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Supplementary Table 1. Differentially Expressed Probesets comparing samples from ovarian cancer patients with chemotherapy treatment to samples without chemotherapy treatment

Purpose: We investigated the mechanisms of how TGFβ pathway is activated by chemotherapeutics and whether a novel TGFβ trap called RER can block chemotherapeutics-induced TGFβ pathway activation and enhance their antitumor activity in gynecologic cancer.Patients and Methods: An unbiased bioinformatic analysis of differentially expressed genes in 31 ovarian cases due to chemotherapy was used to identify altered master regulators. Phosphorylated Smad2 was determined in 30 paired cervical cancer using IHC. Furthermore, the effects of chemotherapeutics on TGFβ signaling and function, and the effects of RER on chemotherapy-induced TGFβ signaling were determined in gynecologic cancer cells.Results: Chemotherapy-induced transcriptome alteration in ovarian cancer was significantly associated with TGFβ signaling activation. Chemotherapy was found to activate TGFβ signaling as indicated by phosphorylated Smad2 in paired cervical tumor samples (pre- and post-chemotherapy). Similar to TGFβ1, chemotherapeutics were found to stimulate Smad2/3 phosphorylation, cell migration, and markers related to epithelial–mesenchymal transition (EMT) and cancer stem cells (CSC). These TGFβ-like effects were due to the stimulation of TGFβ1 expression and secretion, and could all be abrogated by TGFβ inhibitors including a novel TGFβ trap protein called RER both in vitro and in vivo. Importantly, combination treatment with RER and cisplatin showed a higher tumor inhibitory activity than either agent alone in a xenograft model of ovarian cancer.Conclusions: Chemotherapeutics can stimulate TGFβ1 production and consequently enhance TGFβ signaling, EMT, and CSC features resulting in reduced chemo-sensitivity. Combination therapy with a TGFβ inhibitor should alleviate this unintended side effect of chemotherapeutics and enhance their therapeutic efficacy. Clin Cancer Res; 24(12); 2780–93. ©2018 AACR.

Supplementary Figure 1. Chemotherapy-associated differentially expressed genes that are regulated by TGF-β. Supplementary Figure 2. Density analysis of P-Smad2 and P-Smad3 bands in Figure 3. The density of corresponding total Smad2 (T-Smad2) and total Smad3 (T-Smad3) bands were used for normalization. Supplementary Figure 3. HeLa and C-41cellswere treated with TGF-β1 (80pM), RER (40nM) or HTS (100nM) alone or in combination for 6 h. The cell extracts were used for Western immunoblotting analysis to measure the levels of P-Smad2, P-Smad3, T-Smad2, and T-Smad3 with their respective antibodies. Supplementary Figure 4. Forward and side scatter (FSC and SSC) density plots for excluding dead cells and debris. Supplementary Figure 5. OVCAR-3 cells were treated with TGF-β1 (80pM), cisplatin (1μM), HTS (100nM), or RER (40nM) alone or in combination for 4 days.

Background Ultrasound examination can be applied to the diagnosis of pediatric elbow fracture. This study aims to analyze the application value of ultrasound in the surgical treatment of supracondylar humeral fractures. Methods 64 children with supracondylar humeral fractures were treated with ultrasound-guided closed reduction and percutaneous pinning (CRPP), 31 patients were treated with CRPP under radiography guidence. The reduction effect of supracondylar humeral fractures was determined through the perioperative ultrasound images of the lateral, medial and posterior aspects of the elbow. Percutaneous pinning was performed after supracondylar humeral fractures were well reduced. A follow-up examination was performed and all the patients were evaluated according to Flynn’s criteria. Results The mean duration of surgery was 58.3 min (42–108 min) in the ultrasound group and 41.5 min (24-63 min) in the radiography group (P P > 0.05). After surgery, three patients had pin tract infection. One patient had ulnar nerve neurapraxia in the radiography group. No cases with Volkmann’s contracture were reported. Conclusion Ultrasound-guided CRPP is a safe and reliable surgical treatment of pediatric supracondylar humeral fractures. Trial registration Retrospectively registered.

Objective: To investigate the relationship between NLR(lymphocyte ratio), PLR(platelet to lymphocyte ratio), LMR(lymphocyte to monocyte ratio) and PNS(platelet to neutrophil ratio) and the lateral pillar classificationof Perthes disease, and to provide an alternative index for clinical diagnosis and prognosis. Methods: 59 children with Perthes disease and 60 children in the healthy control group without femoral head necrosis in our hospital from 2012 to 2021 were collected. The general data were collected. The modified herring lateral pillar classification was collectedfor the case group, and the inflammatory factors NLR, PLR, LMR and PNS were collected. The cases were divided into three groups, herring A and B were group I, herring B/C and C were group II, and the healthy control group was group III. The hematological indexes (NLR, PLR, LMR, PNS) of children at different stages were statistically analyzed. Results: Group I consisted of 36 patients, with an average age of 7.4 ± 2.0 years (3–11 years). Group II consisted of 23 patients, with an average age of 7.4 ± 1.9 years (4–12 years). Group III consisted of 60 patients, with a mean age of 7.4 ± 2.7 years (4–13 years). The average values of PLR in groups I, II and III were 131.98 ± 47.44, 122.19 ± 37.88 and 102.46 ± 30.68, respectively. It's worth noting that there was statistically significant difference among these groups (P=0.003). The optimal threshold of PLR was 130.25, the sensitivity was 45.8% and the specificity was 85%. Conclusion: PLR has certain value in the diagnosis of Perthes disease. The higher the value of PLR, the higher the risk of Perthes disease.

Carburizing is the main damage form of ethylene cracking furnace tubes. In this process, the microstructure of the furnace tube would change and the element diffuses and redistributes. After serving for about 41000h, the radiation section of a plum blossom tube of SC-1 tubular cracking furnace from a petrochemical company was tested and analyzed in this article. Results show that the higher the service temperature, the more serious the carburizing of the furnace tube. In the inner-wall carburized zone of the middle temperature section of the furnace tube with an initial C content of 0.1wt%, the maximum C content reaches 1.83wt% and the number of carbides increases obviously as well as its organizational morphology changes from fine granular to coarse block or chain like and its organizational type changes from single M23C6 to the coexistence of M23C6, M7C3 and MC type carbides. The Cr and C elements in the carburized zone are mainly concentrated in the grain boundary area in the form of carbides. At the same time, the diffusion of alloy elements causes Cr deficiency in the matrix, and the carbide deficiency zone appears in the subsurface of the inner wall.

In online systems, including e-commerce platforms, many users resort to the reviews or comments generated by previous consumers for decision making, while their time is limited to deal with many reviews. Therefore, a review summary, which contains all important features in user-generated reviews, is expected. In this article, we study “how to generate a comprehensive review summary from a large number of user-generated reviews.” This can be implemented by text summarization, which mainly has two types of extractive and abstractive approaches. Both of these approaches can deal with both supervised and unsupervised scenarios, but the former may generate redundant and incoherent summaries, while the latter can avoid redundancy but usually can only deal with short sequences. Moreover, both approaches may neglect the sentiment information. To address the above issues, we propose comprehensive Review Summary Generation frameworks to deal with the supervised and unsupervised scenarios. We design two different preprocess models of re-ranking and selecting to identify the important sentences while keeping users’ sentiment in the original reviews. These sentences can be further used to generate review summaries with text summarization methods. Experimental results in seven real-world datasets (Idebate, Rotten Tomatoes Amazon, Yelp, and three unlabelled product review datasets in Amazon) demonstrate that our work performs well in review summary generation. Moreover, the re-ranking and selecting models show different characteristics.

Revision operations have become a new issue after successful artificial joint replacements, and periprosthetic osteolysis leading to prosthetic loosening is the main cause of why the overactivation of osteoclasts (OCs) plays an important role. The effect of biochanin A (BCA) has been examined in osteoporosis, but no study on the role of BCA in prosthetic loosening osteolysis has been conducted yet. In this study, we utilised enzyme-linked immunosorbent assay, computed tomography imaging, and histological analysis. Results showed that BCA downregulated the secretion levels of tumor necrosis factor-α, interleukin-1α (IL-1α), and IL-1β to suppress inflammatory responses. The secretion levels of receptor-activated nuclear factor-κB ligand, CTX-1, and osteoclast-associated receptor as well as Ti-induced osteolysis were also reduced. BCA effectively inhibited osteoclastogenesis and suppressed hydroxyapatite resorption by downregulating OC-related genes in vitro. Analysis of mechanisms indicated that BCA inhibited the signalling pathways of mitogen-activated protein kinase (P38, extracellular signal-regulated kinase, and c-JUN N-terminal kinase) and nuclear factor-κB (inhibitor κB-α and P65), thereby downregulating the expression of nuclear factor of activated T cell 1 and c-Fos. In conclusion, BCA may be an alternative choice for the prevention of prosthetic loosening caused by OCs.

Bone metastases is a common manifestation of advanced malignant tumors. With the recent advances in medical technology, the survival period of patients with malignant tumors is prolonged, and the probability of bone metastases is significantly increased. Approximately 70% to 80% of patients with breast or prostate cancer will eventually develop bone metastases. In addition, thyroid, lung, and kidney carcinomas are all known to cause bone metastases, with a 30% to 40% incidence upon postmortem assessment. Bone metastases often lead to severe pain, pathological fractures, and nerve damage and have become a critical factor affecting the quality of life and life expectancy of cancer patients. Although treatments for bone metastases are diverse, choosing the appropriate treatment is difficult. Both conservative treatment and open surgery have certain drawbacks and may not be appropriate for all patients. Interventional procedures have the advantages of less trauma with quicker recovery and represent a viable alternative. This review provides updates on the progress of research on the interventional treatment of bone metastases and directions regarding relevant further studies.

Background To assess the associations between family characteristics and influenza vaccination coverage among children. Methods Data were used from the National Health Interview Survey 2011–2016. Results The number of children ranged from 10 720 to 12 991 per year from 2011 to 2016. After adjustment for the high-risk status of influenza complications, sex, age, region, race and office visits of children, children of adults who received influenza vaccination were 3.83–4.79 times (the lowest and the highest odds ratios from 2011 to 2016) more likely to be immunized for influenza. Health insurance (1.36–1.61) was also associated with vaccination in children. However, the education level of family adults (0.59–0.79) and marital status of being widowed/divorced/separated (0.74–0.79) were negatively associated with vaccination uptake in children. The following characteristics were not major concerns with influenza vaccination in children: class of worker, years on the job, physical activity, drinking, smoking, hepatitis B immunization, number of family members aged ≥65 years and the ratio of family income to the poverty threshold. Overall, results from the pooled analysis (2011–2016) were consistent with the above-mentioned findings. Conclusions Promoting parental influenza immunization and access to health insurance may be the most effective tools to promote the coverage of influenza vaccine among children.

Background Human papillomavirus (HPV) vaccination has been recommended for young adults aged 18-26 years. Methods Data from the 2016 and 2017 National Health Interview Survey were pooled. Results The prevalence of HPV vaccination initiation (≥1 dose) was 32.54% in 2016 and 37.92% in 2017, and the prevalence of HPV vaccination completion (≥3 doses) was 17.05% in 2016 and 19.38% in 2017 among young adults aged 18-26 years. Being female sex (odds ratio: 4.74) was the leading indicator for receiving the HPV vaccine, followed by receipt of other recommended vaccines (pneumococcal, hepatitis B, and hepatitis A), begin born in the United States, being gay/lesbian, having a greater number of physician visits, being younger, not being married, living in the Northeast, ever having had a pap smear, higher education, being not Hispanic ethnicity, and having more physical activity. Meanwhile, being female sex (odds ratio: 7.23) was the leading indicator for completing the HPV vaccination, followed by receipt of other recommended vaccines (pneumococcal, hepatitis B, and hepatitis A), having a greater number of physician visits, being younger, being born in the United States, being gay/lesbian, living in the Northeast, and current drinking. Conclusions HPV vaccination coverage remains low among young adults, and HPV vaccine coverage was associated with a number of characteristics.

Background The hepatitis B (HepB) vaccine is recommended for adults traveling to a country of high or intermediate endemicity. Methods Data from the 2016 and 2017 National Health Interview Surveys were pooled. Results The weighted prevalence of HepB vaccination initiation (≥1 dose) was 37.67% in 2016 (weighted number: 30,581,813/81,192,803) and 40.20% in 2017 (weighted number: 34,509,993/85,849,427). The weighted prevalence of HepB vaccination completion (≥3 doses) was 29.97% in 2016 (weighted number: 24,331,218/81,192,803) and 31.78% in 2017 (weighted number: 27,282,536/ 85,849,427). Characteristics independently associated with HepB vaccination initiation (in descending order by odds ratio) included age, receipt of influenza vaccine, education, ever having lived with someone with hepatitis, class of worker, number of physician visits in the past 12 months, ratio of family income to the poverty threshold, region, sexual orientation, gender, heath insurance, computer use, physical activity, and Hispanic ethnicity. Similar results were found in the analysis for HepB vaccination completion, except that subjects born in the United States showed a higher likelihood of HepB vaccination completion. Conclusions HepB vaccination initiation and completion were associated with a number of characteristics that can be utilized to develop strategies to increase HepB vaccination coverage among adults traveling to a country of high or intermediate endemicity.

Microbial community diversity and volatile profiles of 14 Jiang-flavor liquor Daqu (JFLQ) samples were investigated by Illumina MiSeq platform and Head Space-solid Phase Microextraction Gas Chromatograph Mass Spectrometer (HS-SPME-GC-MS). Correlations between microbial community and volatile profiles of JFLQ were disclosed by redundancy analysis. Results indicated the outstanding high content of nitrogenous and aromatics compounds in Moutai Daqu samples, whereas esters were dominant for the samples from the brewing sites rather than Moutai. Bacillales, Enterobacteriales, and Lactobacillales were the dominant bacteria, while Candida, Trichoderma, Aspergillus, Trichosporon and Thermomyces were predominant in the fungal community. Compared to the randomly dispersed feature of fungi communities, bacterial communities had preferred "shelter": Lactobacillales mainly clustered in the surface of Daqu, whereas Bacillales in the core. Microbial interaction of JFLQ was stronger in the core than in the surface. Bacillales and Lactobacillales were closely positive-related with pyrazines and esters respectively, and Aspergillus was correlated with pyrazines, esters and aromatics. Based on the results, a metabolic map of abundant microorganisms in JFLQ was built up.

The pathogenesis of Legg-Calvé-Perthes disease (LCPD) has not been fully elucidated, and studies on epigenetic changes that may contribute to the pathogenesis of LCPD are rare. MicroRNAs (miRNAs) are epigenetic modifications that play a critical role in gene regulation. This study aimed to determine the expression profiles of circulating exosomal miRNAs and examine the role of exosomal miRNAs in LCPD. Exosomes were extracted from the plasma of three patients with LCPD and three matched healthy volunteers. Total exosomal miRNAs were isolated, and next-generation sequencing and bioinformatic approaches were performed. The top 10 most differentially upregulated miRNAs were identified, and qRT-PCR validation was performed using additional 10 matches. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, plasma exosomes were used in verifying osteoclastogenesis and the endothelial dysfunction phenotypes involved. The elevated miRNAs in LCPD plasma exosomes were tested for osteoclastogenesis and endothelial dysfunction in vitro. Sequencing results revealed the expression profiles of plasma exosomal miRNAs with differential expression from the DESeq-identified miRNA profiles in LCPD versus controls in a pairwise comparison. Gene Ontology and KEGG pathway analyses indicated that the predicted target genes of different miRNAs were mainly enriched in the endothelial and osteoclast cells related to signaling pathways. Functional phenotype experiments showed that the plasma exosomes in the LCPD group promoted osteoclastogenesis and endothelial cell dysfunction. qRT-PCR experiments showed that nine miRNAs in circulating exosomes in LCPD patients were higher than those in the healthy controls. miR-3133, miR-4644, miR-4693-3p, and miR-4693-5p promoted endothelial dysfunction, and miR-3133, miR-4693-3p, miR-4693-5p, miR-141-3p and miR-30a promoted osteoclastogenesis in vitro. This study demonstrated that plasma exosomes from LCPD promote endothelial cell dysfunction and osteoclastogenesis likely through their miRNAs, which might contribute to the development of LCPD.

PurposeThe present study aimed to explore the influence of ulnar bow on the surgical treatment of Bado type I missed Monteggia fracture in children.MethodsThis study is a retrospective review of 24 patients between November 2010 and March 2019. All patients were treated with open reduction of the radial head and ulnar opening wedge osteotomy without annular ligament reconstruction. The mean interval between injury onset and surgery was five months (range: 2–12 months). The average age of participants at the time of surgery was 6.4 years (range: 3–10 years). We evaluated the maximum ulnar bow (MUB) and MUB position (P-MUB) via radiography. The patients were divided into middle group (group A: 14 cases, MUB located at 40% to 60% of the distal ulna) and distal group (group B: 10 cases, MUB located at 20% to 40% from the distal end of the ulna) based on P-MUB. The mean period of follow-up was 37 months (range: 6–102 months).ResultsAt the last follow-up, all the children showed stable reduction of the radial head, and the flexion function of elbow joint improved after operation (P2 =0.497,P=0.013), The osteotomy angle was positively correlated with the P-MUB (R2=0.731,P=0.000), The R-MUB is proportional to the P-MUB (R2 =0.597,P=0.002). The regression equation of P-MUB and osteotomy angle: Angle=7.064+33.227* P-MUB (R2=0.459, P =0.000).ConclusionWhen the ulnar bow is positioned at the middle ulna, a stable reduction of radial head need to be achieved through a larger angle in the ulnar osteotomy. If the position of maximum ulnar bow (P-MUB) is closer to the middle of the ulna or the ratio of maximum ulnar bow (R-MUB) is larger, the osteotomy angle is larger.

Aims Endothelial microparticles (EMPs) are extracellular vesicles secreted by endothelial cells. The purpose of this research is to explore that the clinical significance and roles in angiogenesis and endothelial dysfunction of circulating microparticles in Perthes disease. Main methods We collected platelet-poor plasma (PPP) from patients and controls, then microparticles (MPs) were extracted. Flow cytometry was performed to calculate the concentrations of CD31+/CD42b-, CD62E+ and CD31+/CD42b+ MPs. ELISA was performed to detect the expression level of biomarkers of endothelial dysfunction and inflammatory factors in plasma. In vitro experiments to evaluate the effect of circulating MPs and EMPs derived from IL-6-stimulated human umbilical vein endothelial cells (HUVECs) on angiogenesis and endothelial dysfunction. Key findings Our results revealed that the CD31+/CD42b- EMPs were significantly higher in Perthes disease group than in the control group. The Perthes-MPs being taken up by HUVECs promoted endothelial cell apoptosis, endothelial dysfunction and inhibited angiogenesis in vitro. Moreover, the level of IL-6 in plasma significantly increased in patients with Perthes, which was tightly correlated with the elevated level of circulating CD31+/CD42b- EMPs. IL-6 promoted HUVECs to secrete CD31+/CD42b- MPs, and EMPs derived from high concentration IL-6-stimulated (100 and 1000 pg/mL) HUVECs promoted endothelial cell apoptosis, endothelial dysfunction and inhibited angiogenesis. Significance In summary, our study suggests that circulating EMPs in the phenotypic spectrum revealed unique phenotypes of endothelial dysfunction, showing close correlation with the secretion of IL-6. These circulating EMPs may give rise to endothelial cell apoptosis, endothelial dysfunction and angiogenesis in Perthes disease.

Background To investigate the clinical imaging manifestations, diagnosis and treatment of intraperitoneal extraosseous osteosarcoma. Case presentation A 52-year-old male patient with intraperitoneal extraosseous osteosarcoma was retrospectively analyzed. He suffered from left lower abdominal pain accompanied by mass for 6 months. On abdominal CT scan, multiple patchy and banded calcification were found. The largest is about six centimeters in diameter and underwent mass resection. Postoperative pathology revealed retroperitoneal osteosarcoma. The reported intraperitoneal extraosseous osteosarcoma was analyzed and the related literature was reviewed. Two years after operation, the patients had recurrence of the tumors and invaded sigmoid colon, peritoneum and bladder. Palliative operation was performed to remove the tumors in the bladder and transverse colostomy was performed. The patients were followed up by telephone and died 2 months after the second operation. Conclusions Intraperitoneal extraosseous osteosarcoma has a low incidence and has no specific imaging features. Surgical resection is the main treatment and the prognosis is poor.

The electron beam smelting (EBS) is employed in refining the nickel-based superalloy. Removal efficiency of impurities and inclusions in nickel-based superalloy during EBS has been investigated. The morphology, microstructure and the content of oxygen in nickel-based superalloy before and after the experiment were analyzed. The EBS process is divided into three stages: melting stage, refining stage and solidification stage. The results show that the oxygen has been removed during the EBS process. The optimal experiment parameters are smelting power of 9 kW and smelting time of 30 min, by which a minimal evaporation loss of the alloy and a highest removal efficiency of impurities can be acquired. The removal efficiency of oxygen reaches 60% with the optimized experiment parameters. The inclusions are consist of oxides of aluminum, silicon and calcium and their composite oxides. The inclusions enrich at the circular region of the ingot periphery during solidification. A new method for preparation of nickel-based superalloy with high purity is found in the laboratory, and the enrichment mechanism of inclusion is analyzed.

In currently popular access control models, the security policies and regulations never change in the running system process once they are identified, which makes it possible for attackers to find the vulnerabilities in a system, resulting in the lack of ability to perceive the system security status and risks in a dynamic manner and exposing the system to such risks. By introducing the maximum entropy (MaxENT) models into the rule optimization for the Bell–LaPadula (BLP) model, this paper proposes an improved BLP model with the self-learning function: MaxENT-BLP. This model first formalizes the security properties, system states, transformational rules, and a constraint model based on the states transition of the MaxENT. After handling the historical system access logs as the original data sets, this model extracts the user requests, current states, and decisions to act as the feature vectors. Second, we use $k$ -fold cross validation to divide all vectors into a training set and a testing set. In this paper, the model training process is based on the Broyden–Fletcher–Goldfarb–Shanno algorithm. And this model contains a strategy update algorithm to adjust the access control rules dynamically according to the access and decision records in a system. Third, we prove that MaxENT-BLP is secure through theoretical analysis. By estimating the precision, recall, and F1-score, the experiments show the availability and accuracy of this model. Finally, this paper provides the process of model training based on deep learning and discussions regarding adversarial samples from the malware classifiers. We demonstrate that MaxENT-BLP is an appropriate choice and has the ability to help running information systems to avoid more risks and losses.

This study aimed to investigate the effects of different distillation patterns on main compounds of Chinese Luzhou-flavour liquor. Results showed that more volatiles of raw liquor might originate from fresh grains for a newly constructed pit. A modest (p = 0.05) increase in the total amount of volatiles was found in the heart liquor from fermented grains added to fresh grains and rice hulls to distillate (AG pattern), reaching a maximum value of 1012.22 mg/L. The percentage of esters in the AG pattern ranged from 81.22 to >95.03% compared with samples from fermented grains only added to rice hulls to distillate (NG pattern). Acids in the heart liquor obtained by the AG pattern were higher than those for NG, but the corresponding proportion was opposite. Alcohols were detected at ~1.62% in NG pattern but were 1.42% in AG. Cyanide and ethyl carbamate content in the head liquor were far higher than those in the heart and tail liquor. In particular, cyanide content dramatically increased after adding fresh grains during the distillation process. Copyright © 2017 The Institute of Brewing & Distilling

Everolimus inhibits mammalian target of rapamycin complex 1 (mTORC1) and is known to cause induction of autophagy and G1 cell cycle arrest. However, it remains unknown whether everolimus-induced autophagy plays a critical role in its regulation of the cell cycle. We, for the first time, suggested that everolimus could stimulate autophagy-mediated cyclin D1 degradation in breast cancer cells. Everolimus-induced cyclin D1 degradation through the autophagy pathway was investigated in MCF-10DCIS.COM and MCF-7 cell lines upon autophagy inhibitor treatment using Western blot assay. Everolimus-stimulated autophagy and decrease in cyclin D1 were also tested in explant human breast tissue. Inhibiting mTORC1 with everolimus rapidly increased cyclin D1 degradation, whereas 3-methyladenine, chloroquine, and bafilomycin A1, the classic autophagy inhibitors, could attenuate everolimus-induced cyclin D1 degradation. Similarly, knockdown of autophagy-related 7 (Atg-7) also repressed everolimus-triggered cyclin D1 degradation. In addition, everolimus-induced autophagy occurred earlier than everolimus-induced G1 arrest, and blockade of autophagy attenuated everolimus-induced G1 arrest. We also found that everolimus stimulated autophagy and decreased cyclin D1 levels in explant human breast tissue. These data support the conclusion that the autophagy induced by everolimus in human mammary epithelial cells appears to cause cyclin D1 degradation resulting in G1 cell cycle arrest. Our findings contribute to our knowledge of the interplay between autophagy and cell cycle regulation mediated by mTORC1 signaling and cyclin D1 regulation.

The aim of the study was to investigate the clinical outcomes of a combined anterior and posterior approach for the surgical treatment of chronic Monteggia fractures in children.From November 2010 to January 2018, 33 patients (27 boys and 6 girls) with chronic Monteggia fracture who were treated surgically by one surgeon of our department were retrospectively analyzed. In the surgical procedure, open reduction and excision of fibrous scar were performed with the anterior Henry's approach, while ulnar osteotomy was carried out with a posterior approach. In cases of unstable radial head reduction, a trans-capitellar K wire was applied. Repair or reconstruction of the annular ligament (ALR) was not undertaken.The average follow-up of the patients was 33.8 months (range 8-87 months). At the last follow-up, Mayor Score and function of flexion and extension showed significant improvement compared to preoperative condition (p 0.05). Two patients with palsy of the deep branch of the radial nerve with neurolysis recovered to normal over a 3-month follow-up. Redislocation occurred in two patients while subluxation occurred in one. One patient suffered a mild ischemic contracture but gradually recovered. Other severe complications, nerve injuries, heterotopic ossification, or synostosis, were not noted in the follow-up.A combined anterior and posterior approach for surgery resulted in a satisfactory outcome due to the advantages of better exposure, more convenient intraoperative management, and facilitate for radial nerve exploration. Our study provided a new approach for the surgery of chronic Monteggia fractures.

This study aimed to investigate volatile compounds characteristics of Pit mud (PM), Zaopei (ZP), Huangshui (HS) in Chinese Luzhou-flavor liquor fermentation cellar by HS-SPME/GC/MS. Results indicated that pit age and spatial position had a stronger effect on volatiles than physicochemical properties. A total of 59 volatiles had been identified, and phenylethyl alcohol, butyric acid, pentanoic acid, hexanoic acid, heptanoic acid, ethyl lactate, ethyl hexanoate, ethyl hexadecanoate, ethyl linoleate and ethyl elaidate were dominant, accounting for roughly 66.30%. Additionally, the total level of volatiles significantly increased from upper layer (U) to under layer (D), except for 2-year-MPM and 40-year-MZP, and 40-year-DPM (834.14 mg/kg) had the highest content. PCA explanation plane primarily revealed the main differentiation based on their loading plot values. Results of PLS-DA showed 4-methylphenol and gamma-nonanolactone were correlated with 2-year-PM. In addition to ethyl phenylacetate, ethyl hexanoate, furfuryl hexanoate, ethyl nonanoate, perhydrofarnesyl acetone and 2-acetyl furan were associated with 40-year-PM. Isoamyl hexanoate, octyl octanoate, benzaldehyde and 2-nonenal were with 10-year-PM. 3-methyl-1-butanol, ethyl lactate, ethyl hydrocinnamate and 3-furancarboxaldehyde were related to 2-year-ZP, while ethyl decanoate and ethyl linolenate were relevant to 10-year-ZP. No specific compounds for 40-year-ZP and HS samples were found.

The aim of this study was to assess the association of 27-bp variable number tandem repeat (VNTR) polymorphism in intron 4 and G894T polymorphism in exon 7 of the endothelial nitric oxide synthase (eNOS) gene with Legg-Calvé-Perthes disease (LCPD), and to provide a scientific basis for further research into the pathogenic mechanism. A total of 80 patients with LCPD and 100 healthy subjects were recruited in this case-control study. The 27-bp VNTR and G894T polymorphisms of the eNOS gene were genotyped using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism, respectively, followed by agarose gel electrophoresis and DNA sequencing. Allelic and genotypic frequencies were computed in the two groups and subjected to statistical analysis. For the 27-bp VNTR polymorphism, individuals with LCPD showed a higher frequency of the ab genotype [27.5 vs. 14%; odds ratio (OR), 2.33; 95% confidence interval (CI), 1.10–4.92; P=0.024]. For the G894T polymorphism, the LCPD case group showed a higher frequency of the heterozygous genotype GT than the healthy control group (35 vs. 17%; OR, 2.67; 95% CI, 1.33–5.36; P=0.005). The results indicate that these eNOS gene polymorphisms may be a risk factor for LCPD. The 27-bp VNTR polymorphism in intron 4 and G894T polymorphism in exon 7 may be involved in the etiology of LCPD.

Prosthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear-particle-induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle-induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor-κB (NF-κB) ligand-stimulated osteoclastogenesis, hydroxyapatite resorption, F-actin formation, and the gene expression of osteoclast-related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF-κB and mitogen-activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c-Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF-κB rhoifolin could decrease the number of tartrate-resistant acid phosphate-positive osteoclasts and titanium particle-induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts-stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.

Many consumers resort to others' assessments in product reviews for decision making, while their time is limited to deal with many reviews. Therefore, an expert's review which contains all important features in user-generated reviews, is strongly expected. In this paper, we study "how to generate expert's review from a large number of user-generated reviews (i.e., the crowds)." It can be implemented by text summarization, which mainly has two types of the extractive and the abstractive approaches. However, the former may generate redundant and incoherent summaries, while the latter cannot deal with long sequences. Moreover, both approaches usually neglect the sentiment information. To address the above issues, we propose a novel Expert Review Generation model to integrate a multi-attention mechanism with the encoder-decoder framework. We design a comprehensive preprocess strategy to identify the important sentences while keeping users' sentiment in original reviews, and use them as the input of the encoder-decoder generation model, so as to generate non-redundant and coherent summaries. Experimental results in two real world data sets (Idebate and Rotten Tomatoes) demonstrate that our model performs well in expert review generation.

Purpose: We investigated the mechanisms of how TGFβ pathway is activated by chemotherapeutics and whether a novel TGFβ trap called RER can block chemotherapeutics-induced TGFβ pathway activation and enhance their antitumor activity in gynecologic cancer. Patients and Methods: An unbiased bioinformatic analysis of differentially expressed genes in 31 ovarian cases due to chemotherapy was used to identify altered master regulators. Phosphorylated Smad2 was determined in 30 paired cervical cancer using IHC. Furthermore, the effects of chemotherapeutics on TGFβ signaling and function, and the effects of RER on chemotherapy-induced TGFβ signaling were determined in gynecologic cancer cells. Results: Chemotherapy-induced transcriptome alteration in ovarian cancer was significantly associated with TGFβ signaling activation. Chemotherapy was found to activate TGFβ signaling as indicated by phosphorylated Smad2 in paired cervical tumor samples (pre- and post-chemotherapy). Similar to TGFβ1, chemotherapeutics were found to stimulate Smad2/3 phosphorylation, cell migration, and markers related to epithelial–mesenchymal transition (EMT) and cancer stem cells (CSC). These TGFβ-like effects were due to the stimulation of TGFβ1 expression and secretion, and could all be abrogated by TGFβ inhibitors including a novel TGFβ trap protein called RER both in vitro and in vivo. Importantly, combination treatment with RER and cisplatin showed a higher tumor inhibitory activity than either agent alone in a xenograft model of ovarian cancer. Conclusions: Chemotherapeutics can stimulate TGFβ1 production and consequently enhance TGFβ signaling, EMT, and CSC features resulting in reduced chemo-sensitivity. Combination therapy with a TGFβ inhibitor should alleviate this unintended side effect of chemotherapeutics and enhance their therapeutic efficacy. Clin Cancer Res; 24(12); 2780–93. ©2018 AACR.

Prostate-specific antigen (PSA) biomarker assays are the current clinical method for mass screening of prostate cancer. However, high false-positive rates are often reported due to PSA's low specificity, leading to an urgent need for the development of a more specific detection system independent of PSA levels. In our previous research, we demonstrated the feasibility of using cellular refractive indices (RI) as a unique contrast parameter to accomplish label-free detection of prostate cancer cells via variance testing, but were unable to determine if a specific cell was cancerous or noncancerous. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free imaging system, which allows for the detection of individual prostate cancer cells utilizing cellular RI as the only contrast parameter. Noncancerous prostate (BPH-1) cells and prostate cancer (PC-3) cells were mixed at varied ratios and measured concurrently. Additionally, we isolated and induced PC-3 cells to undergo epithelial-mesenchymal transition (EMT) by exposing these cells to soluble factors such as TGF-01. The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced PC-3 cells as well as BPH-1 cells. EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. Our study shows promising clinical potential in utilizing the PC-TIR biosensor imaging system to not only detect prostate cancer cells, but also evaluate prostate cancer progression.

UNLABELLED The aim of this study was to investigate the dynamic of volatile compounds in the Zaopei during the fermentation and distillation process by headspace solid-phase microextraction-gas chromatography mass spectrometry (HS-SPME-GCMS). Physicochemical properties analysis of Zaopei (fermented grains [FG], fermented grains mixed with sorghum [FGS], streamed grains [SG], and streamed grains mixed with Daqu [SGD]) showed distinct changes. A total number of 66 volatile compounds in the Zaopei were identified, in which butanoic acid, hexanoic acid, ethyl hexanoate, ethyl lactate, ethyl octanoate, hexyl hexanoate, ethyl hydrocinnamate, ethyl oleate, ethyl hexadecanoate, and ethyl linoleate were considered to be the dominant compounds due to their high concentrations. FG had the highest volatile compounds (112.43 mg/kg), which significantly decreased by 17.05% in the FGS, 67.12% in the SG, and 73.75% in the SGD. Furthermore, about 61.49% of volatile compounds of FGS were evaporated into raw liquor, whereas head, heart, and tail liquor accounted for 29.84%, 39.49%, and 30.67%, respectively. Each volatile class generally presented a decreasing trend, except for furans. Especially, the percentage of esters was 55.51% to 67.41% in the Zaopei, and reached 92.60% to 97.67% in the raw liquor. Principal component analysis based ordination of volatile compounds data segregated FGS and SGD samples. In addition, radar diagrams of the odor activity values suggested that intense flavor of fruit was weakened most from FG to SGD. PRACTICAL APPLICATION The dynamic of volatile compounds in the Zaopei during the fermentation and distillation process was tested by SPME-GCMS. The result of this study demonstrated that both volatile compounds of Zaopei and thermal reaction during distillation simply determined the unique feature of raw liquor. This study was conducted based on the real products from liquor manufactory, so it is practicable that the method can be used in an industry setting.

In view of the equilibrium of fermentation rate in the cellar mainly depends on interphase microflora, it is essential to exploit interphase microbial community characteristics to understand the metabolic regulation mechanism. The objective of this study was to investigate interphase microbial community structure in the fermentation cellar of Chinese Luzhou-flavor liquor via combining phospholipid fatty acids (PLFAs) and denaturing gradient gel electrophoresis (DGGE). The total microbial biomass analyzed by PLFA in the pit mud (PM: 25.52–103.38 nmol/g) and Zaopei (ZP: 29.96–64.50 nmol/g) was obviously higher than Huangshui (HS: 4.35–7.82 nmol/g), and was strongly correlated with pit age, which was validated by DGGE results. Among the specific microbial groups, ‘gram-positive bacteria, anaerobe bacteria and fungi’ dominated in the PM, while ‘gram-positive bacteria, aerobe bacteria and fungi’ predominated in the ZP and HS, respectively. Principal component analysis (PCA) based ordination of the relative PLFA abundance data segregated PM, ZP and HS. Eubacterial PCR-DGGE illustrated that a total of 31 bands belonged to five families (Lactobacillaceae, Clostridiaceae, Porphyromonadaceae, Synergistaceae and Acetobacteraceae) were identified, and 41.52% eubacteria were affiliated with Lactobacillaceae in the ZP, while 44.67% and 40.41% eubacteria were affiliated with Clostridiaceae in the PM and HS, respectively. Archaeal PCR-DGGE analysis demonstrated that Methanosaeta, Methanocorpusculum, Methanobrevibacter, Methanobacterium and Methanoculleus were the majority of archaea. Particularly, Methanosaeta, as acetrophic methanogens, increased gradually in the PM and HS with pit age, and decreased in the ZP, and no family Methanobacteriaceae was detected in the 30-year-PM. Furthermore, fungal community was dominated by genus Pichia.

Latent Dirichlet Allocation (LDA), has been recently used to automatically generate text corpora topics, and applied to sentences extraction based multi-document summarization algorithms. In this paper, we propose a novel approach to automatic generation of aspect-oriented summaries from multiple documents. Our approach is to combine the traditional summary generation algorithm and the the abstract generation algorithm based on deep learning.We employ the improved traditional summary generation algorithm to convert multiple documents into a single document, and then using the resulting single document with the deep learning method to extract the final summary. At first, we apply improved LDA model to cluster sentences in all documents. Second, We employ the extended LexRank algorithm to sort the sentences in each cluster. Third, we use extended Hedge Trimmer algorithm for sentence compression. Fourth, We apply Integer Linear Programming for sentence selection, and in this step ,we get the single document. Finally, We employ the textum on TensorFlow to get the final abstract. The experiments showed that the proposed algorithm achieved better performance compared the other state-of-the-art algorithms on DUC2005 and TAC2010 corpus.