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Background Hepatic echinococcosis (HE) is a zoonotic disease caused by Echinococcus, and Echinococcus granulosus and Echinococcus multilocularis are the most common, causing cystic echinococcosis (CE) and alveolar echinococcosis (AE) respectively. Contrast-enhanced ultrasound (CEUS) is a new imaging technique which has been recommended for identifying focal lesions in the liver. However, the effect of CEUS on the differentiation of hepatic echinococcosis subtype remains unclear. Methods Twenty-five patients with 46 HE lesions confirmed by histopathology in our Hospital from December 2019 to May 2022 were reviewed by conventional ultrasound (CUS) and CEUS examinations respectively. The images and clips of the lesions by CUS and CEUS were reviewed retrospectively. The lesions by CUS were evaluated including the location, size, morphology, margin, internal echogenicity and the internal Dopper’s signal. The lesions by CEUS were evaluated including the enhancement degree, enhancement pattern and enhancing boundary in different phases. The diagnoses of lesions by CUS or CEUS were recorded respectively. By taking the histopathology as the gold standard, the paired X2 test was performed with statistical software (SPSS, Inc., Chicago, IL) and the results of differentiation of the subtype of HE by CUS and CEUS were statistically analyzed. Results A total of 46 lesions were involved in 25 patients, including 10 males (40.0%) and 15 females (60.0%) with aged 15 to 55 (42.9 ± 10.3) years. By histopathology, 9 patients with 24 lesions were diagnosed as CE and 16 patients with 22 lesions were diagnosed as AE. Among the 46 lesions of HE, compared with histopathological examination, the accuracy rate was 65.2% and 91.3% in CUS and CEUS findings respectively. Among the 24 CE lesions, 13 lesions were correctly differentiated by CUS, while 23 by CEUS. The difference between CUS and CEUS was statistically significant (Chi-square test, X2 = 8.10, df = 23, P < 0.005). Among the total 46 HE lesions, 30 lesions were correctly differentiated by CUS, while 42 by CEUS. The difference between CUS and CEUS was statistically significant (Chi-square test, X2 = 10.08, df = 45, P < 0.005). Conclusions CEUS is a more valid technique that can differentiate the subtype of HE from CE or AE than CUS. It could be a reliable tool in the differentiation of HE.

Adsorption and transport of levofloxacin (LEV) and ofloxacin (OFL) enantiomers in a matrix containing goethite and natural organic matter (NOM) were investigated using batch and column experiments. In batch studies, competition and enantioselectivity were observed in the adsorption of LEV and OFL. Enantioselectivity upon adsorption was investigated by comparing changes in the enantiomer fraction (EF) (the ratio of LEV to the sum of LEV and OFL remaining in the solution) after and before adsorption. At pH < 7, there was hardly any selectivity in adsorption of OFL and LEV to goethite. At pH > 7, OFL showed a stronger adsorption than LEV to goethite, and this preference remained when NOM samples of Leonardite humic acid (LHA) and Elliott Soil fulvic acid (ESFA) were added. However, when Suwannee River NOM (SRNOM) was added, the preference was reversed, and LEV was adsorbed more strongly. In single systems, the presence of different types of NOM increased adsorption of LEV and OFL, especially LEV. In column studies, preloaded NOM decreased the transport of LEV and OFL through goethite-coated sand. The EF values in the effluent increased with retention time and reached the largest values (0.59–0.72) at around 1.5 pore volume (PV), and then decreased again, reaching a stable value at 5.0–30.0 PV. Both batch and column experiments showed that, fractionation of LEV and OFL occurred during adsorption and transport in the presence of NOM-goethite complexes, which would eventually affect their environmental fate

There have been few studies on the cosmetic efficacy of Coprinus comatus, Cordyceps militaris, and Pleurotus geesteranus. This article aims to compare the cosmetic effects of the mycelium, fermentation broth, and mycelium and fermentation broth mixture of liquid-fermented C. comatus, C. militaris, and P. geesteranus. Our results verified that these three edible mushrooms all showed excellent antioxidant, moisturizing, and whitening effects. Comparatively, we found that the cosmetic effects of C. comatus were superior to C. militaris and P. geesteranus. Therefore, we speculate that among the three edible mushrooms, C. comatus will be the most suitable to be used as a basis for cosmeceutical formulations to tackle skin aging and hyperpigmentation. This research aims to provide a basis for the study of the cosmetic effects of edible mushrooms.

Coprinus comatus, an edible and medicinal mushroom, not only tastes delicious, but also has various pharmacological activities. Recently, it has been reported that researchers have extracted more and more active ingredients, including polysaccharides, comatin, active protein complexes, and phenols from fruit bodies, mycelium, or fermentation liquor of C. comatus and studied their corresponding functions. At present, researchers mainly focus on the hypoglycemic effect of C. comatus, while other effects are less studied. This paper summarizes not only the hypoglycemic effect of C. comatus, but also other functions, such as antioxidant activity, alcohol liver protection, cancer inhibition, antiandrogenic function, anti-inflammatory effect, treatment of leukemia, and so on, which will provide scientific basis for the deep processing and comprehensive utilization of C. comatus.

In order to help pathologists quickly locate the lesion area, improve the diagnostic efficiency, and reduce missed diagnosis, a convolutional neural network algorithm for the optimization of emergency nursing rescue efficiency of critical patients was proposed. Specifically, three convolution layers and convolution kernels of different sizes are used to extract the features of patients’ posture behavior, and the classifier of patients’ posture behavior recognition system is used to learn the feature information by capturing the nonlinear relationship between the features to achieve accurate classification. By testing the accuracy of patient posture behavior feature extraction, the recognition rate of a certain action, and the average recognition rate of all actions in the patient body behavior recognition system, it is proved that the convolution neural network algorithm can greatly improve the efficiency of emergency nursing. The algorithm is applied to the patient posture behavior detection system, so as to realize the identification and monitoring of patients and improve the level of intelligent medical care. Finally, the open source framework platform is used to test the patient behavior detection system. The experimental results show that the larger the test data set is, the higher the accuracy of patient posture behavior feature extraction is, and the average recognition rate of patient posture behavior category is 97.6%, thus verifying the effectiveness and correctness of the system, to prove that the convolutional neural network algorithm has a very large improvement of emergency nursing rescue efficiency.

Polysaccharides were one of the important active ingredients of medical fungi, on account of its unique role caused the widespread concern of researchers at home and abroad. Currently, the structure and antioxidant activity of Coprinus comatus (C. comatus) fruit bodies polysaccharides have been reported. However, there is no article on the simultaneous study of intracellular and extracellular polysaccharides of liquid fermented C. comatus (IPs, EPs) so far. Hence, this paper innovatively investigated the monosaccharide compositions, antioxidative activities and inhibition of α-amylase activity in vitro of IPs and EPs. In this study, we adopted the PMP pre-column derivatization combined with HPLC method to determine the monosaccharide compositions of the IPs and EPs. Besides, the total antioxidant capacity, DPPH radical scavenging capacity, ·OH radical scavenging ability and inhibition effect on α-amylase in vitro of crude polysaccharides (ICPs, ECPs) and deproteinized polysaccharides (IDPs, EDPs) were severally compared. Only three kinds of monosaccharides were detected in IPs. Among the monosaccharides tested, the EPs contained five kinds, of which galactose and xylose accounted for a large proportion. ICPs showed better antioxidant activity and inhibition of α-amylase activity. This article innovatively studied the IPs and EPs, which could lay the foundation for the deep development of C. comatus.

Background Lung cancer is the main cancer-related deaths worldwide. In this study, we explored the clinical prognostic significance and functional role of miR-939-3p in lung cancer. Methods We analyzed the expression of miR-939-3p in lung cancer tissues and cells by qRT-PCR. The prognostic significance of miR-939-3p was investigated using the Kaplan-Meier survival and Cox regression analyses. The CCK-8 assay was used to determine the role of miR-939-3p in cell proliferation. Transwell assays were used to determine the effects of miR-939-3p on cell migration and invasion abilities. Results The expression of miR-939-3p was upregulated in cancer tissues and cell lines compared with adjacent normal tissues and normal cells, respectively. The upregulated miR-939-3p was significantly associated with lymph node metastasis, TNM stage and poor prognosis of lung cancer patients. After the transfection of miR-939 mimic, overexpression of miR-939-3p promoted lung cancer cell proliferation, migration, and invasion. Conclusion These findings suggested that miR-939-3p acts as an oncogene and promotes cell proliferation, migration, and invasion in lung cancer. miR-939-3p may be a potential independent prognostic biomarker in lung cancer.

Environmental contextThe concentration, types and distribution of antibiotics in soils can have environmental effects and can be modelled using laboratory systems. Adsorption of ofloxacin (OFL) and levofloxacin (LEV) enantiomers to goethite can probe this behaviour and each binds differently to the solid phase. The different behaviour of LEV and OFL in relation to solid-solution partitioning will affect their environmental fate. AbstractThe adsorption of ofloxacin enantiomers, namely levofloxacin (LEV) and ofloxacin (OFL), to goethite was investigated using batch experiments. Structural information of aqueous and adsorbed LEV or OFL was obtained with ultraviolet–visible (UV-Vis), three-dimensional excitation–emission matrix (EEM) and attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopic methods. The results indicated that LEV molecules formed a bridging bidentate complex (≡(FeO)2–LEV) with the surface of goethite, and OFL formed a monodentate complex (≡FeO–OFL). The adsorption of OFL to goethite was stronger than that of LEV, owing to differences in their physicochemical properties and bonding modes. The adsorption of LEV and OFL to goethite in single systems was well simulated using the charge distribution multi-site complexation (CD-MUSIC) model, but their adsorption in the LEV–OFL–goethite systems was overestimated at pH ~5.2 and high concentrations of LEV–OFL mixture (19.59μM), in which the predicted amounts of adsorbed LEV and OFL were higher (20.0, 30.8%) than the experimental results. Compared with the unprotonated LEV or OFL, the protonated (>99.9%) ones were mainly adsorbed to the surface of goethite, and the single species may be used during their following modelling.

A simple and rapid method was developed for the analysis of ofloxacin enantiomers by chiral ligand exchange-high performance liquid chromatography (CLE-HPLC). In addition, the influences of common cations (Ca2+, Mg2+, Fe3+, Zn2+) and the content of humic acid (HA) on the separation were investigated. The separation was carried out on a C18 column (25 cm×0.46 cm, 5 μm). The mobile phase was 20% (v/v) methanol aqueous solution containing 4 mmol/L L-isoleucine (as ligand) and 3 mmol/L CuSO4. The pH of the mobile phase was 4.5, and the flow rate was 1.0 mL/min. The column temperature was 40℃, and the detection wavelength was 293 nm. The ofloxacin enantiomers (ofloxacin and levofloxacin) were separated within 18 min and the resolution (R) was 2.70. The results showed that metal cations and HA had no significant influence on the resolution of ofloxacin enantiomers. However, the measured peak area of ofloxacin enantiomers decreased particularly in presence of Fe3+ and on increasing HA content. The proposed method can rapidly and efficiently determine ofloxacin and its chiral isomers in surface water, the influences of Fe3+ and HA content should be considered for verifying the applicability of the method.