperature, using a native species (Sphagneticola calendulacea L.) as the control, relevant indicators, including photosynthetic pigments, gas exchange, chlorophyll fluorescence, the antioxidant system, and related enzyme-coding genes were measured. The results showed that the leaves of S. calendulacea turned yellow, photosynthetic pigment content (Chl a, Chl b, Car, Chl) decreased, gas exchange (Pn) and chlorophyll fluorescence parameters (Fv/Fm, &Phi, PSII) decreased under high temperature. It was also found that high temperature caused photoinhibition and a large amount of ROS accumulated, resulting in an increase in MDA and relative conductivity. Antioxidant enzymes (including SOD, POD, CAT, and APX) and antioxidants (including flavonoids, total phenols, and carotenoids) were decreased. The qPCR results further showed that the expression of the PsbP, PsbA, and RubiscoL, SOD, POD, CAT, and APX genes was downregulated, which was consistent with the results of physiological data. Otherwise, the resistance of S. trilobata to high temperature was better than that of S. calendulacea, which made it a superior plant in the invasion area. These results further indicated that the gradual warming of global temperature will greatly accelerate the invasion area of S. trilobata.

The aim of the present study was to investigate the molecular mechanism associated with the traditional Chinese medicine formula Gui Zhu Yi Kun formula (GZYKF), in the treatment of polycystic ovary syndrome (PCOS). In this study, granulosa cells (GCs) of rats with PCOS were cultured and treated with testosterone propionate (TP) alone or with serum from rats treated with different doses of GZYKF. The effect of TP on cell growth was assayed using the MTT method. Expression levels of Beclin-1, light chain (LC)3, mechanistic target of rapamycin (mTOR), tumor suppressor p53 (p53), adenosine monophosphate-activated protein kinase (AMPK), sestrin2 and tuberous sclerosis protein 1/2 were evaluated using quantitative polymerase chain reaction and western blotting. It was demonstrated that TP increased the expression of Beclin-1 and LC3, whereas GZYKF significantly decreased the TP-induced expression of Beclin-1 (P

Keloid is a pathologic fibro-proliferative disorder and is characterized by hyper-proliferation of fibroblasts and excess extracellular matrix (ECM) deposition. Interferon regulatory factor 3 (IRF3) is a member of the interferon-regulatory factor (IRF) family and has been shown to play a critical modulator in the progression of fibrosis. However, the function of IRF3 in dermal fibrosis remains unclear. Thus, in this study, we investigated the effects of IRF3 on keloid-derived fibroblasts (KFs) proliferation and ECM expression, and explored the underlying mechanism. Our results indicated that the expression of IRF3 was highly expressed in human keloid tissues. Down-regulation of IRF3 significantly inhibited KF proliferation and the expression of type I collagen and α-smooth muscle actin (α-SMA), as well as suppressed the expression of TGF-β receptor I and II in TGF-β1-stimulated KFs. Furthermore, down-regulation of IRF3 suppressed the phosphorylation levels of Smad2 and Smad3 in human KFs induced by TGF-β1. Taken together, our data showed that down-regulation of IRF3 inhibited the proliferation and ECM expression in KFs via suppressing the TGF-β1/Smad signaling pathway. Thus, our findings suggest that IRF3 may represent a promising target for treatment of the keloid disease.

In clothing modeling, clothing accessories add the finishing touch, and have collection value. With the development of society, people have more demands for accessaries on the style, manufacturing methods, material decoration and other aspects; in the process of seeking innovation on apparel accessory design and craft, the two traditional craftwork of lacquer art and ceramic art is applied—the unique attributes, materials and craftsmanship of lacquer art, and the unique texture and shaping techniques of ceramic art; they create a unique visual beauty for the field of clothing accessories and open up new ideas.

To identify microRNAs (miRNAs) directly regulating the proto-oncogene Bmi-1 expression in the development of hepatocellular carcinoma (HCC) and to explore the underlying molecular mechanisms.Four HCC cell lines, including HepG2, Bel7404, Huh7, and PLC5, the normal hepatocellular cell line MIHA, and 30 HCC biopsies were included in this study. Potential miRNAs, which interact with Bmi-1 and are involved in the development of HCC were identified through bioinformatic analyses. The expression of miRNA and Bmi-1 in HCC cell lines and HCC tissues was analyzed using fluorescence protein analysis, real-time quantitative PCR (RT-qPCR), and Western blotting.Bioinformatic analysis suggested that miR-218 is a potential miRNA regulating Bmi-1 expression. Fluorescence protein analysis, RT-qPCR, and Western blotting confirmed the direct interaction between miR-218 and Bmi-1. In addition, increased expression of Bmi-1 was detected in HCC cell lines and HCC tissues. In most HCC tissues, the expression of miR-218 was decreased and was associated with increased expression of Bmi-1.miR-p218 downregulates the expression of the proto-oncogene Bmi-1 in HCC, and it may be an effective target for the treatment of this disease.

Clothing fabrics, clothing styles, clothing structure design, material texture re-creation need the innovation and re-creation ability of designers. Design sketch is the "window" of designer's personal style, is a special symbol and form of expression and is the basic approach of modeling and the form aesthetic training. In order to better serve the clothing and textile industry in Fujian Province, in this paper, taking the requirement of the clothing industry development in Fujian Province on the designer’s ability as the background, the application and promotion of design sketch in the cultivation of the ability of fashion designers are explored. Fujian Province is located in the hinterland of the economic zone on the west side of the strait and is known for light industry. Its southern Xiamen, Shishi, Jinjiang, Quanzhou, Zhangzhou, its central Putian, Fuzhou, Changle, its northern Sanming, Nanping and other places have large-scale clothing and textile industry which occupies a pivotal position in the local economy. Textile and clothing industry as the traditional processing industry of advantage in Fujian Province, has now formed a complete range of textile industry system involving the categories of chemical fiber, cotton, clothing, printing and dyeing, knitting, industrial textiles, home textiles and so on. Its textile economy is in the forefront in regional economic ranking. For example, in Shishi and Jinjiang which are the national textile industry leaders, the textile industry has become the pillar industry of local economy; Quanzhou in southern Fujian and Changle in central Fujian have built a relatively complete textile industry chain covering the cotton and chemical fiber, weaving and dyeing, clothing and accessories, etc. They occupy a large proportion in the local economy and have become the key of the local economic development.

This study aims to search for a new, economic, convenient, and low recurrence rate operation for the surgical management of pelvic organ prolapse (POP). The clinical value of the operation for treating POP was determined through retrospective case series. The new operation was called, pelvic autologous tissue reconstruction. Women with symptomatic uterine prolapse, who required surgery, were recruited. A total of 97 women [stage III to IV, according to POP quantification (POP-Q) staging] were collected from January 2010 to December 2016. Among these women, 61 women underwent a traditional operation (TO, vaginal hysterectomy and vaginal anterior and posterior wall repair), while the remaining women underwent pelvic autologous tissue reconstruction. First, there was no statistically significant difference in intraoperative blood loss, indwelling urethral catheter time, in-hospital time, and the time of passage of gas through the anus between the pelvic autologous reconstruction (PAR) and TO groups (P > .05). The average operation time in the PAR group was significantly longer than that in the TO group (P .05). Furthermore, BND increased in the TO group at 12 months after the operation, compared with that at 3 months after the operation (P .05). In addition, BND was significantly smaller in the PAR group than in the TO group at 3 and 12 months after the surgery (P

To explore the expression of Rictor and mTOR in the colorectal cancer and their clinical significance.The expression levels of Rictor and mTOR in HCT116, SW480, LoVo and HCoEpiC cells were detected by indirect immunofluorescence and Western blotting. Sixty-two paraffin-embedded surgical specimens of colorectal cancer tissue and adjacent tissues were examined for Rictor expression using immunohistochemistry. The association of the expression levels of Rictor protein with the clinicopathologic features and the overall survival of the patients was analyzed.The expression level of Rictor was significantly higher in colorectal cancer tissues than in the adjacent tissues (P0.05). The expression levels of Rictor and mTOR in the colon cancer cell lines were higher than those in human normal colon epithelial cell line HCoEpiC. The expression of Rictor was correlated with Dukes stage and lymphatic metastasis of the tumors but not with other clinicopathological parameter (P0.05). Patients with Rictor expression had a lower overall survival rate than those without Rictor expression.Rictor overexpression is associated with the carcinogenesis and progression of colorectal cancer and can be an independent indicator for evaluating the prognosis of colorectal cancer patients.

To observe the effect of Bushen Wenyang Huayu Recipe (BWHR) on hypoxia inducible factor-1α (HIF-1α), proline hydroxylase2 (PHD2), von Hippel Lindau disease (VHL) suppressor gene expressions in endometriosis (EM) rats with Shen yang deficiency blood stasis syndrome (SYDBSS), and to explore the pathogenesis of EM and the mechanism of BWHR for treating EM.Totally 50 SD rats were randomly divided into five groups, i.e., the blank control group, the sham-operation group, the model group, the Chinese medicine (CM) group, and the Western medicine (WM) group, 10 in each group. Rats in the blank control group and the sham-operation group were fed routinely. Rats in the rest 3 groups received 30-day "extended refrigerator freezing and ice water immersion" and combined with " autotransplantation" to establish EM rat model with SYDBSS. One Milliliter BWHR at 3.33 g/mL was administered to rats in the CM group by gastrogavage. Gestrinone at the daily dose of 0. 5 mg/kg was administered to rats in the WM group by gastrogavage. Equal volume of normal saline was administered to rats in the model group, the blank control group, and the sham-operation group. The size and morphology of ectopic foci in rats were observed after 4 weeks of medication. Expressions of serum CA125, plasma cyclic adenosine monophosphate (cAMP), and plasma cyclic guanosine monophosphate (cGMP) were detected by radioimmunoassay. Morphological changes of eutopic endometrium and ectopic tissue were observed under the optical microscope by HE staining. Protein expressions and contents of HIF-lα, PHD2, and VHL were detected by immunohistochemical SABC method and Western blot. mRNA expressions of HIF-1α, PHD2, and VHL were detected by RT-PCR.The ectopic foci grew significantly in the model group. Their volumes were obviously contracted after treated by CM and WM. Compared with the blank control group and the sham-operation group, serum CA125 and plasma cGMP obviously increased, cAMP obviously decreased (P0.05); expressions and contents of HIF-1α mRNA and protein all decreased (P0.05); mRNA and protein expressions and contents of PHD2 and VHL all decreased in the model group (P0.05). Compared with model group, levels of CA125 and cGMP obviously decreased; cAMP levels obviously increased, expressions and contents of HIF-1α mRNA and protein all increased, mRNA and protein expressions and contents of PHD2 and VHL all increased in the WM group and the CM group (P0.05). Compared with the CM group, PHD2 protein contents were higher in the WM group (P0.05). HIF-1α was negatively correlated with PHD2 (r = -0.799, P = 0.00). HIF-1α was negatively correlated with VHL (r = -0. 625, P = 0.003).BWHR could effectively treat EM. Its mechanism might be associated with reducing contents of HIF-1α, serum CA125, and plasma cGMP, and up-regulating expressions of PHD2, VHL, and cAMP.

Chemotherapy is one of the most commonly used therapeutic strategies for metastatic colon cancer. However, the development of resistance to chemotherapeutic agents limits their application in clinical use. The underlying mechanisms of this resistance development require further elucidation. The current study investigated the effects of connexin43 (Cx43) gap junctions on 5‑fluorouracil (5‑FU), oxaliplatin and irinotecan in colon cancer cells. Three different methods were used to manipulate Cx43 gap junction function: i) Cell culture at different densities; ii) pretreatment with a Cx43 specific inhibitor or enhancer; and iii) Cx43 gene knock‑down. Results indicated that the cell toxicity of 5‑FU, oxaliplatin and irinotecan was cell density‑dependent, which was mediated by gap junctions. Downregulation of Cx43 gap junction functioning attenuated 5‑FU, oxaliplatin and irinotecan toxicity in colon cancer cells, which was increased in cells treated with a Cx43 gap junction function enhancer. Thus, the results of the present study suggest that resistance to 5‑FU, oxaliplatin and irinotecan in colon cancer cells was relative to Cx43 expression loss as cancer developed, which may indicate a novel basis for therapeutic strategy development to combat drug resistance in numerous cell types, in addition to colon cancer cells.

Linear Alkyl Benzene (LAB) is a promising liquid scintillator solvent in neutrino experiments because it has many appealing properties. The timing properties of LAB-based liquid scintillator have been studied through ultraviolet and ionization excitation in this study. The decay time of LAB, PPO and bis-MSB is found to be 48.6 ns, 1.55 ns and 1.5 ns, respectively. A model can describe the absorption and re-emission process between PPO and bis-MSB perfectly. The energy transfer time between LAB and PPO with different concentrations can be obtained via another model. We also show that the LAB-based liquid scintillator has good (n, γ) and (α, γ) discrimination power.

The product concept evaluation plays a very important role in product development. It can be considered as multi-criteria decision making (MCDM) problem. In a MCDM problem, a decision maker has to choose the best alternative that satisfies the evaluation criteria among a set of candidate solutions. This paper introduces a compromise MCDM evaluation model which combines fuzzy DEMATEL and VIKOR methods. The criteria weights are determined based on separate cause-effect assessment of a group of experts using the fuzzy DEMATEL approach. The resulting criteria weights are applied to the decision analysis where the fuzzy VIKOR approach is adopted which determines a compromise solution by using utility weight.

The oxygen quenching effect in Linear Alkl Benzne (LAB) based liquid scintillator (LAB as the solvent, 3 g/L 2, 5 diphe-nyloxazole (PPO) as the fluor and 15 mg/L $p$-bis-($o$-methylstyryl)-benzene (bis-MSB) as the $\lambda$-shifter) is studied by measuring the light yield as the function of the nitrogen bubbling time. It is shown that the light yield of the fully purged liquid scintillator is increased by 11% at the room temperature and the room atmosphere pressure. A simple nitrogen bubbling model is proposed to describe the relationship between the relative light yield (oxygen quenching factor) and the bubbling time., Comment: five pages,six figures

American Coherent Company. Compared the rvesult, we concluded it is better to incise the tissue with the ultrapulse CO2laser than that with the continue-wave CO2 laser. The former can prevent grunulation tissue over growing in the wound andinprove wound healing.Key words: belpharoplastry, laser, ultrapulesBelpharoplastry is a comman plastis operation. Transconjunctival beipharoplastry has developed widely with CO,laser or Nd: YAG laser. But some problems were common. Such as slow wonnd healing, grunulation tissue over growing inthe wound et a!. We selected younger cases for the lower tid transconjunctival beipharoplastry, and used ultrapulse CO2 laserin the openation. The result shows that this kind of laser can make wound healing faster and no grunulation tissue overgrowing in the wound. The result are report as follow:1. MATERIAL48 patients were engaged in this study. The age range from twenty to sisty-three. Twenty to twenty-nine 8 cases.Thirty to thirty-nine 26 cases. Forty to forty-nine 1 0 cases. 4 cases are over fifty. 44 cases are famale, the other 4 cases aremale. They were divided into A group and B group by ramdon. The follow is laser parameter settings. A group, pulseenergy: lSmj to 2Omj; pulse rate:. 150 HZ to 200HZ; the power 3w or 4w; the diameter of the focuse is 0.2mm. B group,select the continue-wave CO2 laser, the power is 3W or 4W; the diameter of focuse is 0.2mm. Except for two cases, thereare no flaccid skin of the lowe lid. The disease of conjunctiva and belpharoplastry were not found in all cases before. Photoswere taken before the operation.2. METHOD(1) Preparation: 0.5% Chloromycinum eye drops used three times daily for three days before operation.

There were one hundred Chinese cases treated with face laser resurfacing. Observed the result, the post-inflammatory hyperpigmentation was found in all case. It is not any use that the cases used the 3 percent hydroquinone before the operation. Eighty-five cases were going down between two and six months after the operation. Fifteen cases continued to nine months. Five cases continued to a year. Cosmetics are possible to affect the course of inflammatory hyperpigmentation. Hyperpigmentation disappeared more quickly while using 3 percent hydroquinone cream or KA cream after the operation. And intravenous injections with vitamin C is helpful.© (1998) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.